IP10 (CXCL10) (NM_001565) Human Untagged Clone

CAT#: SC119137

CXCL10 (untagged)-Human chemokine (C-X-C motif) ligand 10 (CXCL10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IP10
• Synonyms: C7; crg-2; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119137 sequence for NM_001565 edited (data generated by NextGen Sequencing)
  ATGAATCAAACTGCCATTCTGATTTGCTGCCTTATCTTTCTGACTCTAAGTGGCATTCAA
  GGAGTACCTCTCTCTAGAACTGTACGCTGTACCTGCATCAGCATTAGTAATCAACCTGTT
  AATCCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCCACGTGTT
  GAGATCATTGCTACAATGAAAAAGAAGGGTGAGAAGAGATGTCTGAATCCAGAATCGAAG
  GCCATCAAGAATTTACTGAAAGCAGTTAGCAAGGAAAGGTCTAAAAGATCTCCTTAA
  
  Clone variation with respect to NM_001565.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001565 unedited
  NTAAGGTTCCGTTTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGATATT
  CTGAGCCTACAGCAGAGGAACCTCCAGTCTCAGCACCATGAATCAAACTGCCATTCTGAT
  TTGCTGCCTTATCTTTCTGACTCTAAGTGGCATTCAAGGAGTACCTCTCTCTAGAACTGT
  ACGCTGTACCTGCATCAGCATTAGTAATCAACCTGTTAATCCAAGGTCTTTAGAAAAACT
  TGAAATTATTCCTGCAAGCCAATTTTGTCCACGTGTTGAGATCATTGCTACAATGAAAAA
  GAAGGGTGAGAAGAGATGTCTGAATCCAGAATCGAAGGCCATCAAGAATTTACTGAAAGC
  AGTTAGCAAGGAAAGGTCTAAAAGATCTCCTTAAAACCAGAGGGGAGCAAAATCGATGCA
  GTGCTTCCAAGGATGGACCACACAGAGGCTGCCTCTCCCATCACTTCCCTACATGGAGTA
  TATGTCAAGCCATAATTGTTCTTAGTTTGCAGTTACACTAAAAGGTGACCAATCATGGTC
  ACCAAATCAGCTGCTACTACTCCTGTAGGAAGATTAATGTTCATCATCCTAAGCTATTCA
  GTAATAACTCTACCCTGGCACTATAATGTAAGCTCTACTGAGGTGCTATGTTCTTAGTGG
  ATGTTCTGACCCTGCTTCAAATATTTCCCTCACCTTTCCCATCTTCCAAGGGTATAAGGA
  ATCTTTCTGCTTTGGGGTTTATCAGAATTCTCAGAATCTCAAATAACTAANAGGTATGCA
  ATCAAATCTGCTTTTTTAAGAATGCTCTTTACTTCATGGACTTNCACTGCCATCCCTCCA
  AGGGGCCCAAATTCTTTCAGTGGCTACCTACATACAATTCCAAACACATACAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001565 unedited
  CCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCTTAGTTATAATTACTTT
  ATTAACCTTTTGGTCTTTCAACATTTAGATAGTCTTTCTTAATATTTCCAGGAGAGTACC
  TCATTTTTATTTTGAAAACCATTCAGCACATTTATCTTATGTAACATGCAGAGCATATAT
  CTATCTGTATTTTTAAAATTTTCCTGTTACTCATTGATACATAGTACTTAATTACATGTT
  ATTCCATGTACACTGAAAACAATATAGGAAATATATACATCTAAGACTTCTACTTTGTAC
  AGTCTTTCATTAAATAAGAATACTTACACATACATTTTCAGATATTTCTACCTTCCTGTA
  TGTGTTTGGAATTGTATGTAGGTAGCCACTGAAAGAATTTGGGCCCCTTGGGAGGATGGC
  AGTGGAAGTCCATGAAGTAAAGAGCATTCTTTAAAAAGCAGATTTGATTGCATACCTTTT
  AGTTATTTGAGATTCTGAGAATTCTGATAAACCCCAAAGCAGAAAGATTCCTTATACCCT
  TGGAAGATGGGAAAGGTGAGGGAAATATTTGAAGCAGGGTCAGAACATNCACTAAGAACA
  TAGCCCCCTCAGTAGAAGCTTACATTATANGTGCCAGGGTTAGAGTTATTACTGAATAGC
  TTAGGACGATGAACATTAATCTTTCTACAGGAGTAGTAACCGCTGATTTGGGTGACCATG
  ATTGGGCCCCCTTTTAATGTACCTGCAAACTAAAACAATTTTGGCTTGACATACCCTCCC
  TGTCGGGAAATGATGGGAAAAGGCACCCTTTGTGTGGTCCTTCCCTGGAACCCCTGGCTC
  AATTTGGCCCCCCTCCGGTTTAAGGGAAACCTTTACACCTCTCCTGCTAACGGCTCCCAA
  AAATCTTGAAGCCCTTCAATTTGGATAAACATCCTTTATCCCCTTTTTGTATGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001565
• Insert Size: 1140 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001565.1, NP_001556.1
• RefSeq Size: 1172 bp
• RefSeq ORF: 297 bp
• Locus ID: 3627
• UniProt ID: P02778
• Cytogenetics: 4q21.1
• Domains: IL8
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway
• Gene Summary: This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. This gene may also be a key regulator of the 'cytokine storm' immune response to SARS-CoV-2 infection. [provided by RefSeq, Sep 2020]