Ornithine Carbamoyltransferase (OTC) (NM_000531) Human Untagged Clone

SKU SC119821

OTC (untagged)-Human ornithine carbamoyltransferase (OTC), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: Ornithine Carbamoyltransferase
• Synonyms: OCTD; OTCD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119821 sequence for NM_000531 edited (data generated by NextGen Sequencing); ATGCTGTTTAATCTGAGGATCCTGTTAAACAATGCAGCTTTTAGAAATGGTCACAACTTC; ATGGTTCGAAATTTTCGGTGTGGACAACCACTACAAAATAAAGTGCAGCTGAAGGGCCGT; GACCTTCTCACTCTAAAAAACTTTACCGGAGAAGAAATTAAATATATGCTATGGCTATCA; GCAGATCTGAAATTTAGGATAAAACAGAAAGGAGAGTATTTGCCTTTATTGCAAGGGAAG; TCCTTAGGCATGATTTTTGAGAAAAGAAGTACTCGAACAAGATTGTCTACAGAAACAGGC; TTTGCACTTCTGGGAGGACATCCTTGTTTTCTTACCACACAAGATATTCATTTGGGTGTG; AATGAAAGTCTCACGGACACGGCCCGTGTATTGTCTAGCATGGCAGATGCAGTATTGGCT; CGAGTGTATAAACAATCAGATTTGGACACCCTGGCTAAAGAAGCATCCATCCCAATTATC; AATGGGCTGTCAGATTTGTACCATCCTATCCAGATCCTGGCTGATTACCTCACGCTCCAG; GAACACTATAGCTCTCTGAAAGGTCTTACCCTCAGCTGGATCGGGGATGGGAACAATATC; CTGCACTCCATCATGATGAGCGCAGCGAAATTCGGAATGCACCTTCAGGCAGCTACTCCA; AAGGGTTATGAGCCGGATGCTAGTGTAACCAAGTTGGCAGAGCAGTATGCCAAAGAGAAT; GGTACCAAGCTGTTGCTGACAAATGATCCATTGGAAGCAGCGCATGGAGGCAATGTATTA; ATTACAGACACTTGGATAAGCATGGGACAAGAAGAGGAGAAGAAAAAGCGGCTCCAGGCT; TTCCAAGGTTACCAGGTTACAATGAAGACTGCTAAAGTTGCTGCCTCTGACTGGACATTT; TTACACTGCTTGCCCAGAAAGCCAGAAGAAGTGGATGATGAAGTCTTTTATTCTCCTCGA; TCACTAGTGTTCCCAGAGGCAGAAAACAGAAAGTGGACAATCATGGCTGTCATGGTGTCC; CTGCTGACAGATTACTCACCTCAGCTCCAGAAGCCTAAATTTTGA; ; Clone variation with respect to NM_000531.5;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000531 unedited; NAAGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGACTTT; GATAAGGAAGCTGAAGGGTGATATTACCTTTGCTCCCTCACTGCAACTGAACACATTTCT; TAGTTTTTAGGTGGCCCCCGCTGGCTAACTTGCTGTGGAGTTTTCAAGGGCATAGAATCG; TCCTTTACACAATTAAAAGAAGATGCTGTTTAATCTGAGGATCCTGTTAAACAATGCAGC; TTTTAGAAATGGTCACAACTTCATGGTTCGAAATTTTCGGTGTGGACAACCACTACAAAA; TAAAGTGCAGCTGAAGGGCCGTGACCTTCTCACTCTAAAAAACTTTACCGGAGAAGAAAT; TAAATATATGCTATGGCTATCAGCAGATCTGAAATTTAGGATAAAACAGAAAGGAGAGTA; TTTGCCTTTATTGCAAGGGAAGTCCTTAGGCATGATTTTTGAGAAAAGAAGTACTCGAAC; AAGATTGTCTACAGAAACAGGCTTTGCACTTCTGGGAGGACATCCTTGTTTTCTTACCAC; ACAAGATATTCATTTGGGTGTGAATGAAAGTCTCACGGACACGGCCCGTGTATTGTCTAG; CATGGCAGATGCAGTATTGGCTCGAGTGTATAAACAATCAGATTTGGACACCCTGGCTAA; AGAAGCATCCATCCCAATTATCAATGGGCTGTCAGATTTGTACCATCCTATCCAGATCCT; GGCTGATTACCTCACGCTCCAGGAACACTATAGCTCTCTGAAAGGTCTTACCCTCAGCTG; GATCGGGGATGGGAACAATATCCTGCACTCCATCATGATGAGCGCAGCGAAATTTCGAAT; GCACCTTNCAGCAGCTACTCCAAAGGGTTATGAGCCGATGCTAGTGTAACCAAGTTGGGC; AGGCAGTATGCCAAAGAGAATGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000531 unedited; NTTACTCTGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTAGG; TGACAGGATTTATTATGTACACCAAAGATGCAAGCGTCATTTCTAGAATTTTCGTTAAAC; TTCGGAGTTGTAATAGCTATATAGGAAAAGGGGTAAGAAGAAAGAGGAGATGAAAACAGC; TTTGCAAAAGTTAAGGGTGAATTTTCCTCCCCTATTTTCTTTTTTCCTGACAATATAAAT; AATGTCTGATTAAGATGAAAAGTTAAGAAAGCAAATACATTCCTACAGAATTGGGCTGAG; AATGAGGTTAAATTGTATGTACACAATAAAGAAAGCTATAGAGCTTTTTGAGGAAAAAAT; GACTATATTTTGGCAAGAAAATTATTACAAGTTCTTTCCAAGTGAGGGAAGAAGACTATA; CCATCCATTTAGTTCAAAGAATAACCAGGAATGAGATGGGAGCTCGTTACTATGGCAGAC; CATTTGGCAAGTGGAAGAACACTTCTTTCTATGTAGGCAATTTGAGGGTGAGCCTTCCAC; AATCCATATGACACCTTGTTCTACTCTCTGCTGAAAGATATTCCACTAAACTCCACTCTC; TTTCTAAGTGCTTCCAATAAATTACTATGGACACAAAAGTCACCTTTGACATTTACTGGA; AAATTTGATGTTTTTTTCTGTAACATTATGAGCCTCTTTCCCCCACAAAAAATGTTTTAT; TGCACCTTCATTGCAAATCTAATTTGTAATTCATCCCTTGCTTACTCATTTAAGTCAGAG; CTGTGTGCTGAAGAATATCAGTCAACCAGCCTGGAAACATTNTTCTAATAGTAAATGTAG; AATGGTAAGGAAATACATTATTNTGTCTTCTGTAAATGTATAATTTTTAAAAAACTTCAC; TATGTTATTTACATTNNTACTANNAATCAATATTCATATATTCACATACAATG;
• Restriction Sites: NotI-NotI
• ACCN: NM_000531
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000531.3, NP_000522.3
• RefSeq Size: 1927 bp
• RefSeq ORF: 1065 bp
• Locus ID: 5009
• UniProt ID: P00480
• Cytogenetics: Xp11.4
• Domains: OTCace, OTCace_N
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Summary: This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also. [provided by RefSeq, Jul 2008]