GRK2 (NM_001619) Human Untagged Clone

CAT#: SC119158

ADRBK1 (untagged)-Human adrenergic, beta, receptor kinase 1 (ADRBK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRK2
• Synonyms: ADRBK1; BARK1; BETA-ARK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001619 edited
  ATGGCGGACCTGGAGGCGGTGCTGGCCGACGTGAGCTACCTGATGGCCATGGAGAAGAGC
  AAGGCCACGCCGGCCGCGCGCGCCAGCAAGAAGATACTGCTGCCCGAGCCCAGCATCCGC
  AGTGTCATGCAGAAGTACCTGGAGGACCGGGGCGAGGTGACCTTTGAGAAGATCTTTTCC
  CAGAAGCTGGGGTACCTGCTCTTCCGAGACTTCTGCCTGAACCACCTGGAGGAGGCCAGG
  CCCTTGGTGGAATTCTATGAGGAGATCAAGAAGTACGAGAAGCTGGAGACGGAGGAGGAG
  CGTGTGGCCCGCAGCCGGGAGATCTTCGACTCATACATCATGAAGGAGCTGCTGGCCTGC
  TCGCATCCCTTCTCGAAGAGTGCCACTGAGCATGTCCAAGGCCACCTGGGGAAGAAGCAG
  GTGCCTCCGGATCTCTTCCAGCCATACATCGAAGAGATTTGTCAAAACCTCCGAGGGGAC
  GTGTTCCAGAAATTCATTGAGAGCGATAAGTTCACACGGTTTTGCCAGTGGAAGAATGTG
  GAGCTCAACATCCACCTGACCATGAATGACTTCAGCGTGCATCGCATCATTGGGCGCGGG
  GGCTTTGGCGAGGTCTATGGGTGCCGGAAGGCTGACACAGGCAAGATGTACGCCATGAAG
  TGCCTGGACAAAAAGCGCATCAAGATGAAGCAGGGGGAGACCCTGGCCCTGAACGAGCGC
  ATCATGCTCTCGCTCGTCAGCACTGGGGACTGCCCATTCATTGTCTGCATGTCATACGCG
  TTCCACACGCCAGACAAGCTCAGCTTCATCCTGGACCTCATGAACGGTGGGGACCTGCAC
  TACCACCTCTCCCAGCACGGGGTCTTCTCAGAGGCTGACATGCGCTTCTATGCGGCCGAG
  ATCATCCTGGGCCTGGAGCACATGCACAACCGCTTCGTGGTCTACCGGGACCTGAAGCCA
  GCCAACATCCTTCTGGACGAGCATGGCCACGTGCGGATCTCGGACCTGGGCCTGGCCTGT
  GACTTCTCCAAGAAGAAGCCCCATGCCAGCGTGGGCACCCACGGGTACATGGCTCCGGAG
  GTCCTGCAGAAGGGCGTGGCCTACGACAGCAGTGCCGACTGGTTCTCTCTGGGGTGCATG
  CTCTTCAAGTTGCTGCGGGGGCACAGCCCCTTCCGGCAGCACAAGACCAAAGACAAGCAT
  GAGATCGACCGCATGACGCTGACGATGGCCGTGGAGCTGCCCGACTCCTTCTCCCCTGAA
  CTACGCTCCCTGCTGGAGGGGTTGCTGCAGAGGGATGTCAACCGGAGATTGGGCTGCCTG
  GGCCGAGGGGCTCAGGAGGTGAAAGAGAGCCCCTTTTTCCGCTCCCTGGACTGGCAGATG
  GTCTTCTTGCAGAAGTACCCTCCCCCGCTGATCCCCCCACGAGGGGAGGTGAACGCGGCC
  GACGCCTTCGACATTGGCTCCTTCGATGAGGAGGACACAAAAGGAATCAAGTTACTGGAC
  AGTGATCAGGAGCTCTACCGCAACTTCCCCCTCACCATCTCGGAGCGGTGGCAGCAGGAG
  GTGGCAGAGACTGTCTTCGACACCATCAACGCTGAGACAGACCGGCTGGAGGCTCGCAAG
  AAAGCCAAGAACAAGCAGCTGGGCCATGAGGAAGACTACGCCCTGGGCAAGGACTGCATC
  ATGCATGGCTACATGTCCAAGATGGGCAACCCCTTCCTGACCCAGTGGCAGCGGCGGTAC
  TTCTACCTGTTCCCCAACCGCCTCGAGTGGCGGGGCGAGGGCGAGGCCCCGCAGAGCCTG
  CTGACCATGGAGGAGATCCAGTCGGTGGAGGAGACGCAGATCAAGGAGCGCAAGTGCCTG
  CTCCTCAAGATCCGCGGTGGGAAACAGTTCATTTTGCAGTGCGATAGCGACCCTGAGCTG
  GTGCAGTGGAAGAAGGAGCTGCGCGACGCCTACCGCGAGGCCCAGCAGCTGGTGCAGCGG
  GTGCCCAAGATGAAGAACAAGCCGCGCTCGCCCGTGGTGGAGCTGAGCAAGGTGCCGCTG
  GTCCAGCGCGGCAGTGCCAACGGCCTCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001619 unedited
  CTATAGGGGCGGCCGCGAATTCGCACGAGGCGGGGCCGGAGGCGGCGGCGGCTCCGGGGC
  GCGGGCGCGCGGGCGGCGGCGGCGGCGGCGCCCCGACTGCAGTCCCGGCGGGAGCGGAGC
  GCGAGCCGGGGCCGGGCCCGAGCCGGCGCCATGGGGCGGCGCCGCCTGTGAGCGGCGGCG
  AGCGGAGCCGCGGGCGCCGAGCAGGGCCAGGCGGGAGGCGTCGGCGCCCGAGGCCGAGCG
  AGCCGCGGCCGGGCCGGGCCGAGCGCCGAGCGAGCAGGAGCGGCGGCGGCGGCGGCGGCG
  GCGGGAGGAGGCAGCGCCGCCGCCAAGATGGCGGACCTGGAGGCGGTGCTGGCCGACGTG
  AGCTACCTGATGGCCATGGAGAAGAGCAAGGCCACGCCGGCCGCGCGCGCCAGCAAGAAG
  ATACTGCTGCCCGAGCCCAGCATCCGCAGTGTCATGCAGAAGTACCTGGAGGACCGGGGC
  GAGGTGACCTTTGAGAAGATCTTTTCCCAGAAGCTGGGGTACCTGCTCTTCCGAGACTTC
  TGCCTGAACCACCTGGAGGAGGCCANGCCCTTGGTGNGATTCTATGAGGAGATCAAGAAG
  TACCAAAAGCTGGAGACGGAGGAAGAGCGTGTGGCCCGCACCCGGAGATCTTCGACTCAT
  ACATCATGAAAGAGCTGCTGGCCTGCTCGCATCCCTTCTCGAGAGTGCCACTGAGCATGT
  CCACAGCCACCTGGGGGAGAAGCAGGTGCCTCCGGATTTTTTCAGCCTACATTGAAAAGA
  TTTGCCAAACCTCCGAGGGGACGGGTTCCGAACTTCATGGGAACAAAAGTTCCCCCGTTT
  TGCCGTGGAATAATGTGGAGCTCCACATCCCTGACCTGAATACTTAACGGCATCCATTAT
  TGGGCGGGGGCTTTGCGAGGCTTATGGGCCGGAGGTTCACACGCAATGTCCCCTGTAGCC
  TGACAAACGCTTAAATGAACGGGGGACCCTGGCTGAAACGATTGTTCTTTTCACCTGGAT
  CCTAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001619 unedited
  GGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTACTCTTTAAAACACATTCAAAA
  ATCGGCAAAGAAACGCCCCGGGGAATGAGTAGACGGGAGGGGAGGGTGCGCCAACCCAGC
  CCCGGGGGGGAGAAGGCGCGACACCACACAGGCAGCGCGACTGGCACGAGGGGGCATGCG
  GTGGGCGAGGCGGCGGCACTGACAGGACGTGGGCTGTGGGGCTGGCCAGCCTGGGCGGGG
  GTACACTGATGGGCCAGGATGGGGGGCACACTGATAGGCCCCAGCCAGCCTCCTTGCTGT
  GCTGCCCGCCCACCTGGCCGGCCCCGTCCTGGGCAGATGGGGTCGCACCCAGCCCAGCAT
  CAGCGAGACCTCAGGCCAGGCGAGGCCAGTATGGCCCAACCTGCAGGTGCCTTGCTGTGG
  TCCCTGAGCCCGGGTGCTGGGGGTTGGGGCAAGAGGGGACAGCAGGGAGGGTAGCAGCAC
  CTGCTGCCAATGCCACTCTCTCAGCCAAGCCCCGCAGTGTCAGGGAAGTGGGAGTGGAGG
  CAGCCAGTGACACAGAGGCTTTTCCCTGATAAGGGCACAGGAGCCCGAGTCACTGTGGGA
  GGCCTTGAGGGCCAGGGAGACAACGGGCCCCTCCTCTCGCCAACACTGTGGAGCAGGACA
  GTGCAAGGCCTGCCCCTCTGCACCAGGACGGGGCGCAGTTGGGAAAATCCAAGCCGGATC
  CCTGGGCTTGTGCCCTGCCCTTGGGGAAGGGGGGCCGGGCCCCCCNCTCACGCCCCCATT
  GGCACCCATCCGTATCCCCTTTCACACCCCTGTGGACACCACCTCGTTGTGCCACCCACA
  CACTGCACAACCGTATATCCCTCCACACCCACCCTTCTCCGCCCCCGTCATCCCAACGTC
  ACCCGGTACCGCCACATTCACCCCGCACTACATTGCTCGCCCCCGTGTACCCCCGTCAAC
  GCAGATTACCGCCGTCCCCACCTTACTTGACGCGTTCGACACCCCAATCCACCTCGCCTG
  AATACCACACTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001619
• Insert Size: 2070 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001619.2, NP_001610.1
• RefSeq Size: 3603 bp
• RefSeq ORF: 2070 bp
• Locus ID: 156
• UniProt ID: P25098
• Cytogenetics: 11q13.2
• Domains: RGS, pkinase, S_TK_X, TyrKc, PH, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• Gene Summary: This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017]