RPL12 (NM_000976) Human Untagged Clone

SKU SC119578

RPL12 (untagged)-Human ribosomal protein L12 (RPL12)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RPL12
• Synonyms: L12
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119578 sequence for NM_000976 edited (data generated by NextGen Sequencing); ATGCCGCCGAAGTTCGACCCCAACGAGATCAAAGTCGTATACCTGAGGTGCACCGGAGGT; GAAGTCGGTGCCACTTCTGCCCTGGCCCCCAAGATCGGCCCCCTGGGTCTGTCTCCAAAA; AAAGTTGGTGATGACATTGCCAAGGCAACGGGTGACTGGAAGGGCCTGAGGATTACAGTG; AAACTGACCATTCAGAACAGACAGGCCCAGATTGAGGTGGTGCCTTCTGCCTCTGCCCTG; ATCATCAAAGCCCTCAAGGAACCACCAAGAGACAGAAAGAAACAGAAAAACATTAAACAC; AGTGGGAATATCACTTTTGATGAGATTGTCAACATTGCTCGACAGATGCGGCACCGATCC; TTAGCCAGAGAACTCTCTGGAACCATTAAAGAGATCCTGGGGACTGCCCAGTCAGTGGGC; TGTAATGTTGATGGCCGCCATCCTCATGACATCATCGATGACATCAACAGTGGTGCTGTG; GAATGCCCAGCCAGTTAA; ; Clone variation with respect to NM_000976.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000976 unedited; CACGAGGCCAAGGTGCAACTTCCTTCGGTCGTCCCGAATCCGGGTTCATCCGACACCAGC; CGCCTCCACCATGCCGCCGAAGTTCGACCCCAACGAGATCAAAGTCGTATACCTGAGGTG; CACCGGAGGTGAAGTCGGTGCCACTTCTGCCCTGGCCCCCAAGATCGGCCCCCTGGGTCT; GTCTCCAAAAAAAGTTGGTGATGACATTGCCAAGGCAACGGGTGACTGGAAGGGCCTGAG; GATTACAGTGAAACTGACCATTCAGAACAGACAGGCCCAGATTGAGGTGGTGCCTTCTGC; CTCTGCCCTGATCATCAAAGCCCTCAAGGAACCACCAAGAGACAGAAAGAAACAGAAAAA; CATTAAACACAGTGGGAATATCACTTTTGATGAGATTGTCAACATTGCTCGACAGATGCG; GCACCGATCCTTAGCCAGAGAACTCTCTGGAACCATTAAAGAGATCCTGGGGACTGCCCA; GTCAGTGGGCTGTAATGTTGATGGCCGCCATCCTCATGACATCATCGATGACATAACAGT; GGGTGCTTGTGGAATGCCCAGCCAGTTAAGCACAAAGGAAAACATTTAATAAAGGATCAT; TTGACAAAAAAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000976 unedited; NTTTAGCTTGNCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTCAAAT; GATCCTTTATTGAAATGTTTTCCTTTGTGCTTAACTGGCTGGGCATTCCACAGCACCACT; GTTGATGTCATCGATGATGTCATGAGGATGGCGGCCATCAACATTACAGCCCACTGACTG; GGCAGTCCCCAGGATCTCTTTAATGGTTCCAGAGAGTTCTCTGGCTAAGGATCGGTGCCG; CATCTGTCGAGCAATGTTGACAATCTCATCAAAAGTGATATTCCCACTGTGTTTAATGTT; TTTCTGTTTCTTTCTGTCTCTTGGTGGTTCCTTGAGGGCTTTGATGATCAGGGCAGAGGC; AGAAGGCACCACCTCAATCTGGGCCTGTCTGTTCTGAATGGTCAGTTTCACTGTAATCCT; CAGGCCCTTCCAGTCACCCGTTGCCTTGGCAATGTCATCACCAACTTTTTTTGGAGACAG; ACCCAGGGGGCCGATCTTGGGGGCCAGGGCAGAAGTGGCACCGACTTCACCTCCGGTGCA; CCTCAGGTATACGACTTTGATCTCGTTGGGGTCGAACTTCGGCGGCATGGTGGAGGCGGC; TGGTGTCGGATGAACCCGGATTCGGGACGACCGAAGGAAGTTGCACCTTGGCCTCGTGCC; GAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAG; CTCTGCTTATATAGACCTNCCACCGTACACGCCTACCGNCCATTTGCGTCAACGGGCGGG; GTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAACANACTNCCATTGACG; TCATGGGGTGGAGACTTGGAAATCCCGTGAGTCAACCGCTTCCACCCCATTGGTTACTGC; CAAAACGN;
• Restriction Sites: NotI-NotI
• ACCN: NM_000976
• Insert Size: 1920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000976.2, NP_000967.1
• RefSeq Size: 632 bp
• RefSeq ORF: 498 bp
• Locus ID: 6136
• UniProt ID: P30050
• Cytogenetics: 9q33.3
• Domains: Ribosomal_L11
• Protein Pathways: Ribosome
• Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. The protein binds directly to the 26S rRNA. This gene is co-transcribed with the U65 snoRNA, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]