Non Neuronal Enolase (ENO1) (NM_001428) Human Untagged Clone

SKU SC119247

ENO1 (untagged)-Human enolase 1, (alpha) (ENO1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Non Neuronal Enolase
• Synonyms: ENO1L1; HEL-S-17; MPB1; NNE; PPH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001428, the custom clone sequence may differ by one or more nucleotides; ; ; ATGTCTATTCTCAAGATCCATGCCAGGGAGATCTTTGACTCTCGCGGGAATCCCACTGTTGAGGTTGATC; TCTTCACCTCAAAAGGTCTCTTCAGAGCTGCTGTGCCCAGTGGTGCTTCAACTGGTATCTATGAGGCCCT; AGAGCTCCGGGACAATGATAAGACTCGCTATATGGGGAAGGGTGTCTCAAAGGCTGTTGAGCACATCAAT; AAAACTATTGCGCCTGCCCTGGTTAGCAAGAAACTGAACGTCACAGAACAAGAGAAGATTGACAAACTGA; TGATCGAGATGGATGGAACAGAAAATAAATCTAAGTTTGGTGCGAACGCCATTCTGGGGGTGTCCCTTGC; CGTCTGCAAAGCTGGTGCCGTTGAGAAGGGGGTCCCCCTGTACCGCCACATCGCTGACTTGGCTGGCAAC; TCTGAAGTCATCCTGCCAGTCCCGGCGTTCAATGTCATCAATGGCGGTTCTCATGCTGGCAACAAGCTGG; CCATGCAGGAGTTCATGATCCTCCCAGTCGGTGCAGCAAACTTCAGGGAAGCCATGCGCATTGGAGCAGA; GGTTTACCACAACCTGAAGAATGTCATCAAGGAGAAATATGGGAAAGATGCCACCAATGTGGGGGATGAA; GGCGGGTTTGCTCCCAACATCCTGGAGAATAAAGAAGGCCTGGAGCTGCTGAAGACTGCTATTGGGAAAG; CTGGCTACACTGATAAGGTGGTCATCGGCATGGACGTAGCGGCCTCCGAGTTCTTCAGGTCTGGGAAGTA; TGACCTGGACTTCAAGTCTCCCGATGACCCCAGCAGGTACATCTCGCCTGACCAGCTGGCTGACCTGTAC; AAGTCCTTCATCAAGGACTACCCAGTGGTGTCTATCGAAGATCCCTTTGACCAGGATGACTGGGGAGCTT; GGCAGAAGTTCACAGCCAGTGCAGGAATCCAGGTAGTGGGGGATGATCTCACAGTGACCAACCCAAAGAG; GATCGCCAAGGCCGTGAACGAGAAGTCCTGCAACTGCCTCCTGCTCAAAGTCAACCAGATTGGCTCCGTG; ACCGAGTCTCTTCAGGCGTGCAAGCTGGCCCAGGCCAATGGTTGGGGCGTCATGGTGTCTCATCGTTCGG; GGGAGACTGAAGATACCTTCATCGCTGACCTGGTTGTGGGGCTGTGCACTGGGCAGATCAAGACTGGTGC; CCCTTGCCGATCTGAGCGCTTGGCCAAGTACAACCAGCTCCTCAGAATTGAAGAGGAGCTGGGCAGCAAG; GCTAAGTTTGCCGGCAGGAACTTCAGAAACCCCTTGGCCAAGTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001428 unedited; ATACGACTCACTTAGGGCGGCCGCGACTTCGGCACGAGGCGCGGAAAGTATCTGTGGGTA; CCCGGAGCCGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTCCGCT; TTCCTCTCCTAGGCGACGAGACCCAGTGGCTAGAAGTTCACCATGTCTCTTCTCAAGCTC; CATGCCAGGGAGCTCTTTGACTCTCGCGGGAATCCCACTGTTGAGGTTGATCTCTTCACC; TCAAAAGGTCTCTTCAGAGCTGCTGTGCCCAGTGGTGCTTCAACTGGTATCTATGAGGCC; CTAGAGCTCCGGGACAATGATAAGACTCGCTATATGGGGAAGGGTGTCTCAAAGGCTGTT; GAGCACATCAATAAAACTATTGCGCCTGCCCTGGTTAGCAAGAAACTGAACGTCACAGAA; CAAGAGAAGATTGACAAACTGATGATCGAGATGGATGGAACAGAAAATAAATCTAAGTTT; GGTGCGAACGCCATTCTGGGGNGTGTCCCTTGCCGTCTGCAAAGCTGGTGCCGTTGAGAA; GGGGGTCCCCCTGTACCGCCACATCGCTGACTTGGCTGGCAACTCTGAAGTCATCCTGCC; AGTCCCGGCGTTCAATGTCATCAATGGCGGTTCTCATGCTGGCAACAAGCTGGCCATGCA; GGAGTTCATGATCCTNCCAGTCGGTGCAGCAAACTTCAGGGAAGCCATGCGCATTGGAGC; AGAGGTTTACCACAACCTGAAGAATGTCATCAAGGAGAAAATGGGAANAGAGCCACCAAT; GTGGGGGATGAAGGGCGGGTTTGCTCCCAACATNNCTGAGATAAAGAANGNCTGNNACTG; CTGAAGACTGCTATTGGGAAAACTGGCTAACTGAATAAGGTGGCAATCGCATGGACGTAC; CGCCTTCGATTTTTTTAGGTTGGGGAATTAGACCTTGATTTAAACTA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001428 unedited; NNTTTTTACTCTGGCCCGGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTCTCAGGGGCCACTGAGGCTTTTTATTTTGAGCACA; AAACCACCGGGGATCTACCCTGGGGCCACCCCGGAAATGACACGAGGCTCACATGACTCT; AAACACTTGGGGGAAAGGGAGGCGAAAAAAACAATGACTTGGGCCAATTACACAACTGCA; AAGCTAGAGCTGCCAACAGGGCTCCAGGGAGCTTGGCTTCTGTAAAAGTTCTAAGGAAGC; GGTACAAACTCCACGGGGGGGGGGCGCTAACTAGCAGGGACCCCTGCAAGTGTTGGTCGG; GGGCCTCAAGCTGCCTGAGCTGACACGAGGGGAGGGGTCTGTGTACCCAACAGGTGACCG; AAGGGCTTGCCTGCCCACAGTTTACTTGGCCAAGGGGTTTCTGAAGTTCCTGCCGGCAAA; CTTACCCTTGCTGCCCAGCTCCTTTTCAATTCTGAGGAGCTGGTTGTACTTGGCCAAGCG; CTCAGATCGGCAAGGGGCACCAGTCTTGATCTGCCCAGTGCACAGCCCCACAACCAGGTC; AGCGATGAAGGTATCTTCAGTCTCCCCCGAACGATGAGACACCATGACGCCCCAACCATT; GGCCTGGGCCAGCTTGCACGCCTGAAGAGACTCGGCCACGAAGCCAATCTGTTTGACTTT; GACCAGGAAGCCATTGCAGGACTTTTCGTTCACGGCCTTGAAGATCCTTTTTGGTTTGGC; ACTGGGAGATCATCCCCCATACCTGAATTCCGCCCTGGCTGGAACCTTTGCCAAGCTCCC; AATATCCTGGAAAAGGAATCCTTAATAACCCCACTGGTAACCCTGGAAGAGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001428
• Insert Size: 1770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001428.2, NP_001419.1
• RefSeq Size: 1812 bp
• RefSeq ORF: 1305 bp
• Locus ID: 2023
• UniProt ID: P06733
• Cytogenetics: 1p36.23
• Domains: enolase
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation
• Summary: This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]; Transcript Variant: This variant (1) encodes isoform (1), which is localized to the cytosol, and has alpha-enolase activity. It has been reported that the monomeric form of this isoform functions as a structural lens protein (tau-crystallin), and the dimeric form as an enolase (PMID:2462567).