GFPT1 (NM_002056) Human Untagged Clone

CAT#: SC118874

GFPT1 (untagged)-Human glutamine--fructose-6-phosphate transaminase 1 (GFPT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GFPT1
• Synonyms: CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1; GFAT1m; GFPT; GFPT1L; MSLG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118874 sequence for NM_002056 edited (data generated by NextGen Sequencing)
  ATGTGTGGTATATTTGCTTACTTAAACTACCATGTTCCTCGAACGAGACGAGAAATCCTG
  GAGACCCTAATCAAAGGCCTTCAGAGACTGGAGTACAGAGGATATGATTCTGCTGGTGTG
  GGATTTGATGGAGGCAATGATAAAGATTGGGAAGCCAATGCCTGCAAAATCCAGCTTATT
  AAGAAGAAAGGAAAAGTTAAGGCACTGGATGAAGAAGTTCACAAGCAACAAGATATGGAT
  TTGGATATAGAATTTGATGTACACCTTGGAATAGCTCATACCCGTTGGGCAACACATGGA
  GAACCCAGTCCTGTCAATAGCCACCCCCAGCGCTCTGATAAAAATAATGAATTTATCGTT
  ATTCACAATGGAATCATCACCAACTACAAAGACTTGAAAAAGTTTTTGGAAAGCAAAGGC
  TATGACTTCGAATCTGAAACAGACACAGAGACAATTGCCAAGCTCGTTAAGTATATGTAT
  GACAATCGGGAAAGTCAAGATACCAGCTTTACTACCTTGGTGGAGAGAGTTATCCAACAA
  TTGGAAGGTGCTTTTGCACTTGTGTTTAAAAGTGTTCATTTTCCCGGGCAAGCAGTTGGC
  ACAAGGCGAGGTAGCCCTCTGTTGATTGGTGTACGGAGTGAACATAAACTTTCTACTGAT
  CACATTCCTATACTCTACAGAACAGGCAAAGACAAGAAAGGAAGCTGCAATCTCTCTCGT
  GTGGACAGCACAACCTGCCTTTTCCCGGTGGAAGAAAAAGCAGTGGAGTATTACTTTGCT
  TCTGATGCAAGTGCTGTCATAGAACACACCAATCGCGTCATCTTTCTGGAAGATGATGAT
  GTTGCAGCAGTAGTGGATGGACGTCTTTCTATCCATCGAATTAAACGAACTGCAGGAGAT
  CACCCCGGACGAGCTGTGCAAACACTCCAGATGGAACTCCAGCAGATCATGAAGGGCAAC
  TTCAGTTCATTTATGCAGAAGGAAATATTTGAGCAGCCAGAGTCTGTCGTGAACACAATG
  AGAGGAAGAGTCAACTTTGATGACTATACTGTGAATTTGGGTGGTTTGAAGGATCACATA
  AAGGAGATCCAGAGATGCCGGCGTTTGATTCTTATTGCTTGTGGAACAAGTTACCATGCT
  GGTGTAGCAACACGTCAAGTTCTTGAGGAGCTGACTGAGTTGCCTGTGATGGTGGAACTA
  GCAAGTGACTTCCTGGACAGAAACACACCAGTCTTTCGAGATGATGTTTGCTTTTTCCTT
  AGTCAATCAGGTGAGACAGCAGATACTTTGATGGGTCTTCGTTACTGTAAGGAGAGAGGA
  GCTTTAACTGTGGGGATCACAAACACAGTTGGCAGTTCCATATCACGGGAGACAGATTGT
  GGAGTTCATATTAATGCTGGTCCTGAGATTGGTGTGGCCAGTACAAAGGCTTATACCAGC
  CAGTTTGTATCCCTTGTGATGTTTGCCCTTATGATGTGTGATGATCGGATCTCCATGCAA
  GAAAGACGCAAAGAGATCATGCTTGGATTGAAACGGCTGCCTGATTTGATTAAGGAAGTA
  CTGAGCATGGATGACGAAATTCAGAAACTAGCAACAGAACTTTATCATCAGAAGTCAGTT
  CTGATAATGGGACGAGGCTATCATTATGCTACTTGTCTTGAAGGGGCACTGAAAATCAAA
  GAAATTACTTATATGCACTCTGAAGGCATCCTTGCTGGTGAATTGAAACATGGCCCTCTG
  GCTTTGGTGGATAAATTGATGCCTGTGATCATGATCATCATGAGAGATCACACTTATGCC
  AAGTGTCAGAATGCTCTTCAGCAAGTGGTTGCTCGGCAGGGGCGGCCTGTGGTAATTTGT
  GATAAGGAGGATACTGAGACCATTAAGAACACAAAAAGAACGATCAAGGTGCCCCACTCA
  GTGGACTGCTTGCAGGGCATTCTCAGCGTGATCCCTTTACAGTTGCTGGCTTTCCACCTT
  GCTGTGCTGAGAGGCTATGATGTTGATTTCCCACGGAATCTTGCCAAATCTGTGACTGTA
  GAGTGA
  
  Clone variation with respect to NM_002056.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002056 unedited
  CACTCGGNGATTTGTNAAACCGACTTACTNATAGGNNCGGCCGCGCATTCGGCACGAGGC
  GGAGCCCGGGAGGCGGAGAAGGCTGTCGTTGCCTTGGCCGTCGCATCCCCGAGGGAGTCG
  TGTCGGCGCCACCCCGGCCCCCGAGCCCGCAGATTGCCCACCGAAGCTCGTGTGTGCACC
  CCCGATCCCGCCAGCCACTCGCCCCTGGCCTCGCGGGCCGTGTCTCCGGCATCATGTGTG
  GTATATTTGCTTACTTAAACTACCATGTTCCTCGAACGAGACGAGAAATCCTGGAGACCC
  TAATCAAAGGCCTTCAGAGACTGGAGTACAGAGGATATGATTCTGCTGGTGTGGGATTTG
  ATGGAGGCAATGATAAAGATTGGGAAGCCAATGCCTGCAAAATCCAGCTTATTAAGAAGA
  AAGGAAAAGTTAAGGCACTGGATGAAGAAGTTCACAAGCAACAAGATATGGATTTGGATA
  TAGAATTTGATGTACACCTTGGAATAGCTCATACCCGTTGGGCAACACATGGAGAACCCA
  GTCCTGTCAATAGCCACCCCCAGCGCTCTGATAAAAATAATGAATTTATCGTTATTCACA
  ATGGAATCATCACCAACTACAAAGACTTGAAAAAGTTTTTGGAAAGCAAAGGCTATGACT
  TCGAATCTGANACAGACACAGAGACAATTGCCAAGCTCGTTAAGTATATGTATGACAATC
  GGGAAAGTCAAGATACCAGCTTTACTACCTTGGTGGAGAGAGTTATCCAACAATTGGAAG
  GTGCTTTTGCACTTGTGTTTAAAGTGTTCATTTTCCCGGGCAAGCAGTTGGCACAAGGCG
  AGGTAGCCCTCTGTTGATTGGTGTACGGAGTGAACATAAACTTTCTACTGATCACATTCC
  TATACTCTACAGAACAGGCAAAGACAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002056 unedited
  GGGGGGGTGGTTTNACAANNAAAATATAAAACCNGGGTGTTCTGTTNATTTNATAANCGT
  TCTGTGTTGTNGATTTAAANAACAGTAAAAATTGTCATTCAGCAGTCATTTTAAAAAATA
  AAAAACTACAGATACAAGGAAATAAAAAACACTTTTAGGAGATGAAAACACAAAGTAGTA
  GGATACCCAACATGTACACATCCCATCTTCAAATTTAAAATCATATTGTCAGTTGTCCAA
  AGCAGCTTGAATTTAAAGTTTGTGCTATAAAATTGTGCAAATATGTTAAGGATTGAGACC
  CACCAATGCACTACTGTAATATTTCGCTTCCTAAATTTCTTCCACCTACAGATAATAGAC
  AACAAGTCTGAGAAACTAAGGCTAACCAAACTTAGATATAAATCCTACCAATAAAATTTT
  TCAGTTTTAAGTTTTACAGTTTGATTTAAAAACAAAACAGAAACAAATTTCAAAATAAAT
  CACATCTTCTCTTAAAACTTGGCAAACCCTTCCCTAACTGTCCAAGTATGAGCATACACT
  GCCACTGGCTTTAGATACTCCAATTAAATGCACTACTCTTTCACTGGTCTGAATGAAGTA
  TGGTGAAACAAGTACCAATTTTTTAAAACATATTGTCTTTTTAAATTGAAGGGGAGGGGT
  CCAAAAATGGACAACCCAAATTTTTTTAAAAAAAAAAAAAGCTTAAAAATTTTTTTTGAT
  AACAAATTCCCAAATTGAAATTCCTTTTTTTTAAAAAGTTTTCCAACAATTTTCTGGGGA
  AAAATTAAAAGGGGGTTCTTTCAACAAAAAACAAAATTTTTTGGGGGGAATAAATTTAAC
  ACGGGGTGTTTTAAAAAAAGGGGTGGTCTTCAAAAAAAAGGCTCTTGAGGGGGGGGTTTT
  TTTTAAAAAAAGGGGTTTAATAAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002056
• Insert Size: 3460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002056.1, NP_002047.1
• RefSeq Size: 8649 bp
• RefSeq ORF: 2046 bp
• Locus ID: 2673
• UniProt ID: Q06210
• Cytogenetics: 2p13.3
• Domains: GATase_2, SIS
• Protein Families: Protease
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Amino sugar and nucleotide sugar metabolism, Metabolic pathways
• Gene Summary: This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. [provided by RefSeq, Sep 2008]
  Transcript Variant: This variant (2) lacks an alternate exon in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.