CD3D (NM_000732) Human Untagged Clone

CAT#: SC119732

CD3D (untagged)-Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD3D
• Synonyms: CD3-DELTA; IMD19; T3D
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119732 sequence for NM_000732 edited (data generated by NextGen Sequencing)
  ATGGAACATAGCACGTTTCTCTCTGGCCTGGTACTGGCTACCCTTCTCTCGCAAGTGAGC
  CCCTTCAAGATACCTATAGAGGAACTTGAGGACAGAGTGTTTGTGAATTGCAATACCAGC
  ATCACATGGGTAGAGGGAACGGTGGGAACACTGCTCTCAGACATTACAAGACTGGACCTG
  GGAAAACGCATCCTGGACCCACGAGGAATATATAGGTGTAATGGGACAGATATATACAAG
  GACAAAGAATCTACCGTGCAAGTTCATTATCGAATGTGCCAGAGCTGTGTGGAGCTGGAT
  CCAGCCACCGTGGCTGGCATCATTGTCACTGATGTCATTGCCACTCTGCTCCTTGCTTTG
  GGAGTCTTCTGCTTTGCTGGACATGAGACTGGAAGGCTGTCTGGGGCTGCCGACACACAA
  GCTCTGTTGAGGAATGACCAGGTCTATCAGCCCCTCCGAGATCGAGATGATGCTCAGTAC
  AGCCACCTTGGAGGAAACTGGGCTCGGAACAAGTGA
  
  Clone variation with respect to NM_000732.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000732 unedited
  NGGGGCGTTCCAAATATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGNAC
  TAGGGGAGGGCAGCTCTCACCCAGGCTGATAGTTCGGTGACCTGGCTTTATCTACTGGAT
  GAGTTCCGCTGGGAGATGGAACATAGCACGTTTCTCTCTGGCCTGGTACTGGCTACCCTT
  CTCTCGCAAGTGAGCCCCTTCAAGATACCTATAGAGGAACTTGAGGACAGAGTGTTTGTG
  AATTGCAATACCAGCATCACATGGGTAGAGGGAACGGTGGGAACACTGCTCTCAGACATT
  ACAAGACTGGACCTGGGAAAACGCATCCTGGACCCACGAGGAATATATAGGTGTAATGGG
  ACAGATATATACAAGGACAAAGAATCTACCGTGCAAGTTCATTATCGAATGTGCCAGAGC
  TGTGTGGAGCTGGATCCAGCCACCGTGGCTGGCATCATTGTCACTGATGTCATTGCCACT
  CTGCTCCTTGCTTTGGGAGTCTTCTGCTTTGCTGGACATGAGACTGGAAGGCTGTCTGGG
  GCTGCCGACACACAAGCTCTGTTGAGGAATGACCAGGTCTATCAGCCCCTCCGAGATCGA
  GATGATGCTCAGTACAGCCACCTTGGAGGAAACTGGGCTCGGAACAAGTGAACCTGAGAC
  TGGTGGCTTCTAGAAGCAGCCATTACCAACTGTACCTTCCCTTCTTGCTCAGCCAATAAA
  TATATCCTCTTTCACTCAGAAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGG
  CCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAG
  TGCCTCTCCTGGCCCTGGNGAGTGCACTCCAGTGCCCACCAGCCTGTCCTAATNAATAAG
  T
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000732 unedited
  NGGTACATCTATGNACCGCGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTT
  TCTGAGTGAAAGAGGATATATTTATTGGCTGAGCAAGAAGGGAAGGTACAGTTGGTAATG
  GCTGCTTCTAGAAGCCACCAGTCTCAGGTTCACTTGTTCCGAGCCCAGTTTCCTCCAAGG
  TGGCTGTACTGAGCATCATCTCGATCTCGGAGGGGCTGATAGACCTGGTCATTCCTCAAC
  AGAGCTTGTGTGTCGGCAGCCCCAGACAGCCTTCCAGTCTCATGTCCAGCAAAGCAGAAG
  ACTCCCAAAGCAAGGAGCAGAGTGGCAATGACATCAGTGACAATGATGCCAGCCACGGTG
  GCTGGATCCAGCTCCACACAGCTCTGGCACATTCGATAATGAACTTGCACGGTAGATTCT
  TTGTCCTTGTATATATCTGTCCCATTACACCTATATATTCCTCGTGGGTCCAGGATGCGT
  TTTCCCAGGTCCAGTCTTGTAATGTCTGAGAGCAGTGTTCCCACCGTTCCCTCTACCCAT
  GTGATGCTGGTATTGCAATTCACAAACACTCTGTCCTCAAGTTCCTCTATAGGTATCTTG
  GAGGGGCTCACTTGCGAGAGAAAGGTACCCAGTCCCAGGCCAGAGAAAACGTGCTATGTT
  CCATCTCCCAACGGAACTCATCCAGTAGATAAAGCCAGGTCCCCGACTATCAGCCTGGGG
  GAGAACTGTCCTCCCCTAACTCTGGTGCCGAATTCTCGGCCCGCCCTATGTGGGGGCGTA
  TTACAAAATTTTGAGGGGTTACTAAACGAACTTTGTTTTATAAAACCTCCCACCTACACC
  CCTCCCGCCCTTTTTGTCACCGGGGGGGGGTTTTACCACATTTTGGAAAGCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000732
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000732.3, NP_000723.1
• RefSeq Size: 1585 bp
• RefSeq ORF: 516 bp
• Locus ID: 915
• UniProt ID: P04234
• Cytogenetics: 11q23.3
• Domains: ITAM
• Protein Families: Druggable Genome
• Protein Pathways: Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway
• Gene Summary: The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (A).