NQO1 (NM_000903) Human Untagged Clone

CAT#: SC119599

NQO1 (untagged)-Human NAD(P)H dehydrogenase, quinone 1 (NQO1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NQO1
• Synonyms: DHQU; DIA4; DTD; NMOR1; NMORI; QR1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000903 edited
  GAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGTTGCAGCCGGAGCCGCCCAGC
  TCACCGAGAGCCTAGTTCCGGCCAGGGTCGCCCCGGCAACCACGAGCCCAGCCAATCAGC
  GCCCCGGACTGCACCAGAGCCATGGTCGGCAGAAGAGCACTGATCGTACTGGCTCACTCA
  GAGAGGACGTCCTTCAACTATGCCATGAAGGAGGCTGCTGCAGCGGCTTTGAAGAAGAAA
  GGATGGGAGGTGGTGGAGTCGGACCTCTATGCCATGAACTTCAATCCCATCATTTCCAGA
  AAGGACATCACAGGTAAACTGAAGGACCCTGCGAACTTTCAGTATCCTGCCGAGTCTGTT
  CTGGCTTATAAAGAAGGCCATCTGAGCCCAGATATTGTGGCTGAACAAAAGAAGCTGGAA
  GCCGCAGACCTTGTGATATTCCAGTTCCCCCTGCAGTGGTTTGGAGTCCCTGCCATTCTG
  AAAGGCTGGTTTGAGCGAGTGTTCATAGGAGAGTTTGCTTACACTTACGCTGCCATGTAT
  GACAAAGGACCCTTCCGGAGTAAGAAGGCAGTGCTTTCCATCACCACTGGTGGCAGTGGC
  TCCATGTACTCTCTGCAAGGGATCCACGGGGACATGAATGTCATTCTCTGGCCAATTCAG
  AGTGGCATTCTGCATTTCTGTGGCTTCCAAGTCTTAGAACCTCAACTGACATATAGCATT
  GGGCACACTCCAGCAGACGCCCGAATTCAAATCCTGGAAGGATGGAAGAAACGCCTGGAG
  AATATTTGGGATGAGACACCACTGTATTTTGCTCCAAGCAGCCTCTTTGACCTAAACTTC
  CAGGCAGGATTCTTAATGAAAAAAGAGGTACAGGATGAGGAGAAAAACAAGAAATTTGGC
  CTTTCTGTGGGCCATCACTTGGGCAAGTCCATCCCAACTGACAACCAGATCAAAGCTAGA
  AAATGAGATTCCTTAGCCTGGATTTCCTTCTAACATGTTATCAAATCTGGGTATCTTTCC
  AGGCTTCCCTGACTTGCTTTAGTTTTTAAGATTTGTGTTTTTCTTTTTCCACAAGGAATA
  AATGAGAGGGAATCGACTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  ACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000903 unedited
  ATCGGGATTTGTAACCGACTTATATAGGCGGCCGCGCAATTCGCACGAGGCCTCGTGCCG
  AATTCGGCACGAGGTTGCAGCCGGAGCCGCCCAGCTCACCGAGAGCCTAGTTCCGGCCAG
  GGTCGCCCCGGCAACCACGAGCCCAGCCAATCAGCGCCCCGGACTGCACCAGAGCCATGG
  TCGGCAGAAGAGCACTGATCGTACTGGCTCATTCAGAGAGGACGTCCTTCAACTATGCCA
  TGAAGGAGGCTGCTGCAGCGGCTTTGAAGAAGAAAGGATGGGAGGTGGTGGAGTCGGACC
  TCTATGCCATGAACTTCAATCCCATCATTTCCAGAAAGGACATCACAGGTAAACTGAAGG
  ACCCTGCGAACTTTCAGTATCCTGCCGAGTCTGTTCTGGCTTATAAAGAAGGCCATCTGA
  GCCCAGATATTGTGGCTGAACAAAAGAAGCTGGAAGCCGCAGACCTTGTGATATTCCAGT
  TCCCCCTGCAGTGGTTTGGAGTCCCTGCCATTCTGAAAGGCTGGTTTGAGCGAGTGTTCA
  TAGGAGAGTTTGCTTACACTTACGCTGCCATGTATGACAAAGGACCCTTCCGGAGTAAGA
  AGGCAGTGCTTTCCATCACCACTGGTGGCAGTGGCTCCATGTACTCTCTGCAAGGGATCC
  ACGGNGACATGAATGTCATTCTCTGGCCAATTCAGAGTGGCATTCTGCATTTCTGTGGCT
  TCCAAGTCTTAGAACCTCAACTGACATATAGCATTGGGCACACTCCAGCAGACGCCCGAA
  TTCAAATCCTGGNAGGGATGGAAGAACGCCTGGAGAATATTTGGGATGAGACACCACTGT
  ATTTTGCTCCNAGCAGCCTCTTTGACCTANACTTCCCAGNCAGGATTCTTAATGAAAAAA
  GAAGTACAGGATGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000903 unedited
  GGCCGCAATCTAGAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TAACAGGCGATTCCCTCTCATTTATTCCTTGGGGAAAAAAAAAAACACAAATCTTAAAAA
  CTAAAGCAAGTCAGGGAACCCTGGAAAGATACCCAAATTTGATAACATGTTAAAAGGAAA
  TCCAGGCTAAGGAATCTCATTTTCTAGCTTTGATCTGGTTGTCAGTTGGGATGGACTTGC
  CCAAGTGATGGCCCACAAAAAGGCCAAATTTCTTGTTTTTCTCCTCATCCTGTACCTTTT
  TTTTCATTAAAAATCCTGCCTGGAAGTTTAGGTCAAAAAGGCTGCTTGGAGCAAAATACA
  GGGGGGTCTCATCCCAAATATTCTCCAGGCGTTTCTTCCATCCTTCCAGGATTTGAATTC
  GGGCGTCTGCTGGAGTGTGCCCAATGCTATATGTCAGTGGAGGTTCTAAAACTTGGAAGC
  CACAGAAATGCAAAATGCCACTCTGAATTGGCCAAAAAATGACATTCATGTCCCCGTGGA
  TCCCTTGCAAAGAGTACATGGAGCCACTGCCACCAGTGGTGATGGAAAGCACTGCCTTCT
  TACTCCGGAAGGGTCCTTTGTCATACATGGCAGCGTAAGTGTAAGCAAACTCTCCTATGA
  ACACTCGCTCAAACCAGCCTTTCAGATGGCAGGGACTCCAAACCACTGCAGGGGGAACTG
  GAATTCACAAGGTCTGCGGCTTACAGCTTCTTTTGTTCACCCACAATATCTGGGCTCANA
  TGGCCTTCTTTTAAGCCAAACAGACTCGGCAGATACTTGAAGTCGCAGGGCCCTTAGTTA
  CCTGGATGNCCTTCCTGAAAGATGGGATTGAGTTATGCCTANAGGCCGACTCCCACCTTC
  ATCTTTTTTTAAAGCGCTGAGACCCTCTATGATTAATGAAGACGTCTTTTAGGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000903
• Insert Size: 1120 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000903.2, NP_000894.1
• RefSeq Size: 2601 bp
• RefSeq ORF: 825 bp
• Locus ID: 1728
• UniProt ID: P15559
• Cytogenetics: 16q22.1
• Domains: Flavodoxin_2
• Protein Families: Druggable Genome
• Gene Summary: This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).