LIFR (NM_002310) Human Untagged Clone

SKU SC118708

LIFR (untagged)-Human leukemia inhibitory factor receptor alpha (LIFR), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: LIFR
• Synonyms: CD118; LIF-R; SJS2; STWS; SWS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118708 sequence for NM_002310 edited (data generated by NextGen Sequencing); ATGATGGATATTTACGTATGTTTGAAACGACCATCCTGGATGGTGGACAATAAAAGAATG; AGGACTGCTTCAAATTTCCAGTGGCTGTTATCAACATTTATTCTTCTATATCTAATGAAT; CAAGTAAATAGCCAGAAAAAGGGGGCTCCTCATGATTTGAAGTGTGTAACTAACAATTTG; CAAGTGTGGAACTGTTCTTGGAAAGCACCCTCTGGAACAGGCCGTGGTACTGATTATGAA; GTTTGCATTGAAAACAGGTCCCGTTCTTGTTATCAGTTGGAGAAAACCAGTATTAAAATT; CCAGCTCTTTCACATGGTGATTATGAAATAACAATAAATTCTCTACATGATTTTGGAAGT; TCTACAAGTAAATTCACACTAAATGAACAAAACGTTTCCTTAATTCCAGATACTCCAGAG; ATCTTGAATTTGTCTGCTGATTTCTCAACCTCTACATTATACCTAAAGTGGAACGACAGG; GGTTCAGTTTTTCCACACCGCTCAAATGTTATCTGGGAAATTAAAGTTCTACGTAAAGAG; AGTATGGAGCTCGTAAAATTAGTGACCCACAACACAACTCTGAATGGCAAAGATACACTT; CATCACTGGAGTTGGGCCTCAGATATGCCCTTGGAATGTGCCATTCATTTTGTGGAAATT; AGATGCTACATTGACAATCTTCATTTTTCTGGTCTCGAAGAGTGGAGTGACTGGAGCCCT; GTGAAGAACATTTCTTGGATACCTGATTCTCAGACTAAGGTTTTTCCTCAAGATAAAGTG; ATACTTGTAGGCTCAGACATAACATTTTGTTGTGTGAGTCAAGAAAAAGTGTTATCAGCA; CTGATTGGCCATACAAACTGCCCCTTGATCCATCTTGATGGGGAAAATGTTGCAATCAAG; ATTCGTAATATTTCTGTTTCTGCAAGTAGTGGAACAAATGTAGTTTTTACAACCGAAGAT; AACATATTTGGAACCGTTATTTTTGCTGGATATCCACCAGATACTCCTCAACAACTGAAT; TGTGAGACACATGATTTAAAAGAAATTATATGTAGTTGGAATCCAGGAAGGGTGACAGCG; TTGGTGGGCCCACGTGCTACAAGCTACACTTTAGTTGAAAGTTTTTCAGGAAAATATGTT; AGACTTAAAAGAGCTGAAGCACCTACAAACGAAAGCTATCAATTATTATTTCAAATGCTT; CCAAATCAAGAAATATATAATTTTACTTTGAATGCTCACAATCCGCTGGGTCGATCACAA; TCAACAATTTTAGTTAATATAACTGAAAAAGTTTATCCCCATACTCCTACTTCATTCAAA; GTGAAGGATATTAATTCAACAGCTGTTAAACTTTCTTGGCATTTACCAGGCAACTTTGCA; AAGATTAATTTTTTATGTGAAATTGAAATTAAGAAATCTAATTCAGTACAAGAGCAGCGG; AATGTCACAATCAAAGGAGTAGAAAATTCAAGTTATCTTGTTGCTCTGGACAAGTTAAAT; CCATACACTCTATATACTTTTCGGATTCGTTGTTCTACTGAAACTTTCTGGAAATGGAGC; AAATGGAGCAATAAAAAACAACATTTAACAACAGAAGCCAGTCCTTCAAAGGGGCCTGAT; ACTTGGAGAGAGTGGAGTTCTGATGGAAAAAATTTAATAATCTATTGGAAGCCTTTACCC; ATTAATGAAGCTAATGGAAAAATACTTTCCTACAATGTATCGTGTTCATCAAATGAGGAA; ACACAGTCCCTTTCTGAAATCCCTGATCCTCAGCACAAAGCAGAGATACGACTTGATAAG; AATGACTACATCATCAGCGTAGTGGCTAAAAATTCTGTGGGCTCATCACCACCTTCCAAA; ATAGCGAGTATGGAAATTCCAAATGATGATCTCAAAATAGAACAAGTTGTTGGGATGGGA; AAGGGGATTCTCCTCACCTGGCATTACGACCCCAACATGACTTGCGACTACGTCATTAAG; TGGTGTAACTCGTCTCGGTCGGAACCATGCCTTATGGACTGGAGAAAAGTTCCCTCAAAC; AGCACTGAAACTGTAATAGAATCTGATGAGTTTCGACCAGGTATAAGATATAATTTTTTC; CTGTATGGATGCAGAAATCAAGGATATCAATTATTACGCTCCATGATTGGATATATAGAA; GAATTGGCTCCCATTGTTGCACCAAATTTTACTGTTGAGGATACTTCTGCAGATTCGATA; TTAGTAAAATGGGAAGACATTCCTGTGGAAGAACTTAGAGGCTTTTTAAGAGGATATTTG; TTTTACTTTGGAAAAGGAGAAAGAGACACATCTAAGATGAGGGTTTTAGAATCAGGTCGT; TCTGACATAAAAGTTAAGAATATTACTGACATATCCCAGAAGACACTGAGAATTGCTGAT; CTTCAAGGTAAAACAAGTTACCACCTGGTCTTGCGAGCCTATACAGATGGTGGAGTGGGC; CCGGAGAAGAGTATGTATGTGGTGACAAAGGAAAATTCTGTGGGATTAATTATTGCCATT; CTCATCCCAGTGGCAGTGGCTGTCATTGTTGGAGTGGTGACAAGTATCCTTTGCTATCGG; AAACGAGAATGGATTAAAGAAACCTTCTACCCTGATATTCCAAATCCAGAAAACTGTAAA; GCATTACAGTTTCAAAAGAGTGTCTGTGAGGGAAGCAGTGCTCTTAAAACATTGGAAATG; AATCCTTGTACCCCAAATAATGTTGAGGTTCTGGAAACTCGATCAGCATTTCCTAAAATA; GAAGATACAGAAATAATTTCCCCAGTAGCTGAGCGTCCTGAAGATCGCTCTGATGCAGAG; CCTGAAAACCATGTGGTTGTGTCCTATTGTCCACCCATCATTGAGGAAGAAATACCAAAC; CCAGCCGCAGATGAAGCTGGAGGGACTGCACAGGTTATTTACATTGATGTTCAGTCGATG; TATCAGCCTCAAGCAAAACCAGAAGAAGAACAAGAAAATGACCCTGTAGGAGGGGCAGGC; TATAAGCCACAGATGCACCTCCCCATTAATTCTACTGTGGAAGATATAGCTGCAGAAGAG; GACTTAGATAAAACTGCGGGTTACAGACCTCAGGCCAATGTAAATACATGGAATTTAGTG; TCTCCAGACTCTCCTAGATCCATAGACAGCAACAGTGAGATTGTCTCATTTGGAAGTCCA; TGCTCCATTAATTCCCGACAATTTTTGATTCCTCCTAAAGATGAAGACTCTCCTAAATCT; AATGGAGGAGGGTGGTCCTTTACAAACTTTTTTCAGAACAAACCAAACGATTAA; ; Clone variation with respect to NM_002310.5; 1732 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002310 unedited; NTTGTACGAATTTTGGTATACGACTCACTTATAGGGCGGCCGCGAATCGGCACCANAATA; AGCCCACCCCGCCTCCGAGGCAAGACTAGATGGCTCCAGGATACTCTCGGCGCGCCCGCT; GGCCATCTCGGGCAGGGGAGCCCCACACCCGGCAGGAGGACCAGGACGCGAGCGGCGGCG; CCTCGGCCCTGGGCTGTTTAGGACTGACTGCATTGCACAGATGATGGATATTTACGTATG; TTTGAAACGACCATCCTGGATGGTGGACAATAAAAGAATGAGGACTGCTTCAAATTTCCA; GTGGCTGTTATCAACATTTATTCTTCTATATCTAATGAATCAAGTAAATAGCCAGAAAAA; GGGGGCTCCTCATGATTTGAAGTGTGTAACTAACAATTTGCAAGTGTGGAACTGTTCTTG; GAAAGCACCCTCTGGAACAGGCCGTGGTACTGATTATGAAGTTTGCATTGAAAACAGGTC; CCGTTCTTGTTATCAGTTGGAGAAAACCAGTATTAAAATTCCAGCTCTTTCACATGGTGA; TTATGAAATAACAATAAATTCTCTACATGATTNTGGAAGTTCTACAAGTAAATTCACACT; AAATGAACAAAACGTTTCCTTTATTCCAGATACTCCAGAGATCTTGAATTTGTCTGCTGA; TTTCTCAACCTCTACATTATACCCTAAAGTGGAACGACAGGGGGTTCAGTTTTTCCACAC; CGCTCACATGGTATCTGGGAAAATAAAGTTCTACGTAAAGAGAGAATGGAGCTCGAAAAA; TAGGGACCCCCACACAACTCTGAATGGCAAAGATCACTTATCACTGTAGGTGGGCCCTAG; AATGCCCTTGGAAGGTGCCATCATTTTGTGAAATTAGAGCCTACTTGACATCCTCTTTTT; CTGGCCTCGAAAAGGCAGGACCGGAGCCTGTGAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002310 unedited; AGTTTGGCTCTGTAGGCTATTTTTTNCTTACTGGACATTTCTATGAACTTTATATAGTTT; TAATATACTATGTATGTACAAAATGAATTGCATTTCATTCCAAATTTCCACANATCATAA; AGCTCAGAATTTAGCTGAATCCTAGCAGTTAAAATCAGGATTCATACACAGAGAACCTAA; ATAGATTTTGTCACTTGTCACTCTGGCTTTTTTCCACAACATGGTTTTTAAAAAGATACA; ATATGAGCTTCAAAAAAAATTAATTGATCCATTATTTGCAAATACATTACAGAATCTCAG; ATGACAAAAAACAAACAAACAACCAAAAACAACAAACAAAAAAGACATATCTTATTCGGA; CACTTAAAATTTTCAAGTACTTTTGCCTTAAATATGAAAAGGTAAAATACATAGGCAACC; TGAGGGCAGGGAAAATACAAGATACACTTTTTAGTTTTTCCTTCTGCTTTTCCAAGAACC; ACAATCACTCCAGACATCGCTTATGAGAAACAAGGGTGGGCAGAGGATGCTGTGCTGAAG; GCAATCAATTCTGCACAGCTCACCAAGCAATTGCTGCACCTGCAACACAGGTATGCCCAT; GGATTCAGATCAGTTTATGACCGTTTACTAAGAAAACAGCCCCTTGGGTAACTGGGTCCT; GGTAGAAGCATTCCCTCTTTAGTTCCATATTCAAAAAGGGGCAGGCCTGGCCCCCAATAA; GGGGGCCTGGCGGAAAGACCCCTGGGGTGGGGAGGACCCTCCACCAGAAGGTGGTCAAAG; ATCCCCCCTGCCCCGGTNAGCCCAACCCAATGGGCTGGGGCTCCCTTAGGGGGGAGCGGA; CATCCATTCTGACCTTCTTGGGGCGGCTTCCCCCTGTCTTACCATTGGTTCAACCAACCC; TCCTCGCCTACGCGGGCGCCCGGGACTCCCCGCGTTGATGAACCACCTCCGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002310
• Insert Size: 5550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002310.3, NP_002301.1
• RefSeq Size: 10099 bp
• RefSeq ORF: 3294 bp
• Locus ID: 3977
• UniProt ID: P42702
• Cytogenetics: 5p13.1
• Domains: FN3
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• Summary: This gene encodes a protein that belongs to the type I cytokine receptor family. This protein combines with a high-affinity converter subunit, gp130, to form a receptor complex that mediates the action of the leukemia inhibitory factor, a polyfunctional cytokine that is involved in cellular differentiation, proliferation and survival in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, t(5;8)(p13;q12) with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland. Multiple splice variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2018]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 3. Variants 1, 2, and 3 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.