DNase II (DNASE2) (NM_001375) Human Untagged Clone

SKU SC119284

DNASE2 (untagged)-Human deoxyribonuclease II, lysosomal (DNASE2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DNase II
• Synonyms: DNASE2A; DNL; DNL2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119284 sequence for NM_001375 edited (data generated by NextGen Sequencing); ATGATCCCGCTGCTGCTGGCAGCGCTGCTGTGCGTCCCCGCCGGGGCCCTGACCTGCTAC; GGGGACTCCGGGCAGCCTGTAGACTGGTTCGTGGTCTACAAGCTGCCAGCTCTTAGAGGG; TCCGGGGAGGCGGCGCAGAGAGGGCTGCAGTACAAGTATCTGGACGAGAGCTCCGGAGGC; TGGCGGGACGGCAGGGCACTCATCAACAGCCCGGAGGGGGCCGTGGGCCGAAGCCTGCAG; CCGCTGTACCGGAGCAACACCAGCCAGCTCGCCTTCCTGCTCTACAATGACCAACCGCCT; CAACCCAGCAAGGCTCAGGACTCTTCCATGCGTGGGCACACGAAGGGTGTCCTGCTCCTT; GACCACGATGGGGGCTTCTGGCTGGTCCACAGTGTACCTAACTTCCCTCCACCGGCCTCC; TCTGCTGCATACAGCTGGCCTCATAGCGCCTGTACCTACGGGCAGACCCTGCTCTGTGTG; TCTTTTCCCTTCGCTCAGTTCTCGAAGATGGGCAAGCAGCTGACCTACACCTACCCCTGG; GTCTATAACTACCAGCTGGAAGGGATCTTTGCCCAGGAATTCCCCGACTTGGAGAATGTG; GTCAAGGGCCACCACGTTAGCCAAGAACCCTGGAACAGCAGCATCACACTCACATCCCAG; GCCGGGGCTGTTTTCCAGAGCTTTGCCAAGTTCAGCAAATTTGGAGATGACCTGTACTCC; GGCTGGTTGGCAGCAGCCCTTGGTACCAACCTGCAGGTCCAGTTCTGGCACAAAACTGTA; GGCATCCTGCCCTCTAACTGCTCGGATATCTGGCAGGTTCTGAATGTGAACCAGATAGCT; TTCCCTGGACCAGCCGGCCCAAGCTTCAACAGCACAGAGGACCACTCCAAATGGTGCGTG; TCCCCAAAAGGGCCCTGGACCTGCGTGGGTGACATGAATCGGAACCAGGGAGAGGAGCAA; CGGGGTGGGGGCACACTGTGTGCCCAGCTGCCAGCCCTCTGGAAAGCCTTCCAGCCGCTG; GTGAAGAACTACCAGCCCTGTAATGGCATGGCCAGGAAGCCCAGCAGAGCTTATAAGATC; TAA; ; Clone variation with respect to NM_001375.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001375 unedited; TAGATTTGTATACGACTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGATGTAACC; CAGCGCCCCGCAGTCCCGACACAGATTCCTGGATCTCAGCCCCATAGCAGCTATGATCCC; GCTGCTGCTGGCAGCGCTGCTGTGCGTCCCCGCCGGGGCCCTGACCTGCTACGGGGACTC; CGGGCAGCCTGTAGACTGGTTCGTGGTCTACAAGCTGCCAGCTCTTAGAGGGTCCGGGGA; GGCGGCGCAGAGAGGGCTGCAGTACAAGTATCTGGACGAGAGCTCCGGAGGCTGGCGGGA; CGGCAGGGCACTCATCAACAGCCCGGAGGGGGCCGTGGGCCGAAGCCTGCAGCCGCTGTA; CCGGAGCAACACCAGCCAGCTCGCCTTCCTGCTCTACAATGACCAACCGCCTCAACCCAG; CAAGGCTCAGGACTCTTCCATGCGTGGGCACACGAAGGGTGTCCTGCTCCTTGACCACGA; TGGGGGCTTCTGGCTGGTCCACAGTGTACCTAACTTCCCTCCACCGGCCTCCTCTGCTGC; ATACAGCTGGCCTCATAGCGCCTGTACCTACGGGCAGACCCTGCTCTGTGTGTCTTTTCC; CTTCGCTCAGTTCTCGAAGATGGGCAAGCAGCTGACCTACACCTACCCCTGGGTCTATAA; CTACCAGCTGGAAGGGATCTTTGCCCAGGAATTCCCCGACTTGGAGAATGTGGTCAAGGG; CCACCACGTTAGCCAAGAACCCTGGAACAGCAGCATCACACTCACATCCCAGCCCCGGGC; TGTTTTCCAGAGCTCTGCCAAGTTCAGCAAATTTGGAGATGACCTGTACTCCGGCTGGTT; GGCAGCAGCCCTTGGTCCAACCTGCAGGTCCAGTTCCTGCACAAAACTGTTAGCATCCTG; CCCTCTAACTGCTCGNATATTTGGCAGGTTCTGAATGTGGACCCGCATACCTTCCCTGTA; CCAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001375 unedited; CTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGATTTTTTATTTTTTATTTTTTTGACACAG; AGTCTCATTCTGTCACCCAGGCTGAAGTGCCGTGGTGTTAGCTCAGCTCATGACAACCTC; TGCCTCCCGAGTTCAAGCGATTCTCCTGCCTTACCCTCCCGAGGAGCTAGGACCACAGAC; GCGTGCCACCATGCCTGGCTAATCCTTAAACATTCTTAATGGATATGGGGTTTCACCATG; TTGGCCAGGCTGGCCTCGAACTCCTGACCTCAAGTGATCTGCCCATCTAAGACTCCCAAA; GTGCTGGGGCTACAGATGTGAGCCACTGCACCCACCCGTACACCGACCACCCTTTTTTTT; TGAAACAGAGCCTTGCTCTGTGACCTCACGCTGGCGCCCCACATAGCCCACTGTCACCAT; GATTCCCACTGGCCCAACCCATCCTCTGGCTTCACTGCGCCGGTACCCACAGGCCTTTAC; CACCCGCGCCTGCTTCCCTCTCTTCCAACTCCATCTCCACATGTGGCCTCCCTTTGCAAC; CCCCGCCTGTCCCCCCATCCCCTGCCTTCTAAGTGCACCCTTACCTCCTTCGACTTCGCC; AAGTCGCATGGAATACCATACCCGACCGCCTGCCCCTTGCCCCCAATGGCCTCCAACATT; ACTAACCCCTGCTGGGCCTACCCGGCCCATTCCATCACAGGGCTTGCCAACTCCCCCAAC; CCCCGTCCGTAACGATTATATTAAGGGCTCGCCCTCCGGGCCCCACAGTTGTGCCACACC; TCCCCACCACTATACATTCTTGCCACACCCCCCTGCGACACCCCCCCGTCTCGCGGCGAC; TTTTTCGGGACCCTCAACCCCTCTCTGCGTCGATTATACTCGCGCCTATATTAACCTTGG; CTTCATTTGACCACACCGCGCAANTGTGCTTGCCATATTTATCACGCCTTCCGCCTTTCC; CCTTCACCCCATTACGGCGACGCTCAATATAATACAGCGCTCATACACCCCGCATTCTCA; TCGCTNGTCCCCATGCGCTGCCGCACATTACCGCCCGTCCCGTGCCTCCCACTCCTCTAG; TCACGCCTCGCTCTANTCGCTNACCACACACCCACTCACGTTGTCGTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001375
• Insert Size: 1790 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001375.2, NP_001366.1
• RefSeq Size: 2011 bp
• RefSeq ORF: 1083 bp
• Locus ID: 1777
• UniProt ID: O00115
• Cytogenetics: 19p13.13
• Domains: DNase_II
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• Summary: This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. [provided by RefSeq, Jul 2008]