GTF3C2 (NM_001521) Human Untagged Clone

SKU SC119183

GTF3C2 (untagged)-Human general transcription factor IIIC, polypeptide 2, beta 110kDa (GTF3C2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: GTF3C2
• Synonyms: TFIIIC-BETA; TFIIIC110
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001521, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGATACCTGCGGGGTCGGCTATGTTGCCCTGGGGGAGGCCGGCCCCGTGGGGAACATGACTGTGGTAG; ACTCTCCTGGACAAGAGGTGCTAAATCAGCTTGATGTCAAGACCTCTTCAGAAATGACCAGTGCAGAGGC; TTCCGTAGAGATGTCATTACCTACCCCTTTGCCTGGATTTGAGGATTCTCCTGATCAGAGGAGGCTCCCT; CCAGAGCAGGAAAGCCTCTCCAGACTGGAACAGCCAGATCTTTCTTCAGAGATGTCAAAGGTCTCAAAGC; CTAGGGCCTCAAAGCCTGGCCGGAAGAGAGGTGGTAGGACACGAAAAGGCCCCAAAAGGCCCCAACAGCC; TAATCCTCCATCAGCCCCACTGGTTCCTGGTCTCTTAGATCAATCCAACCCTCTGTCCACCCCCATGCCT; AAGAAACGAGGTCGAAAGTCCAAGGCAGAGCTGCTGCTGCTGAAGTTGTCAAAAGACCTAGATCGGCCAG; AATCTCAATCTCCAAAGAGGCCCCCTGAGGACTTTGAGACCCCTTCTGGGGAACGACCCCGCCGAAGGGC; TGCCCAAGTGGCACTTCTGTATCTTCAGGAACTGGCTGAAGAGCTCTCAACAGCCCTGCCTGCCCCTGTG; TCCTGTCCTGAGGGCCCCAAGGTGAGCAGCCCCACCAAACCGAAGAAGATCCGGCAGCCAGCAGCCTGTC; CAGGTGGAGAAGAGGTGGATGGTGCTCCACGGGATGAAGACTTTTTTCTCCAGGTTGAGGCTGAAGATGT; GGAAGAAAGTGAGGGCCCAAGTGAGAGCTCATCTGAACCTGAGCCTGTAGTGCCCCGAAGCACCCCACGA; GGATCTACTTCAGGGAAACAGAAACCACACTGCCGAGGAATGGCTCCCAATGGCTTACCAAATCATATCA; TGGCTCCTGTTTGGAAGTGCCTCCATCTCACCAAGGACTTCCGAGAGCAGAAACATTCATACTGGGAGTT; TGCTGAGTGGATTCCTTTAGCCTGGAAGTGGCACTTGTTATCTGAGCTTGAGGCCGCTCCCTACCTGCCC; CAGGAGGAGAAGTCTCCATTGTTTTCTGTACAACGTGAAGGGCTACCTGAAGATGGCACCCTCTACCGAA; TAAACAGATTTAGCTCGATCACAGCACATCCAGAGCGCTGGGATGTGTCCTTCTTCACGGGGGGACCGCT; CTGGGCTCTGGACTGGTGCCCAGTGCCAGAGGGGGCAGGAGCCTCGCAATATGTGGCTCTTTTCTCCAGC; CCTGACATGAATGAGACACACCCACTGAGCCAGCTTCATTCGGGTCCTGGGCTGCTCCAGCTCTGGGGCC; TTGGGACCTTGCAGCAAGAAAGCTGTCCTGGCAACAGGGCCCACTTTGTCTATGGGATTGCTTGTGACAA; CGGCTGCATCTGGGACCTCAAGTTCTGCCCCAGTGGAGCATGGGAACTTCCAGGCACCCCTCGGAAGGCT; CCTCTCCTGCCCCGGTTGGGTCTCTTGGCTCTGGCCTGCTCAGACGGGAAAGTACTGCTATTCAGTCTAC; CCCATCCGGAGGCCCTGCTGGCTCAGCAACCCCCAGATGCAGTGAAGCCTGCCATATATAAGGTACAATG; TGTGGCAACTCTGCAGGTGGGGTCTATGCAAGCTACAGACCCCTCTGAGTGTGGTCAGTGCCTTAGCCTG; GCCTGGATGCCTACCAGGCCCCACCAACACCTAGCTGCTGGATATTATAATGGCATGGTGGTTTTCTGGA; ACCTTCCCACTAACTCACCCCTGCAGCGGATACGGCTCTCTGATGGCTCCTTAAAGCTCTACCCCTTCCA; GTGTTTCCTAGCCCATGACCAGGCTGTGCGTACCCTTCAATGGTGCAAAGCTAACAGCCATTTCCTTGTC; TCTGCGGGGAGTGACCGGAAAATCAAATTCTGGGACCTTCGACGTCCTTACGAACCCATAAACTCTATCA; AGCGCTTCTTGAGTACAGAACTGGCCTGGCTGCTTCCCTACAATGGTGTCACTGTGGCTCAGGACAACTG; CTATGCCTCTTATGGACTCTGTGGGATTCATTATATTGACGCTGGTTACCTTGGTTTCAAGGCCTACTTC; ACTGCTCCTCGAAAAGGCACCGTTTGGAGTCTTTCAGGATCCGACTGGCTTGGGACAATAGCTGCAGGAG; ATATATCCGGGGAGCTCATTGCTGCTATATTACCAGATATGGCACTGAATCCAATAAATGTCAAGCGACC; TGTAGAGCGAAGATTTCCTATATATAAAGCAGATCTGATACCGTATCAGGACAGTCCTGAAGGTCCAGAC; CATTCTTCTGCTTCATCTGGGGTCCCCAACCCTCCTAAGGCTCGAACTTACACTGAAACTGTCAACCATC; ACTACTTGCTCTTTCAAGACACAGATTTGGGTTCATTCCATGATCTGCTCCGTAGAGAACCAATGCTGCG; CATGCAGGAGGGAGAGGGGCATTCTCAACTCTGCCTGGACAGGCTGCAGCTGGAGGCTATTCATAAGGTA; CGTTTCAGCCCAAACCTGGACTCCTATGGATGGCTGGTATCTGGGGGGCAGTCAGGGCTGGTTCGAATCC; ATTTTGTCCGTGGACTCGCCTCCCCACTGGGCCACCGTATGCAGCTTGAAAGCCGAGCCCACTTCAATGC; TATGTTCCAACCATCCTCCCCCACTAGACGGCCTGGCTTCTCTCCAACCAGCCATCGCCTTCTGCCCACT; CCCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001521 unedited; AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCA; CGAGGCAGAATCCCCTGAAGAGCTTGTTAAAACACAAGGTGCTGGCCCCATCCAGAATTG; CTGATTCGGGGCAGCCATTTTGGGGGGTGCTGATGGATACCTGCGGGGTCGGCTATGTTG; CCCTGGGGGAGGCCGGCCCCGTGGGGAACATGACTGTGGTAGACTCTCCTGGACAAGAGG; TGCTAAATCAGCTTGATGTCAAGACCTCTTCAGAAATGACCAGTGCAGAGGCTTCCGTAG; AGATGTCATTACCTACCCCTTTGCCTGGATTTGAGGATTCTCCTGATCAGAGGAGGCTCC; CTCCAGAGCAGGAAAGCCTCTCCAGACTGGAACAGCCAGATCTTTCTTCAGAGATGTCAA; AGGTCTCAAAGCCTAGGGCCTCAAAGCCTGGCCGGAAGAGAGGTGGTAGGACACGAAAAG; GCCCCAAAAGGCCCCAACAGCCTAATCCTCCATCAGCCCCACTGGTTCCTGGTCTCTTAG; ATCAATCCAACCCTCTGTCCACCCCCATGCCTAAGAAACGAGGTCGAAAGTCCAAGGCAG; AGCTGCTGCTGCTGAAGTTGTCAAAAGACCTAGATCGGCCAGAATCTCAATCTCCAAAGA; GGCCCCCCTGAGACTNTGAGACCCNCTTCTGGGAACGANCCCCGCCGAAGGCTGCCCAAG; TGGCACTTCTGTATCTTCAGGAACTGGCTGAAGAGCTCTCAACAGCCCTGCCTGCCCCTG; TGTCCTGTCCTGAGGNNCCCCANGTGAGCAGCCCACCANACCGAGNAAGANCCGCAGCCA; GCAGCCTGTCCCAGTGGAGAAAGAGTGGGATGGNTGCTCACAGGATGAAGACTTTNTTCT; NCAGNTGAGGCTGAAGAGTGNAAAAAATGAGGCCCNAGTGAGACTCTCTGACT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001521 unedited; CGGCCGCATTCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAGTAACTGA; AGATATTTAATCTAGAGAAGATTTGGGAAACACATGATAGCTATGGTTAAATACTTAACA; GGGCAATCACAGGGAAGATGACTAGATTTCCTAACATCCATGAGTGAAATTTATAGAAGT; ATACTCTCTGACTTGATATAAAGGAAGATTTTAAAAAACATGACTGTTCAGGAGTGTTCA; AGTAGGGTCAGATGACCAGTGATTGGGAATACTTCGTAAGCAGGAGCAAGTAAGATCTGA; GCCACTGTTCTATCGGTAGGGGGTCTGTGGTATTCCTTGGTCAAAGAAGTACTCTAAGCA; ACTTCAGTCTCACGAATTACTATCACCCTCGTGGGCATACATGATGGTTACCCTAAAGAG; GAAGTTTCAGAAGGCAGTAATATTGGATCCTGGAATAGTCAGACAGGAGCCTTCATGCAG; ATACCCTTTTCAGTTCTCCATACACCCATTCACAAGTGGTCACAAAAACACCCAGTACCT; TTACTTGGCTTTACCCACTTAACAATATGCTCAATATGAGCAGTTTGACTCCCAAGCCTA; GGTCAGGCCTGAGTGTTTCTCATGAGCCATAGTCTTAGGCTGAGGAGCCTTGAGAGTTGG; TGGAGGGAGAGCCCCCCTGCCCGCAGGGGGCTGTGATCACAGAAGTCTGTAGAAAGAGTG; GGATCTAAGGAGTCCTGGGGAGGCAGGCCTAAAGGTCCAGGCCTGGCATGATCACTGTCC; AGGGAAGGCCCCCAGNTTCTATGGCTCTGTGCATAGGGGCCACTTTGNTCTTGACCGACT; TCACTTCAAGGATCTGGTGTGGGCCCAGGCTAGGNAGTGGGCANAAAGCGATGGGCTGGN; TGAGAAANCCAGCCGTCTACTGGGGAGGATGTTGGNACTACCATGAAGTGGCTCGCCTTC; AGCTGATACG;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001521
• Insert Size: 3600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001521.1, NP_001512.1
• RefSeq Size: 3976 bp
• RefSeq ORF: 2736 bp
• Locus ID: 2976
• UniProt ID: Q8WUA4
• Cytogenetics: 2p23.3
• Domains: WD40
• Protein Families: Transcription Factors
• Summary: Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 1 and 2 both encode the same isoform (b).