CRHBP (NM_001882) Human Untagged Clone

SKU SC118962

CRHBP (untagged)-Human corticotropin releasing hormone binding protein (CRHBP)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CRHBP
• Synonyms: CRF-BP; CRFBP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118962 sequence for NM_001882 edited (data generated by NextGen Sequencing); ATGTCGCCCAACTTCAAACTTCAGTGTCACTTCATTCTCATCTTCCTGACGGCTCTAAGA; GGGGAAAGCCGGTACCTAGAGCTGAGGGAAGCGGCGGACTACGATCCTTTCCTGCTCTTC; AGCGCCAACCTGAAGCGGGAGCTGGCTGGGGAGCAGCCGTACCGCCGCGCTCTGCGGTGC; CTGGACATGCTGAGCCTCCAGGGCCAGTTCACCTTCACCGCCGACCGGCCGCAGCTGCAC; TGCGCAGCCTTCTTCATCAGCGAGCCCGAGGAGTTCATTACCATCCACTACGACCAGGTC; TCCATCGACTGTCAGGGCGGCGACTTCCTGAAGGTATTTGATGGTTGGATTCTCAAGGGG; GAGAAGTTCCCCAGTTCCCAGGATCATCCTCTCCCCTCAGCTGAGCGGTACATAGATTTC; TGTGAGAGTGGTCTTAGCAGGAGGAGCATCAGATCTTCCCAGAATGTGGCCATGATCTTC; TTCCGAGTCCATGAACCAGGAAATGGATTCACATTAACCATAAAGACAGACCCCAACCTC; TTTCCTTGCAATGTCATTTCTCAGACTCCAAATGGAAAGTTTACCCTGGTAGTTCCACAC; CAGCATCGAAACTGCAGCTTCTCCATAATTTATCCTGTGGTGATCAAAATATCTGATCTT; ACCCTGGGACACGTAAATGGTCTTCAGTTAAAGAAATCCTCAGCAGGTTGCGAGGGAATA; GGAGACTTTGTGGAGCTGCTGGGAGGAACTGGATTGGACCCTTCCAAGATGACGCCTTTA; GCTGATCTCTGCTACCCCTTTCATGGCCCGGCCCAGATGAAAGTTGGCTGTGACAACACT; GTGGTGCGCATGGTCTCCAGTGGAAAACACGTAAATCGTGTGACTTTTGAGTATCGTCAG; CTGGAGCCGTACGAGCTGGAAAACCCAAATGGAAACAGTATCGGGGAATTCTGTTTGTCT; GGTCTTTGA; ; Clone variation with respect to NM_001882.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001882 unedited; ACCCGATTTGTAACCGNACTTCATAGTAGGCGGCCAGCACATTCGGCACGAGGGTCTGCG; CGATGGTGTAGGAAGGAAAGCCCAGGACCTCCGGAGCAGAGCACAGCAGCTGCAGAGGCA; AGGCCAGCATGTCGCCCAACTTCAAACTTCAGTGTCACTTCATTCTCATCTTCCTGACGG; CTCTAAGAGGGGAAAGCCGGTACCTAGAGCTGATTTTTCGGCGGACTACGATCCTTTCCT; GCTCTTCAGCGCCAACCTGAAGCGGGAGCTGGCTGGGGAGCAGCCGTACCGCCGCGCTCT; GCGGTGCCTGGACATGCTGAGCCTCCAGGGCCAGTTCACCTTCACCGCCGACCGGCCGCA; GCTGCACTGCGCAGCCTTCTTCATCAGCGAGCCCGAGGAGTTCATTACCATCCACTACGA; CCAGGTCTCCATCGACTGTCAGGGCGGCGACTTCCTGAAGGTATTTGATGGTTGGATTCT; CAAGGGGGAGAAGTTCCCCAGTTCCCAGGATCATCCTCTCCCCTCAGCTGAGCGGTACAT; AGATTTCTGTGAGAGTGGTCTTAGCAGGAGGAGCATCAGATCTTCCCAGAATGTGGCCAT; GATCTTCTTCCGAGTCCATGAACCAGGAAATGGATTCACATTAACCATAAAGACAGACCC; CAACCTCTTTTCTTGNCATGTCATTTCTCAGACTCCAATGGAAAGTTTACCCTGGTAGTT; CCACACCAGCATCGAAACTGCAGCTTCTCCATAATTTATCCTGTGGTGATCAAAATATCT; GATCTTACCCTGGGACACGTAAATGGGTCTTCAGTTAAAAAAATCCCTCANCAAGGTGCG; AGGGAATTAGAGAACTTTGTGAGCTGCTGGNNAGAACTGGATTGAACCTTCCAGAAGACG; CCTTTGCTGATCTCTGCTACCCCTTCAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001882 unedited; GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCCACATTCTGAATGTATTTATTCAT; GTTTGTATTTGTGGATGGAAAATTTTTTCCATGCTACAAAGTTTGGTTAAAAACAAGTTG; TTACAAATCAATAAATAATTGCTATAACTATTACCTATTGTAACAATAATAAATAGAAAA; TAAACAGATGCAACTACTTTGCTATACACAAGACTTTATCCTGTTATAAAACTAATATAA; GTGCTGCCACTTTTCTTTCTTTGAGTATTTTCCCTTGAGATACGATTTTTGTATTGCACG; TTGCTTTGTGCTTGTCTTACCAGTTTCAGGTTTGTAGCACATCAAAGAATTACAAACATT; TCCATTTAGAACTATGATCCTACCAATCAGGGGTTATTTTCTGCCATGTGTTCATTTACA; GATTACAAACATAAAAGAAGCAAAGTACAAAAATTAATGCGTGTGTGTATGTGTGTGTGT; GTGTGCGCGCGTGCGCTCAAGGCTGGGAATACTGACTGGTATGAAAGGCTTTTAACTAGT; TGTGCATCAAAATCATACACAATGGCCATTAAAAACTCACTTAGCTATCAGCATGTAAAT; CACTGGGTTGGTTATTCAAAGACCAGACAAACAGAATTCCCCGATACTGTTTCCATTTGG; GTTTTCCAGCTCGTACGGCTTCAGCTGACGATACTCAAAAGTCACACGATTTACGTGTTT; TCCACTACANACATGCGCACCACAGTGTTGTCACAGCCAAACTTTCATCTGGCCCGGGCC; ATGAAAAGGGGTANCAAAGATCAGCTAAAGGCGTCATCTTGGAAGGGNTCCATTCAGNTC; CTTCCAGCAGCTCACCAAAGTTTCCTATCCCTCGCAACCTGCTGAGGATTCTTTACTGAG; ACCATTTACGGTCCACGGTAGATCAAAATTTGATCACACAGAAAATTTGGGAAGCTGCGT; TCTAGCTGTGTGGACTACAAGGAACACTCACTTGAGTTAGAAGACATGCAGTAAAGTGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001882
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001882.3, NP_001873.2
• RefSeq Size: 1854 bp
• RefSeq ORF: 969 bp
• Locus ID: 1393
• UniProt ID: P24387
• Cytogenetics: 5q13.3
• Summary: Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH-binding protein which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy. [provided by RefSeq, Jul 2008]