Caveolin 1 (CAV1) (NM_001753) Human Untagged Clone

SKU SC119082

CAV1 (untagged)-Human caveolin 1, caveolae protein, 22kDa (CAV1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Caveolin 1
• Synonyms: BSCL3; CGL3; LCCNS; MSTP085; PPH3; VIP21
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001172895 edited; GAATTCGGCACGAGGCTCCTCACAGTTTTCATCCAGCCACGGGCCAGCATGTCTGGGGGC; AAATACGTAGACTCGGAGGGACATCTCTACACCGTTCCCATCCGGGAACAGGGCAACATC; TACAAGCCCAACAACAAGGCCATGGCAGACGAGCTGAGCGAGAAGCAAGTGTACGACGCG; CACACCAAGGAGATCGACCTGGTCAACCGCGACCCTAAACACCTCAACGATGACGTGGTC; AAGATTGACTTTGAAGATGTGATTGCAGAACCAGAAGGGACACACAGTTTTGACGGCATT; TGGAAGGCCAGCTTCACCACCTTCACTGTGACGAAATACTGGTTTTACCGCTTGCTGTCT; GCCCTCTTTGGCATCCCGATGGCACTCATCTGGGGCATTTACTTCGCCATTCTCTCTTTC; CTGCACATCTGGGCAGTTGTACCATGCATTAAGAGCTTCCTGATTGAGATTCAGTGCATC; AGCCGTGTCTATTCCATCTACGTCCACACCGTCTGTGACCCACTCTTTGAAGCTGTTGGG; AAAATATTCAGCAATGTCCGCATCAACTTGCAGAAAGAAATATAAATGACATTTCAAGGA; TAGAAGTATACCTGATTTTTTTTCCTTTTAATTTTCCTGTCAAGTTCCAAGTTGCTAATA; CAGCAACAATTTATGAATTGAATTATCTTGGTTGAAAATAAAAAGATCACTTTCTCAGTT; TTCATAAGTATTATGTCTCTTCTGAGCTATTTCATCTATTTTTGGCAGTCTGAATTTTTA; AAACCCATTTAAATTTTTTTCCTTACCTTTTTATTTGCATGTGGATCAACCATCGCTTTA; TTGGCTGAGATATGAACATATTGTTGAAAGGTAATTTGAGAGAAATATGAAGAACTGAGG; AGGAAAAAAAAAAAAAAGAAAAGAACCAACAACCTCAACTGCCTACTCCAAAATGTTGGT; CATTTTATGTTAAGGGAAGAATTCCAGGGTATGGCCATGGAGTGTACAAGTATGTGGGCA; GATTTTCAGCAAACTCTTTTCCCACTGTTTAAGGAGTTAGTGGATTACTGCCATTCACTT; CATAATCCAGTAGGATCCAGTGATCCTTACAAGTTAGAAAACATAATCTTCTGCCTTCTC; ATGATCCAACTAATGCCTTACTCTTCTTGAAATTTTAACCTATGATATTTTCTGTGCCTG; AATATTTGTTATGTAGATAACAAGACCTCAGTGCCTTCCTGTTTTTCACATTTTCCTTTT; CAAATAGGGTCTAACTCAGCAACTCGCTTTAGGTCAGCAGCCTCCCTGAAGACCAAAATT; AGAATATCCATGACCTAGTTTTCCATGCGTGTTTCTGACTCTGAGCTACAGAGTCTGGTG; AAGCTCACTTCTGGGCTTCATCTGGCAACATCTTTATCCGTAGTGGGTATGGTTGACACT; AGCCCAATGAAATGAATTAAAGTGGACCAATAGGGCTGAGCTCTCTGTGGGCTGGCAGTC; CTGGAAGCCAGCTTTCCCTGCCTCTCATCAACTGAATGAGGTCAGCATGTCTATTCAGCT; TCGTTTATTTTCAAGAATAATCACGCTTTCCTGAATCCAAACTAATCCATCACCGGGGTG; GTTTAGTGGCTCAACATTGTGTTCCCATTTCAGCTGATCAGTGGGCCTCCAAGGAGGGGC; TGTAAAATGGAGGCCATTGTGTGAGCCTATCAGAGTTGCTGCAAACCTGACCCCTGCTCA; GTAAAGCACTTAAAAGATTGAAGTATTTTGCTGGAATAAGTTCAAATTCTTCTGAACTCA; AACTGAGGAATTTCACCTGTAAACCTGAGTCGTACAGAAAGCTGCCTGGTATATCCAAAA; GCTTTTTATTCCTCCTGCTCATATTGTGATTCTGCCTTTGGGGACTTTTCTTAAACCTTC; AGTTATGATTTTTTTTTCATACACTTATTGGAACTCTGCTTGATTTTTGCCTCTTCCAGT; CTTCCTGACACTTTAATTACCAACCTGTTACCTACTTTGACTTTTTGCATTTAAAACAGA; CACTGGCATGGATATAGTTTTACTTTTAAACTGTGTACATAACTGAAAATGTGCTATACT; GCATACTTTTTAAATGTAAAGATATTTTTATCTTTATATGAAGAAAATCACTTAGGAAAT; GGCTTTGTGATTCAATCTGTAAACTGTGTATTCCAAGACATGTCTGTTCTACATAGATGC; TTAGTCCCTCATGCAAATCAATTACTGGTCCAAAAGATTGCTGAAATTTTATATGCTTAC; TGATATATTTTACAATTTTTTATCATGCATGTCCTGTAAAGGTTACAAGCCTGCACAATA; AAAATGTTTAACGGTTAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001753 unedited; TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCTCACAGTTTTC; ATCCAGCCACGGGCCAGCATGTCTGGGGGCAAATACGTAGACTCGGAGGGACATCTCTAC; ACCGTTCCCATCCGGGAACAGGGCAACATCTACAAGCCCAACAACAAGGCCATGGCAGAC; GAGCTGAGCGAGAAGCAAGTGTACGACGCGCACACCAAGGAGATCGACCTGGTCAACCGC; GACCCTAAACACCTCAACGATGACGTGGTCAAGATTGACTTTGAAGATGTGATTGCAGAA; CCAGAAGGGACACACAGTTTTGACGGCATTTGGAAGGCCAGCTTCACCACCTTCACTGTG; ACGAAATACTGGTTTTACCGCTTGCTGTCTGCCCTCTTTGGCATCCCGATGGCACTCATC; TGGGGCATTTACTTCGCCATTCTCTCTTTCCTGCACATCTGGGCAGTTGTACCATGCATT; AAGAGCTTCCTGATTGAGATTCAGTGCATCAGCCGTGTCTATTCCATCTACGTCCACACC; GTTTGTGACCCACTCTTTGAAGCTGTTGGGAAAATATTCAGCAATGTCCGCATCACCTTG; CAGAAAGAAATATAAATGACATTTTCAGGATAGAAGTATACCTGATTTTTTTTTCCTTTT; AATCTTCCTGTCAAGTTCCAAGTTGCTAATACAGCAACAATTTATGAATTGAATTACCTC; GGTTGAAAAAAAAAGATCACTTTCTCAGTTTTCATAAGAATTATGCTCTTCTGAGCTATT; TCATCTATTTTGGGCAGCCTGAATTTTAAAACCCCATTAAATTTTTTCCCTTCCCTTTTT; ATTTGAAGGGGGAACAACCATCCCTTTATTGGCTGGAGAAATCAACTACTGTGTGGAGAG; TTATTTGGGCAAAATTTTGACAAACTGGGGAGGAAAAAAAAACAAGAAATGCAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001753 unedited; ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACCGTTAAACATTTT; TATTGTGCAGGCTTGTAACCTTTACAGGACATGCATGATAAAAAATTGTAAAATATATCA; GTAAGCATATAAAATTTCAGCAATCTTTTGGACCAGTAATTGATTTGCATGAGGGACTAA; GCATCTATGTAGAACAGACATGTCTTGGAATACACAGTTTACAGATTGAATCACAAAGCC; ATTTCCTAAGTGATTTTCTTCATATAAAGATAAAAATATCTTTACATTTAAAAAGTATGC; AGTATAGCACATTTTCAGTTATGTACACAGTTTAAAAGTAAAACTATATCCATGCCAGTG; TCTGTTTTAAATGCAAAAAGTCAAAGTAGGTAACAGGTTGGTAATTAAAGTGTCAGGAAG; ACTGGAAGAGGCAAAAATCAAGCAGAGTTCCAATAAGTGTATGAAAAAAAAATCATAACT; GAAGGTTTAAGAAAAGTCCCCAAAGGCAGAATCACAATATGAGCAGGAGGAATAAAAAGC; TTTTGGATATACCAGGCAGCTTTCTGTACGACTCAGGTTTACAGGTGAAATTCCTCAGTT; TGAGTTCAGAAGAATTTGAACTTATTCCAGCAAAATACTTCAATCTTTTAAGTGCTNTAC; TGAGCANGGGTCANGGTTGCAGCAACTCTGATAGGCTCACACAATGGCCTNCATTTTACA; GCCCCTCCTTGGNAGGCCACTGATCAGCTGAAATGGGAAACACATGNTGAGCCACTAAAC; CACCCCGGTGATGGGATAGTTTGGATCAGGAAAGCGTGATTATTCTGAAAATAACGAGCT; GAATAGACTGCTGACCTCATCANTGATGAGAGCAGGNAAAGCTGCTTNCAGATGNCAGCC; ACAGAGAGCTAGCCTATGGNNCCTTNNATCATTCATGGGCTANGTCACCTCACACTACGA; TAAGATGTGCGATGAGCCAAAGTGGACTACCAACTTTGTGCTAAGTCGAAACAGCTGAAA; CTAGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_001753
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: A TrueClone.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001753.3, NP_001744.2
• RefSeq Size: 2704 bp
• RefSeq ORF: 537 bp
• Locus ID: 857
• UniProt ID: Q03135
• Cytogenetics: 7q31.2
• Domains: Caveolin
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Focal adhesion, Viral myocarditis
• Summary: The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]; Transcript Variant: This variant (1) encodes the longer isoform (alpha).