Manic Fringe (MFNG) (NM_002405) Human Untagged Clone

SKU SC118671

MFNG (untagged)-Human MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (MFNG), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Manic Fringe
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002405 edited; GAATTCGGCACGAGGGCCAACCAATGCAGTGCCGGCTCCCGCGGGGCCTGGCTGGAGCCC; TCCTCACCCTCCTGTGCATGGGGCTCCTGTGTCTGCGGTACCACTTGAACCTGTCCCCGC; AGCGGGTACAAGGGACCCCCGAGCTGAGCCAGCCGAACCCGGGGCCCCCTAAGCTACAGC; TACACGATGTCTTCATTGCAGTGAAGACGACCCGGGCTTTCCACCGCTTGCGCCTGGAGC; TGCTGCTTGACACGTGGGTTTCCAGGACCAGGGAACAGACATTTGTCTTCACCGACAGCC; CAGACAAAGGCCTCCAGGAGAGACTGGGGTCCCACCTTGTGGTCACCAACTGCTCCGCGG; AACACAGCCACCCAGCTCTGTCCTGCAAGATGGCTGCTGAGTTCGACACCTTCTTGGCCA; GTGGGCTTAGGTGGTTCTGCCATGTGGACGATGACAACTATGTGAACCCAAGGGCGCTGC; TGCAGCTTCTGAGAGCCTTCCCGCTGGCCCGCGACGTCTATGTGGGAAGGCCCAGCCTGA; ACCGGCCCATCCATGCCTCAGAGCCACAGCCCCACAACCGCACGAGGCTGGTACAGTTCT; GGTTTGCCACTGGGGGTGCTGGCTTCTGCATCAATCGCAAACTGGCTTTGAAGATGGCTC; CGTGGGCCAGTGGCTCCCGTTTCATGGACACATCTGCTCTCATCCGGCTGCCTGATGACT; GCACCATGGGCTATATCATTGAGTGCAAGCTGGGCGGCCGCCTGCAGCCCAGCCCCCTCT; TTCACTCCCACCTGGAGACCCTGCAGCTGCTGAGGACTGCACAGCTCCCAGAACAGGTCA; CCCTCAGCTACGGTGTCTTTGAGGGGAAACTCAACGTCATTAAGCTACAGGGCCCCTTCT; CCCCGGAGGAGGACCCCTCCAGATTTCGCTCCCTCCATTGTCTGCTCTATCCAGATACAC; CCTGGTGTCCCCAGCTGGGTGCCCGATGAATCCTGAACTGCTGGGCAAAGGTTGGGCAGA; GACTTCTGGGTGTGCCTTGGCTCCCAAGGTGGCACTGTGGGTCCCTGGCAAGTGTCTTGT; GATAGGCAGTCCCTGGCAGGGCCTTCGGGTGGTTGGCAAGCCCAGGATCTGAGTGGCAAT; TGGCACTGAAGGCACCCCAGGCCCCTGGGAGGTGAGTTAGACAGCCCAGGGGACCAGGTG; GACCAGGTGGTGGCCAGAGAGGCTCCAGGGGCTAGACTCCCTCAGGAGGCTGAATTGAAA; AAGGGCAGGGGGCACTTGAGCTGGGCTGGGGCTCAGGGGTCCTAACCCTTTAGGCAGTGA; CATGGCCTCTGGGTGGGGTCTGGCCGTTGGCCCTGGCTAATGTCTCTCAGTCATTCCCCT; GGGGCTCAAGCGCTGGGCCGCCCACTCCTGCCTCCCTCATCTGTGTCCCGAGTTCCTGAA; GGGACATGGGTGGAATGATGGCAGAATCCAGGGTCTGCAGCACCTGCTGTTGTTGCCAAC; CAGTCTCCCAAAGCTCCTTGCTCCCCACCCCTTGCGAACAGGACCAGATTTTGTTTGGAG; CCTCAGCATGCCGGGGCCCAGATGATGGAGCATAACGGGTCCCAGCCAATTGTGATGATC; CTTTTTGCTCATTTCCCAGCCTTTCTTGCTGTTAGGGGCTACCATGGGACCAGCTCTGGC; CAGAGGGAACTAAGCAAATCCAATAGAGATGTTTCTGGGGAAGGTTTTGCAGCCCACTCC; CCATCTTCCTGCTATAAATGTGGGTGTGATGGCTGGATCTGGGGCAGCCACCTTGCTACC; ATGAAGGAAAGGCCAAGACAATCATCCACAGCTATTCCCTCCAGCATCTGGTTCTGTACA; AAAATTAAATGCTTATTTGTTTAAGTCAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002405 unedited; TGTGCAAATTTTGTNATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCCAA; CCAATGCAGTGCCGGCTCCCGCGGGGCCTGGCTGGAGCCCTCCTCACCCTCCTGTGCATG; GGGCTCCTGTGTCTGCGGTACCACTTGAACCTGTCCCCGCAGCGGGTACAAGGGACCCCC; GAGCTGAGCCAGCCGAACCCGGGGCCCCCTAAGCTACAGCTACACGATGTCTTCATTGCA; GTGAAGACGACCCGGGCTTTCCACCGCTTGCGCCTGGAGCTGCTGCTTGACACGTGGGTT; TCCAGGACCAGGGAACAGACATTTGTCTTCACCGACAGCCCAGACAAAGGCCTCCAGGAG; AGACTGGGGTCCCACCTTGTGGTCACCAACTGCTCCGCGGAACACAGCCACCCAGCTCTG; TCCTGCAAGATGGCTGCTGAGTTCGACACCTTCTTGGCCAGTGGGCTTAGGTGGTTCTGC; CATGTGGACGATGACAACTATGTGAACCCAAGGGCGCTGCTGCAGCTTCTGAGAGCCTTC; CCGCTGGCCCGCGACGTCTATGTGGGAAGGCCCAGCCTGAACCGGCCCATCCATGCCTCA; GAGCCACAGCCCCACAACCGCACGAGGCTGGTACAGTTCTGGTTTGCCACTGGGGGTGCT; GGCTTCTGCATCAATCGCAAACTGGCTTTGAAGATGGCTCCGTGGGCCAGTGGCTCCCGT; TTCATGGACACATCTGCTCTCATCCGGCTGCCTGATGACTGCACCATGGGCTATATCATT; GAGTGCAAGCTGGGCGGCCGCCTGCAGCCCAGNCCCCTCTTTCACTCCCACCCTGGAGAC; CCTGCAGCTGCTGAAGACTGCACAGCTCCCAGAACAGGTCACCCTCAGCTACGGTGTCCT; TTGAGGGGAACCTCCACC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002405 unedited; TGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGACTTAAACAAAT; AAGCATTTAATTTTTGTACAGAACCAGATGCTGGAGGGAATAGCTGTGGATGATTGTCTT; GGCCTTTCCTTCATGGTAGCAAGGTGGCTGCCCCAGATCCAGCCATCACACCCACATTTA; TAGCAGGAAGATGGGGAGTGGGCTGCAAAACCTTCCCCAGAAACATCTCTATTGGATTTG; CTTAGTTCCCTCTGGCCAGAGCTGGTCCCATGGTAGCCCCTAACAGCAAGAAAGGCTGGG; AAATGAGCAAAAAGGATCATCACAATTGGCTGGGACCCGTTATGCTCCATCATCTGGGCC; CCGGCATGCTGAGGCTCCAAACAAAATCTGGTCCTGTTCGCAAGGGGTGGGGAGCAAGGA; GCTTTGGGAGACTGGTTGGCAACAACAGCAGGTGCTGCAGACCCTGGATTCTGCCATCAT; TCCACCCATGTCCCTTCAGGAACTNCGGACACANATGANGGAGGCAGGAGTGGGCGGCCC; ANCGCTTGAGCCCCCAGGGAATGACTGAGAGACATTAGCCAGGGCCAACGGCCAGACCCC; ACCCCAGAGCCATGTCACTGCCTAAAGGGTAGGACCCCTGAGCCCCAGCCCAGCTCAAGT; GCCCCTGCCCTTTTTCAATCAGCCTCCCTGAGGAGTCTAGCCCCTGGAGCTCTCTGGCCA; CCACCTGGTCCACCTGTCCCCTGGGCTGNCTACTTACCTCCCAGGGGCCTGGGTTGCCTC; AGTGCCATTGCCACTCAAACCTGGGCTTGCCACCACCCAAAGCCCTGGCAGNACTGNCTA; TACAGNAACCTGNCAGGGACCCAAAGGCACCTGGGNAGCCAGGCCACCCAAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_002405
• Insert Size: 1820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002405.2, NP_002396.2
• RefSeq Size: 2040 bp
• RefSeq ORF: 966 bp
• Locus ID: 4242
• UniProt ID: O00587
• Cytogenetics: 22q13.1
• Domains: Fringe
• Protein Families: Druggable Genome
• Protein Pathways: Notch signaling pathway
• Summary: This gene is a member of the glycosyltransferase 31 gene family. Members of this gene family, which also includes the LFNG (GeneID: 3955) and RFNG (GeneID: 5986) genes, encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, these proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. The protein encoded by this gene may control Notch signaling in claudin-low breast cancer. [provided by RefSeq, May 2018]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).