Aspartate Aminotransferase (GOT1) (NM_002079) Human Untagged Clone

SKU SC118816

GOT1 (untagged)-Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Aspartate Aminotransferase
• Synonyms: AST1; ASTQTL1; cAspAT; cCAT; GIG18
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118816 sequence for NM_002079 edited (data generated by NextGen Sequencing); ATGGCACCTCCGTCAGTCTTTGCCGAGGTTCCGCAGGCCCAGCCTGTCCTGGTCTTCAAG; CTCACTGCCGACTTCAGGGAGGATCCGGACCCCCGCAAGGTCAACCTGGGAGTGGGAGCA; TATCGCACGGATGACTGCCATCCCTGGGTTTTGCCAGTAGTGAAGAAAGTGGAGCAGAAG; ATTGCTAATGACAATAGCCTAAATCACGAGTATCTGCCAATCCTGGGCCTGGCTGAGTTC; CGGAGCTGTGCTTCTCGTCTTGCCCTTGGGGATGACAGCCCAGCACTCAAGGAGAAGCGG; GTAGGAGGTGTGCAATCTTTGGGGGGAACAGGTGCACTTCGAATTGGAGCTGATTTCTTA; GCGCGTTGGTACAATGGAACAAACAACAAGAACACACCTGTCTATGTGTCCTCACCAACC; TGGGAGAATCACAATGCTGTGTTTTCCGCTGCTGGTTTTAAAGACATTCGGTCCTATCGC; TACTGGGATGCAGAGAAGAGAGGATTGGACCTCCAGGGCTTCCTGAATGATCTGGAGAAT; GCTCCTGAGTTCTCCATTGTTGTCCTCCACGCCTGTGCACACAACCCAACTGGGATTGAC; CCAACTCCGGAGCAGTGGAAGCAGATTGCTTCTGTCATGAAGCACCGGTTTCTGTTCCCC; TTCTTTGACTCAGCCTATCAGGGCTTCGCATCTGGAAACCTGGAGAGAGATGCCTGGGCC; ATTCGCTATTTTGTGTCTGAAGGCTTCGAGTTCTTCTGTGCCCAGTCCTTCTCCAAGAAC; TTCGGGCTCTACAATGAGAGAGTCGGGAATCTGACTGTGGTTGGAAAAGAACCTGAGAGC; ATCCTGCAAGTCCTTTCCCAGATGGAGAAGATCGTGCGGATTACTTGGTCCAATCCCCCC; GCCCAGGGAGCACGAATTGTGGCCAGCACCCTCTCTAACCCTGAGCTCTTTGAGGAATGG; ACAGGTAATGTGAAGACAATGGCTGACCGGATTCTGACCATGAGATCTGAACTCAGGGCA; CGACTAGAAGCCCTCAAAACCCCTGGGACCTGGAACCACATCACTGATCAAATTGGCATG; TTCAGCTTCACTGGGTTGAACCCCAAGCAGGTTGAGTATCTGGTCAATGAAAAGCACATC; TACCTGCTGCCAAGTGGTCGAATCAACGTGAGTGGCTTAACCACCAAAAATCTAGATTAC; GTGGCCACCTCCATCCATGAAGCAGTCACCAAAATCCAGTGA; ; Clone variation with respect to NM_002079.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002079 unedited; ACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGCCAGACCTGACCTGCCAGCTCCGGG; CGTGGGGTGAAATCTCTTGATTCCTAGTCTCTCGATATGGCACCTCCGTCAGTCTTTGCC; GAGGTTCCGCAGGCCCAGCCTGTCCTGGTCTTCAAGCTCACTGCCGACTTCAGGGAGGAT; CCGGACCCCCGCAAGGTCAACCTGGGAGTGGGAGCATATCGCACGGATGACTGCCATCCC; TGGGTTTTGCCAGTAGTGAAGAAAGTGGAGCAGAAGATTGCTAATGACAATAGCCTAAAT; CACGAGTATCTGCCAATCCTGGGCCTGGCTGAGTTCCGGAGCTGTGCTTCTCGTCTTGCC; CTTGGGGATGACAGCCCAGCACTCAAGGAGAAGCGGGTAGGAGGTGTGCAATCTTTGGGG; GGAACAGGTGCACTTCGAATTGGAGCTGATTTCTTAGCGCGTTGGTACAATGGAACAAAC; AACAAGAACACACCTGTCTATGTGTCCTCACCAACCTGNGAGAATCACAATGCTGTGTTT; TCCGCTGCTGGTTNTAAAGACATTCGGTCCTATCGCTACTGNGATGCAGAGAGAGAGGAT; TGGACCTCCAGGGCTTCCTGAATGATCTGGAGAATGCTCCTGAGTTCTCCATTGNTGTCC; TCCACGCCTGTGCACACCACCCAACTGGNGATGACCNCACTCNGNAGCAGTGGAAGCAGA; TTGCTTCTGTCATGAAGCACCGTTTNNCTGTNCCCTTCTTTGACTCANCTATCAGGNCTT; CGCATCTGGAAACCCTGAGAGAGATGCCCTGGCCATTCGCTATTTTGTGTCTGAAGGCTT; CAGTTCTTCTGTGCCCAGTCCTCTNCAGAACTNCNGGCTCTACATGANAGAGTTCGGAAT; CTGACTGTGGGTGGAAAAGACCTGANAGCTNCTTGCAGGTCCTTCCCAATGGGAAAGATC; GTGCGATTACTTGTTCCATNCCCCGCCAGGAGACAGATTGGGGCCGACCCTCCTAACCTG; ACCTTTGGGAAGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002079 unedited; AAACCCCCGGCCCNCCCTAACTCCTATATCCCCTGCTTTTACTTGANCCGCGGCACGCTA; TTCTANATCGAGTTTTTTTTTTTTTTTTTTGCACAACATTTTTATTTTATTTTTTTAGGC; GAAGTACAACACAATACAATGGCTCAAAAATATCAGAATGCACTACGCACATCACGAGTA; AATACTGTCTGGTAAAACTTGTTTCAGTTAAATATGTACGTGCCCGCGCATGTCATGATT; AAATATCCTTCTTACCACAGTCACCCTAAAGAACCAAAGCTTAGGACTAGGGACACACCC; ATGCAGAAAGAGCAGGGAGACCAGACACTCTGGGTTGAGATGATGAATTTAATGCCGCAG; CCGACACCCACATTCACACTTTGGCTCCTTTACACAACTTGATCTTTGGGACTACCAATT; TGTACAGAGTTAAACACCACATAGAAGCACGCGGCCGCCACACACAACACTCCTTTTTAG; TGAGAGGGACACCACCCCTATGTCTCATGATCCAAGCACTCTTTTATTCTTAAATAAAAA; TCTTAATTTGCCTTTGCACCTCAAAGGCTCTAATCCCAGTCTTCAAATTCGTCTCAAGGG; ATGTTCTCTTCATGTGGGGCTCGGTCCAAACCGCACGCTGCCTTACCACACCCGCCTTTC; ACCTCCTCGCAGCGTCCCTCTACCCCCATGGCCATGTCCATGCACGTTTCGTCCCGCCAG; CGACCCCCCCTTAACAGAAACTTCCTTTGGGGCCACTGGACCGGGTGGAGTTCCCTTCCC; CTGCATTCTCGGTCACCTGTCTTTATGGCCTGGAGAGTGCCACCCCCACACTCCAATCTT; TCCGGGCCAAACCCACTTTACGCCCGATTTCCACACCTCGCCAACCCCTTCCAATTCCTC; TTTTACTTGGCTCAAAACTTACAACCGCTTCGGGGTTCACACCAAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002079
• Insert Size: 2080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002079.1, NP_002070.1
• RefSeq Size: 1941 bp
• RefSeq ORF: 1242 bp
• Locus ID: 2805
• UniProt ID: P17174
• Cytogenetics: 10q24.2
• Domains: aminotran_1_2
• Protein Pathways: Alanine, Arginine and proline metabolism, aspartate and glutamate metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, Phenylalanine metabolism, tyrosine and tryptophan biosynthesis, Tyrosine metabolism
• Summary: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]