Cleavage stimulation factor 2 (CSTF2) (NM_001325) Human Untagged Clone

SKU SC119306

CSTF2 (untagged)-Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Cleavage stimulation factor 2
• Synonyms: CstF-64
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119306 sequence for NM_001325 edited (data generated by NextGen Sequencing); ATGGCGGGTTTGACTGTGAGAGACCCAGCGGTGGATCGTTCTCTACGTTCTGTGTTCGTG; GGGAACATTCCTTATGAAGCTACTGAAGAGCAGTTGAAGGACATCTTTTCTGAGGTTGGA; CCTGTTGTTAGTTTCAGATTGGTATACGATAGAGAGACAGGAAAGCCAAAGGGTTATGGC; TTCTGTGAATACCAAGACCAAGAGACAGCACTTAGTGCCATGCGGAACCTGAATGGGCGC; GAATTCAGTGGGAGAGCACTTCGAGTGGACAATGCTGCCAGTGAAAAGAACAAAGAAGAG; CTGAAGAGCCTTGGCACTGGTGCCCCTGTCATTGAGTCACCTTATGGAGAGACCATCAGT; CCTGAGGATGCCCCTGAGTCCATTAGCAAAGCAGTTGCCAGCCTTCCACCAGAGCAGATG; TTTGAGCTGATGAAACAAATGAAGCTCTGTGTCCAGAATAGTCCCCAGGAGGCACGGAAC; ATGTTACTTCAGAACCCTCAACTGGCTTATGCTTTGCTGCAAGCACAGGTAGTGATGAGA; ATTGTGGATCCGGAAATTGCCCTGAAAATTCTGCATCGCCAGACAAATATCCCAACGCTG; ATTGCAGGCAACCCTCAGCCAGTCCATGGTGCTGGGCCTGGCTCAGGATCCAATGTGTCA; ATGAACCAGCAGAATCCTCAGGCCCCTCAGGCCCAGTCTTTGGGTGGAATGCATGTCAAT; GGCGCACCTCCTCTGATGCAAGCTTCTATGCAGGGTGGAGTTCCAGCACCAGGGCAAATG; CCAGCTGCTGTCACAGGACCTGGCCCTGGTTCCTTAGCTCCTGGAGGAGGAATGCAGGCT; CAGGTTGGAATGCCAGGAAGTGGACCAGTGTCCATGGAACGGGGGCAAGTGCCGATGCAA; GACCCCAGAGCAGCTATGCAGCGGGGATCCTTGCCTGCGAATGTCCCAACCCCTCGAGGC; TTGTTAGGAGATGCTCCGAATGATCCACGGGGAGGCACTTTACTTTCTGTAACTGGAGAG; GTAGAGCCTAGAGGTTACTTGGGACCACCTCATCAGGGTCCACCCATGCACCATGTCCCT; GGCCATGAGAGCCGAGGACCACCCCCACATGAACTGAGGGGAGGGCCATTACCCGAGCCC; AGACCTCTAATGGCAGAACCAAGAGGACCCATGCTAGATCAGAGGGGTCCACCCTTGGAT; GGCAGAGGTGGAAGGGATCCCCGAGGAATAGATGCACGAGGGATGGAGGCCCGAGCCATG; GAGGCAAGAGGGTTAGATGCCAGAGGATTAGAGGCCCGTGCAATGGAGGCCCGTGCGATG; GAAGCTCGTGCAATGGAGGCCCGAGCGATGGAGGCCCGTGCAATGGAAGTCCGAGGGATG; GAGGCCAGAGGCATGGATACCAGAGGCCCAGTGCCTGGCCCCAGAGGACCTATACCTAGT; GGAATGCAGGGTCCCAGTCCAATTAACATGGGGGCGGTTGTCCCCCAGGGATCCAGACAG; GTCCCAGTCATGCAGGGAACAGGAATGCAAGGAGCAAGTATACAGGGTGGAAGCCAGCCT; GGCGGCTTTAGTCCCGGGCAGAACCAAGTCACTCCACAGGATCATGAGAAGGCTGCTTTG; ATTATGCAGGTTCTACAACTGACTGCAGACCAGATTGCCATGTTGCCTCCTGAGCAAAGG; CAGAGTATCCTGATTTTAAAGGAACAAATACAGAAATCCACTGGAGCACCTTGA; ; Clone variation with respect to NM_001325.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001325 unedited; GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAAGCCGACTCAACAG; AGCTATGGCGGGTTTGACTGTGAGAGACCCAGCGGTGGATCGTTCTCTACGTTCTGTGTT; CGTGGGGAACATTCCTTATGAAGCTACTGAAGAGCAGTTGAAGGACATCTTTTCTGAGGT; TGGACCTGTTGTTAGTTTCAGATTGGTATACGATAGAGAGACAGGAAAGCCAAAGGGTTA; TGGCTTCTGTGAATACCAAGACCAAGAGACAGCACTTAGTGCCATGCGGAACCTGAATGG; GCGCGAATTCAGTGGGAGAGCACTTCGAGTGGACAATGCTGCCAGTGAAAAGAACAAAGA; AGAGCTGAAGAGCCTTGGCACTGGTGCCCCTGTCATTGAGTCACCTTATGGAGAGACCAT; CAGTCCTGAGGATGCCCCTGAGTCCATTAGCAAAGCAGTTGCCAGCCTTCCACCAGAGCA; GATGTTTGAGCTGATGAAACAAATGAAGCTCTGTGTCCAGAATAGTCCCCAGGAGGCACG; GAACATGTTACTTCAGAACCCTCAACTGGCTTATGCTTTGCTGCAAGCACAGGTAGTGAT; GAGAATTGTGGATCCGGAAATTGCCCTGAAAATTCTGCATCGCCAGACAAATATCCCAAC; GCTGATTGCAGGCAACCCTCAGCCAGTCCATGGTGCTGGGCCTGGCTCANGATCCCATGT; GTCAATGAACCAGCAGAATCCTTCAGGCCCCTCAGCCCAGTCTTTGGGNTGGAATGCATG; TCATGGGCGCACCTNCTCTGNATGCAGCTTTATGCAGGGGTGGAGNTCCAGCACAGGGCA; ATGGCAGCTGCTGTACAGGACCTGGNCCCTGGTNCTTTACTNCTGNNAGAGAAATGCAGC; T;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001325 unedited; CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTGTTCAAAGTTTATTTCAAGTCACAG; AAAATATACAAACTAAGACAATAAAATGAAGTGATTTTGTTCTGAGAGAAGGTGATGGGA; GTCCTCCACCTGGCACTGATTTGAGTCATTCAAGGGTTAAGATGCAAGTCATCTTTTTTC; AATATTTCAACAAAATTATAGAATTTCCAGATTTCTTGCCAGGTATTTTTGAAAACCTAT; CAAGGTGCTCCAGTGGATTTCTGTATTTGTTCCTTTAAAATCAAGATACTCTGCCTTTGC; TCAAGAGGCAACATGGCAATCTGGTCTGCAGTCAGTTGTAGAACCTGCATAATCAAAGCA; GCCTTCTCATGATCCTGTGGAGTGACTTGGTTCTGCCCGGGACTAAAGCCGCCAGGCTGG; CTTCCACCCTGTATACTTGCTCCTTGCATTCCTGTTCCCTGCATGACTGGGACCTGTCTG; GATCCCTGGGGGACAACCGCCCCCATGTTAATTGGACTGGGACCCTGCATTCCACTAAGT; ATAGGTCCTCTGGGGCCAGGCACTGAGCCTCTGGTATCCATGCCTCTGGCCTCCATCCCT; CGGACTTNCATTGCACGGGCCTCCATCGCTCGGGCCTCCATTGCACGAGCTTCCATCGCA; CGGGCCTCCATAGCACGGGCCTCTAATCCTCTGGCATCTACCCTCTTGCCTCCATGGCTC; GGGCCTCCTCCCTCGTGCATCTATTCCTCGGGGATCCTTCCACCTCTGCCATCCAGGTTG; GACCCCTCTGATCANCATGGTCCTCCTGGTCCTGCATANAAGGCTGGGCTCGGTATGACC; CTCCCTCATTCATGTGGGGAGGCCCTGGCTCTCATGCCAGGACATGGGCATGGTGGACCT; GATGAGGGGGCCCAGTAACCCTAGGCTTACTCCTCGTACCAAAGAAAGGGCTCCCCGGAA; TATCCGGACATTTCTACAACCCGAGGGTTGAACTCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_001325
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001325.1, NP_001316.1
• RefSeq Size: 1978 bp
• RefSeq ORF: 1734 bp
• Locus ID: 1478
• UniProt ID: P33240
• Cytogenetics: Xq22.1
• Domains: RRM
• Summary: This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.