ATP6AP1 (NM_001183) Human Untagged Clone

SKU SC119382

ATP6AP1 (untagged)-Human ATPase, H+ transporting, lysosomal accessory protein 1 (ATP6AP1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ATP6AP1
• Synonyms: 16A; Ac45; ATP6IP1; ATP6S1; CF2; VATPS1; XAP-3; XAP3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001183 edited; GAATTCGGCACCAGGAGGCTATGATGGCGGCCATGGCGACGGCTCGAGTGCGGATGGGGC; CGCGATGCGCCCAGGCGCTCTGGCGCATGCCGTGGCTGCCGGTGTTTTTGTCGTTGGCGG; CGGCGGCGGCGGCGGCAGCGGCGGAGCAGCAGGTCCCGCTGGTGCTGTGGTCGAGTGACC; GGGACTTGTGGGCTCCTGCGGCCGACACTCATGAAGGCCACATCACCAGCGACTTGCAGC; TCTCTACCTACTTAGATCCCGCCCTGGAGCTGGGTCCCAGGAATGTGCTGCTGTTCCTGC; AGGACAAGCTGAGCATTGAGGATTTCACAGCATATGGCGGTGTGTTTGGAAACAAGCAGG; ACAGCGCCTTTTCTAACCTAGAGAATGCCCTGGACCTGGCCCCCTCCTCACTGGTGCTTC; CTGCCGTCGACTGGTATGCAGTCAGCACTCTGACCACTTACCTGCAGGAGAAGCTCGGGG; CCAGCCCCTTGCATGTGGACCTGGCCACCCTGCGGGAGCTGAAGCTCAATGCCAGCCTCC; CTGCTCTGCTGCTCATTCGCCTGCCCTACACAGCCAGCTCTGGTCTGATGGCACCCAGGG; AAGTCCTCACAGGCAACGATGAGGTCATCGGGCAGGTCCTGAGCACACTCAAGTCCGAAG; ATGTCCCATACACAGCGGCCCTCACAGCGGTCCGCCCTTCCAGGGTGGCCCGTGATGTAG; CCGTGGTGGCCGGAGGGCTAGGTCGCCAGCTGCTACAAAAACAGCCAGTATCACCTGTGA; TCCATCCTCCTGTGAGTTACAATGACACCGCTCCCCGGATCCTGTTCTGGGCCCAAAACT; TCTCTGTGGCGTACAAGGACCAGTGGGAGGACCTGACTCCCCTCACCTTTGGGGTGCAGG; AACTCAACCTGACTGGCTCCTTCTGGAATGACTCCTTTGCCAGGCTCTCACTGACCTATG; AACGACTCTTTGGTACCACAGTGACATTCAAGTTCATTCTGGCCAACCGCCTCTACCCAG; TGTCTGCCCGGCACTGGTTTACCATGGAGCGCCTCGAAGTCCACAGCAATGGCTCCGTCG; CCTACTTCAATGCTTCCCAGGTCACAGGGCCCAGCATCTACTCCTTCCACTGCGAGTATG; TCAGCAGCCTGAGCAAGAAGGGTAGTCTCCTCGTGGCCCGCACGCAGCCCTCTCCCTGGC; AGATGATGCTTCAGGACTTCCAGATCCAGGCTTTCAACGTAATGGGGGAGCAGTTCTCCT; ACGCCAGCGACTGTGCCAGCTTCTTCTCCCCCGGCATCTGGATGGGGCTGCTCACCTCCC; TGTTCATGCTCTTCATCTTCACCTATGGCCTGCACATGATCCTCAGCCTCAAGACCATGG; ATCGCTTTGATGACCACAAGGGCCCCACTATTTCTTTGACCCAGATTGTGTGACCCTGTG; CCAGTGGGGGGGTTGAGGGTGGGACGGTGTCCGTGTTGTTGCTTTCCCACCCTGCAGCGC; ACTGGACTGAAGAGCTTCCCTCTTCCTACTGCAGCATGAACTGCAAGCTCCCCTCAGCCC; ATCTTGCTCCCTCTTCAGCCCGCTGAGGAGCTTTCTTGGGCTGCCCCCATCTCTCCCAAC; AAGGTGTACATATTCTGCGTAGATGCTAGACCAACCAGCTTCCCAGGGTTCGTCGCTGTG; AGGCGTAAGGGACGTGAATTCTAGGGTCTCCTTTCTCCTTATTTATTCTTGTGGCTACAT; CATCCCTGGCTGTGGATAGTGCTTTTGTGTAGCAAATGCTCCCTCCTTAAGGTTATAGGG; CTCCCTGAGTTTGGGAGTGTGGAAGTACTACTTAACTGTCTGTCCTGCTTGGCTGTCGTT; ATCGTTTTCTGGTGATGTTGTGCTAACAATAAGCAGTACACGGGTTTATTTCTGTGGCCT; GAGAAGGAAGGGACCTCCACGACAGGTGGGCTGGGTGCGATCGCCGGCTGTTTGGCATGT; TCCCACCGGGAGTGCCGGGCAGGAGCATGGGGTGCTTGGTTGTTTCCTTCCTAATAAAAT; AAACGCGGGTCGCCATGAAAAAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001183 unedited; NTTGTTAGATTTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACCAGGAGGCTAT; GATGGCGGCCATGGCGACGGCTCGAGTGCGGATGGGGCCGCGATGCGCCCAGGCGCTCTG; GCGCATGCCGTGGCTGCCGGTGTTTTTGTCGTTGGCGGCGGCGGCGGCGGCGGCAGCGGC; GGAGCAGCAGGTCCCGCTGGTGCTGTGGTCGAGTGACCGGGACTTGTGGGCTCCTGCGGC; CGACACTCATGAAGGCCACATCACCAGCGACTTGCAGCTCTCTACCTACTTAGATCCCGC; CCTGGAGCTGGGTCCCAGGAATGTGCTGCTGTTCCTGCAGGACAAGCTGAGCATTGAGGA; TTTCACAGCATATGGCGGTGTGTTTGGAAACAAGCAGGACAGCGCCTTTTCTAACCTAGA; GAATGCCCTGGACCTGGCCCCCTCCTCACTGGTGCTTCCTGCCGTCGACTGGTATGCAGT; CAGCACTCTGACCACTTACCTGCAGGAGAAGCTCGGGGCCAGCCCCTTGCATGTGGACCT; GGCCACCCTGCGGGAGCTGAAGCTCAATGCCAGCCTCCCTGCTCTGCTGCTCATTCGCCT; GCCCTACACAGCCAGCTCTGGTCTGATGGCACCCAGGGAAGTCCTCACAGGCAACGATGA; GGTCATCGGGCAGGTCCTGAGCACACTCAAGTCCGAAGATGTCCCATACACAGCGGCCCT; CACAGCGGTCCGCCCTTCCAGGTGGCCCGTGATGTANCCGTGGTGGCCGGAGGGCTAGGT; CGCCAGCTGCTACAAAACAGCCAGATCACCTGTGATCATNCTCCTGTGANTACATGACAC; GCTCCCNNGGATCTGNTCTGGCCCAAACTTCTCTGTGCGTACAGGACANTGGNNAGACTG; ACTCCTACTTTTGGGGGCAGAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_001183
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001183.3, NP_001174.2
• RefSeq Size: 2055 bp
• RefSeq ORF: 1413 bp
• Locus ID: 537
• UniProt ID: Q15904
• Cytogenetics: Xq28
• Protein Families: Transmembrane
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Summary: This gene encodes a component of a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. This protein may also play a role in early development. [provided by RefSeq, Aug 2013]