Endothelin A Receptor (EDNRA) (NM_001957) Human Untagged Clone

SKU SC118901

EDNRA (untagged)-Human endothelin receptor type A (EDNRA), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Endothelin A Receptor
• Synonyms: ET-A; ETA; ETA-R; ETAR; ETRA; hET-AR; MFDA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC118901 representing NM_001957.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCGGCCGCGAATTCGGCACGAGGGTCTGACGATTGTGGAGAGGCGGTGGAGAGGCTTCATCCATCCCAC; CCGGTCGTCGCCGGGGATTGGGGTCCCAGCGAGACCTCCCCGGGAGAAGCAGTGCCCAGGAGGTTTTCT; GAAGCCGGGGAAGCTGTGCAGCCGAAGCCGCCGCCGCGCCGGAGCCCGGGACACCGGCCACCCTCCGCG; CCACCCACCCTCGCCGGCTCCGGCTTCCTCTGGCCCAGGCGCCGCGCGGACCCGGCAGCTGTCTGCGCA; CGCCGAGCTCCACGGTGAAAAAAAAGTGAAGGTGTAAAAGCAGCACAAGTGCAATAAGAGATATTTCCT; CAAATTTGCCTCAAGATGGAAACCCTTTGCCTCAGGGCATCCTTTTGGCTGGCACTGGTTGGATGTGTA; ATCAGTGATAATCCTGAGAGATACAGCACAAATCTAAGCAATCATGTGGATGATTTCACCACTTTTCGT; GGCACAGAGCTCAGCTTCCTGGTTACCACTCATCAACCCACTAATTTGGTCCTACCCAGCAATGGCTCA; ATGCACAACTATTGCCCACAGCAGACTAAAATTACTTCAGCTTTCAAATACATTAACACTGTGATATCT; TGTACTATTTTCATCGTGGGAATGGTGGGGAATGCAACTCTGCTCAGGATCATTTACCAGAACAAATGT; ATGAGGAATGGCCCCAACGCGCTGATAGCCAGTCTTGCCCTTGGAGACCTTATCTATGTGGTCATTGAT; CTCCCTATCAATGTATTTAAGCTGCTGGCTGGGCGCTGGCCTTTTGATCACAATGACTTTGGCGTATTT; CTTTGCAAGCTGTTCCCCTTTTTGCAGAAGTCCTCGGTGGGGATCACCGTCCTCAACCTCTGCGCTCTT; AGTGTTGACAGGTACAGAGCAGTTGCCTCCTGGAGTCGTGTTCAGGGAATTGGGATTCCTTTGGTAACT; GCCATTGAAATTGTCTCCATCTGGATCCTGTCCTTTATCCTGGCCATTCCTGAAGCGATTGGCTTCGTC; ATGGTACCCTTTGAATATAGGGGTGAACAGCATAAAACCTGTATGCTCAATGCCACATCAAAATTCATG; GAGTTCTACCAAGATGTAAAGGACTGGTGGCTCTTCGGGTTCTATTTCTGTATGCCCTTGGTGTGCACT; GCGATCTTCTACACCCTCATGACTTGTGAGATGTTGAACAGAAGGAATGGCAGCTTGAGAATTGCCCTC; AGTGAACATCTTAAGCAGCGTCGAGAAGTGGCAAAAACAGTTTTCTGCTTGGTTGTAATTTTTGCTCTT; TGCTGGTTCCCTCTTCATTTAAGCCGTATATTGAAGAAAACTGTGTATAACGAGATGGACAAGAACCGA; TGTGAATTACTTAGTTTCTTACTGCTCATGGATTACATCGGTATTAACTTGGCAACCATGAATTCATGT; ATAAACCCCATAGCTCTGTATTTTGTGAGCAAGAAATTTAAAAATTGTTTCCAGTCATGCCTCTGCTGC; TGCTGTTACCAGTCCAAAAGTCTGATGACCTCGGTCCCCATGAACGGAACAAGCATCCAGTGGAAGAAC; CACGATCAAAACAACCACAACACAGACCGGAGCAGCCATAAGGACAGCATGAACTGACCACCCTTAGAA; GCACTCCTCGGTACTCCCATAATCCTCTCGGAGAAAAAAATCACAAGGCAACTGTGAGTCCGGGAATCT; CTTCTCTGATCCTTCTTCCTTAATTCACTCCCACACCCAAGAAGAAATGCTTTCCAAAACCGCAAGGGT; AGACTGGTTTATCCACCCACAACATCTACGAATCGTACTTCTTTAATTGATCTAATTTACATATTCTGC; GTGTTGTATTCAGCACTAAAAAATGGTGGGAGCTGGGGGAGAATGAAGACTGTTAAATGAAACCAGAAG; GATATTTACTACTTTTGCATGAAAATAGAGCTTTCAAGTACATGGCTAGCTTTTATGGCAGTTCTGGTG; AATGTTCAATGGGAACTGGTCACCATGAAACTTTAGAGATTAACGACAAGATTTTCTACTTTTTTTAAG; TGATTTTTTTGTCCTTCAGCCAAACACAATATGGGCTCAAGTCACTTTTATTTGAAATGTCATTTGGTG; CCAGTATTTTTTAACTGCATAATAGCCTAACATGATTATTTGAACTTATTTACACATAGTTTGAAAAAA; AAAAGACAAAAATAGTATTCAGGTGAGCAATTAGATTAGTATTTTCCACGTCACTGTTTATTTTTTTAA; AACACAAATTCTAAAGCTACAACAAATACTACAGGCCCTTAAAGCACAGTCTGATGACACATTTGGCAG; TTTAATAGATGTTACTCAAAGAATTTTTTAAGAACTGTATTTTATTTTTTAAATGGTGTTTTATTACAA; GGGACCTTGAACATGTTTTGTATGTTAAATTCAAAAGTAATGCTTCAATCAGATAGTTCTTTTTCACAA; GTTCAATCTGTTTTTCATGTAAATTTTGTATGAAAAATCAATGTCAAGTACCAAAATGTTAATGTATGT; GTCATTTAACTCTGCCTGAGACTTTCAGTGCACTGTATATAGAAGTCTAAAACACACCTAAGAGAAAAA; GATCGAATTTTTCAGATGATTCAGAAATTTTCATTCAGGTATTTGTAATAGTGACATATATATGTATAT; ACATATCACCTCCTATTCTCTTAATTTTTCTTAAAATGTTAACTGGCAGTAAGTCTTTTTTGATCATTC; CCTTTTCCATATAGGAAACATAATTTTGAAGTGGCCAGATGAGTTTATCATGTCAGTGAAAAATAATTA; CCCACAAATGCCACCAGAACTTACGATTCTTCACTTCTTGGGGTTTTCAGTATGAACCTAACTCCCCAC; CCCAACATCTCCCTCCCACATTGTCACCATTTCAAAGGGCCCACAGTGACTTTTGCTGGGCATTTTCCC; AGATGTTTACAGACTGTGAGTACAGCAGAAAATCTTTTACTAGTGTGTGTGTGTATATATATAAACAAT; TGTAAATTTCTTTTAGCCCATTTTTCTAGACTGTCTCTGTGGAATATATTTGTGTGTGTGATATATGCA; TGTGTGTGATGGTATGTATGGATTTAATCTAATCTAATAATTGTGCCCCGCAGTTGTGCCAAAGTGCAT; AGTCTGAGTAAAATCTAGGTGATTGTTCATCATGACAACCTGCCTCAGTCCATTTTAACCTGTAGCAAC; CTTCTGCATTCATAAATCTTGTAATCATGTTACCATTACAAATGGGATATAAGAGGCAGCGTGAAAGCA; GATGAGCTGTGGACTAGCAATATAGGGTTTTGTTTGGTTGGTTGGTTTGATAAAGCAGTATTTGGGGTC; ATATTGTTTCCTGTGCTGGAGCAAAAGTCATTACACTTTGAAGTATTATATTGTTCTTATCCTCAATTC; AATGTGGTGATGAAATTGCCAGGTTGTCTGATATTTCTTTCAGACTTCGCCAGACAGATTGCTGATAAT; AAATTAGGTAAGATAATTTGTTGGGCCATATTTTAGGACAGGTAAAATAACATCAGGTTCCAGTTGCTT; GAATTGCAAGGCTAAGAAGTACTGCCCTTTTGTGTGTTAGCAGTCAAATCTATTATTCCACTGGCGCAT; CATATGCAGTGATATATGCCTATAATATAAGCCATAGGTTCACACCATTTTGTTTAGACAATTGTCTTT; TTTTCAAGATGCTTTGTTTCTTTCATATGAAAAAAATGCATTTTATAAATTCAGAAAGTCATAGATTTC; TGAAGGCGTCAACGTGCATTTTATTTATGGACTGGTAAGTAACTGTGGTTTACTAGCAGGAATATTTCC; AATTTCTACCTTTACTACATCTTTTCAACAAGTAACTTTGTAGAAATGAGCCAGAAGCCAAGGCCCTGA; GTTGGCAGTGGCCCATAAGTGTAAAATAAAAGTTTACAGAAACCTTAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001957 unedited; GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCTGACGATTGTGGAGA; GGCGGTGGAGAGGCTTCATCCATCCCACCCGGTCGTCGCCGGGGATTGGGGTCCCAGCGA; GACCTCCCCGGGAGAAGCAGTGCCCAGGAGGTTTTCTGAAGCCGGGGAAGCTGTGCAGCC; GAAGCCGCCGCCGCGCCGGAGCCCGGGACACCGGCCACCCTCCGCGCCACCCACCCTCGC; CGGCTCCGGCTTCCTCTGGCCCAGGCGCCGCGCGGACCCGGCAGCTGTCTGCGCACGCCG; AGCTCCACGGTGAAAAAAAAGTGAAGGTGTAAAAGCAGCACAAGTGCAATAAGAGATATT; TCCTCAAATTTGCCTCAAGATGGAAACCCTTTGCCTCAGGGCATCCTTTTGGCTGGCACT; GGTTGGATGTGTAATCAGTGATAATCCTGAGAGATACAGCACAAATCTAAGCAATCATGT; GGATGATTTCACCACTTTTCGTGGCACAGAGCTCAGCTTCCTGGTTACCACTCATCAACC; CACTAATTTGGTCCTACCCAGCAATGGCTCAATGCACAACTATTGCCCACAGCAGACTAA; AATTACTTCAGCTTTCAAATACATTAACACTGTGATATCTTGTACTATCCTCATCGTGGG; AATGGTGGTGAATGCAACTCTGCTCAGGATCATTTACCAGAACACATGTATGAGGAATGG; CCCCAACGCGCTGGATACCAGTCTTGCCCTTGGAGACCTTATCTATGCGGCCATTGATCT; CCCTATCAATGTATCTAAGCTGCTGGGCTGGCCGCTGGGCCTTTTGATCACAATGACTTT; TGCCGTATTTCTTAGCAGGCTGGTTCCCCCTTTTGCACAAGTCCTCTGTGGGGGATCACC; GGTCCTCACCTCTGCGCTCTTAGTGTTNGACAGGGACAANACCAATTGCCTCCTGCAGTC; GCGGTTAGGGAATTGGGATTCTTNTGTAACTGCCTCGAATCGCCTCCATTGCACCTGTTC; TTTTCCTGGCCTTCTGAGCANGGCTNTGAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001957 unedited; NTTTAGCTCTGNACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGGGTTCTGGAAACTTTTATTTTACAC; TTATGGGCCACTGCCAACTCAGGGCCTTGGTTTTTGGCTCATTTTTACAAAGTTACTTGT; TGAAAAAATGTAATAAAGGTAAAAATTGGAAATTTTCCTGCTAGTAAACCACAGTTACTT; ACCAGTCCATAAATAAAATGCCCGTTGACCCCTTCAAAAATCTATGACTTTTTGAATTTA; TAAAAGGCATTTTTTTCATATGAAAAAAACAAAGCCTTTTGAAAAAAAAACAATTGTCTA; AACAAAAGGGGGGGAACCTATGGCTTATATTATAGGCATATATCACTGCATATGATGCCC; CAGGGGAATAATAAATTTGACTGCTAACACACAAAAGGGCAGTACTTTTTAACCTTGCAA; TTCAAGCAACTGGAACCTGATGTTATTTTACCTGGCCTAAAATATGGCCCAACAAATTAT; CTTACCTAATTTATTATCACCAATCTGTCTGGCGAAGTCTGAAAAAAATATCAAACAACC; TGGCAATTTCATCCCCACATTGAATTGGGGATAAAAACAATTTAATTCTTCAAAGGGTAA; TGACTTTTGCTCCACCCCAGGAAACAATTTGACCCCAAATACTGCTTTTTTAAACCAACC; AACCAAACAAAACCTATATTGTTAGTCCACAGCTCATCTGTTTTCACCCTGCCTTTTAAT; TCCATTGTGAATGGGACCCTGATACCAGATTTTTGAATGCCAAAGGTTGCTACACTTTAA; AATGGCCCTGAGCCGGTTGTCTTGATGAACATCCCCTTAAATTTACTCAAACTTTGCCCT; GTGGCACAACTGCGGNGCCCAATTTTNAATATAAATAAACCCCTCTCCTCCCCTNCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_001957
• Insert Size: 4041 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001957.1
• RefSeq Size: 4105 bp
• RefSeq ORF: 4041 bp
• Locus ID: 1909
• UniProt ID: P25101
• Cytogenetics: 4q31.22-q31.23
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Vascular smooth muscle contraction
• MW: 154.8 kDa
• Summary: This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]; Transcript Variant: This variant (1, also known as ET-AR) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.