MTAP (NM_002451) Human Untagged Clone

SKU SC118635

MTAP (untagged)-Human methylthioadenosine phosphorylase (MTAP)

Product Data

• Type: Human Untagged Clone
• Target Symbol: MTAP
• Synonyms: BDMF; c86fus; DMSFH; DMSMFH; HEL-249; LGMBF; MSAP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118635 sequence for NM_002451 edited (data generated by NextGen Sequencing); ATGGCCTCTGGCACCACCACCACCGCCGTGAAGATTGGAATAATTGGTGGAACAGGCCTG; GATGATCCAGAAATTTTAGAAGGAAGAACTGAAAAATATGTGGATACTCCATTTGGCAAG; CCATCTGATGCCTTAATTTTGGGGAAGATAAAAAATGTTGATTGCATCCTCCTTGCAAGG; CATGGAAGGCAGCACACCATCATGCCTTCAAAGGTCAACTACCAGGCGAACATCTGGGCT; TTGAAGGAAGAGGGCTGTACACATGTCATAGTGACCACAGCTTGTGGCTCCTTGAGGGAG; GAGATTCAGCCCGGCGATATTGTCATTATTGATCAGTTCATTGACAGGACCACTATGAGA; CCTCAGTCCTTCTATGATGGAAGTCATTCTTGTGCCAGAGGAGTGTGCCATATTCCAATG; GCTGAGCCGTTTTGCCCCAAAACGAGAGAGGTTCTTATAGAGACTGCTAAGAAGCTAGGA; CTCCGGTGCCACTCAAAGGGGACAATGGTCACAATCGAGGGACCTCGTTTTAGCTCCCGG; GCAGAAAGCTTCATGTTCCGCACCTGGGGGGCGGATGTTATCAACATGACCACAGTTCCA; GAGGTGGTTCTTGCTAAGGAGGCTGGAATTTGTTACGCAAGTATCGCCATGGCGACAGAT; TATGACTGCTGGAAGGAGCACGAGGAAGCAGTTTCGGTGGACCGGGTCTTAAAGACCCTG; AAAGAAAACGCTAATAAAGCCAAAAGCTTACTGCTCACTACCATACCTCAGATAGGGTCC; ACAGAATGGTCAGAAACCCTCCATAACCTGAAGAATATGGCCCAGTTTTCTGTTTTATTA; CCAAGACATTAA; ; Clone variation with respect to NM_002451.3; 166 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002451 unedited; ATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGCCACCGCTCTGTGGC; TCGCTTGGTTCCCTTAGTCCCGAGCGCTCGCCCACTGCAGATTCCTTTCCCGTGCAGACA; TGGCCTCTGGCACCACCACCACCGCCGTGAAGATTGGAATAATTGGTGGAACAGGCCTGG; ATGATCCAGAAATTTTAGAAGGAAGAACTGAAAAATATGTGGATACTCCATTTGGCAAGC; CATCTGATGCCTTAATTTTGGGGAAGATAAAAAATGTTGATTGCATCCTCCTTGCAAGGC; ATGGAAGGCAGCACACCATCATGCCTTCAAAGGTCAACTACCAGGCGAACATCTGGGCTT; TGAAGGAAGAGGGCTGTACACATGTCATAGTGACCACAGCTTGTGGCTCCTTGAGGGAGG; AGATTCAGCCCGGCGATATTGTCATTATTGATCAGTTCATTGACAGGACCACTATGAGAC; CTCAGTCCTTCTATGATGGAAGTCATTCTTGTGCCAGAGGAGTGTGCCATATTCCAATGG; CTGAGCCGTTTTGCCCCAAAACGAGAGAGGTTCTTATAGAGACTGCTAAGAAGCTAGGAC; TCCGGTGCCACTCAAAGGGGACAATGGTCACAATCGAGGGACCTCGTTTTAGCTCCCGGG; CAGAAAGCTTCATGTTCCGCACCTGGGGGGCGGATGTTATCAACATGACCACAGTTCCAG; AAGTGGTTCTTGCTAAGGACGCTGTAATTTGTTACGCAAGTATCGCCATGCCGACAGATT; ATGACTGCTGGCAAGAGCACCATGAAGCAGTTTCCGTGCACCCGGTCCTTAAGACCTTGA; AGCAAACGCTATAAAGCCAAAAGCTACTGCTCATACCATACCTCAGATAGGTCCCAGAAT; GGTCANAACCCTCCATACCTGGAGATATGCCCAGCTTTTGTCTTATACCAGCAATAAATG; ACATGCTGCCCAGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002451 unedited; CGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTCTAAATCCACTTTTATTTTTACAAC; AACTTTACTATTTGATTATATTCACTGTAGAAAAATGTGAAGACACAGAAAGATTCAAAG; AAAATAAAAATTATCCCAGTATCCCTCCCCCAAATACAATAACAAGGTTATATATATTTC; TTTCTAGTTTTTTTGTATATTTACATAGCATTTTAAAGCAAAACTAGTACTATAAAATGC; TACCATATCCTTTTTTCACTTCATATATAGAGCAACCGTGTCTTTTTAGGACATTGAAAT; ATGCAAAAAGGATTTTCAGTTGGCTGATTCACAATCTAATTATAACTAAGACATAATTTA; CCTCCAATTGCAAGGGATTCAGATTGTTTCAATTATTTATTATTATAACTATCATTATTA; AACACCCTTCTTTATATATAATTCTTTGTATGCATCTCTGATATGGTTTTAAAGAGAAAT; TGCTAGATGTAGAGTTCCCAGCACAAAGTACGTCCGTATTTTTAAGTCTTTAGATGCATT; TTACCAAATTGCCCTCCAGAAATACTGTACCAATTTACAGTCGCATCAATAAATAACCAA; AGAGTCCCATTTCTTCCTCACATTCTTTGGCAGTATAGGAAAGTAAAGATTACCCTCCAC; CCTTTATTGTTGCAAATTTATAGGGGGAGAAGAGATTGGCGGGGTTTTTTTTCCCCTTAA; ATGAATATTTTTTACCCATGTTACCAGCCATTGGCTTTCTTCTGATTTTGAAGTGGCCAA; TCATTCAAGAATCTTTATACACCAATGCATGTTCTTTTACAAAATATCTTTGTAGCCAGG; GCTTGAAGCTGATGTTTTTCCATATTTTTCAGTTAACAGAATTCCAATGATGGATTAATG; CTCTTTTTTCCGGACACCAGCTTTTATTGCTTGCTAAAAAAAAAGTGGCCTTTTTCAGTT; TGAAGTTCTGACTACCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002451
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002451.3, NP_002442.2
• RefSeq Size: 4937 bp
• RefSeq ORF: 852 bp
• Locus ID: 4507
• UniProt ID: Q13126
• Cytogenetics: 9p21.3
• Domains: Mtap_PNP
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• Summary: This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008]