DNase II (DNASE2) (NM_001375) Human Untagged Clone

CAT#: SC119284

DNASE2 (untagged)-Human deoxyribonuclease II, lysosomal (DNASE2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNase II
• Synonyms: DNASE2A; DNL; DNL2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119284 sequence for NM_001375 edited (data generated by NextGen Sequencing)
  ATGATCCCGCTGCTGCTGGCAGCGCTGCTGTGCGTCCCCGCCGGGGCCCTGACCTGCTAC
  GGGGACTCCGGGCAGCCTGTAGACTGGTTCGTGGTCTACAAGCTGCCAGCTCTTAGAGGG
  TCCGGGGAGGCGGCGCAGAGAGGGCTGCAGTACAAGTATCTGGACGAGAGCTCCGGAGGC
  TGGCGGGACGGCAGGGCACTCATCAACAGCCCGGAGGGGGCCGTGGGCCGAAGCCTGCAG
  CCGCTGTACCGGAGCAACACCAGCCAGCTCGCCTTCCTGCTCTACAATGACCAACCGCCT
  CAACCCAGCAAGGCTCAGGACTCTTCCATGCGTGGGCACACGAAGGGTGTCCTGCTCCTT
  GACCACGATGGGGGCTTCTGGCTGGTCCACAGTGTACCTAACTTCCCTCCACCGGCCTCC
  TCTGCTGCATACAGCTGGCCTCATAGCGCCTGTACCTACGGGCAGACCCTGCTCTGTGTG
  TCTTTTCCCTTCGCTCAGTTCTCGAAGATGGGCAAGCAGCTGACCTACACCTACCCCTGG
  GTCTATAACTACCAGCTGGAAGGGATCTTTGCCCAGGAATTCCCCGACTTGGAGAATGTG
  GTCAAGGGCCACCACGTTAGCCAAGAACCCTGGAACAGCAGCATCACACTCACATCCCAG
  GCCGGGGCTGTTTTCCAGAGCTTTGCCAAGTTCAGCAAATTTGGAGATGACCTGTACTCC
  GGCTGGTTGGCAGCAGCCCTTGGTACCAACCTGCAGGTCCAGTTCTGGCACAAAACTGTA
  GGCATCCTGCCCTCTAACTGCTCGGATATCTGGCAGGTTCTGAATGTGAACCAGATAGCT
  TTCCCTGGACCAGCCGGCCCAAGCTTCAACAGCACAGAGGACCACTCCAAATGGTGCGTG
  TCCCCAAAAGGGCCCTGGACCTGCGTGGGTGACATGAATCGGAACCAGGGAGAGGAGCAA
  CGGGGTGGGGGCACACTGTGTGCCCAGCTGCCAGCCCTCTGGAAAGCCTTCCAGCCGCTG
  GTGAAGAACTACCAGCCCTGTAATGGCATGGCCAGGAAGCCCAGCAGAGCTTATAAGATC
  TAA
  
  Clone variation with respect to NM_001375.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001375 unedited
  TAGATTTGTATACGACTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGATGTAACC
  CAGCGCCCCGCAGTCCCGACACAGATTCCTGGATCTCAGCCCCATAGCAGCTATGATCCC
  GCTGCTGCTGGCAGCGCTGCTGTGCGTCCCCGCCGGGGCCCTGACCTGCTACGGGGACTC
  CGGGCAGCCTGTAGACTGGTTCGTGGTCTACAAGCTGCCAGCTCTTAGAGGGTCCGGGGA
  GGCGGCGCAGAGAGGGCTGCAGTACAAGTATCTGGACGAGAGCTCCGGAGGCTGGCGGGA
  CGGCAGGGCACTCATCAACAGCCCGGAGGGGGCCGTGGGCCGAAGCCTGCAGCCGCTGTA
  CCGGAGCAACACCAGCCAGCTCGCCTTCCTGCTCTACAATGACCAACCGCCTCAACCCAG
  CAAGGCTCAGGACTCTTCCATGCGTGGGCACACGAAGGGTGTCCTGCTCCTTGACCACGA
  TGGGGGCTTCTGGCTGGTCCACAGTGTACCTAACTTCCCTCCACCGGCCTCCTCTGCTGC
  ATACAGCTGGCCTCATAGCGCCTGTACCTACGGGCAGACCCTGCTCTGTGTGTCTTTTCC
  CTTCGCTCAGTTCTCGAAGATGGGCAAGCAGCTGACCTACACCTACCCCTGGGTCTATAA
  CTACCAGCTGGAAGGGATCTTTGCCCAGGAATTCCCCGACTTGGAGAATGTGGTCAAGGG
  CCACCACGTTAGCCAAGAACCCTGGAACAGCAGCATCACACTCACATCCCAGCCCCGGGC
  TGTTTTCCAGAGCTCTGCCAAGTTCAGCAAATTTGGAGATGACCTGTACTCCGGCTGGTT
  GGCAGCAGCCCTTGGTCCAACCTGCAGGTCCAGTTCCTGCACAAAACTGTTAGCATCCTG
  CCCTCTAACTGCTCGNATATTTGGCAGGTTCTGAATGTGGACCCGCATACCTTCCCTGTA
  CCAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001375 unedited
  CTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGATTTTTTATTTTTTATTTTTTTGACACAG
  AGTCTCATTCTGTCACCCAGGCTGAAGTGCCGTGGTGTTAGCTCAGCTCATGACAACCTC
  TGCCTCCCGAGTTCAAGCGATTCTCCTGCCTTACCCTCCCGAGGAGCTAGGACCACAGAC
  GCGTGCCACCATGCCTGGCTAATCCTTAAACATTCTTAATGGATATGGGGTTTCACCATG
  TTGGCCAGGCTGGCCTCGAACTCCTGACCTCAAGTGATCTGCCCATCTAAGACTCCCAAA
  GTGCTGGGGCTACAGATGTGAGCCACTGCACCCACCCGTACACCGACCACCCTTTTTTTT
  TGAAACAGAGCCTTGCTCTGTGACCTCACGCTGGCGCCCCACATAGCCCACTGTCACCAT
  GATTCCCACTGGCCCAACCCATCCTCTGGCTTCACTGCGCCGGTACCCACAGGCCTTTAC
  CACCCGCGCCTGCTTCCCTCTCTTCCAACTCCATCTCCACATGTGGCCTCCCTTTGCAAC
  CCCCGCCTGTCCCCCCATCCCCTGCCTTCTAAGTGCACCCTTACCTCCTTCGACTTCGCC
  AAGTCGCATGGAATACCATACCCGACCGCCTGCCCCTTGCCCCCAATGGCCTCCAACATT
  ACTAACCCCTGCTGGGCCTACCCGGCCCATTCCATCACAGGGCTTGCCAACTCCCCCAAC
  CCCCGTCCGTAACGATTATATTAAGGGCTCGCCCTCCGGGCCCCACAGTTGTGCCACACC
  TCCCCACCACTATACATTCTTGCCACACCCCCCTGCGACACCCCCCCGTCTCGCGGCGAC
  TTTTTCGGGACCCTCAACCCCTCTCTGCGTCGATTATACTCGCGCCTATATTAACCTTGG
  CTTCATTTGACCACACCGCGCAANTGTGCTTGCCATATTTATCACGCCTTCCGCCTTTCC
  CCTTCACCCCATTACGGCGACGCTCAATATAATACAGCGCTCATACACCCCGCATTCTCA
  TCGCTNGTCCCCATGCGCTGCCGCACATTACCGCCCGTCCCGTGCCTCCCACTCCTCTAG
  TCACGCCTCGCTCTANTCGCTNACCACACACCCACTCACGTTGTCGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001375
• Insert Size: 1790 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001375.2, NP_001366.1
• RefSeq Size: 2011 bp
• RefSeq ORF: 1083 bp
• Locus ID: 1777
• UniProt ID: O00115
• Cytogenetics: 19p13.13
• Domains: DNase_II
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• Gene Summary: This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. [provided by RefSeq, Jul 2008]