Cyclin D2 (CCND2) (NM_001759) Human Untagged Clone

SKU SC119036

CCND2 (untagged)-Human cyclin D2 (CCND2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Cyclin D2
• Synonyms: KIAK0002; MPPH3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119036 sequence for NM_001759 edited (data generated by NextGen Sequencing); ATGGAGCTGCTGTGCCACGAGGTGGACCCGGTCCGCAGGGCCGTGCGGGACCGCAACCTG; CTCCGAGACGACCGCGTCCTGCAGAACCTGCTCACCATCGAGGAGCGCTACCTTCCGCAG; TGCTCCTACTTCAAGTGCGTGCAGAAGGACATCCAACCCTACATGCGCAGAATGGTGGCC; ACCTGGATGCTGGAGGTCTGTGAGGAACAGAAGTGCGAAGAAGAGGTCTTCCCTCTGGCC; ATGAATTACCTGGACCGTTTCTTGGCTGGGGTCCCGACTCCGAAGTCCCATCTGCAACTC; CTGGGTGCTGTCTGCATGTTCCTGGCCTCCAAACTCAAAGAGACCAGCCCGCTGACCGCG; GAGAAGCTGTGCATTTACACCGACAACTCCATCAAGCCTCAGGAGCTGCTGGAGTGGGAA; CTGGTGGTGCTGGGGAAGTTGAAGTGGAACCTGGCAGCTGTCACTCCTCATGACTTCATT; GAGCACATCTTGCGCAAGCTGCCCCAGCAGCGGGAGAAGCTGTCTCTGATCCGCAAGCAT; GCTCAGACCTTCATTGCTCTGTGTGCCACGGACTTTAAGTTTGCCATGTACCCACCGTCG; ATGATCGCAACTGGAAGTGTGGGAGCAGCCATCTGTGGGCTCCAGCAGGATGAGGAAGTG; AGCTCGCTCACTTGTGATGCCCTGACTGAGCTGCTGGCTAAGATCACCAACACAGACGTG; GATTGTCTCAAAGCTTGCCAGGAGCAGATTGAGGCGGTGCTCCTCAATAGCCTGCAGCAG; TACCGTCAGGACCAACGTGACGGATCCAAGTCGGAGGATGAACTGGACCAAGCCAGCACC; CCTACAGACGTGCGGGATATCGACCTGTGA; ; Clone variation with respect to NM_001759.3; 570 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001759 unedited; TACGACTCTCTATAGGGCGGCCGCGAATTCGGCACGAGGGTGCGAGTGAGGCAGCCCCGA; GGCTCTGCTCGCCCACCACCCAATCCTCGCCTCCCTTCTGCTCCACCTTCTCTCTCTGCC; CTCACCTCTCCCCCGAAAACCCCCTATTTAGCCAAAGGAAGGAGGTCAGGGGAACGCTCT; CCCCTCCCCTTCCAAAAAACAAAAACAGAAAAACCCTTTTCCAGGCCGGGGAAAGCAGGA; GGGAGAGGGGCCGCCGGGCTGGCCATGGAGCTGCTGTGCCACGAGGTGGACCCGGTCCGC; AGGGCCGTGCGGGACCGCAACCTGCTCCGAGACGACCGCGTCCTGCAGAACCTGCTCACC; ATCGAGGAGCGCTACCTTCCGCAGTGCTCCTACTTCAAGTGCGTGCAGAAGGACATCCAA; CCCTACATGCGCAGAATGGTGGCCACCTGGATGCTGGAGGTCTGTGAGGAACAGAAGTGC; GAAGAAGAGGTCTTCCCTCTGGCCATGAATTACCTGGACCGTTTCTTGGCTGGNGTCCCG; ACTCCGAAGTCCCATCTGCAACTCCTGGGTGCTGTCTGCATGTTCCTGGCCTCCAAACTC; AAGAGACCAGCCCGCTGNACCGCGAGAAGCTGTGCATTTACACCCGACACTNCATTCAGC; CTCAAGAGCTGCTGGAGTGGAAACTGGTGTGCTGGGGAAGTTGAAGTGGAAACCTGCACC; TGTCACTCCTCATGAACTCATTGAGCACATTTTTGCCCAAGCTGGCCCCACACCGGGAGA; AACTGTTTCTGGATCGCAAGCATGCTTCAAACCTCATTGGTCTTGGGGGCCACGGCCTTT; AGTTTGCATGGCCCCCCCGCATGATCCCACTGTAATGGGGGAACACCCTTGGGGGCCTCA; CCAGAGAGGAAGGACCCCCTCATGGGAGCCCTCACTGACGCTGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001759 unedited; GCCGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTGCTGTACTTCAAATAGGCACCAA; ATGCTTTTTATTATTCCGTTTTCGTAGGACCTTTTTCTCTCACTTCTAAAAGATCTTTAA; ATATGATCTAAGTGGGGGCAGAATTTTTTTTTTAAGTTTCACCCTAAAACACAAAGAACA; AAAACAACCAGAAAGAAGAAGAGACCAGATTATGGACGCGTCTCTCTCTTTCGGCCCAAC; TGGCATCCTCACAGGTCGATATCCCGCACGTCTGTAAGGGTGCTGGCTTGGTCCAGTTCA; TCCTCCGACTTGGATCCGTCACGTTGGTCCTGACGGTACTGCTGCAGGCTATTGAGGAGC; ACCGCCTCAATCTGCTCCTGGCAAGCTTTGAGACAATCCACGTCTGTGTTGGTGATCTTA; GCCAGCAGCTCAGTCAGGGCATCACAAGTGAGCGAGCTCACTTCCTCATCCTGCTGGAGC; CCACAGATGGCTGCTCCCACACTTCCAGTTGCGATCATCGACGGTGGGTACATGGCAAAC; TTAAAGTCCGTGGCACACAGAGCAATGAAAGTCTGAGCATGCTTGCGGATCAGAGACAGC; TTCTCCCGCTGCTGGGGCAGCTTGCGCAAGATGTGCTCAATGAAGTCATGAGGAGTGACA; GCTGCCAGGTTCCACTTAAATTACCCAGCACAACAGTTCCACTTCAGCAGCTCCTGAAGC; TTGAGGAGTTGTCCGGGTATATGCCAAGTTCTCCGCGGGCAACGGGGCTGGTTTTCTTTG; ATTTTGAAGGCCCAGAACATGCAAACACGACCCAGAAATTTGCAATGGTATTTTGGAATT; CGGGACCCCATCCATAAAACGGTCACGCTATTCATGGGCCAGAGGGAAAACCCTCTTTTT; GCACTCTTGGTCCTTACATAATCTCAATCCCCAGGTGGCACCATTCTGCCCAGGAGCGGG; GGATTGCCTTTCCGCCCCCTTGAAGAAGAGCCCTGGCAAAAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001759
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001759.2, NP_001750.1
• RefSeq Size: 6480 bp
• RefSeq ORF: 870 bp
• Locus ID: 894
• UniProt ID: P30279
• Cytogenetics: 12p13.32
• Domains: cyclin, CYCLIN, cyclin_C
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Focal adhesion, Jak-STAT signaling pathway, p53 signaling pathway, Wnt signaling pathway
• Summary: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3). [provided by RefSeq, Sep 2014]