Bak (BAK1) (NM_001188) Human Untagged Clone

CAT#: SC119385

BAK1 (untagged)-Human BCL2-antagonist/killer 1 (BAK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Bak
• Synonyms: BAK; BAK-LIKE; BCL2L7; CDN1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001188 edited
  GAATTCGGCACGAGGCACAGGGACAAGTAAAGGCTACATCCAGATGCCGGGAATGCACTG
  ACGCCCATTCCTGGAAACTGGGCTCCCACTCAGCCCCTGGGAGCAGCAGCCGCCAGCCCC
  TCGGGACCTCCATCTCCACCCTGCTGAGCCACCCGGGTTGGGCCAGGATCCCGGCAGGCT
  GATCCCGTCCTCCACTGAGACCTGAAAAATGGCTTCGGGGCAAGGCCCAGGTCCTCCCAG
  GCAGGAGTGCGGAGAGCCTGCCCTGCCCTCTGCTTCTGAGGAGCAGGTAGCCCAGGACAC
  AGAGGAGGTTTTCCGCAGCTACGTTTTTTACCGCCATCAGCAGGAACAGGAGGCTGAAGG
  GGTGGCTGCCCCTGCCGACCCAGAGATGGTCACCTTACCTCTGCAACCTAGCAGCACCAT
  GGGGCAGGTGGGACGGCAGCTCGCCATCATCGGGGACGACATCAACCGACGCTATGACTC
  AGAGTTCCAGACCATGTTGCAGCACCTGCAGCCCACGGCAGAGAATGCCTATGAGTACTT
  CACCAAGATTGCCACCAGCCTGTTTGAGAGTGGCATCAATTGGGGCCGTGTGGTGGCTCT
  TCTGGGCTTCGGCTACCGTCTGGCCCTACACGTCTACCAGCATGGCCTGACTGGCTTCCT
  AGGCCAGGTGACCCGCTTCGTGGTCGACTTCATGCTGCATCACTGCATTGCCCGGTGGAT
  TGCACAGAGGGGTGGCTGGGTGGCAGCCCTGAACTTGGGCAATGGTCCCATCCTGAACGT
  GCTGGTGGTTCTGGGTGTGGTTCTGTTGGGCCAGTTTGTGGTACGAAGATTCTTCAAATC
  ATGACTCCCAAGGGTGCCCTTTGGGGTCCCGGTTCAXXXXXXXXXXXXXXXXXXXAGGTC
  CTGCTCAACTCTACCCCTGCTCCCATTCCTCCCTCCGGCCATACTGCCTTTGCAGTTGGA
  CTCTCAGGGATTCTGGGCTTGGGGTGTGGGGTGGGGTGGAGTCGCAGACCAGAGCTGTCT
  GAACTCACGTGTCAGAAGCCTCCAAGCCTGCCTCCCAAGGTCCTCTCAGTTCTCTCCCTT
  CCTCTCTCCTTATAGACACTTGCTCCCAACCCATTCACTACAGGTGAAGGCTCTCACCCC
  CATCCCTGGGGGCCTTGGGTGAGTGGCCTGCTAAGGCTCCTCCTTGCCCAGACTACAGGG
  CTTAGGACTTGGTTTGTTATTTCAGGGAAAAGGAGTAGGGAGTTCATCTGGAGGGTTCTA
  AGTGGGAGAAGGACTATCAACACCACTAGGAATCCCAGAGGTGGGATCCTCCCTCATGGC
  TCTGGCACAGTGTAATCCAGGGGTGTAGATGGGGGAACTGTGAATACTTGAACTCTGTTC
  CCCCACCCTCCATGCTCCTCACCTGTCTAGGTCTCCTCAGGGTGGGGGGTGACAGTGCCT
  TCTCTATTGGGCACAGCCTAGGGTCTTGGGGGTCAGGGGGGAGAAGTTCTTGATTCAGCC
  AAATGCAGGGAGGGGAGGCAGATGGAGCCCATAGGCCACCCCCTATCCTCTGAGTGTTTG
  GAAATAAACTGTGCAATCCCCTCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001188 unedited
  GGTTATAATTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCACAGG
  GACAAGTAAAGGCTACATCCAGATGCCGGGAATGCACTGACGCCCATTCCTGGAAACTGG
  GCTCCCACTCAGCCCCTGGGAGCAGCAGCCGCCAGCCCCTCGGGACCTCCATCTCCACCC
  TGCTGAGCCACCCGGGTTGGGCCAGGATCCCGGCAGGCTGATCCCGTCCTCCACTGAGAC
  CTGAAAAATGGCTTCGGGGCAAGGCCCAGGTCCTCCCAGGCAGGAGTGCGGAGAGCCTGC
  CCTGCCCTCTGCTTCTGAGGAGCAGGTAGCCCAGGACACAGAGGAGGTTTTCCGCAGCTA
  CGTTTTTTACCGCCATCAGCAGGAACAGGAGGCTGAAGGGGTGGCTGCCCCTGCCGACCC
  AGAGATGGTCACCTTACCTCTGCAACCTAGCAGCACCATGGGGCAGGTGGGACGGCAGCT
  CGCCATCATCGGGGACGACATCAACCGACGCTATGACTCAGAGTTCCAGACCATGTTGCA
  GCACCTGCAGCCCACGGCAGAGAATGCCTATGAGTACTTCACCAAGATTGCCACCAGCCT
  GTTTGAGAGTGGCATCAATTGGGGCCGTGTGGTGGCTCTTCTGGGCTTCGGCTACCGTCT
  GGCCCTACACGTCTACCAGCATGCCCTGACTGGCTTCCTANGCCANGTGACCCGCTTCGT
  GGTCGACTTCATGCTGCATCACTGCATTGCCCGGTGGATTGCACACAGGGCTGCCTGGGT
  GGCAGCCCTGAACTTTGGCCATGGTCCCATCCTGAACGTGCTGGTGGNTCCTGGGGTGGG
  TCTTGTGGGCCCAGTTGTGTTACGAAAAATCTCAAACATGACTCCCAAGGTGCCCTTTGG
  GGTCCCGGTCAGACCCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001188 unedited
  ATGGCCGCGGCCCGCATCTANGATCGAGTTTTTTTTTTTTTTTTTTGAGGGGATTGCACA
  GTTTATTTCCAAACACTCAGNAGATAGGGGGTGGCCTATGGGCTCCATCTGCCTCCCCTC
  CCTGCATTTGGCTGAATCAAGAACTTCTCCCCCCTGACCCCCAAGACCCTAGGCTGTGCC
  CAATAGAGAAGGCACTGTCACCCCCCACCCTGAGGAGACCTAGACAGGTGAGGAGCATGG
  AGGGTGGGGGAACAGAGTTCAAGTATTCACAGTTCCCCCATCTACACCCCTGGATTACAC
  TGTGCCAGAGCCATGAGGGAGGATCCCACCTCTGGGATTCCTAGTGGCGTTGATAGTCCT
  TCTCCCACTTAGAACCCTCCAGATGAACTCCCTACTCCTTTTCCCTGAAATAACAAACCA
  AGTCCTAAGCCCTGTAGTCTGGGCAAGGAGGAGCCTTAGCAGGCCACTCACCCAAGGCCC
  CCAGGGATGGGGGTGAGAGCCCTTCACCTGTAGTGAATGGGTTGGGAGCAAGTGTCTATA
  AGGAGAGAGGAAGGGAGAGAACTGACAGGACCTTGGGAGGCAGGCTTGGAGGCTTCTGAC
  ACGTGAGTTCAGACAGCTCTGGTCTGCGACTTCACCCCACCCACACCCCAAGCCCAGAAT
  CCCTGAGAGTTCAACTGCAAAGGCCATATGGCCGGAGGGAGGAATGGCAGCCAGGGTAAA
  GTTGACCAGGACCTTGATCACCCTTTACTAAAGGACCTGCTCCTGGGCACATGCTTAGGG
  ACGCCCAGCACCAGCCTCAGCTCCTAGACCCATCTCTTAGGGGCTGATCTTACAAAAACC
  TGAAATCTGGGGAACATAAACCCCCCATTTCCGACCCCCCGAAGACCTGGACCCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001188
• Insert Size: 2130 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001188.2, NP_001179.1
• RefSeq Size: 2165 bp
• RefSeq ORF: 636 bp
• Locus ID: 578
• UniProt ID: Q16611
• Cytogenetics: 6p21.31
• Domains: Bcl-2
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transmembrane
• Gene Summary: The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008]