LY6H (NM_002347) Human Untagged Clone

SKU SC118689

LY6H (untagged)-Human lymphocyte antigen 6 complex, locus H (LY6H), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: LY6H
• Synonyms: NMLY6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002347 edited; ATGCTGCCTGCAGCCATGAAGGGCCTCGGCCTGGCGCTGCTGGCCGTCCTGCTGTGCTCG; GCGCCCGCTCATGGCCTGTGGTGCCAGGACTGCACCCTGACCACCAACTCCAGCCATTGC; ACCCCAAAGCAGTGCCAGCCGTCCGACACGGTGTGTGCCAGTGTCCGAATCACCGATCCC; AGCAGCAGCAGGAAGGATCACTCGGTGAACAAGATGTGTGCCTCCTCCTGTGACTTCGTT; AAGCGACACTTTTTCTCAGACTATCTGATGGGGTTTATTAACTCTGGGATCTTAAAGGTC; GACGTGGACTGCTGCGAGAAGGATTTGTGCAATGGGGCGGCAGGGGCAGGGCACAGCCCC; TGGGCCCTGGCCGGGGGGCTCCTGCTCAGCCTGGGGCCTGCCCTCCTCTGGGCTGGGCCC; TGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002347 unedited; GGTTTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGCCCAGGGTCTGCGAA; CCCGGACCTGCGCGTGGCGCCGCGCCGGGTACCTCCCCCTCTCTGCGTCTTTGTGCATTT; TGCTTCCTTTCGCAGTAAACGTTCTTCCGAGAAATTCCAAGGGGATCTCAGCCAAGCAGA; GGGGGAAGGGATCCTGCCCACGCCTGGGGAGACCGAAGGGCACAGCTCCCCGCGCCGCGC; ACGCCGCCCGAGCCCGGAGTGCGGACACCCCCGGGATGCTTGCGCCCCAGAGGACCCGCG; CCCCAAGCCCCCGCGCCGCCCCCAGGCCCACCCGGAGCATGCTGCCTGCAGCCATGAAGG; GCCTCGGCCTGGCGCTGCTGGCCGTCCTGCTGTGCTCGGCGCCCGCTCATGGCCTGTGGT; GCCAGGACTGCACCCTGACCACCAACTCCAGCCATTGCACCCCAAAGCAGTGCCAGCCGT; CCGACACGGTGTGTGCCAGTGTCCGAATCACCGATCCCAGCAGCAGCAGGAAGGATCACT; CGGTGAACAAGATGTGTGCCTCCTCCTGTGACTTCGTTAAGCGACACTTTTTCTCAGACT; ATCTGATGGGGTTTATTAACTCTGGGATCTTAAAGGTCGACGTGGACTGCTGCGAGAAGG; ATTTGTGCAATGGGGCGGCAGGGGCAGGGCACAGCCCCTGGGCCCTGGCCGGNGGGCTCC; TGCTCAGCCTGGGGCCTGCCCTCCTCTGGGCTGGGCCCTGATGTCTCCTCCTTTCCACGG; NGCTTCTGAGCTTGCTCCCCTGAGCCTGTGGCTGCCCTCTCCCCACCTGGCGTGGCTGGN; GCTGNNGGCAGCCTTGGNCCAGCTCCGTGGCTGTGGCCTGTGGCTCTCACTCTCCCCCGA; CGTGAGCCTNCCTGTCTCTNCNCAGCTCTGAGTCCCAGCAGCTGGACTCTNCAGAAACCA; GCCTCTGGCAGAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002347 unedited; ATCTCTGAACCCGCGGCCGCATNCTAGNGATCGAGNTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTCCCAAGGAACTAAAAAGGGATTTTATTTTTCCG; AAAAAAACCCCTTTCCAGGGGGGGTGGGGTTTAATTTCCCCTCCGGAACCGGGGGGGCCC; CCCCAACCTTAATCCCAAGGCCTCTTGCCAAAAGGGCCTGGTTTCCTGAAAATGCCCACC; TGCCTGGAACTAAAAGCTGGGGGAAAAACAGGGAGGTTTCACGTGGGGGGAGGAGGGAAA; GCCACAGGCCAAAGCCACGGAGCTGGGCCAAGGTTGCCCCAACCCCAAGCCACCCAAGGT; TGGGGAAAGGGCACCCACAGGCTAAGGGGAGCAAGCTAAAAACCCCCGGGGGAAGGAGGA; AACATCAGGGCCCACCCCAAAGGAGGGCAGGCCCCAGGTTGAGAAGGACCCCCCCGGCCA; GGGCCCAGGGGCTGTGCCCTGCCCCTGCCGCCCCATTGAACAAATCTTTCTCGAAGAAGT; CCACGTGAACCTTTAAGATCCCAAAGTTAATAAACCCCATAAAATAGTCGGAAAAAAAGG; GGCCTTAAACGAAGTCACAGGAGGAGGCCCACATTTTGTTCACCGAGGGATCCTTCCTGC; TGCTGCGGGAAACGGAGAATTGGAAACTGGCACAACCGGGTGGGACGGGTGGCACTGCTT; TGGGGGGCAATGCTTGAAATGGGGGGAAAGGGGCAACCCCTGGACAACAAAGCCATTAAC; GGGCGCCAACCACAAAAGAAGGCCAAAAACGCCCAGGCCAAGGCCCTTTATGGCTGGAGG; AAACATTCTTCCGGTGGGGCCTGGGGGCGGTCCGGGGGGCTCTGTGCCCCCGTTCTCTTT; GAGGCAAAACCCCCGGGGGGGTCCCACCTCGGCTTCGGACGGGGCCCGCGCGCGATAAAT; TCGCCTTTGATCTCACAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002347
• Insert Size: 1150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002347.2, NP_002338.1
• RefSeq Size: 957 bp
• RefSeq ORF: 423 bp
• Locus ID: 4062
• UniProt ID: O94772
• Cytogenetics: 8q24.3
• Domains: LU
• Summary: Believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro inhibits alpha-3:beta-4-containing nAChRs maximum response. May play a role in the intracellular trafficking of alpha-7-containing nAChRs and may inhibit their expression at the cell surface. Seems to inhibit alpha-7/CHRNA7 signaling in hippocampal neurons.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the shortest transcript and encodes the shorter isoform (a). CCDS Note: This CCDS represents the shorter LY6H isoform. NM_002347.4 is based on an alternative 5' end, as found in the ESTs DB471677.1, BP347868.1 and EB385547.1. Other alternative 5' ends exist, as in AK314139.1, BC028894.1, AB012293.1 and BC030192.1, and these contain an upstream in-frame translation start codon which results in an isoform that is 21 aa longer at the N-terminus. The upstream start codon has a weaker Kozak signal than the downstream start codon, so these variants may also produce the shorter isoform, as suggested in PMID:9799603.