ATP5F1D (NM_001687) Human Untagged Clone

SKU SC119113

ATP5D (untagged)-Human ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit (ATP5D), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ATP5F1D
• Synonyms: ATP5D; MC5DN5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001687 edited; ATGCTGCCCGCCGCGCTGCTCCGCCGCCCGGGACTTGGCCGCCTCGTCCGCCACGCCCGT; GCCTATGCCGAGGCCGCCGCCGCCCCGGCTGCCGCCTCTGGCCCCAACCAGATGTCCTTC; ACCTTCGCCTCTCCCACGCAGGTGTTCTTCAACGGTGCCAACGTCCGGCAGGTGGACGTG; CCCACGCTGACCGGAGCCTTCGGCATCCTGGCGGCCCACGTGCCCACGCTGCAGGTCCTG; CGGCCGGGGCTGGTCGTGGTGCATGCAGAGGACGGCACCACCTCCAAATACTTTGTGAGC; AGCGGTTCCATCGCAGTGAACGCCGACTCTTCGGTGCAGTTGTTGGCCGAAGAGGCCGTG; ACGCTGGACATGTTGGACCTGGGGGCAGCCAAGGCAAACTTGGAGAAGGCCCAGGCGGAG; CTGGTGGGGACAGCTGACGAGGCCACGCGGGCAGAGATCCAGATCCGAATCGAGGCCAAC; GAGGCCCTGGTGAAGGCCCTGGAGTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001687 unedited; GTACAAGTTCCAAACATTTGTTATACGACCTCACTATAGGGCCGGCCGCCGAATTCGGCA; AGAGGGCCTACCCGCTTCCTGCCGCCCGCCGCTTGCCATGCTGCCCGCCGCGCTGCTCCG; CCGCCCGGGACTTGGCCGCCTCGTCCGCCACGCCCGTGCCTATGCCGAGGCCGCCGCCGC; CCCGGCTGCCGCCTCTGGCCCCAACCAGATGTCCTTCACCTTCGCCTCTCCCACGCAGGT; GTTCTTCAACGGTGCCAACGTCCGGCAGGTGGACGTGCCCACGCTGACCGGAGCCTTCGG; CATCCTGGCGGCCCACGTGCCCACGCTGCAGGTCCTGCGGCCGGGGCTGGTCGTGGTGCA; TGCAGAGGACGGCACCACCTCCAAATACTTTGTGAGCAGCGGTTCCATCGCAGTGAACGC; CGACTCTTCGGTGCAGTTGTTGGCCGAAGAGGCCGTGACGCTGGACATGTTGGACCTGGG; GGCAGCCAAGGCAAACTTGGAGAAGGCCCAGGCGGAGCTGGTGGGGACAGCTGACGAGGC; CACGCGGGCAGAGATCCAGATCCGAATCGAGGCCAACGAGGCCCTGGTGAAGGCCCTGGA; GTAGGCGGTGCGTACCCGGTGTCCCGAGGCCCGGCCAGGGGCTGGGCAGGGATGCCAGGT; GGGCCCAGCCAGCTCCTGNGGTCCCGGCCACCTGNGGAAGCCGCGCCTGCCAAGGAGGCC; ACCAGAGGGCAGTGCAGGCTTCTGCCTGGGCCCCAGGCCCTGCCTGTGTTGAAAGCTCTG; GGGACTGGGCCAGGGAAGCTCCTCCTCAGCTTTGAGCTGTGGCTGCCACCCATGGNGCTC; TCCTTCCGCCTCTCAAGATCCCCCCAGCCTGACNGGCCGCTTTACCATCCCTCTGCCCTG; CAGAGCCAGCCGCCAAGGTTGACCTCAGCTTCG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001687 unedited; TTTTTTCAGCTTCCGGGTCTTTATGGGGCGGGGGCTGGGGGCAGTTCATCCATAGTGGCT; CCGAAGCTGAGGTCAACCTTGGCGGCTGGCTCTGCAGGGCATAGGGGATGGTAAGCGGCC; CGTCAGGCTGGGGGGATCTTGAGAGGCGGAAGGAGAGCCCCATGGGTGGCAGCCACAGCT; CAAAGCTGAGGAGGAGCTTCCCTGGCCCATTCCCCAGAGCTTTCAACACAGGCAGGGCCT; GGGGCCCATGCAGAAGCCTGCACTGCCCTCTGGTGGCCTCCTTGGCAGGCGCGGCTTCCC; CATGTGGCCGGGACCCCAGGAGCTGGCTGGGCCCACCTGGCATCCCTGCCCATCCCCTGG; CCGGGCCTCGGGACACCGGGTACGCACCGTCTACTTCAGGGCCTTCACCAGGGCCTCGTT; GGCCTCGATTCGGATCTGGATCTCTGCCCCCGTGGCCTCGTCAGCTGTCCCCACCAGCTC; CGCCTGTGCCTTCNCCAAGCCTCGCCTTGGCTGCCCCCAGGTCCCACATGTCCAGCGTCC; CCGGCCTCCTCGGCCAACAACTGTACCGAATATTCGGCGTTCACTGCGATGGAACCCGTG; CTCCCAAAGTATTTGGAAGTGGGGCCTTTCTCTGCTTGCTCCTAGATCATTCCCTATCCC; AATGACCCGCACCGCGGCTACGCGGCCCCCCCGTTGCCTTTAGGCTTCTGTCTCCCGGCC; CTCTTCCCCTGCAGTCCCTTCGGCCCGCGACTAACCCTTCCTTGCCTACCCATGCCCAAG; CATTTCGTTTTGGCCTACCCTCCTCCTCGCCGGGCTGCCCTTTTCTTGTTCTCTCTGGCG; TCCACTCGTCTATTTTCGCTCGTCTACAACTGCTTCTTTTTTCCCTTCGCCCCGTTCTCA; TCCGCCCCCCGTGGCTAATCTCGTCCCCCCCTTTCCTCACCTTAACTTCTGTCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_001687
• Insert Size: 1020 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001687.4, NP_001678.1
• RefSeq Size: 1005 bp
• RefSeq ORF: 507 bp
• Locus ID: 513
• UniProt ID: P30049
• Cytogenetics: 19p13.3
• Domains: ATP-synt_DE
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Summary: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longer transcript. Both variants encode the same isoform.