HLADQA1 (HLA-DQA1) (NM_002122) Human Untagged Clone

SKU SC118795

HLA (untagged)-Human major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HLADQA1
• Synonyms: CELIAC1; DQ-A1; DQA1; HLA-DQA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118795 sequence for NM_002122 edited (data generated by NextGen Sequencing); ATGATCCTAAACAAAGCTCTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCC; TGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACCAG; TTTTACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTG; GACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGAGTTCAGCAAATTTGGAGGTTTT; GACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATT; AAACGCTACAACTCTACCGCTGCTACCAATGAGGTTCCTGAGGTCACAGTGTTTTCCAAG; TCTCCCGTGACACTGGGTCAGCCCAACACCCTCATCTGTCTTGTGGACAACATCTTTCCT; CCTGTGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAG; ACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAAGATCAGTTACCTCACCTTCCTC; CCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTT; CTGAAACACTGGGAGCCTGAGATTCCAGCCCCTATGTCAGAGCTCACAGAGACTGTGGTC; TGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGCACTGTCTTCATCATC; CAAGGCCTGCGTTCAGTTGGTGCTTCCAGACACCAAGGGCCATTGTGA; ; Clone variation with respect to NM_002122.3; 396 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002122 unedited; CGGTCAGCATTTGTATACGATTCACTATAGGCGGCCGCGAATTCGCACGAGGCCAACTGC; TGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCTCTGCTGCTGGGGGCCCTCGCT; CTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCT; TGTGGTGTAAACTTGTACCAGTTTTACGGTCCCTCTGGCCAGTACACCCATGAATTTGAT; GGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGAG; TTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAA; CACAACTTGAACATCATGATTAAACGCTACAACTCTACCGCTGCTACCAATGAGGTTCCT; GAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATCTGT; CTTGTGGACAACATCTTTCCTCCTGTGGTCAACATCACATGGCTGAGCAATGGGCAGTCA; GTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAAG; ATCAGTTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCAC; TGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGAGCCTGAGATTCCAGCCCCTATGTCA; GAGCTCACAGAGACTGTGGTCTGTGCCCTGNGGTTGTCTGTGGGCCTCATGGGCATTGTG; GTGGGCACTGTCTTCATCATCCAAGGCCTGCGTTCAGTTGGTGCTTCCAGACACCAAGGG; CCATTGTGAATCCATCCTGAANGGAAGGTGCATCGCCATCTACAGGAGCAGAAGAATGGA; CTTGCTAAATGAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002122 unedited; ATGGACCGCGCGCCGCATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTCATAAAAGGA; ATTTATTGCTTCCTTGGAGATTTTAGATGGAGGTTACTGAGGAATTAGGTAGCCGGGTGG; CTTACCCCAGGCATGTTTTGTAGGTAACATTTGAGAAAAGAAAAAAAAAATCAGGAGGTC; AGGGAAAGAATGTAAAGGCATTTGTGAGCTCTGAGGGGATATAGCAGCTTAAGTCCCAGA; GAAAAGGTGAGAGGAGAAATATTTGGAGGAGAAAAGGGATATGATAAATCGGGCCAGAGA; ATAGTGCTAGGTCATTTAGCAAGTCCATTCTTCTGCTCCTGTAGATGGCGATGCACCTTC; CCTTCCAGGATGGGATTCACAATGGCCCTTGGTGTCTGGAAGCACCAACTGAACGCAGGC; CTTGGATGATGAAGACAGTGCCCACCACAATGCCCATGAGGCCCACAGACAACCCCAGGG; CACAGACCACAGTCTCTGTGAGCTCTGACATAGGGGCTGGAATCTCAGGCTCCCAGTGTT; TCAGAAGAGGCTGGTCCAGGCCCCAGTGCTCCACCTTGCAGTCATAAATCTCATCAGCAG; AAGGGAGGAAGGTGAGGTAACTGATCTTGAAGAAGGAATGATCACTCTTGGAGAGGAAGC; TGGTCTCAGAAACACCTTCTGTGACTGACTGCCCATTGCTCAGCCATGTGATGTTGACCA; CAGGAGGAAAGATGTTGTCCACAAGACAGATGAGGGTGTTGGGCTGACCCAGTGTCACGG; GAGACTTGGAAAACACTGTGACCTCAGGAACCTCATTGGTAGCAGCGGTAGAGNTGTAGC; GTTTAATCATGATGTTAAAGTGTGTTTTGCCACACCCATGTTCTCAGTGCACCCTGCGGG; TCAAACCTCCAATTNGCTGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_002122
• Insert Size: 1240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002122.3, NP_002113.2
• RefSeq Size: 1542 bp
• RefSeq ORF: 768 bp
• Locus ID: 3117
• UniProt ID: P01909
• Cytogenetics: 6p21.32
• Domains: ig, IGc1, MHC_II_alpha
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
• Summary: HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq, Jul 2008]