CD99 (NM_002414) Human Untagged Clone

SKU SC118640

CD99 (untagged)-Human CD99 molecule (CD99), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CD99
• Synonyms: HBA71; MIC2; MIC2X; MIC2Y; MSK5X
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC118640 representing NM_002414.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; ATGGCCCGCGGGGCTGCGCTGGCGCTGCTGCTCTTCGGCCTGCTGGGTGTTCTGGTCGCCGCCCCGGAT; GGTGGTTTCGATTTATCCGATGCCCTTCCTGACAATGAAAACAAGAAACCCACTGCAATCCCCAAGAAA; CCCAGTGCTGGGGATGACTTTGACTTAGGAGATGCTGTTGTTGATGGAGAAAATGACGACCCACGACCA; CCGAACCCACCCAAACCGATGCCAAATCCAAACCCCAACCACCCTAGTTCCTCCGGTAGCTTTTCAGAT; GCTGACCTTGCGGATGGCGTTTCAGGTGGAGAAGGAAAAGGAGGCAGTGATGGTGGAGGCAGCCACAGG; AAAGAAGGGGAAGAGGCCGACGCCCCAGGCGTGATCCCCGGGATTGTGGGGGCTGTCGTGGTCGCCGTG; GCTGGAGCCATCTCTAGCTTCATTGCTTACCAGAAAAAGAAGCTATGCTTCAAAGAAAATGAACAAGGG; GAGGTGGACATGGAGAGCCACCGGAATGCCAACGCAGAGCCAGCTGTTCAGCGTACTCTTTTAGAGAAA; TAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002414 unedited; AAGTTCAGATTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGCGTCCCTG; CGCGCTCTGGGCGCACCATGGCCCGCGGGGCTGCGCTGGCGCTGCTGCTCTTCGGCCTGC; TGGGTGTTCTGGTCGCCGCCCCGGATGGTGGTTTCGATTTATCCGATGCCCTTCCTGACA; ATGAAAACAAGAAACCCACTGCAATCCCCAAGAAACCCAGTGCTGGGGATGACTTTGACT; TAGGAGATGCTGTTGTTGATGGAGAAAATGACGACCCACGACCACCGAACCCACCCAAAC; CGATGCCAAATCCAAACCCCAACCACCCTAGTTCCTCCGGTAGCTTTTCAGATGCTGACC; TTGCGGATGGCGTTTCAGGTGGAGAAGGAAAAGGAGGCAGTGATGGTGGAGGCAGCCACA; GGAAAGAAGGGGAAGAGGCCGACGCCCCAGGCGTGATCCCCGGGATTGTGGGGGCTGTCG; TGGTCGCCGTGGCTGGAGCCATCTCTAGCTTCATTGCTTACCAGAAAAAGAAGCTATGCT; TCAAAGAAAATGAACAAGGGGAGGTGGACATGGAGAGCCACCGGAATGCCAACGCAGAGC; CAGCTGTTCAGCGTACTCTTTTAGAGAAATAGAAGATTGTCGGCAGAAACAGCCCAGGCG; TTGGCAGCAGGGTTAGAACAGCTGCCTGAGGCTCCTCCCTGAANGACACCTGCCTGAGAG; CAGAGATGGAGGCCTTCTGTTCACGGCGGATTCTTTGTTTTAATCTTGCGATGTGCTTTG; CTTGTTGCTGGGGCGATGATGTTTACTAACGATGAATTTTACATCCAAAGGGGGATAGGC; ACTTGNACCCCCATTCTCCAAGCCCGGGGGGGCGGTTTNCCCATGGATGTGAAAGGGCTG; CCCATTATAAGTCCNTGTNACTCANATGTCAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002414 unedited; CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTCTAATTTCTTTTTATT; AGTCTGATATGAAAAACAAATATCCCTACAAATGAAAGAAATATTGAAGAAACAAATTGA; AGGGCTTACCTTATTTTAATTAAAAAGAAGTCGGGGCTACCAGGGGTTTCAGACGTGCTC; GATGGACACGTGATTTGTAAACAGCCAAGATTCTGGGGGACGGGGGGGTGCCTCGGTGGG; GTTGACATTTGAGTTACAGGGACTTAATAATGGCCAGCCTTTCACATCCCATGGGAAACC; GCCCCCCCGGGCCTTGGAGAATGGGGGTCCAAGTGCCTATCCCCCTTTGGATGTAAAATT; CATCGTTAGTAAACATCATCCGCCCAGCAACAAGCAAAGCACATCGCAAGATTAAAACAA; AGAATCCGCCGTGAACAGAAGGCCTCCATCTCTGCTCTCAGGCAGGTGTCCTTCAGGGAG; GAGCCTCAGGCAGCTGTTCTAACCCTGCTGCCAACGCCTGGGCTGTTTCTGCCGACAATC; TTCTATTTCTCTAAAAGAGTACGCTGAACAGCTGGCTCTGCGTTGGCATTCCGGTGGCTC; TCCATGTCCACCTCCCCTTGTTCATTTTCTTTGAAGCATAGCTTCTTTTTCTGGTAAGCA; ATGAAGCTAGAGATGGCTCCAGCCACGGCGACCACGACAGCCCCCACAATCCCGGGGATC; ACGCCTGGGGCGTCGGCCTCTTCCCCTTCTTTCCTGTGGCTGCCTNCACCATCACTGCCT; NCTTTTNCTTCTNCACCTGAAACGCCATCCGCAAGGTCAGCATCTTGAAAGCTACCGGNA; GAACTAGGNTGGGTTGGGGTTNGGNNATTGCATCGNCNTTGGTGGGTTCGGTGGCCNGGG; TTCGTNATTTTCTCATCAACACAGCATCTNCTAGTCAAGTCATCCCAGACTGGGGTTCTT; GGGGATGGCAGGGGGTTCTGTTTCATTTCAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002414
• Insert Size: 555 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002414.3
• RefSeq Size: 1255 bp
• RefSeq ORF: 555 bp
• Locus ID: 4267
• UniProt ID: P14209
• Cytogenetics: X;Y
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration
• MW: 18.8 kDa
• Summary: The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016]; Transcript Variant: This variant (1, also known as type I) encodes the longest isoform (a, also known as CD99wt).