TFIIE beta (GTF2E2) (NM_002095) Human Untagged Clone

SKU SC118828

GTF2E2 (untagged)-Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TFIIE beta
• Synonyms: FE; TF2E2; TFIIE-B; TTD6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002095, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGATCCAAGCCTGTTGAGAGAAAGGGAGCTGTTCAAAAAACGAGCTCTTTCTACTCCTGTAGTAGAAA; AACGTTCAGCATCTTCTGAGTCATCATCATCATCGTCAAAGAAGAAGAAAACAAAGGTAGAACATGGAGG; ATCGTCAGGCTCTAAACAAAATTCTGATCATAGCAATGGATCATTTAACTTGAAAGCTTTGTCAGGAAGC; TCTGGATATAAGTTTGGTGTTCTTGCTAAGATTGTGAATTACATGAAGACACGGCATCAGCGAGGAGATA; CGCATCCTCTAACCTTAGATGAAATTTTGGATGAAACACAACATTTAGATATTGGACTCAAGCAGAAACA; ATGGCTAATGACTGAGGCTTTAGTCAACAATCCCAAAATTGAAGTAATAGATGGGAAGTATGCTTTCAAG; CCCAAGTACAACGTGAGAGATAAGAAGGCCCTACTTAGGCTCTTAGATCAGCATGACCAGCGAGGATTAG; GAGGAATTCTTTTAGAAGACATAGAAGAAGCACTGCCCAATTCCCAGAAAGCTGTCAAGGCTTTGGGGGA; CCAGATACTATTTGTAAATCGTCCCGATAAGAAGAAAATACTTTTCTTCAATGATAAGAGCTGTCAGTTT; TCTGTGGATGAAGAATTTCAGAAACTGTGGAGGAGTGTCACTGTAGATTCCATGGACGAGGAGAAAATTG; AAGAATATCTGAAGCGACAGGGTATTTCTTCCATGCAGGAATCTGGACCAAAGAAAGTGGCCCCTATTCA; GAGAAGGAAAAAGCCTGCTTCACAGAAAAAGCGACGCTTTAAGACTCATAACGAACACTTGGCTGGAGTG; CTGAAGGATTACTCTGACATTACTTCCAGCAAATAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002095 unedited; TATATTTGTATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCTAGCACCCGA; TCGGGAAGTGGCGGGCGGAGTCCCGGGTCCAGTCGCCGCCTCAGCTACCGCCGCTGCCGC; CGCCGCCGCCGCCACCGCCAGTGGTGAGACCCCGACCTGGCGGGTCAGCGCTGGGCGTGC; GTGCGGGCAGGCGGGGGCGCTGACGAGAAGCAGGAAGAGGGTGCAGTGCCGGCGTGGGCG; GCCGGCCGAGGCGGAGGCGCAGGAAGGGGGCGGCGAGTCGTGCGAGGCTGCCCTTCTCAC; TCAGCATTATGGATCCAAGCCTGTTGAGAGAAAGGGAGCTGTTCAAAAAACGAGCTCTTT; CTACTCCTGTAGTAGAAAAACGTTCAGCATCTTCTGAGTCATCATCATCATCGTCAAAGA; AGAAGAAAACAAAGGTAGAACATGGAGGATCGTCAGGCTCTAAACAAAATTCTGATCATA; GCAATGGATCATTTAACTTGAAAGCTTTGTCAGGAAGCTCTGGATATAAGTTTGGTGTTC; TTGCTAAGATTGTGAATTACATGAAGACACGGCATCAGCGAGGAGATACGCATCCTCTAA; CCTTAGATGAAATTTTGGATGAAACACAACATTTAGATATTGGACTCAAGCAGAAACAAT; GGCTAATGACTGAGGCTTTAGTCAACAATCCCAAAATTGAAGTAATAGATGGGAAGTATG; CTTTCAAGCCCAAGTACAACGTGAGAGATAAGAAGGCCCTACTTANGCTCTTAGATCAGC; ATGACCAGCGAGGATTAGGGAGGATTCTTTTAGAAGACATAGAAGAAGCACTGCCCAATT; CCCAGAAGCTGTCAGGGCTTTGGGGACAGATCTATTGTAATCGTCCGATAGAAGAAATAC; TTTCTCATGATAAGCTGTCAGTTCTGTGATGAAGATTCAGACTGGGAGGATGTACTGTAA; TCCTGGCGAGGAGAATGAGAATTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002095 unedited; NNNTTTTTCCTATGGACCGCGGGCCGCATTCTAGGATCGAGTTTTTTTTTTTTTTTTTTT; TTTTTTTCCTTCTACAGGGATTTTATTATAAATATGTTCTTTTGGAATAAACAAATATTG; CATTCAAGACTATGGGCAAACTTGCTCTAAAGTAACAAACGAGGAAGAAAGGAACCAGGA; ACCATTTAAAAGTGGAGAAAAACACAGCAAAGACACCTGCATGCCACGCTCAGCGCCTTT; CTCCCAGGGAGTAACAGAAGGTTAACAGGGAACATCACATTGCCCATGAGCCCATTCTAC; TCAAATAAGCTTTGAGTTTGGTAATTTGCACTTGTTTTGTAAACTGAACTGCTCCTCTCC; TCAGCCGCAAGAAGCAGATAGGAAGACAGTCTTCAGACCCCGAGCATCAGCAAGAACACT; CTTGATTGGGTATCTGTAACTCTGTTCCAGGGCAAAACTGTTCCCTATTTGCTGGAAGTA; ATGTCAGAGTAATCCTTCAGCACTCCAGCCAAGTGTTCGTTATGAGTCTTAAAGCGTCGC; TTTTTCTGTGAAGCAGGCTTTTTCCTTCTCTGAATAGGGGCCACTTTCTTTGGTCCAGAT; TCCTGCATGGAAGAAATACCCTGTCGCTTCAGATATTCTTCAATTTTCTCCTCGTCCATG; GAATCTACAGTGACACTCCTCCACAGTTTCTGAAATTCTTCATCCACAGAAAACTGACAG; CTCTTATCATTGAAGAAAAGTATTTTCTTCTTATCGGGACGATTTACAAATAGTATCTGG; TCCCCCCAAGCCTTGACAGCTTTCTGGGAATTGGGCAGTGCTTCTTCTATTGTCTCTAAA; GAATTCTCCTAACCTCGCTGGCATGCTGATCTAAGAGCCTAGTAGGGCCTTCTTATCTCT; CACGTGTACTTGGGCTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002095
• Insert Size: 1640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002095.3, NP_002086.1
• RefSeq Size: 1560 bp
• RefSeq ORF: 1560 bp
• Locus ID: 2961
• UniProt ID: P29084
• Cytogenetics: 8p12
• Domains: TFIIE_beta
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Summary: The general transcription factor IIE (TFIIE) is part of the RNA polymerase II transcription initiation complex, recruiting TFIIH and being essential for promoter clearance by RNA polymerase II. TFIIE is a heterodimer (and sometimes heterotetramer) of alpha and beta subunits. The protein encoded by this gene represents the beta subunit of TFIIE. [provided by RefSeq, Jan 2017]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).