ATP6AP1 (NM_001183) Human Untagged Clone

CAT#: SC119382

ATP6AP1 (untagged)-Human ATPase, H+ transporting, lysosomal accessory protein 1 (ATP6AP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP6AP1
• Synonyms: 16A; Ac45; ATP6IP1; ATP6S1; CF2; VATPS1; XAP-3; XAP3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001183 edited
  GAATTCGGCACCAGGAGGCTATGATGGCGGCCATGGCGACGGCTCGAGTGCGGATGGGGC
  CGCGATGCGCCCAGGCGCTCTGGCGCATGCCGTGGCTGCCGGTGTTTTTGTCGTTGGCGG
  CGGCGGCGGCGGCGGCAGCGGCGGAGCAGCAGGTCCCGCTGGTGCTGTGGTCGAGTGACC
  GGGACTTGTGGGCTCCTGCGGCCGACACTCATGAAGGCCACATCACCAGCGACTTGCAGC
  TCTCTACCTACTTAGATCCCGCCCTGGAGCTGGGTCCCAGGAATGTGCTGCTGTTCCTGC
  AGGACAAGCTGAGCATTGAGGATTTCACAGCATATGGCGGTGTGTTTGGAAACAAGCAGG
  ACAGCGCCTTTTCTAACCTAGAGAATGCCCTGGACCTGGCCCCCTCCTCACTGGTGCTTC
  CTGCCGTCGACTGGTATGCAGTCAGCACTCTGACCACTTACCTGCAGGAGAAGCTCGGGG
  CCAGCCCCTTGCATGTGGACCTGGCCACCCTGCGGGAGCTGAAGCTCAATGCCAGCCTCC
  CTGCTCTGCTGCTCATTCGCCTGCCCTACACAGCCAGCTCTGGTCTGATGGCACCCAGGG
  AAGTCCTCACAGGCAACGATGAGGTCATCGGGCAGGTCCTGAGCACACTCAAGTCCGAAG
  ATGTCCCATACACAGCGGCCCTCACAGCGGTCCGCCCTTCCAGGGTGGCCCGTGATGTAG
  CCGTGGTGGCCGGAGGGCTAGGTCGCCAGCTGCTACAAAAACAGCCAGTATCACCTGTGA
  TCCATCCTCCTGTGAGTTACAATGACACCGCTCCCCGGATCCTGTTCTGGGCCCAAAACT
  TCTCTGTGGCGTACAAGGACCAGTGGGAGGACCTGACTCCCCTCACCTTTGGGGTGCAGG
  AACTCAACCTGACTGGCTCCTTCTGGAATGACTCCTTTGCCAGGCTCTCACTGACCTATG
  AACGACTCTTTGGTACCACAGTGACATTCAAGTTCATTCTGGCCAACCGCCTCTACCCAG
  TGTCTGCCCGGCACTGGTTTACCATGGAGCGCCTCGAAGTCCACAGCAATGGCTCCGTCG
  CCTACTTCAATGCTTCCCAGGTCACAGGGCCCAGCATCTACTCCTTCCACTGCGAGTATG
  TCAGCAGCCTGAGCAAGAAGGGTAGTCTCCTCGTGGCCCGCACGCAGCCCTCTCCCTGGC
  AGATGATGCTTCAGGACTTCCAGATCCAGGCTTTCAACGTAATGGGGGAGCAGTTCTCCT
  ACGCCAGCGACTGTGCCAGCTTCTTCTCCCCCGGCATCTGGATGGGGCTGCTCACCTCCC
  TGTTCATGCTCTTCATCTTCACCTATGGCCTGCACATGATCCTCAGCCTCAAGACCATGG
  ATCGCTTTGATGACCACAAGGGCCCCACTATTTCTTTGACCCAGATTGTGTGACCCTGTG
  CCAGTGGGGGGGTTGAGGGTGGGACGGTGTCCGTGTTGTTGCTTTCCCACCCTGCAGCGC
  ACTGGACTGAAGAGCTTCCCTCTTCCTACTGCAGCATGAACTGCAAGCTCCCCTCAGCCC
  ATCTTGCTCCCTCTTCAGCCCGCTGAGGAGCTTTCTTGGGCTGCCCCCATCTCTCCCAAC
  AAGGTGTACATATTCTGCGTAGATGCTAGACCAACCAGCTTCCCAGGGTTCGTCGCTGTG
  AGGCGTAAGGGACGTGAATTCTAGGGTCTCCTTTCTCCTTATTTATTCTTGTGGCTACAT
  CATCCCTGGCTGTGGATAGTGCTTTTGTGTAGCAAATGCTCCCTCCTTAAGGTTATAGGG
  CTCCCTGAGTTTGGGAGTGTGGAAGTACTACTTAACTGTCTGTCCTGCTTGGCTGTCGTT
  ATCGTTTTCTGGTGATGTTGTGCTAACAATAAGCAGTACACGGGTTTATTTCTGTGGCCT
  GAGAAGGAAGGGACCTCCACGACAGGTGGGCTGGGTGCGATCGCCGGCTGTTTGGCATGT
  TCCCACCGGGAGTGCCGGGCAGGAGCATGGGGTGCTTGGTTGTTTCCTTCCTAATAAAAT
  AAACGCGGGTCGCCATGAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001183 unedited
  NTTGTTAGATTTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACCAGGAGGCTAT
  GATGGCGGCCATGGCGACGGCTCGAGTGCGGATGGGGCCGCGATGCGCCCAGGCGCTCTG
  GCGCATGCCGTGGCTGCCGGTGTTTTTGTCGTTGGCGGCGGCGGCGGCGGCGGCAGCGGC
  GGAGCAGCAGGTCCCGCTGGTGCTGTGGTCGAGTGACCGGGACTTGTGGGCTCCTGCGGC
  CGACACTCATGAAGGCCACATCACCAGCGACTTGCAGCTCTCTACCTACTTAGATCCCGC
  CCTGGAGCTGGGTCCCAGGAATGTGCTGCTGTTCCTGCAGGACAAGCTGAGCATTGAGGA
  TTTCACAGCATATGGCGGTGTGTTTGGAAACAAGCAGGACAGCGCCTTTTCTAACCTAGA
  GAATGCCCTGGACCTGGCCCCCTCCTCACTGGTGCTTCCTGCCGTCGACTGGTATGCAGT
  CAGCACTCTGACCACTTACCTGCAGGAGAAGCTCGGGGCCAGCCCCTTGCATGTGGACCT
  GGCCACCCTGCGGGAGCTGAAGCTCAATGCCAGCCTCCCTGCTCTGCTGCTCATTCGCCT
  GCCCTACACAGCCAGCTCTGGTCTGATGGCACCCAGGGAAGTCCTCACAGGCAACGATGA
  GGTCATCGGGCAGGTCCTGAGCACACTCAAGTCCGAAGATGTCCCATACACAGCGGCCCT
  CACAGCGGTCCGCCCTTCCAGGTGGCCCGTGATGTANCCGTGGTGGCCGGAGGGCTAGGT
  CGCCAGCTGCTACAAAACAGCCAGATCACCTGTGATCATNCTCCTGTGANTACATGACAC
  GCTCCCNNGGATCTGNTCTGGCCCAAACTTCTCTGTGCGTACAGGACANTGGNNAGACTG
  ACTCCTACTTTTGGGGGCAGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001183
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001183.3, NP_001174.2
• RefSeq Size: 2055 bp
• RefSeq ORF: 1413 bp
• Locus ID: 537
• UniProt ID: Q15904
• Cytogenetics: Xq28
• Protein Families: Transmembrane
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Gene Summary: This gene encodes a component of a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. This protein may also play a role in early development. [provided by RefSeq, Aug 2013]