CD99 (NM_002414) Human Untagged Clone

CAT#: SC118640

CD99 (untagged)-Human CD99 molecule (CD99), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD99
• Synonyms: HBA71; MIC2; MIC2X; MIC2Y; MSK5X
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118640 sequence for NM_002414 edited (data generated by NextGen Sequencing)
  ATGGCCCGCGGGGCTGCGCTGGCGCTGCTGCTCTTCGGCCTGCTGGGTGTTCTGGTCGCC
  GCCCCGGATGGTGGTTTCGATTTATCCGATGCCCTTCCTGACAATGAAAACAAGAAACCC
  ACTGCAATCCCCAAGAAACCCAGTGCTGGGGATGACTTTGACTTAGGAGATGCTGTTGTT
  GATGGAGAAAATGACGACCCACGACCACCGAACCCACCCAAACCGATGCCAAATCCAAAC
  CCCAACCACCCTAGTTCCTCCGGTAGCTTTTCAGATGCTGACCTTGCGGATGGCGTTTCA
  GGTGGAGAAGGAAAAGGAGGCAGTGATGGTGGAGGCAGCCACAGGAAAGAAGGGGAAGAG
  GCCGACGCCCCAGGCGTGATCCCCGGGATTGTGGGGGCTGTCGTGGTCGCCGTGGCTGGA
  GCCATCTCTAGCTTCATTGCTTACCAGAAAAAGAAGCTATGCTTCAAAGAAAATNNNGAA
  CAAGGGGAGGTGGACATGGAGAGCCACCGGAATGCCAACGCAGAGCCAGCTGTTCAGCGT
  ACTCTTTTAGAGAAATAG
  
  Clone variation with respect to NM_002414.3
  475 g=>n;476 c=>n;477 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002414 unedited
  AAGTTCAGATTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGCGTCCCTG
  CGCGCTCTGGGCGCACCATGGCCCGCGGGGCTGCGCTGGCGCTGCTGCTCTTCGGCCTGC
  TGGGTGTTCTGGTCGCCGCCCCGGATGGTGGTTTCGATTTATCCGATGCCCTTCCTGACA
  ATGAAAACAAGAAACCCACTGCAATCCCCAAGAAACCCAGTGCTGGGGATGACTTTGACT
  TAGGAGATGCTGTTGTTGATGGAGAAAATGACGACCCACGACCACCGAACCCACCCAAAC
  CGATGCCAAATCCAAACCCCAACCACCCTAGTTCCTCCGGTAGCTTTTCAGATGCTGACC
  TTGCGGATGGCGTTTCAGGTGGAGAAGGAAAAGGAGGCAGTGATGGTGGAGGCAGCCACA
  GGAAAGAAGGGGAAGAGGCCGACGCCCCAGGCGTGATCCCCGGGATTGTGGGGGCTGTCG
  TGGTCGCCGTGGCTGGAGCCATCTCTAGCTTCATTGCTTACCAGAAAAAGAAGCTATGCT
  TCAAAGAAAATGAACAAGGGGAGGTGGACATGGAGAGCCACCGGAATGCCAACGCAGAGC
  CAGCTGTTCAGCGTACTCTTTTAGAGAAATAGAAGATTGTCGGCAGAAACAGCCCAGGCG
  TTGGCAGCAGGGTTAGAACAGCTGCCTGAGGCTCCTCCCTGAANGACACCTGCCTGAGAG
  CAGAGATGGAGGCCTTCTGTTCACGGCGGATTCTTTGTTTTAATCTTGCGATGTGCTTTG
  CTTGTTGCTGGGGCGATGATGTTTACTAACGATGAATTTTACATCCAAAGGGGGATAGGC
  ACTTGNACCCCCATTCTCCAAGCCCGGGGGGGCGGTTTNCCCATGGATGTGAAAGGGCTG
  CCCATTATAAGTCCNTGTNACTCANATGTCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002414 unedited
  CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTCTAATTTCTTTTTATT
  AGTCTGATATGAAAAACAAATATCCCTACAAATGAAAGAAATATTGAAGAAACAAATTGA
  AGGGCTTACCTTATTTTAATTAAAAAGAAGTCGGGGCTACCAGGGGTTTCAGACGTGCTC
  GATGGACACGTGATTTGTAAACAGCCAAGATTCTGGGGGACGGGGGGGTGCCTCGGTGGG
  GTTGACATTTGAGTTACAGGGACTTAATAATGGCCAGCCTTTCACATCCCATGGGAAACC
  GCCCCCCCGGGCCTTGGAGAATGGGGGTCCAAGTGCCTATCCCCCTTTGGATGTAAAATT
  CATCGTTAGTAAACATCATCCGCCCAGCAACAAGCAAAGCACATCGCAAGATTAAAACAA
  AGAATCCGCCGTGAACAGAAGGCCTCCATCTCTGCTCTCAGGCAGGTGTCCTTCAGGGAG
  GAGCCTCAGGCAGCTGTTCTAACCCTGCTGCCAACGCCTGGGCTGTTTCTGCCGACAATC
  TTCTATTTCTCTAAAAGAGTACGCTGAACAGCTGGCTCTGCGTTGGCATTCCGGTGGCTC
  TCCATGTCCACCTCCCCTTGTTCATTTTCTTTGAAGCATAGCTTCTTTTTCTGGTAAGCA
  ATGAAGCTAGAGATGGCTCCAGCCACGGCGACCACGACAGCCCCCACAATCCCGGGGATC
  ACGCCTGGGGCGTCGGCCTCTTCCCCTTCTTTCCTGTGGCTGCCTNCACCATCACTGCCT
  NCTTTTNCTTCTNCACCTGAAACGCCATCCGCAAGGTCAGCATCTTGAAAGCTACCGGNA
  GAACTAGGNTGGGTTGGGGTTNGGNNATTGCATCGNCNTTGGTGGGTTCGGTGGCCNGGG
  TTCGTNATTTTCTCATCAACACAGCATCTNCTAGTCAAGTCATCCCAGACTGGGGTTCTT
  GGGGATGGCAGGGGGTTCTGTTTCATTTCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002414
• Insert Size: 1140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002414.3, NP_002405.1
• RefSeq Size: 1255 bp
• RefSeq ORF: 558 bp
• Locus ID: 4267
• UniProt ID: P14209
• Cytogenetics: X;Y
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration
• Gene Summary: The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016]
  Transcript Variant: This variant (1, also known as type I) encodes the longest isoform (a, also known as CD99wt).