GABA A Receptor beta 2 (GABRB2) (NM_000813) Human Untagged Clone

SKU SC119629

GABRB2 (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, beta 2 (GABRB2), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: GABA A Receptor beta 2
• Synonyms: DEE92; ICEE2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC119629 representing NM_000813.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCGGCCGCGAATTCGGCACGAGGACTAAGTTGGATCTCCTCTCTTCAGTGAAACCCTCAATTCCATCAA; AAACTAAAGGGATGTGGAGAGTGCGGAAAAGGGGCTACTTTGGGATTTGGTCCTTCCCCTTAATAATCG; CCGCTGTCTGTGCGCAGAGTGTCAATGACCCTAGTAATATGTCGCTGGTTAAAGAGACGGTGGATAGAC; TCCTGAAAGGCTATGACATTCGTCTGAGACCAGATTTTGGAGGTCCCCCCGTGGCTGTGGGGATGAACA; TTGACATTGCCAGCATCGATATGGTTTCTGAAGTCAATATGGATTATACCTTGACAATGTACTTTCAAC; AAGCCTGGAGAGATAAGAGGCTGTCCTATAATGTAATACCTTTAAACTTGACTCTGGACAACAGAGTGG; CAGACCAGCTCTGGGTGCCTGATACCTATTTCCTGAACGATAAGAAGTCATTTGTGCACGGAGTGACTG; TTAAGAACCGCATGATTCGCCTGCATCCTGATGGCACCGTCCTTTATGGACTCAGAATCACAACCACAG; CTGCCTGCATGATGGACCTAAGGAGGTACCCACTGGATGAACAAAACTGCACCTTGGAAATTGAGAGCT; ATGGATACACAACTGATGACATTGAGTTTTACTGGCGTGGCGATGATAATGCAGTAACAGGAGTAACGA; AAATTGAACTTCCACAGTTCTCTATTGTAGATTACAAACTTATCACCAAGAAGGTTGTTTTTTCCACAG; GTTCCTATCCCAGGTTATCCCTCAGCTTTAAGCTTAAGAGAAACATTGGCTACTTTATCCTGCAAACAT; ACATGCCTTCCATCCTGATTACCATCCTCTCCTGGGTCTCCTTCTGGATTAATTACGATGCTTCAGCTG; CAAGGGTGGCATTAGGAATCACAACTGTCCTCACAATGACCACAATCAACACCCACCTCCGGGAAACTC; TCCCTAAAATCCCCTATGTGAAGGCCATTGACATGTACCTGATGGGGTGCTTTGTCTTCGTTTTCATGG; CCCTTCTGGAATATGCCCTAGTCAACTACATCTTCTTTGGGAGGGGGCCCCAACGCCAAAAGAAAGCAG; CTGAGAAGGCTGCCAGTGCCAACAATGAGAAGATGCGCCTGGATGTCAACAAGATGGACCCCCATGAGA; ACATCTTACTGAGCACTCTCGAGATAAAAAATGAAATGGCCACATCTGAGGCTGTGATGGGACTTGGAG; ACCCCAGAAGCACAATGCTAGCCTATGATGCCTCCAGCATCCAGTATCGGAAAGCTGGGTTGCCCAGGC; ATAGTTTTGGCCGAAATGCTCTGGAACGACATGTGGCGCAAAAGAAAAGTCGCCTGAGGAGACGCGCCT; CCCAACTGAAAATCACCATCCCTGACTTGACTGATGTGAATGCCATAGATCGGTGGTCCCGCATATTCT; TCCCAGTGGTTTTTTCCTTCTTCAACATCGTCTATTGGCTTTATTATGTGAACTAAAACATGGCCTCCC; ACTGGAAGCAAGGACTAGATTCCTCCTCAAACCAGTTGTACAGCCTGATGTAGGACTTGGAAAACACAT; CAATCCAGGACAAAAGTGACGCTAAAATACCTTAGTTGCTGGCCTATCCTGTGGTCCATTTCATACCAT; TTGGGTTGCTTCTGCTAAGTAATGAATACACTAAGGTCCTTGTGGTTTTCCAGTTAAAACGCAAGTGAT; TTTTACACATGGTGGCTAGACAGCCTGAGTGCTCCATGTTGTCTGTTTAGTATGCAGCCTACTCAGAGG; GGAATAATTTGGAAGATATTCTTAGAAGGCTCAACAGCATTGTCAGTCATTTGGTCATAACCAGATACT; CCTCATCTTCCTTTTTAAGGTCGGCATGCTGTTGAGATGGCATTGTCCTTATGAAACATTATCTGTAAT; GCCATGCAAACACATTACTTGAAGATGATCTATGATTTTCTTACTGAGATGATAATGGTGTCATGGGAA; GATTATTCCCACTGGTTTCTAAAAGATTCTCTTGTGATTTGAGCAGGCTAGGGGAATCAAGACAGAGAC; TCAGGAAACCGATTTGGCCAGTCCCTTTGCTTGAGCACGGAGAATCAACTATGATGTCTTTCATTTTGG; CATTTAGAGATGAGGAGACTTCTGGACAATATCATGCATGGTGGCCAAGAAAATTTTTGCTAAACGGCT; ATTTCCTAGGTGGTTAAAGTAAGAGTTAACAACACAGAGCAGAGTAGAAAATCAGAAACGACGGTTGTA; ACAAAAAGCAAGCAAGCAAGAAAGCATGACCCTTATCAGCTCCACACAGCATCTGGTCTATACATACTG; TGTCTAGTGGCAGAGGGTGAGTAAGAGAGATTGTTTTTCCAACATCACTAGCTTTTGACAGCATACCTA; AAGAGGTTTTTATAGTTTAATCGAAGATAACCATGAAATACAAAAATTAGAGAAATGAGAATTGTCTTA; GATATTCAGGTTAGATTCAGAGTCATTCAAATATACAAATACAGAGCACAAACTAGCAAACAGATTGTT; TTTCTCAGACGAAGTCAGCTGTCCCTGCAATTTTTGTCCCTATAATTTTATTTCTTAATTTAATAGAGT; ATTTCAAGAGAAGGATTTGTTCATCACTTTAAAGGATTCATGGTCCTCTCCAGCTTTTTTTTTGCTTCC; AAAATTCTGATTATATTCTTAGGAGACTGGCAAAGTAAGGCTTTGGAGAGATTGAGTGAACTATATTTT; CTTATATGCAATGAAATTCACCATTGTATTTTTCAAAGCAAATAATACTTCAAGGAGTATGAACACACA; GAGAGTTAATTTGTGCATTCATTGGTTTGGGGCTTGATTTTCTTAAGAGTGCTTTCATTGTATATGTTG; CAATCTGCCTAATCAAGGAAAATATGAAAACTAGTGGAAAATAATAACAAAGCCACATTAACATGATAA; ATGTTATGAGCCTCAGTTGATATTGCAGAAAAATATATAACTGTTAATCAGGCAGGCATGATGTATTTT; TTTTCTATTTTTAAGTTATGCCATTGTGCTTCTTTCTACACTAATTGGACATAAGCATTCATATGTTGG; ACAAGAGGAGAATAAGCTAATATGTCTTCCTTCTATCAATATAAGGCTCATATTTTGCTTTTCTTATAG; AAAATAGTGAATTGCTAAATTTTCCTTAAAGTTGAATTTGATTGAACTTTTTCTTGGAGGATGACTTTG; CTATTTCTATTTTGAAAACAAAGAAAGCTCTTCTTTTTAGAGTAATATCCCTAATTGTGCATAGCAGCC; AGTATCCACCTGTGAATAATGTATGTTTTCTTGATTCCTTGTTTCCTTCATGATTTAGCAGCTTGGAAT; ATGATGGAGTACTATTGCCCTTGCATTCCAGCTGTCCAAGAACAACAACAAACCTTTATCCTTCAGCTT; TCTGAATTTGCCCCAAAATGGAATGGTATTTTGTTTTTCACCAGTGTAGGAAACCTTGGCCATTATATT; TTGATTACATAATCTTGAGCACCTCTGAGTATCATCTTTGGGCAGAATCTGACCAAGATTCTTCTCACA; GGCTCAGAGTAGAGATTCCTCATGTTTCTCCAGGACACACTTTCTCCTAGGCAATCATTTTGGGGCATC; ACACTAAGTAAAGTGCCTGCCCCTTGTCAACAGGGGGTAAAGTTTGACTAAAAAACATCAGGAAGGAAC; ATATTGATAACTCAGGGGCACAACTCCAAAATGCCATTCTGAAAAGGCTCTTCTAGAGTCAACTCAGAG; CCCAAGTGTAGAAGTGGGCTCTTATGTGCAATCACTGTACAAGCCATAAAAATCTTTGATATTGCCATT; TATGATTACAACAAAATTTTAGATGTCCAAGAAAGTCAACAGAACAATCCAGATCAAAGTGTAGAGAAG; CATCTTTGCAGCTTGAGCCTCAAAAGCATAAAACCAGGAATTCATTTCTGCTCTCAGTGATACAAGAAA; CAAGGCAAAGGAAAAAAGATGGGACCTTAGAAGCAATGCCCTAGAGTAAAAAGCATACATTGGCACTCA; GTAACCACAGAGATAGAAACAACTGACTCAGCCTCTCCAGAAATAACAGTAGATGTAGAATTTGTTCTT; AAATATATGTTTCGTTGAAATGAAATGCAGTCATCAAATGCGAGGAGGGGTGCTAGACCTGATAAGTCT; AGGAAGACCAGTCAGCTTGATAGCCAGTTGTTAAACTAAAGGACCACAAAGGTGTGTTTAGTAGTTCTG; ATATGGGATTACTATCAGTTTATACATATGTATAGATTTTCTTTTAATTATAAACAGCCAGATAGCCAG; CAATTAAGGTGTTTTTTCATCTTTGTGAAATTCATGGACAAATTCTGACTCTAAAATGCACAGTCTACA; TGAAATCTTTGATCTCAATCTGTATATATATATATTGTACATGACTGCTAGTAGGTATGAAATGCATTT; CAGAGTTGAAAACTCATGCAACATAATCATGTGTTTGCATAAGGAATGTTAAAATTCTTTCTACTACAA; TTTATTAACAGGGGAAAAGATTCAAGCAGTGCCATCTAGACCTTTTCTCTTCTCATTTCTTCAAAAGCA; GTGCTAAGTAAAATAATAATAATAAAAATGGTTTATGAACCAAAAGACTTTACATTCTAGCATTAGGAA; CACACACAAAAAAATCTGTCAGCTTACAAAAGCACAACACATAAAGAAAGAGCAGTGGAATGCGTAATG; GGGAAATGAATCATGTTTAAAGCAAGACTTGCATTAAATTGGCAACTAAGATAATTTCCATTTTCTATA; GGTAACCGTGTTCCAGTTTAATGATTACCTTCATACTCAACAAGAAAAATGGCAAAGCAACATCATCAA; AATAGGTCTTACAACATATCTACATGTATATATGTGTATATATAGATTCATATAAACATACATACATAT; ATATGCATTCATATGTTGGAAAACAAAAGGAATAAGCTAATATGTTTTATTTCCTTCATTAAACCAGTA; TATGAGTATTTTTAATTTGGCAAACAAAAATCAACATACTGAAATAAGTAATACAGGGTACTGAGCATT; TCTCTATCAGCAAATCAATGCCTACAGTTTTTATGAACCATTGCACAGTTCAACTCGACAATAGTACTG; TACCCCAGAACATCAGAAGTTAAGTAGTTGTCACATTGCTTGTCTAATTGCTCTCTTTTTCAAGGAAAC; AAATAACATTGTCCATTGGTGAGTCAAAAACAAACATGTCTTAAGAAAATGAAAAATGATAAAAATTCC; TCCAAAGAGGAAGAAAAAAAAAAACTAAACCCCACCATGTTGGTTAGGGCAAAATACTAAGTATTCACA; ACTGTAGCAATCATGCCATTTGGCTAAATGTACAGAGTACATGTAATGTGAGCAACCATAACTTTCACT; GAACGTGTGTAAATATTAAAACAGATTTCTTAAATATTTTTAACTTCTAATTTGTATTTTATGGTAAGG; CAATGTGCTAATTCCGTTTATGGCTTAAGTTGATGTGTTATAATATGTAAATAATTAAACATGCTGTAA; ATGAATAGTCAATAGATACAAATAATTGTTTAGTTTTTTTCTTAATTTTTATTTATTTTTTACTAAGTA; CTATTACTGCAGTGCCCCCAGCAGAGTAATTTAAACACCCTTTTGCATAAATGGTTTGGGAATCCCAGA; CAGAAGGGTTTCAATTTGCTATCACACACACAGTCACACACAGTCACACACACACACACACACACTCAA; ACACACATAATCTTTTGCTCACCCACTCTGTCTCTCTGCCCGTCTATGGGAAACACTCTACTATTTATG; TAGTCAATATAACAAACAAGAGATAGCCTTTGAAATGGCAATTGGGAAAAGCAAAAATGATCATTGATA; ACTTAATAGACTTTTTTATATGTCAGAGTTCAAGAAACCAAAGTTTGCAACTTAAATCTCAGTTTTCAA; ACAGTAAAAGTTTGTATAGATGGCACTACAGAAAAATAAAAATGGTTATTCCTACCAGCTCCAGAAATG; TGTTACACAATATACACTAGAAGACTTTTTTTTTAATAATAGTATTTTGTCCTTAGGTAGTATTGTTCC; TTTCAACCAAAGACTCCAGAATTATTGAAAAACAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGC; CGC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000813 unedited; CCAATTTTGTAATACGACTCACTATAGGCGGCCGCGACATTCGCACGAGGACTAAGTTGG; ATCTCCTCTCTTCAGTGAAACCCTCAATTCCATCAAAAACTAAAGGGATGTGGAGAGTGC; GGAAAAGGGGCTACTTTGGGATTTGGTCCTTCCCCTTAATAATCGCCGCTGTCTGTGCGC; AGAGTGTCAATGACCCTAGTAATATGTCGCTGGTTAAAGAGACGGTGGATAGACTCCTGA; AAGGCTATGACATTCGTCTGAGACCAGATTTTGGAGGTCCCCCCGTGGCTGTGGGGATGA; ACATTGACATTGCCAGCATCGATATGGTTTCTGAAGTCAATATGGATTATACCTTGACAA; TGTACTTTCAACAAGCCTGGAGAGATAAGAGGCTGTCCTATAATGTAATACCTTTAAACT; TGACTCTGGACAACAGAGTGGCAGACCAGCTCTGGGTGCCTGATACCTATTTCCTGAACG; ATAAGAAGTCATTTGTGCACGGAGTGACTGTTAAGAACCGCATGATTCGCCTGCATCCTG; ATGGCACCGTCCTTTATGGACTCAGAATCACAACCACAGCTGCCTGCATGATGGACCTAA; GGAGGTACCCACTGGATGAACAAAACTGCACCTTGGAAATTGAGAGCTATGGATACACAA; CTGATGACATTGAGTTTTACTGGCGTGGCGATGATAATGCAGTAACAGGAGTAACGAAAA; TTGAACTTCCACAGTTCTCTATTGTAGATTACAAACTTATCACCAAGAAGGTTGTTTTTT; CCACAGGTTCCTATCCCAGGTTATCCCTCAGCTTTAAGCTAAGAGAACATTGGCTACTNT; ATCCTGCANCATACATGCCTTCCATCCTGATACCATCCTCTCCTGGGTCTCCTTCTGGAT; TAATACGATGCTTCAGCTGCAGGTGGCATTAGAATACACTGTCTCACC;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_000813 unedited; NAAATTTTGGGATGCCCTCTTAATACTCTTCTTTGGGAGGGGCCCCAACGCCAAAAAAAG; CACTGAGAAGGCTGCCAGTGCCAACAATGAGAAGATGCGCCTGGATGTCAACAAGATGGA; CCCCCATGAGAACATCTTACTGAGCACTCTCGAGATAAAAAATGAAATGGCCACATCTGA; GGCTGTGATGGGACTTGGATACCCCAGAAGCACAATGCTAGCCTATGATGCCTCCAGCAT; CCAGTATCGGAAAGCTGGGTTGCCCAGGCATAGTTTTGGCCGAAATGCTCTGGAACGACA; TGTGGCGCANAAGAAAAGTCGCCTGAGGAGACGCGCCTCCCAACTGANAATCACCATCCC; TGACTTGACTGATGTGAATGCCATAGATCGGTGGTCCCGCATATTCTTCCCAGTGGTTTT; TTCCTTCTTCAACATCGTCTATTGGCTTTATTATGTGAACTAAAACATGGCCTCCCACTG; GAAGCAAGGACTANGATTCCTCCTCAACCAGTTGTACAGCCTGATGTANGACTTNGNAAA; CACANTCAATCCAGACAAAAGTGANCGCTAAATACCTTAGTTGCTGGCCTATCCTGTGGT; CCATTTCATACCATTTGGGTTGCTTCTGCTAAGTAATGAATACACTAAAGTCCTTGTGGT; TTTCCAGTTAAAACGCAAGTGATTTTTACACATGGTGGCTAGACAGCCTGAGTGCTNCAT; GGTGGCTGTTTAGTATGCAGCCTACTCAGAGGGGAATAATTTTGGAAGATAATCTTAGGA; GGCTCAACAGCATTTGTCAGTCATTTTGGGTCATACCAGATACTCCTCATCTTCCTTTTT; TAAGGTCGGCATGCTGTTGAAATGGCATTGTCCCTTATGAACATTATCTGTNATGCCATG; CN;
• Restriction Sites: NotI-NotI
• ACCN: NM_000813
• Insert Size: 6213 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000813.1
• RefSeq Size: 1860 bp
• RefSeq ORF: 6213 bp
• Locus ID: 2561
• UniProt ID: P47870
• Cytogenetics: 5q34
• Domains: Neur_chan_LBD, Neur_chan_memb
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 239.4 kDa
• Summary: The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes GABA A receptor, beta 2 subunit. It is mapped to chromosome 5q34 in a cluster comprised of genes encoding alpha 1 and gamma 2 subunits of the GABA A receptor. Alternative splicing of this gene generates 2 transcript variants, differing by a 114 bp insertion. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) lacks 114 bp within the coding region, as compared to variant 1, resulting in an isoform (2) which is 38 aa shorter.