CD55 (NM_000574) Human Untagged Clone

CAT#: SC119802

CD55 (untagged)-Human CD55 molecule, decay accelerating factor for complement (Cromer blood group) (CD55), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD55
• Synonyms: CHAPLE; CR; CROM; DAF; TC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119802 sequence for NM_000574 edited (data generated by NextGen Sequencing)
  ATGACCGTCGCGCGGCCGAGCGTGCCCGCGGCGCTGCCCCTCCTCGGGGAGCTGCCCCGG
  CTGCTGCTGCTGGTGCTGTTGTGCCTGCCGGCCGTGTGGGGTGACTGTGGCCTTCCCCCA
  GATGTACCTAATGCCCAGCCAGCTTTGGAAGGCCGTACAAGTTTTCCCGAGGATACTGTA
  ATAACGTACAAATGTGAAGAAAGCTTTGTGAAAATTCCTGGCGAGAAGGACTCAGTGATC
  TGCCTTAAGGGCAGTCAATGGTCAGATATTGAAGAGTTCTGCAATCGTAGCTGCGAGGTG
  CCAACAAGGCTAAATTCTGCATCCCTCAAACAGCCTTATATCACTCAGAATTATTTTCCA
  GTCGGTACTGTTGTGGAATATGAGTGCCGTCCAGGTTACAGAAGAGAACCTTCTCTATCA
  CCAAAACTAACTTGCCTTCAGAATTTAAAATGGTCCACAGCAGTCGAATTTTGTAAAAAG
  AAATCATGCCCTAATCCGGGAGAAATACGAAATGGTCAGATTGATGTACCAGGTGGCATA
  TTATTTGGTGCAACCATCTCCTTCTCATGTAACACAGGGTACAAATTATTTGGCTCGACT
  TCTAGTTTTTGTCTTATTTCAGGCAGCTCTGTCCAGTGGAGTGACCCGTTGCCAGAGTGC
  AGAGAAATTTATTGTCCAGCACCACCACAAATTGACAATGGAATAATTCAAGGGGAACGT
  GACCATTATGGATATAGACAGTCTGTAACGTATGCATGTAATAAAGGATTCACCATGATT
  GGAGAGCACTCTATTTATTGTACTGTGAATAATGATGAAGGAGAGTGGAGTGGCCCACCA
  CCTGAATGCAGAGGAAAATCTCTAACTTCCAAGGTCCCACCAACAGTTCAGAAACCTACC
  ACAGTAAATGTTCCAACTACAGAAGTCTCACCAACTTCTCAGAAAACCACCACAAAAACC
  ACCACACCAAATGCTCAAGCAACACGGAGTACACCTGTTTCCAGGACAACCAAGCATTTT
  CATGAAACAACCCCAAATAAAGGAAGTGGAACCACTTCAGGTACTACCCGTCTTCTATCT
  GGGCACACGTGTTTCACGTTGACAGGTTTGCTTGGGACGCTAGTAACCATGGGCTTGCTG
  ACTTAG
  
  Clone variation with respect to NM_000574.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000574 unedited
  CGTCAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGGCGCGTC
  CTTGTTCTAACCCGGCGCGCCATGACCGTCGCGCGGCCGAGCGTGCCCGCGGCGCTGCCC
  CTCCTCGGGGAGCTGCCCCGGCTGCTGCTGCTGGTGCTGTTGTGCCTGCCGGCCGTGTGG
  GGTGACTGTGGCCTTCCCCCANTGTACCTAATGCCCAGCCAGCTTTGGAAGGCCGTACAA
  GTTTTCCCGAGGATACTGTAATAACGTACAAATGTGAAGAAAGCTTTGTGAAAATTCCTG
  GCGAGAAGGACTCAGTGATCTGCCTTAAGGGCAGTCAATGGTCAGATATTGAAGAGTTCT
  GCAATCGTAGCTGCGAGGTGCCAACAAGGCTAAATTCTGCATCCCTCAAACAGCCTTATA
  TCACTCAGAATTATTTTCCAGTCGGTACTGTTGTGGAATATGAGTGCCGTCCAGGTTACA
  GAAGAGAACCTTCTCTATCACCAAAACTAACTTGCCTTCAGAATTTAAAATGGTCCACAG
  CAGTCGAATTTTGTAAAAAGAAATCATGCCCCTATCCGGGAGANATACGAAATGGTCAGA
  TTGATGTACCAGGTGGCATATTATTTGGTGCCACCATCTCCTTCTCATGTAACACAGGNT
  ACAAATTTATTGGGCTCGACTTCTAGTTTTTGTCTTATTTCAGGCAGCTCTGTCCAGTGG
  AGTGACCCGTTTGCCAGAGTGCAGAGNAAATTTATTGTCCACCACACCCACAAATTGACC
  ATGGNATAATTCAGGGGGGACGTGACCATTATGGATATAGACAGTCTGTAACGTATTGCC
  ATGTATAAAGGATTCCCATTGATGGAAAGCCCTCCATTTAATTGACTGGGAATATGATGA
  AAGAAATTGGAATGGCCCACCACCTGAATGCCAAGGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000574 unedited
  CTCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTACCCAAAAAAGGGACCTTTTTTTTTTTTTTAAGAAAAA
  ACATTTTTTTTAACAAATTTTCTTAAACTTTTTTTGTTTTACATGAAAAACCAAAAGGGC
  CCCTGGCTTAAATAAATTTAAATAAATTTTCCAAAAAAATAAAATTTCCAATTTTGCCTT
  TGGCCCTCTCGATTAAAAAAAAAAAAAAAATCTTTTTTTATGTAAAAATGGGAATACCCC
  GGGATCCTTTTAAAAACTTTAAGCCAAAAAAAACCCCTTTCAAAGGCTTTTTCCAAACTT
  TTTTTGGGCAACAAAGGAAAATTCAGTCTTTCATTTTATTTTCCCCCCTTATAACCATAA
  ACCCCCCGGGTTCACCAACATGTTTTACCTTTAAAATTTTTGGCCACCCCGAAGGAAAAA
  TAAAAATGCCTTACCCCCAAAAATTCCTTTATAAGGGGGGCCATCTCTCTTTAACAATTT
  TGCTTAAAAAAAACTGAATGGGGTTTTTATTTGCTTTGGAAAACCAAAAAAGGGTTTTCA
  TTTTTTATTCCCTTTATAAGGGGAAATAACCTTCCAAATCTTGGGGAAAAAAAACCTTTC
  CTTTTTTTTTCCCGGGGACCCCTTTTTAAATAAAAACAAATTCCTATGCTTTTTTACCCT
  TTTAGGAAAGAGAACACCCTCATTTCTGCAAGTTGTTCTTTTTTCAAAAGGGGGAGGGGG
  GCTAAAAAAGGGATCCCAGAACTGCCTTTTTTCCCCTGCCCTGGGGAACTTCCCAACCAC
  ATCTTAAAGACATGAAACGCTTCCTAAAGAAAGAACCCAGTTGCTTTTTTTTTTCCAATT
  GCCAAAAAGTTTAAAAAACTGGAAACAGCCGTATCTCTGGTGTTTCTTTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000574
• Insert Size: 2310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000574.2, NP_000565.1
• RefSeq Size: 2308 bp
• RefSeq ORF: 1146 bp
• Locus ID: 1604
• UniProt ID: P08174
• Cytogenetics: 1q32.2
• Domains: CCP
• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades, Hematopoietic cell lineage, Viral myocarditis
• Gene Summary: This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (1) encodes isoform 1 (also known as gDAF), which is membrane-associated. Sequence Note: This RefSeq record represents the CD55*001.1.1 allele.