IP10 (CXCL10) (NM_001565) Human Untagged Clone

SKU SC119137

CXCL10 (untagged)-Human chemokine (C-X-C motif) ligand 10 (CXCL10)

Product Data

• Type: Human Untagged Clone
• Target Symbol: IP10
• Synonyms: C7; crg-2; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119137 sequence for NM_001565 edited (data generated by NextGen Sequencing); ATGAATCAAACTGCCATTCTGATTTGCTGCCTTATCTTTCTGACTCTAAGTGGCATTCAA; GGAGTACCTCTCTCTAGAACTGTACGCTGTACCTGCATCAGCATTAGTAATCAACCTGTT; AATCCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCCACGTGTT; GAGATCATTGCTACAATGAAAAAGAAGGGTGAGAAGAGATGTCTGAATCCAGAATCGAAG; GCCATCAAGAATTTACTGAAAGCAGTTAGCAAGGAAAGGTCTAAAAGATCTCCTTAA; ; Clone variation with respect to NM_001565.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001565 unedited; NTAAGGTTCCGTTTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGATATT; CTGAGCCTACAGCAGAGGAACCTCCAGTCTCAGCACCATGAATCAAACTGCCATTCTGAT; TTGCTGCCTTATCTTTCTGACTCTAAGTGGCATTCAAGGAGTACCTCTCTCTAGAACTGT; ACGCTGTACCTGCATCAGCATTAGTAATCAACCTGTTAATCCAAGGTCTTTAGAAAAACT; TGAAATTATTCCTGCAAGCCAATTTTGTCCACGTGTTGAGATCATTGCTACAATGAAAAA; GAAGGGTGAGAAGAGATGTCTGAATCCAGAATCGAAGGCCATCAAGAATTTACTGAAAGC; AGTTAGCAAGGAAAGGTCTAAAAGATCTCCTTAAAACCAGAGGGGAGCAAAATCGATGCA; GTGCTTCCAAGGATGGACCACACAGAGGCTGCCTCTCCCATCACTTCCCTACATGGAGTA; TATGTCAAGCCATAATTGTTCTTAGTTTGCAGTTACACTAAAAGGTGACCAATCATGGTC; ACCAAATCAGCTGCTACTACTCCTGTAGGAAGATTAATGTTCATCATCCTAAGCTATTCA; GTAATAACTCTACCCTGGCACTATAATGTAAGCTCTACTGAGGTGCTATGTTCTTAGTGG; ATGTTCTGACCCTGCTTCAAATATTTCCCTCACCTTTCCCATCTTCCAAGGGTATAAGGA; ATCTTTCTGCTTTGGGGTTTATCAGAATTCTCAGAATCTCAAATAACTAANAGGTATGCA; ATCAAATCTGCTTTTTTAAGAATGCTCTTTACTTCATGGACTTNCACTGCCATCCCTCCA; AGGGGCCCAAATTCTTTCAGTGGCTACCTACATACAATTCCAAACACATACAGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001565 unedited; CCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCTTAGTTATAATTACTTT; ATTAACCTTTTGGTCTTTCAACATTTAGATAGTCTTTCTTAATATTTCCAGGAGAGTACC; TCATTTTTATTTTGAAAACCATTCAGCACATTTATCTTATGTAACATGCAGAGCATATAT; CTATCTGTATTTTTAAAATTTTCCTGTTACTCATTGATACATAGTACTTAATTACATGTT; ATTCCATGTACACTGAAAACAATATAGGAAATATATACATCTAAGACTTCTACTTTGTAC; AGTCTTTCATTAAATAAGAATACTTACACATACATTTTCAGATATTTCTACCTTCCTGTA; TGTGTTTGGAATTGTATGTAGGTAGCCACTGAAAGAATTTGGGCCCCTTGGGAGGATGGC; AGTGGAAGTCCATGAAGTAAAGAGCATTCTTTAAAAAGCAGATTTGATTGCATACCTTTT; AGTTATTTGAGATTCTGAGAATTCTGATAAACCCCAAAGCAGAAAGATTCCTTATACCCT; TGGAAGATGGGAAAGGTGAGGGAAATATTTGAAGCAGGGTCAGAACATNCACTAAGAACA; TAGCCCCCTCAGTAGAAGCTTACATTATANGTGCCAGGGTTAGAGTTATTACTGAATAGC; TTAGGACGATGAACATTAATCTTTCTACAGGAGTAGTAACCGCTGATTTGGGTGACCATG; ATTGGGCCCCCTTTTAATGTACCTGCAAACTAAAACAATTTTGGCTTGACATACCCTCCC; TGTCGGGAAATGATGGGAAAAGGCACCCTTTGTGTGGTCCTTCCCTGGAACCCCTGGCTC; AATTTGGCCCCCCTCCGGTTTAAGGGAAACCTTTACACCTCTCCTGCTAACGGCTCCCAA; AAATCTTGAAGCCCTTCAATTTGGATAAACATCCTTTATCCCCTTTTTGTATGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001565
• Insert Size: 1140 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001565.1, NP_001556.1
• RefSeq Size: 1172 bp
• RefSeq ORF: 297 bp
• Locus ID: 3627
• UniProt ID: P02778
• Cytogenetics: 4q21.1
• Domains: IL8
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway
• Summary: This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. This gene may also be a key regulator of the 'cytokine storm' immune response to SARS-CoV-2 infection. [provided by RefSeq, Sep 2020]