Cytokeratin 8 (KRT8) (NM_002273) Human Untagged Clone

CAT#: SC118727

KRT8 (untagged)-Human keratin 8 (KRT8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytokeratin 8
• Synonyms: CARD2; CK-8; CK8; CYK8; K2C8; K8; KO
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118727 sequence for NM_002273 edited (data generated by NextGen Sequencing)
  ATGTCCATCAGGGTGACCCAGAAGTCCTACAAGGTGTCCACCTCTGGCCCCCGGGCCTTC
  AGCAGCCGCTCCTACACGAGTGGGCCCGGTTCCCGCATCAGCTCCTCGAGCTTCTCCCGA
  GTGGGCAGCAGCAACTTTCGCGGTGGCCTGGGCGGCGGCTATGGTGGGGCCAGCGGCATG
  GGAGGCATCACCGCAGTTACGGTCAACCAGAGCCTGCTGAGCCCCCTTGTCCTGGAGGTG
  GACCCCAACATCCAGGCCGTGCGCACCCAGGAGAAGGAGCAGATCAAGACCCTCAACAAC
  AAGTTTGCCTCCTTCATAGACAAGGTACGGTTCCTGGAGCAGCAGAACAAGATGCTGGAG
  ACCAAGTGGAGCCTCCTGCAGCAGCAGAAGACGGCTCGAAGCAACATGGACAACATGTTC
  GAGAGCTACATCAACAACCTTAGGCGGCAGCTGGAGACTCTGGGCCAGGAGAAGCTGAAG
  CTGGAGGCGGAGCTTGGCAACATGCAGGGGCTGGTGGAGGACTTCAAGAACAAGTATGAG
  GATGAGATCAATAAGCGTACAGAGATGGAGAACGAATTTGTCCTCATCAAGAAGGATGTG
  GATGAAGCTTACATGAACAAGGTAGAGCTGGAGTCTCGCCTGGAAGGGCTGACCGACGAG
  ATCAACTTCCTCAGGCAGCTGTATGAAGAGGAGATCCGGGAGCTGCAGTCCCAGATCTCG
  GACACATCTGTGGTGCTGTCCATGGACAACAGCCGCTCCCTGGACATGGACAGCATCATT
  GCTGAGGTCAAGGCACAGTACGAGGATATTGCCAACCGCAGCCGGGCTGAGGCTGAGAGC
  ATGTACCAGATCAAGTATGAGGAGCTGCAGAGCCTGGCTGGGAAGCACGGGGATGACCTG
  CGGCGCACAAAGACTGAGATCTCTGAGATGAACCGGAACATCAGCCGGCTCCAGGCTGAG
  ATTGAGGGCCTCAAAGGCCAGAGGGCTTCCCTGGAGGCCGCCATTGCAGATGCCGAGCAG
  CGTGGAGAGCTGGCCATTAAGGATGCCAACGCCAAGTTGTCCGAGCTGGAGGCCGCCCTG
  CAGCGGGCCAAGCAGGACATGGCGCGGCAGCTGCGTGAGTACCAGGAGCTGATGAACGTC
  AAGCTGGCCCTGGACATCGAGATCGCCACCTACAGGAAGCTGCTGGAGGGCGAGGAGAGC
  CGGCTGGAGTCTGGGATGCAGAACATGAGTATTCATACGAAGACCACCAGCGGCTATGCA
  GGTGGTCTGAGCTCGGCCTATGGGGGCCTCACAAGCCCCGGCCTCAGCTACAGCCTGGGC
  TCCAGCTTTGGCTCTGGCGCGGGCTCCAGCTCCTTCAGCCGCACCAGCTCCTCCAGGGCC
  GTGGTTGTGAAGAAGATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTC
  CTGCCCAAGTGA
  
  Clone variation with respect to NM_002273.3
  681 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002273 unedited
  ACGGTCACGATTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCTTCTC
  CGCTCCTTCTAGGATCTCCGCCTGGTTCGGCCCGCCTGCCTCCACTCCTGCCTCCACCAT
  GTCCATCAGGGTGACCCAGAAGTCCTACAAGGTGTCCACCTCTGGCCCCCGGGCCTTCAG
  CAGCCGCTCCTACACGAGTGGGCCCGGTTCCCGCATCAGCTCCTCGAGCTTCTCCCGAGT
  GGGCAGCAGCAACTTTCGCGGTGGCCTGGGCGGCGGCTATGGTGGGGCCAGCGGCATGGG
  AGGCATCACCGCAGTTACGGTCAACCAGAGCCTGCTGAGCCCCCTTGTCCTGGAGGTGGA
  CCCCAACATCCAGGCCGTGCGCACCCAGGAGAAGGAGCAGATCAAGACCCTCAACAACAA
  GTTTGCCTCCTTCATAGACAAGGTACGGTTCCTGGAGCAGCAGAACAAGATGCTGGAGAC
  CAAGTGGAGCCTCCTGCAGCAGCAGAAGACGGCTCGAAGCAACATGGACAACATGTTCGA
  GAGCTACATCAACAACCTTAGGCGGCAGCTGGAGACTCTGGGCCAGGAGAAGCTGAAGCT
  GGAGGCGGAGCTTGGCAACATGCAGGGGCTGGTGGAGGACTTCAAGAACAAGTATGAGGA
  TGAGATCAATAAGCGTACAGAGATGGAGAACGAATTTGNCCTCATCAAGAAAGATGTGGA
  TGAAGCCTACATGAACAAGTAANAGCTGGAGTCTCGCCTGGAAGGCTGACCGACGAGATC
  ACTTCCTCCAGCAGCTGTATGAAGAAGAGATCCGGGNAGCTGCAGTCCCAGATCTCGGCA
  CATCTGTGGTGCTGTCCATGGACACAGCCGTTCCCTGGCATGGACAGCTCATTGCTTAGG
  TAAGCCCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002273 unedited
  AGAGTCGAGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTCGACATTGGCAGAGCTAGCTGA
  GGTTTTATTTTGGACCAAAAAAAAAAAGCAATTGAATTGTTTTGTACCTGGAGGCATGGG
  CAAGGGGGGTCCCCAGGTAGTAAACTCCCCAGGTGGGCTGAGGGCTAAGGCTGAACCTCA
  GGTGGGTCTCCTGTTCCCAGTGCTACCCTGCATAGCGGCCTCCTTCCCAGGCTCTGGGGC
  AACGCAAGAGGGGTAGGCTGGGAGGGGCTGCCCCAACTGTTCACTTGGGCAGGACGTCAG
  AGGACTCAGACACCAGCTTCCCATCACGTGTCTCGATCTTCTTTACAACCACGGCCCTGG
  AGGAACTTGTGCGGCTGAAAGAGCTGGACCCCCCGCCAAAGCCAAAGCTGGACCCCAAGC
  TGTACCTGAAGCCCGGGCTTTTGAGGCCCCCATAGGCCGAGCTTAGACCACCTGCTTAGC
  CCGTGGGGGCATCCACTCTAAAACCTCTGCACCTGCCAACACACTACCAGCGAGCTCCTA
  ACACCGACACACCTCCATTTAGGCCGCCTTTTTTTCTTCCCCGCCCCATTTCCCCTTCCC
  CCTCTCGCTCCCCTCGTCTCCGCCTTCTCCCCCTTACCATCTCCCCCGTCCCCTCACCCC
  GTCTTCCCCCCCCCCGCTTCCCCTCCTTCTTTTTTCCCCTCTTCTCTCTCATTTACCACA
  CTCGTTTTCATTCCAATCTTCCGCCGACCACATATGCCAACCCTCATCTACACATTCCCC
  TACCGCCCGTCCATGCCCATCTCCACCCCCTCGTCGCCTCCCCTCCTCTTCCCCCTTTCA
  CATTCATGTCTTCTCCCGCTCGCGTCATTTCTCCCATTCTATCGCCTTCTCCCACCCCCT
  CTATCTTTCCTTTACCCTTGTTTCCTCCCCTCTCTCTGTTTCCGTTTTCTGCCCCTCCTC
  CCATACCGCGCTCGCGCTTTGCCGTCGCATATTCCACTGTTTCCCTCCTCCTCCCCATAC
  TCTTATACTCCCCCTCACCCTCCTCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002273
• Insert Size: 1880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002273.2, NP_002264.1
• RefSeq Size: 1788 bp
• RefSeq ORF: 1452 bp
• Locus ID: 3856
• UniProt ID: P05787
• Cytogenetics: 12q13.13
• Domains: filament
• Protein Families: Druggable Genome
• Gene Summary: This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation in a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform 2.