EP4 (PTGER4) (NM_000958) Human Untagged Clone

SKU SC119561

PTGER4 (untagged)-Human prostaglandin E receptor 4 (subtype EP4) (PTGER4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: EP4
• Synonyms: EP4; EP4R
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119561 sequence for NM_000958 edited (data generated by NextGen Sequencing); ATGTCCACTCCCGGGGTCAATTCGTCCGCCTCCTTGAGCCCCGACCGGCTGAACAGCCCA; GTGACCATCCCGGCGGTGATGTTCATCTTCGGGGTGGTGGGCAACCTGGTGGCCATCGTG; GTGCTGTGCAAGTCGCGCAAGGAGCAGAAGGAGACGACCTTCTACACGCTGGTATGTGGG; CTGGCTGTCACCGACCTGTTGGGCACTTTGTTGGTGAGCCCGGTGACCATCGCCACGTAC; ATGAAGGGCCAATGGCCCGGGGGCCAGCCGCTGTGCGAGTACAGCACCTTCATTCTGCTC; TTCTTCAGCCTGTCCGGCCTCAGCATCATCTGCGCCATGAGTGTCGAGCGCTACCTGGCC; ATCAACCATGCCTATTTCTACAGCCACTACGTGGACAAGCGATTGGCGGGCCTCACGCTC; TTTGCAGTCTATGCGTCCAACGTGCTCTTTTGCGCGCTGCCCAACATGGGTCTCGGTAGC; TCGCGGCTGCAGTACCCAGACACCTGGTGCTTCATCGACTGGACCACCAACGTGACGGCG; CACGCCGCCTACTCCTACATGTACGCGGGCTTCAGCTCCTTCCTCATTCTCGCCACCGTC; CTCTGCAACGTGCTTGTGTGCGGCGCGCTGCTCCGCATGCACCGCCAGTTCATGCGCCGC; ACCTCGCTGGGCACCGAGCAGCACCACGCGGCCGCGGCCGCCTCGGTTGCCTCCCGGGGC; CACCCCGCTGCCTCCCCAGCCTTGCCGCGCCTCAGCGACTTTCGGCGCCGCCGGAGCTTC; CGCCGCATCGCGGGCGCCGAGATCCAGATGGTCATCTTACTCATTGCCACCTCCCTGGTG; GTGCTCATCTGCTCCATCCCGCTCGTGGTGCGAGTATTCGTCAACCAGTTATATCAGCCA; AGTTTGGAGCGAGAAGTCAGTAAAAATCCAGATTTGCAGGCCATCCGAATTGCTTCTGTG; AACCCCATCCTAGACCCCTGGATATATATCCTCCTGAGAAAGACAGTGCTCAGTAAAGCA; ATAGAGAAGATCAAATGCCTCTTCTGCCGCATTGGCGGGTCCCGCAGGGAGCGCTCCGGA; CAGCACTGCTCAGACAGTCAAAGGACATCTTCTGCCATGTCAGGCCACTCTCGCTCCTTC; ATCTCCCGGGAGCTGAAGGAGATCAGCAGTACATCTCAGACCCTCCTGCCAGACCTCTCA; CTGCCAGACCTCAGTGAAAATGGCCTTGGAGGCAGGAATTTGCTTCCAGGTGTGCCTGGC; ATGGGCCTGGCCCAGGAAGACACCACCTCACTGAGGACTTTGCGAATATCAGAGACCTCA; GACTCTTCACAGGGTCAGGACTCAGAGAGTGTCTTACTGGTGGATGAGGCTGGTGGGAGC; GGCAGGGCTGGGCCTGCCCCTAAGGGGAGCTCCCTGCAAGTCACATTTCCCAGTGAAACA; CTGAACTTATCAGAAAAATGTATATAA; ; Clone variation with respect to NM_000958.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000958 unedited; CGCACGGGGGGAGNCGCTACTTTCACNNGGNGGTTCGGAATTGAAACGNATTATATAGGC; GGACGCCAATTCGCACCAGCCGGCAGCCCGTATAGGAAGATGAACAGCCCCAGGCCAGAG; CCTCTGGCAGAGTGGACCCCGAGCCGCCCCCAGGTAGCCAGGAGCGGCCTCAGCGGCAGC; CGCAAACTCCAGTAGCCGCCCGTGCTGCCCGTGGCTGGGGCGGAGGGCAGCCAGAGCTGG; GGACTTTTTGTTCCGCGCCACCTGCGCGCACAGCCTCACACCTGAACGCTGTCCTCCCGC; AGACGAGACCGGCGGGCACTGCAAAGCTGGGACTCGTCTTTGAAGGAAAAAAAATAGCGA; GTAAGAAATCCAGCACCATTCTTCACTGACCCATCCCGCTGCACCTCTTGTTTCCCAAGT; TTTTGAAAGCTGGCAACTCTGACCTCGGTGTCCAAAAATCGACAGCCACTGAGACCGGCT; TTGAGAAGCCGAAGATTTGGCAGTTTCCAGACTGAGCAGGACAAGGTGAAAGCAGGTTGG; AGGCGGGTCCAGGACATCTGAGGGCTGACCCTGNGGGCTCGTGAGGCTGCCACCGCTGCT; GCCGCTACAGACCCAGCCTTGCACTCCAAGGCTGCGCACCGCCAGCCACTATCATGTCCA; CTCCCCGGGGTCAATTCGTCCGCCTCCTTGAGCCCCGACCGGCTGAACAGCCCCATGACC; ATCCCCGCGGGTGATGTTCATCTTCCGGGGTGGGTGGGCAACCTGGTGGGCATCGTGGNG; GCTGTGCCAAGTCGCGCAAGGAGCCAAAAGGAAACGACCTTTTTACACCCTTGGTATGTG; GGGCTGGGCTGTCACCGCACCTGTTGGGCCACTTTGTTTGTGGAGCCCGGTGGACCATTG; CCACGTACATGTAGGGGCCAATGCGCCCGGGGGCCAACCCGTTGGGCCAATCCACACACC; TTTATTCGGCTCTTCTTAAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000958 unedited; NNTTATTACTTGNCCGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTCAGAT; TGAATGTATATATTTATTATAATCTCCAAATAATTTCACTTGGTACAACTGCTTCTTAAA; ACCATATCAATATCAGGCTCAGAATTTAATTACAACCAAGCAATTCACAAAAACACTGAG; CAACAAAACATGCTTAATATTTCTTTGAGAAAGACCCTTCAAATATGTGTACAGCATCAC; TGGGAGTTACACAAAACTGTTACAAGGTGACCATTAAGTGCCCCAATTCTGCACTTCTGA; CATACATGAATGGCTAATGTAACCACGTTTGGGAATCTTTTTACATCTCAAAATAAAGCT; TTCTGATGCAACTTGCCATCCTTTTAAATTTTAAAGGATATTCTTGGTAATTCCTTAGGA; AAGTAAAACTACACACACTTTCAGAGAAACAATAAGCTGCTTAGATTTTTAAAATTTTTT; ATATTATACACTTCAATTATGGGTTATTTATTAAAGACTTCAAAAAATTTATGTTATGTT; TTTAAAAGTACCAATTAGTGAGGGAAAAAGTTGAAAATATTCACCAGCATGTTAGGTTCT; CCTGAAATCACAGTTTTCTGGATAAATAAATGTAATATCACTAAACAATTTTCACTATGT; ATTTTATACATAGCCTGGAAAGAACAGTATTTTGGACTGTAATTTTCACATCTAANATAG; TATTTTTTTCTTTATCCCTTTGATATACTAANNATCTTCTCTTAAAAATTATATCTATTT; TATAGATATCAATCTTACTATGAATNTTCAACTTTGACTCGAACTTGNNTATGAAAATAT; CTAACATCTGCACTTCAGCTGGTATACTGGAAGAAGAGGGTTGCGGATATTTATATACCN; TGGATAACAATAACCNNTCTACAGAGAGAAATACTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_000958
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000958.2, NP_000949.1
• RefSeq Size: 3432 bp
• RefSeq ORF: 1467 bp
• Locus ID: 5734
• UniProt ID: P35408
• Cytogenetics: 5p13.1
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Summary: The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor can activate T-cell factor signaling. It has been shown to mediate PGE2 induced expression of early growth response 1 (EGR1), regulate the level and stability of cyclooxygenase-2 mRNA, and lead to the phosphorylation of glycogen synthase kinase-3. Knockout studies in mice suggest that this receptor may be involved in the neonatal adaptation of circulatory system, osteoporosis, as well as initiation of skin immune responses. [provided by RefSeq, Jul 2008]