PLP1 (NM_000533) Human Untagged Clone

SKU SC119823

PLP1 (untagged)-Human proteolipid protein 1 (PLP1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PLP1
• Synonyms: GPM6C; HLD1; MMPL; PLP; PLP/DM20; PMD; SPG2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119823 sequence for NM_000533 edited (data generated by NextGen Sequencing); ATGGGCTTGTTAGAGTGCTGTGCAAGATGTCTGGTAGGGGCCCCCTTTGCTTCCCTGGTG; GCCACTGGATTGTGTTTCTTTGGGGTGGCACTGTTCTGTGGCTGTGGACATGAAGCCCTC; ACTGGCACAGAAAAGCTAATTGAGACCTATTTCTCCAAAAACTACCAAGACTATGAGTAT; CTCATCAATGTGATCCATGCCTTCCAGTATGTCATCTATGGAACTGCCTCTTTCTTCTTC; CTTTATGGGGCCCTCCTGCTGGCTGAGGGCTTCTACACCACCGGCGCAGTCAGGCAGATC; TTTGGCGACTACAAGACCACCATCTGCGGCAAGGGCCTGAGCGCAACGGTAACAGGGGGC; CAGAAGGGGAGGGGTTCCAGAGGCCAACATCAAGCTCATTCTTTGGAGCGGGTGTGTCAT; TGTTTGGGAAAATGGCTAGGACATCCCGACAAGTTTGTGGGCATCACCTATGCCCTGACC; GTTGTGTGGCTCCTGGTGTTTGCCTGCTCTGCTGTGCCTGTGTACATTTACTTCAACACC; TGGACCACCTGCCAGTCTATTGCCTTCCCCAGCAAGACCTCTGCCAGTATAGGCAGTCTC; TGTGCTGATGCCAGAATGTATGGTGTTCTCCCATGGAATGCTTTCCCTGGCAAGGTTTGT; GGCTCCAACCTTCTGTCCATCTGCAAAACAGCTGAGTTCCAAATGACCTTCCACCTGTTT; ATTGCTGCATTTGTGGGGGCTGCAGCTACACTGGTTTCCCTGCTCACCTTCATGATTGCT; GCCACTTACAACTTTGCCGTCCTTAAACTCATGGGCCGAGGCACCAAGTTCTGA; ; Clone variation with respect to NM_000533.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000533 unedited; GTCGCATTTGTAAACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGGCACGAGGC; AAAGATACTCAGAGAGAAAAATAAAAGACCGAAGAAGGAGGCTGGAGAGACCAGGATCCT; TCCAGCTGAACAAAGTCAGCCACAAAGCAGACTAGCCAGCCGGCTACAATTGGAGTCAGA; GTCCCAAAGACATGGGCTTGTTAGAGTGCTGTGCAAGATGTCTGGTAGGGGCCCCCTTTG; CTTCCCTGGTGGCCACTGGATTGTGTTTCTTTGGGGTGGCACTGTTCTGTGGCTGTGGAC; ATGAAGCCCTCACTGGCACAGAAAAGCTAATTGAGACCTATTTCTCCAAAAACTACCAAG; ACTATGAGTATCTCATCAATGTGATCCATGCCTTCCAGTATGTCATCTATGGAACTGCCT; CTTTCTTCTTCCTTTATGGGGCCCTCCTGCTGGCTGAGGGCTTCTACACCACCGGCGCAG; TCAGGCAGATCTTTGGCGACTACAAGACCACCATCTGCGGCAAGGGCCTGAGCGCAACGG; TAACAGGGGGCCAGAAGGGGAGGGGTTCCAGAGGCCAACATCAAGCTCATTCTTTGGAGC; GGGTGTGTCATTGTTTGGGAAAATGGCTAGGACATCCCGACAAGTTTGTGGGCATCACCT; ATGCCCTGACCGTTGTGTGGCTCCTGGTGTTTGCCTGCTCTGCTGTGCCTGTGTACATTT; ACTTCAACACCTGGACCACCTGCCAGTCTATTGCCTTCCCCAGCAGACCTCTGCCAGTAT; AGGCAGTCTCTGTGCTGATGCCNAGATGTATGGGTGTTCTCCCATGGAATGGCTTCCCTG; GCAGGGTTNTGTGCTNCNNACTTCTGTNCATCTGCAAAACAGCTGAGTTCCAAATGACCC; TTCCNACTGTTTATGCTGCATTTGTGGGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000533 unedited; GGTATGGCAACTTCCAGGGCCGGNANAGCACTGGGGAGGGGTCACAGGNATGCCACCCGG; GATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTT; TTTTTTGATTGAGAACATTCTTAATTTTATTTTCTTTAATTTTATAAAATACACTTTGTA; AGATAAGTTTCTAAAAGTTTATCCTTTATGTGTGTTAAAATTGCAATTCTATATCAGAAA; TGAAGGAAACACTTTCAGTTGATTAACTCTCTTTGTGTGTGTATATATGTGTATGTATGC; ATGTGAGGTTTTCAGGGAAGGGTTGGTTATTTGTTATGTTATTAAATCAAAACAAAACAC; AAGGTACGGATTACTTCACTGTCCTTTATCTTGATTGGTTTCTTACAAAACATTTTCCCT; CTCCTCCTTCTATGCAGCCTGGAAGCTTATCTTGTATACTGGTTTGAAAACAAGTATCAA; GTGTCTTTTTGTAAAGCTCTTACATCTCCTTAAATATTGCTGAAACCACTTTGGGGGAAA; AGAACAACACAACTCTTTACAACAAAAGAACTGTAGTACAAATCTTTCCTTCAATTAAAA; CCAATCAGACTTTTTTTTTTTTTAACCCAAAGTTAACAAAAACAGGAAAAGACTGAAATC; TGGGAGCTATTCAGGTCTCAAACTCTTTCTGCCTGTCCTTTTTCCTGGAAGCTTACCAAC; TGAATAGCTGATGACTGGTGCATTTCTGTATGTGATCTATATCAGGAGAAAATACCTTTA; TGNTAAACCTACTCTTACACACCAGATACATTGCTAATAAATCATTTTCTTAGCTAGAAG; ACGATCGATACTCAATGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_000533
• Insert Size: 834 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000533.3, NP_000524.3
• RefSeq Size: 3038 bp
• RefSeq ORF: 834 bp
• Locus ID: 5354
• UniProt ID: P60201
• Cytogenetics: Xq22.2
• Domains: Myelin_PLP
• Protein Families: Druggable Genome, Transmembrane
• Summary: This gene encodes a transmembrane proteolipid protein that is the predominant component of myelin. The encoded protein may play a role in the compaction, stabilization, and maintenance of myelin sheaths, as well as in oligodendrocyte development and axonal survival. Mutations in this gene cause Pelizaeus-Merzbacher disease and spastic paraplegia type 2. Alternatively splicing results in multiple transcript variants, including the DM20 splice variant. [provided by RefSeq, Feb 2015]; Transcript Variant: This variant (1, also known as PLP) encodes the longest isoform (1). Variants 1 and 3 encode the same isoform (1).