Cadherin 8 (CDH8) (NM_001796) Human Untagged Clone

SKU SC119022

CDH8 (untagged)-Human cadherin 8, type 2 (CDH8)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Cadherin 8
• Synonyms: Nbla04261
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119022 sequence for NM_001796 edited (data generated by NextGen Sequencing); ATGCCAGAACGGCTAGCGGAAATGCTCTTGGATCTCTGGACTCCATTAATAATATTATGG; ATTACTCTTCCCCCTTGCATTTACATGGCTCCGATGAATCAGTCTCAAGTTTTAATGAGT; GGATCCCCTTTGGAACTAAACAGTCTGGGTGAAGAACAGCGAATTTTGAACCGCTCCAAA; AGAGGCTGGGTTTGGAATCAAATGTTTGTCCTGGAAGAGTTTTCTGGACCTGAACCGATT; CTTGTTGGCCGGCTACACACAGACCTGGATCCTGGGAGCAAAAAAATCAAGTATATCCTA; TCAGGTGATGGAGCTGGGACCATATTTCAAATAAATGATGTAACTGGAGATATCCATGCT; ATAAAAAGACTTGACCGGGAGGAAAAGGCTGAGTATACCCTAACAGCTCAAGCAGTGGAC; TGGGAGACAAGCAAACCTCTGGAGCCTCCTTCTGAATTTATTATTAAAGTTCAAGACATC; AATGACAATGCACCAGAGTTTCTTAATGGACCCTATCATGCTACTGTGCCAGAAATGTCC; ATTTTGGGTACATCTGTCACTAACGTCACTGCGACCGACGCTGATGACCCAGTTTATGGA; AACAGTGCAAAGTTGGTTTATAGTATATTGGAAGGGCAGCCTTATTTTTCCATTGAGCCT; GAAACAGCTATTATAAAAACTGCCCTTCCCAACATGGACAGAGAAGCCAAGGAGGAGTAC; CTGGTTGTTATCCAAGCCAAAGATATGGGTGGACACTCTGGTGGCCTGTCTGGGACCACG; ACACTTACAGTGACTCTTACTGATGTTAATGACAATCCTCCAAAATTTGCACAGAGCCTG; TATCACTTCTCAGTACCGGAAGATGTGGTTCTTGGCACTGCAATAGGAAGGGTGAAGGCC; AATGATCAGGATATTGGTGAAAATGCACAGTCATCATATGATATCATCGATGGAGATGGA; ACAGCACTTTTTGAAATCACTTCTGATGCCCAGGCCCAGGATGGCATTATAAGGCTAAGA; AAACCTCTGGACTTTGAGACCAAAAAATCCTATACGCTAAAGGTAGAGGCAGCCAATGTC; CATATTGACCCACGCTTCAGTGGCAGGGGGCCCTTTAAAGACACGGCGACAGTCAAAATC; GTGGTTGAAGATGCTGATGAGCCTCCGGTCTTCTCTTCACCGACTTACCTACTTGAAGTT; CATGAAAATGCTGCTCTAAACTCCGTGATTGGGCAAGTGACTGCTCGTGACCCTGATATC; ACTTCCAGTCCTATAAGGTTTTCCATCGACCGGCACACTGACCTGGAGAGGCAGTTCAAC; ATTAATGCAGACGATGGGAAGATAACGCTGGCAACACCACTTGACAGAGAATTAAGTGTA; TGGCACAACATAACAATCATTGCTACTGAAATTAGGAACCACAGTCAGATATCACGAGTA; CCTGTTGCTATTAAAGTGCTGGATGTCAATGACAACGCCCCTGAATTCGCATCCGAATAT; GAGGCATTTTTATGTGAAAATGGAAAACCCGGCCAAGTCATTCAAACTGTTAGCGCCATG; GACAAAGATGATCCCAAAAACGGACATTATTTCTTATACAGTCTCCTTCCAGAAATGGTC; AACAATCCGAATTTCACCATCAAGAAAAATGAAGATAATTCCCTCAGTATTTTGGCAAAG; CATAATGGATTCAACCGCCAGAAGCAAGAAGTCTATCTTTTACCAATCATAATCAGTGAT; AGTGGAAATCCTCCACTGAGCAGCACTAGCACCTTGACAATCAGGGTCTGTGGCTGCAGC; AATGACGGTGTCGTCCAGTCTTGCAATGTCGAAGCTTATGTCCTTCCAATTGGACTCAGT; ATGGGCGCCTTAATTGCCATATTAGCATGCATCATTTTGCTGTTAGTCATCGTGGTGCTG; TTTGTAACTCTACGGCGGCATAAAAATGAACCATTAATTATCAAAGATGATGAAGACGTT; CGAGAAAACATCATTCGCTACGATGATGAAGGAGGAGGGGAGGAGGACACAGAGGCTTTT; GACATTGCAACTTTACAAAATCCAGATGGAATTAATGGATTTTTACCCCGTAAGGATATT; AAACCAGATTTGCAGTTTATGCCAAGGCAAGGGCTTGCTCCAGTTCCAAATGGTGTTGAT; GTCGATGAATTTATAAATGTAAGGCTGCATGAGGCAGATAATGATCCCACGGCCCCGCCA; TATGACTCCATTCAGATATATGGCTATGAAGGCCGAGGGTCAGTGGCTGGCTCCCTCAGC; TCCTTGGAGTCCACCACATCAGACTCAGACCAGAATTTTGACTACCTCAGTGACTGGGGT; CCCCGCTTTAAGAGACTGGGCGAACTCTACTCTGTTGGTGAAAGTGACAAAGAAACTTGA; ; Clone variation with respect to NM_001796.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001796 unedited; AAGCTTTGGATTTGTAATCCNACTTACTATAGNNGGCGGCCGCGCAATTCGCACGAGGGC; CGCCGCCGCCGGCTTGTGCATAGACGGTAGCGTCAAGACACCAGCTGCACTTTGGGTTGC; GGACACCCAGAGCCCAGCGCGCCCGCCCTGCGTCTCCGCACCAAGCCCTGTAGCTCCGAG; AGGCATGAACGGAATCCGGAGGCGCCTGCCTAGCGAGCGAGGACCGGTCGGCGCTTGCCG; CCCTCGGGAGCTAACCCCGAGCACCTCCAGCCATTTGTGAACCTGGAGGCTTGACATTCG; CCAGCGCAGGGCCCCACAAGAGAAATTTCAATGAAAAGAAAAGCCAATGGATTGTGGTCT; TAGAAAAGCTGCTTAGATGATGTCTGTTTCCCGTGCTATAGACACGTGGCAGAGCTGTAA; GTAAATGCTCGGCACTGCATGATGAATTGGATGGCTGCAGACCGGAGACAAAAAAAATAA; TTGTCTCATTTTCGTGGTGATTTGCTTAACTGGTGGGACCATGCCAGAACGGCTAGCGGA; AATGCTCTTGGATCTCTGGACTCCATTAATAATATTATGGATTACTCTTCCCCCTTGCAT; TTACATGGCTCCGATGAATCAGTCTCAAGTTTTAATGAGTGGATCCNCTTTGGAACTAAA; CAGTCTGGGTGAAGAACAGCGAATTTTGAACCGCTCCAAAAGAGGCTGGGTTTGGAATCA; AATGTTTGTCCTGGAAGAGTTTCTGGACCTGAACCGATTCTTGTTGGCCGGCTACACACA; GACCTGGATCCTGNGAGCANAAAAATCAAGTATATCCTATCAGGTGATGGAGCTGGGACC; ATATTTCAATNNAATGATGTACTGGAGATATCCATGCTATAAAAAGACTTGACCGGGGAG; AAAAAGCTGAGTATACCCNNTACAGCTCAGCAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001796 unedited; GCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTCAACATTAAAATG; TGGTTGAGTTTATAGATGACTGGTGCTAAACTTGCCTCTAAAACAAATAGCCACATTGGT; TGTATCTAAGGGGAGTGACCCTAGAATATTACAGAATGCTCAGTTCCAGTGATTTATTTA; TAATCCACTGTCAAGTTTCTTTGTCACTTTCACCAACAGAGTAGAGTTCGCCCAGTCTCT; TAAAGCGGGGACCCCAGTCACTGAGGTAGTCAAAATTCTGGTCTGAGTCTGATGTGGTGG; ACTCCAAGGAGCTGAGGGAGCCAGCCACTGACCCTCGGCCTTCATAGCCATATATCTGAA; TGGAGTCATATGGCGGGGCCGTGGGATCATTATCTGCCTCATGCAGCCTTACATTTATAA; ATTCATCGACATCAACACCATTTGGAACTGGAGCAAGCCCTTGCCTTGGCATAAACTGCA; AATCTGGTTTAATATCCTTACGGGGTAAAAATCCATTAATTCCATCTGGATTTTGTAAAG; TTGCAATGTCAAAAGCCTCTGTGTCCTCCTCCCCTCCTCCTTCATCATCGTAGCGAATGA; TGTTTTCTCGAACGTCTTCATCATCTTTGATAATTAATGGTTCATTTTTATGCCGCCGTA; GAGTTACAAACAGCACCACGATGACTAACAGCAAAATGATGCATGCTAATATGGCAATTA; AGGCGCCCATACTGAGTNCAATTGAAGGACATNNAGCTCGACATTGCAGACTGGACGACA; CCGTCATTGCTGCAGCCACAGACCCTGATGTCAAGGGGCTAGTGCTGCTCATGGNAGATT; TTCCCTTCACTGATATGGATGGTAAAGATAACTTCTGCTNCTGGCGNTGAATCATTATGC; TTTGCCAATACTGAGGAATATCTTCATTTCCTTGTGTGGATTCCGATGTTGACATTCTGG; ANGAGATGNTANAAAAAAGGCCGTTTGGGAACACCTTGCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_001796
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001796.2, NP_001787.2
• RefSeq Size: 2929 bp
• RefSeq ORF: 2400 bp
• Locus ID: 1006
• UniProt ID: P55286
• Cytogenetics: 16q21
• Domains: CA, Cadherin_C_term
• Protein Families: Transmembrane
• Summary: This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed in brain and is putatively involved in synaptic adhesion, axon outgrowth and guidance. [provided by RefSeq, Jul 2008]