Muscarinic Acetylcholine Receptor M4 (CHRM4) (NM_000741) Human Untagged Clone

SKU SC119737

CHRM4 (untagged)-Human cholinergic receptor, muscarinic 4 (CHRM4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Muscarinic Acetylcholine Receptor M4
• Synonyms: HM4; M4R
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000741, RT-PCR generated; ATGGCCAACTTCACACCTGTCAATGGCAGCTCGGGCAATCAGTCCGTGCGCCTGGTCACG; TCATCATCCCACAATCGCTATGAGACGGTGGAAATGGTCTTCATTGCCACAGTGACAGGC; TCCCTGAGCCTGGTGACTGTCGTGGGCAACATCCTGGTGATGCTGTCCATCAAGGTCAAC; AGGCAGCTGCAGACAGTCAACAACTACTTCCTCTTCAGCCTGGCGTGTGCTGATCTCATC; ATAGGCGCCTTCTCCATGAACCTCTACACCGTGTACATCATCAAGGGCTACTGGCCCCTG; GGCGCCGTGGTCTGCGACCTGTGGCTGGCCCTGGACTACGTGGTGAGCAACGCCTCCGTC; ATGAACCTTCTCATCATCAGCTTTGACCGCTACTTCTGCGTCACCAAGCCTCTCACCTAC; CCTGCCCGGCGCACCACCAAGATGGCAGGCCTCATGATTGCTGCTGCCTGGGTACTGTCC; TTCGTGCTCTGGGCGCCTGCCATCTTGTTCTGGCAGTTTGTGGTGGGTAAGCGGACGGTG; CCCGACAACCAGTGCTTCATCCAGTTCCTGTCCAACCCAGCAGTGACCTTTGGCACAGCC; ATTGCTGCCTTCTACCTGCCTGTGGTCATCATGACGGTGCTGTACATCCACATCTCCCTG; GCCAGTCGCAGCCGAGTCCACAAGCACCGGCCCGAGGGCCCGAAGGAGAAGAAAGCCAAG; ACGCTGGCCTTCCTCAAGAGCCCACTAATGAAGCAGAGCGTCAAGAAGCCCCCGCCCGGG; GAGGCCGCCCGGGAGGAGCTGCGCAATGGCAAGCTGGAGGAGGCCCCCCCGCCAGCGTTG; CCACCGCCACCGCGCCCCGTGGCTGATAAGGACACTTCCAATGAGTCCAGCTCAGGCAGT; GCCACCCAGAACACCAAGGAACGCCCAGCCACAGAGCTGTCCACCACAGAGGCCACCACG; CCCGCCATGCCCGCCCCTCCCCTGCAGCCGCGGGCCCTCAACCCAGCCTCCAGATGGTCC; AAGATCCAGATTGTGACGAAGCAGACAGGCAATGAGTGTGTGACAGCCATTGAGATTGTG; CCTGCCACGCCGGCTGGCATGCGCCCTGCGGCCAACGTGGCCCGCAAGTTCGCCAGCATC; GCTCGCAACCAGGTGCGCAAGAAGCGGCAGATGGCGGCCCGGGAGCGCAAAGTGACACGA; ACGATCTTTGCCATTCTGCTAGCCTTCATCCTCACCTGGACGCCCTACAACGTCATGGTC; CTGGTGAACACCTTCTGCCAGAGCTGCATCCCTGACACGGTGTGGTCCATTGGCTACTGG; CTCTGCTACGTCAACAGCACCATCAACCCTGCCTGCTATGCTCTGTGCAACGCCACCTTT; AAAAAGACCTTCCGGCACCTGCTGCTGTGCCAGTATCGGAACATCGGCACTGCCAGGTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000741 unedited; NGGTAATTTTAGATTTGTAAACGACTCATATAGGCGGCCGCCATGTGATGTGAATCTGCA; GAATTCGGCTTCAACATGGCCAACTTCACACCTGTCAATGGCAGCTCGGGCAATCAGTCC; GTGCGCCTGGTCACGTCATCATCCCACAATCGCTATGAGACGGTGGAAATGGTCTTCATT; GCCACAGTGACAGGCTCCCTGAGCCTGGTGACTGTCGTGGGCAACATCCTGGTGATGCTG; TCCATCAAGGTCAACAGGCAGCTGCAGACAGTCAACAACTACTTCCTCTTCAGCCTGGCG; TGTGCTGATCTCATCATAGGCGCCTTCTCCATGAACCTCTACACCGTGTACATCATCAAG; GGCTACTGGCCCCTGGGCGCCGTGGTCTGCGACCTGTGGCTGGCCCTGGACTACGTGGTG; AGCAACGCCTCCGTCATGAACCTTCTCATCATCAGCTTTGGCCGCTACTTCTGCGTCACC; AAGCCTCTCACCTACCCTGCCCGGCGCACCACCAAGATGGCAGGCCTCATGATTGCTGCT; GCCTGGGTACTGTCCTTCGTGCTCTGGGCGCCTGCCATCTTGTTCTGGCAGTTTGTGGTG; GGTAAGCGGACGGTGCCCGACAACCAGTGCTTCATCCAGTTCCTGTCCAACCCAGCAGTG; ACCTTTGGCACGGCCATTGCTGCCTTCTACCTGCCTGTGGTCATCATGACGGTGCTGTAC; ATCCACATCTCCCTGGCCAGTCGCAGCCGAGTCCACAAGCACCGGCCCGAGGGCCCGANA; GAGAAGAAAGCCAAGACGCTGGCCTTCCTCAAGAGCCCCTAATGAAGCANAGCGTCAAGA; AGCCCCCGCCCCGGGGAGTCCNGCCTGGNNAGNAGCTGCGNCATGNNCAGCTNGNNAGAG; GCCCCCCCGNCAGCGCTGCCACCGCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000741 unedited; CTTTCCCNNAAATACTGTGNNACCGCGGCCGCTTACTAGATCGNAAGNCTTGNAATCGGT; ACCGAGCTCGGATCCACTATAACGGCCGCCATGTGCTGGAATTCGGCTTCCTGCCTGCCT; ACCTGGCAGTGCCGATGTTCCGATACTGGCACAGCAGCAGGTGCCGGAAGGTCTTTTTAA; AGGTGGCGTTGCACAGAGCATAGCAGGCAGGGTTGATAGTGCTGTTGACGTAGCAGAGCC; AGTAGCCAATGGACCACACCGTGTCAGGGATGCAGCTCTGGCAGAAGGTGTTCACCAGGA; CCATGACGTTGTAGGGCGTCCAGGTGAGGATGAAGGCTAGCAGAATGGCAAAGATCGTTC; GTGTCACTTTGCGCTCCCGGGCCGCCATCTGCCGCTTCTTGCGCACCTGGTTGCGAGCGA; TGCTGGCGAACTTGCGGGCCACGTTGGCCGCAGGGCGCATGCCAGCCGGCGTGGCAGGCA; CAATCTCAATGGCTGTCACACACTCATTGCCTGTCTGCTTCGTCACAATCTGGATCTTGG; ACCATCTGGAGGCTGGGTTGAGGGCCCGCGGCTGCAGGGGAGGGGCGGGCATGGCGGGCG; TGGTGGCCTCTGTGGTGGACAGCTCTGTGGCTGGGCGTTCCTTGGTGTTCTGGGTGGCAC; TGCCTGAGCTGGACTCATTGGAAGTGTCCTTATCANCCACGGGGCGCGGTGGCGGTGGCA; GCGCTGGCGGGGGGGCCTCCTCCAGCTTGCCATTGCCCAACTCCTCCCGGGCGGCCTCCC; GGGCGGGGGCCTCTTTGACCCCTTGTTTCATTAATGGGCTCTTTGAGAAGGCCACGGTTT; TGGGTTTTTCTTCCTTCCGGCCCTCGGCCGGTGCTTGGGGACTCG;
• Restriction Sites: Please inquire
• ACCN: NM_000741
• Insert Size: 1440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000741.2, NP_000732.2
• RefSeq Size: 1468 bp
• RefSeq ORF: 1440 bp
• Locus ID: 1132
• UniProt ID: P08173
• Cytogenetics: 11p11.2
• Protein Families: Druggable Genome
• Protein Pathways: Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton
• Summary: The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. [provided by RefSeq, Jul 2008]