Lumican (LUM) (NM_002345) Human Untagged Clone

SKU SC118686

LUM (untagged)-Human lumican (LUM)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Lumican
• Synonyms: LDC; SLRR2D
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118686 sequence for NM_002345 edited (data generated by NextGen Sequencing); ATGAGTCTAAGTGCATTTACTCTCTTCCTGGCATTGATTGGTGGTACCAGTGGCCAGTAC; TATGATTATGATTTTCCCCTATCAATTTATGGGCAATCATCACCAAACTGTGCACCAGAA; TGTAACTGCCCTGAAAGCTACCCAAGTGCCATGTACTGTGATGAGCTGAAATTGAAAAGT; GTACCAATGGTGCCTCCTGGAATCAAGTATCTTTACCTTAGGAATAACCAGATTGACCAT; ATTGATGAAAAGGCCTTTGAGAATGTAACTGATCTGCAGTGGCTCATTCTAGATCACAAC; CTTCTAGAAAACTCCAAGATAAAAGGGAGAGTTTTCTCTAAATTGAAACAACTGAAGAAG; CTGCATATAAACCACAACAACCTGACAGAGTCTGTGGGCCCACTTCCCAAATCTCTGGAG; GATCTGCAGCTTACTCATAACAAGATCACAAAGCTGGGCTCTTTTGAAGGATTGGTAAAC; CTGACCTTCATCCATCTCCAGCACAATCGGCTGAAAGAGGATGCTGTTTCAGCTGCTTTT; AAAGGTCTTAAATCACTCGAATACCTTGACTTGAGCTTCAATCAGATAGCCAGACTGCCT; TCTGGTCTCCCTGTCTCTCTTCTAACTCTCTACTTAGACAACAATAAGATCAGCAACATC; CCTGATGAGTATTTCAAGCGTTTTAATGCATTGCAGTATCTGCGTTTATCTCACAACGAA; CTGGCTGATAGTGGAATACCTGGAAATTCTTTCAATGTGTCATCCCTGGTTGAGCTGGAT; CTGTCCTATAACAAGCTTAAAAACATACCAACTGTCAATGAAAACCTTGAAAACTATTAC; CTGGAGGTCAATCAACTTGAGAAGTTTGACATAAAGAGCTTCTGCAAGATCCTGGGGCCA; TTATCCTACTCCAAGATCAAGCATTTGCGTTTGGATGGCAATCGCATCTCAGAAACCAGT; CTTCCACCGGATATGTATGAATGTCTACGTGTTGCTAACGAAGTCACTCTTAATTAA; ; Clone variation with respect to NM_002345.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002345 unedited; GTATTTGTAAACGACTACTATAGGCGGCCGCGCAATTCGCACGAGGGTTCACAGCATATA; TTGGTGGATTCTTGTCCATAGTGCATCTGCTTTAAGAATTAACGAAAGCAGTGTCAAGAC; AGTAAGGATTCAAACCATTTGCCAAAAATGAGTCTAAGTGCATTTACTCTCTTCCTGGCA; TTGATTGGTGGTACCAGTGGCCAGTACTATGATTATGATTTTCCCCTATCAATTTATGGG; CAATCATCACCAAACTGTGCACCAGAATGTAACTGCCCTGAAAGCTACCCAAGTGCCATG; TACTGTGATGAGCTGAAATTGAAAAGTGTACCAATGGTGCCTCCTGGAATCAAGTATCTT; TACCTTAGGAATAACCAGATTGACCATATTGATGAAAAGGCCTTTGAGAATGTAACTGAT; CTGCAGTGGCTCATTCTAGATCACAACCTTCTAGAAAACTCCAAGATAAAAGGGAGAGTT; TTCTCTAAATTGAAACAACTGAAGAAGCTGCATATAAACCACAACAACCTGACAGAGTCT; GTGGGCCCACTTCCCAAATCTCTGGAGGATCTGCAGCTTACTCATAACAAGATCACAAAG; CCTGNGCTCTTTCTGAAGGATTGGTAAACCTGACCTTCATCCATCTCCAGCACAATCCGC; TGAAAGAGGATGCTGTTTCACCTGCTCTTAAAAGTCTTAAATCACTCGAATACCTTGACT; TGAGCCTCAATCAGATAGCCAGACTGCCTTTTGGTCTCCCTGCCTCTTTTTTAACTCTCT; ACTTAGACCACAATAAGATCAGCACATCCCTGAAGAGTATTTCAAGCGTTTTAATGCATT; GCAGGATTCTGCGTTATCTTACAACCAACTGGCTGATAATGGAATACCTGGAAATCTTTC; AAGGGCCTCCCTGCTGAACTGAACTGTCCTAAACAAGCTATAAACT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002345 unedited; ACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTGCCCTTTGCTATGGTTTATTTTGCTAGTAGCTTATTAAACATAACATGCCAATA; ATCAAAGAGAAACCTACCTGACTTAAAGTGAAAAATAATTCTAAAAAAGTTTCACTAGGG; AAGTATGCAAATTCTTATTCTAAAAATACTTCTTTAAGTGCCTGAAGCTTCATGTATTTT; GCAATATTCTTGGCCTCAATATCTACCACCTATTTTTTAACCCGACCAATTTGAATGGAT; CAAGATAATTTGGGGCCAGAAAGTAACATCCCTATGTATTTAATCCAAGCTTTGAGGAAC; CTTAAGATTTAAGGGTTATAAAACTTGGCTGGTTTCCATGCCACCAGTAAAAGGGTTTCG; CCATCATTTGCCCGTAGAAATAAAAAAACACTAAATTTACCAATAAAGCACTTGAGTTCG; AAGGCCATTCCCGGATATGTGGGCCGTCCTGGGACGAAAAAAGCCCGCCCTTCATCTTTT; CTTTAAAAAAAAAAAAAGGTTACAAAACATTTCCCTTAAATTTTAAAATCCATGGGAAGT; AATCAACCCGCCATAACAATTGGGTCCCATGAAAACCGCCCCACCGAAAACCCTCCCCCC; TACATTTTGGTCCCAGCACCCCATTTTTAATAAGAAGGGCCTCCCTCTGCACCACCCTAA; CATTTCTTCTTATCCGGGGGAAAACCGGTTTTGCAAACCATCGCCTTCCCAACCAATGCT; TTGTCTGGGGGAGGATAAGGCCCCGCGATCTTGCAAAACCTCTTTTGGACAACTTCCTAA; CTGATGACCCTCCAGCAAACTTCCACGGGCTCCTTGCACCCTGGCCACCTACAACCTCCC; CTN;
• Restriction Sites: NotI-NotI
• ACCN: NM_002345
• Insert Size: 1970 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002345.3, NP_002336.1
• RefSeq Size: 2116 bp
• RefSeq ORF: 1017 bp
• Locus ID: 4060
• UniProt ID: P51884
• Cytogenetics: 12q21.33
• Domains: LRR, LRRNT, LRR_BAC, LRR_SD22, LRR_TYP
• Protein Families: Secreted Protein
• Summary: This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]