Amino terminal enhancer of split (AES) (NM_001130) Human Untagged Clone

SKU SC119415

AES (untagged)-Human amino-terminal enhancer of split (AES), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Amino terminal enhancer of split
• Synonyms: AES; AES-1; AES-2; ESP1; GRG; Grg-5; GRG5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119415 sequence for NM_001130 edited (data generated by NextGen Sequencing); ATGATGTTTCCACAAAGCAGGCATTCGGGCTCCTCGCACCTACCCCAGCAACTCAAATTC; ACCACCTCGGACTCCTGCGACCGCATCAAAGACGAATTTCAGCTACTGCAAGCTCAGTAC; CACAGCCTCAAGCTCGAATGTGACAAGTTGGCCAGTGAGAAGTCAGAGATGCAGCGTCAC; TATGTGATGTACTACGAGATGTCCTACGGCTTGAACATCGAGATGCACAAACAGGCTGAG; ATCGTCAAAAGGCTGAACGGGATTTGTGCCCAGGTCCTGCCCTACCTCTCCCAAGAGCAC; CAGCAGCAGGTCTTGGGAGCCATTGAGAGGGCCAAGCAGGTCACCGCTCCCGAGCTGAAC; TCTATCATCCGACAGCAGCTCCAAGCCCACCAGCTGTCCCAGCTGCAGGCCCTGGCCCTG; CCCTTGACCCCACTACCCGTGGGGCTGCAGCCGCCTTCGCTGCCGGCGGTCAGCGCAGGC; ACCGGCCTCCTCTCGCTGTCCGCGCTGGGTTCCCAGGCCCACCTCTCCAAGGAAGACAAG; AACGGGCACGATGGTGACACCCACCAGGAGGATGATGGCGAGAAGTCGGATTAG; ; Clone variation with respect to NM_001130.5;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001130 unedited; TAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCGATCGGCGCCGGGACAA; AGGCGCGGCCGCCCGGCGCCCCCAGCAGCCCGAGCCGGGGCGCACAGCCGGGGCGCAGCC; CGCGCCCCCCGCCGCGATTGACATGATGTTTCCACAAAGCAGGCATTCGGGCTCCTCGCA; CCTACCCCAGCAACTCAAATTCACCACCTCGGACTCCTGCGACCGCATCAAAGACGAATT; TCAGCTACTGCAAGCTCAGTACCACAGCCTCAAGCTCGAATGTGACAAGTTGGCCAGTGA; GAAGTCAGAGATGCAGCGTCACTATGTGATGTACTACGAGATGTCCTACGGCTTGAACAT; CGAGATGCACAAACAGGCTGAGATCGTCAAAAGGCTGAACGGGATTTGTGCCCAGGTCCT; GCCCTACCTCTCCCAAGAGCACCAGCAGCAGGTCTTGGGAGCCATTGAGAGGGCCAAGCA; GGTCACCGCTCCCGAGCTGAACTCTATCATCCGACAGCAGCTCCAAGCCCACCAGCTGTC; CCAGCTGCAGGCCCTGGCCCTGCCCTTGACCCCACTACCCGTGGGGCTGCAGCCGCCTTC; GCTGCCGGCGGTCAGCGCAGGCACCGGCCTNCTCTCGCTGTCCGCGCTGGGTTCCCAGGC; CCACCTCTCCAGGGAGACAGAACGGGCACGATGGTGACACCCACCAGGAGGATGATGGCG; AGAAAGTCGATTANCAAGGNGGCCGGACGGNGAGGGTTGGAGGGNGGACCAGAGGGANAC; AGAGGCACNGAGAGAAAAGGATGTTTATCACAAGACACAGCGGAGCTCGGGGATGGNCTA; ACTCCATAGTATTTATGGTGGCCGNNCGCGNNGGCCCCAGCCAGCTTGCAAGCCCACTNT; AGCTTTNTTNNCTACCCCATACGGNNNTTCTTCCTCCTCCCNTGCAGNCTGGNTATGTGG; ATACCTGCCCTGACTGGTTGAGCAAGCTAGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001130 unedited; CGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAACT; TCTCACATCTTTATTGGAAAGGCACAGCTAAGCCCACCCTTGATACAGCATTTTCCACTT; CTCTCTGTAGAGATCAGACGATTGAACACGAGATAAGCTGACTATATACAGACACAGGTG; TGGGTGTTGCTTTTTTTTAAAAACACTTTTGGCTTTTTTTTTTTTTAATATCCCCTTTCT; TAAAAGACAAGCTAGTATACTGGAAAAAGAAAAAAATAATAATAAAATAAAAACCAAGAC; AACTTTAGTACCCTCATCTTTATTTGGGAAGGGGAGGGGGAATCCTGGGTCGCCCACCCT; TACCCTGGTCCTCCCAGCTCAGCTAAGCTCGTCCCTCGTGCCCCCCCTTTTGTGGGCGAT; GGGAAAGGACCAGGTGGGCGTGGAGGTGTCTGGAACTAACAAAGGTGGTGAGTGGGGCAA; GTGGAGGTGGGAGCATACCTGGGACCCGGGGTCGGGGGAGACTCGGGGTGCCCAGGACGG; GAAAGGGGCAGCTAGCATTGCGTGCATGCAGTACCAGGGTGAGAGGGCTGTGGCCCAGGC; AGACTGTCGGTTACACATGTTCAAAACGGGGGAAGGGCCGGGGCTGCTGCGCTTCGCGAG; GTCTTGCTCCCTTGGGACCTGGTCTCCCATCTGACCCTCCAGGCCTTAGCTTGCCTCACA; CGTCAGGGCAGGTATCCACCTAACGGNCTGCAGGGGAGGAGGAGGGAANCCCGGATGGGG; TANGAAAAAGCTANAGTGGCCTGCAACTGGCTGGGGCCCCGCCGCGGCCCATAATACTTG; GGATTATCCATCCGACTCGCTGGTCTGGCTAAATTCTCTTCGCGCCCGGGTCCCCCTTGC; CCCCTTCAACTCCGGTCGGCCCTGTATACGATTCGCTATCCCTGGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001130
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001130.5, NP_001121.2
• RefSeq Size: 1687 bp
• RefSeq ORF: 594 bp
• Locus ID: 166
• UniProt ID: Q08117
• Cytogenetics: 19p13.3
• Domains: TLE_N
• Protein Families: Druggable Genome, Transcription Factors
• Summary: The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b) is shorter and has a distinct N-terminus compared to isoform a.