Prostaglandin F2 alpha Receptor (PTGFR) (NM_000959) Human Untagged Clone

SKU SC119562

PTGFR (untagged)-Human prostaglandin F receptor (FP) (PTGFR), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Prostaglandin F2 alpha Receptor
• Synonyms: FP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119562 sequence for NM_000959 edited (data generated by NextGen Sequencing); ATGTCCATGAACAATTCCAAACAGCTAGTGTCTCCTGCAGCTGCGCTTCTTTCAAACACA; ACCTGCCAGACGGAAAACCGGCTTTCCGTATTTTTTTCAGTAATCTTCATGACAGTGGGA; ATCTTGTCAAACAGCCTTGCCATCGCCATTCTCATGAAGGCATATCAGAGATTTAGACAG; AAGTCCAAGGCATCGTTTCTGCTTTTGGCCAGTGGCCTGGTAATCACTGATTTCTTTGGC; CATCTCATCAATGGAGCCATAGCAGTATTTGTATATGCTTCTGATAAAGAATGGATCCGC; TTTGACCAATCAAATGTCCTTTGCAGTATTTTTGGTATCTGCATGGTGTTTTCTGGTCTG; TGCCCACTTCTTCTAGGCAGTGTGATGGCCATTGAGCGGTGTATTGGAGTCACAAAACCA; ATATTTCATTCTACGAAAATTACATCCAAACATGTGAAAATGATGTTAAGTGGTGTGTGC; TTGTTTGCTGTTTTCATAGCTTTGCTGCCCATCCTTGGACATCGAGACTATAAAATTCAG; GCGTCGAGGACCTGGTGTTTCTACAACACAGAAGACATCAAAGACTGGGAAGATAGATTT; TATCTTCTACTTTTTTCTTTTCTGGGGCTCTTAGCCCTTGGTGTTTCATTGTTGTGCAAT; GCAATCACAGGAATTACACTTTTAAGAGTTAAATTTAAAAGTCAGCAGCACAGACAAGGC; AGATCTCATCATTTGGAAATGGTAATCCAGCTCCTGGCGATAATGTGTGTCTCCTGTATT; TGTTGGAGCCCATTTCTGGTTACAATGGCCAACATTGGAATAAATGGAAATCATTCTCTG; GAAACCTGTGAAACAACACTTTTTGCTCTCCGAATGGCAACATGGAATCAAATCTTAGAT; CCTTGGGTATATATTCTTCTACGAAAGGCTGTCCTTAAGAATCTCTATAAGCTTGCCAGT; CAATGCTGTGGAGTGCATGTCATCAGCTTACATATTTGGGAGCTTAGTTCCATTAAAAAT; TCCTTAAAGGTTGCTGCTATTTCTGAGTCACCAGTTGCAGAGAAATCAGCAAGCACCTAG; ; Clone variation with respect to NM_000959.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000959 unedited; AGTGCGCGGAGGGGACGAGCGGCTGGACCACAGCCGGCGCCCGATCAGGATCTCCGCGCT; GGGATCGGTGGAACTTGAGGCAGCGGCGGCGCGGGGCGCCATGGCACACCGAGCGGCTCC; GTCTTCTGCTCCTCAGAGAGCCCGGCTGGCGGCCTGGGATGACAAGATGTCTGGACTGCA; ATCCTGCACAGTTTTGAGAGGGAGATGACTTGAGTGGTTGGCTTTTATCTCCACAACAAT; GTCCATGAACAATTCCAAACAGCTAGTGTCTCCTGCAGCTGCGCTTCTTTCAAACACAAC; CTGCCAGACGGAAAACCGGCTTTCCGTATTTTTTTCAGTAATCTTCATGACAGTGGGAAT; CTTGTCAAACAGCCTTGCCATCGCCATTCTCATGAAGGCATATCAGAGATTTAGACAGAA; GTCCAAGGGCATCGTTTCTGCTTTTGGCCAGGGGCCTGGTAATCACTGATTTCTTTGGCC; ATCTCATCAATGGAGCCATAGCAGTATGTGTATATGCTTCTGATAAAGAATGGATCCGCT; TTGACCAATCAAATGTCCCTTGCAGTATTTTTTGGGATCTGCATGGTGTTTTCTGGTCTG; TGCCCACTTCTTCTAGGCAGGGGGATGGGCATTGAGCGGGGTATTGAAGTCCCAAAACCA; TATTTCATTCTACAAAATTCCTCCAAACATGTGAAATGATGTTAAGGGGGTGTGCCTGTT; GCCGGGTTAATAGCTTTGCTGGCCCTCCTTGGACCTCGAGACTTTAAATTCACGGCTGGA; AGACCCNGGGGTTTTCCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000959 unedited; NTTTTAGCTNTGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTAAACATTAGTCCCAACTTTATTATAAAATTACTTGTTTGAAGTT; TTAAGCTAGTCAAAGCACTAAAATTACATGAGTTGCATACCAAAGCAAACAACAAAATAT; ACATATCACTATTTAATCTCTATACAATCCAACAAGTATGAAGAGCACAACATACATTTA; TTCACATAACCAAAAAAAACATGTTTCATCTACTATTAAAATGGGCCAGTAACAAGTTGA; AGTCCAACATTATTTACCAGGGGGTAAAATCTTTGACACCCAGATTAAAAAGTAACAAAA; TGTATATTCCCACCATTTATATTCTGACCCAAAACAGTTATTTAATTTGGAACAATGATG; GTATACAGGGGAAGCTAGCAATAGTTTTAACAAAAAGCACATTCTATATTTAACAAGTCC; TCTTATTTGGGTTCTAAACTCAAAGCAGGCACAAAACAAACCAGATTTCTCAAGCCCAAA; AGGGGGCAAACAAGAAACATTTTTCACTTAACAAAATATATCCACCTATGGATGTACTAA; CTTTCAGGGCTTTTTCGTTTTACCTTTATACATCCTTACAAATACCCTTTTGGCCCTAGG; GAAAAAAAAAAAAGGCGCCATTTTTTGCGCGAGGTAACCCCTCCCCCAACGGCCCCCCTT; TCTGCCCCACCCCCCGCCAATCGGCCACCTGGCCTTCTCCCTCGTCCGCAACGCGCAAAA; ACCCTCTGGCGGAACCTCCGCGGCTTGTGGGGCCACTTTTTCACGAAAACAGGCCATGAG; CCGTGCGAAAACCCTCAACTGCGGACGGTAGTGTGGGGCAACAATAACACGCGCGGAAAA; GTTTGTTGAACCCCGCCGCGACCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_000959
• Insert Size: 5310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000959.2, NP_000950.1
• RefSeq Size: 3466 bp
• RefSeq ORF: 1080 bp
• Locus ID: 5737
• UniProt ID: P43088
• Cytogenetics: 1p31.1
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Summary: The protein encoded by this gene is member of the G-protein coupled receptor family. This protein is a receptor for prostaglandin F2-alpha (PGF2-alpha), which is known to be a potent luteolytic agent, and may also be involved in modulating intraocular pressure and smooth muscle contraction in uterus. Knockout studies in mice suggest that the interaction of PGF2-alpha with this receptor may initiate parturition in ovarian luteal cells and thus induce luteolysis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longer isoform (a).