Myosin (MYL4) (NM_002476) Human Untagged Clone

SKU SC118608

MYL4 (untagged)-Human myosin, light chain 4, alkali, atrial, embryonic (MYL4), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Myosin
• Synonyms: ALC1; AMLC; GT1; PRO1957
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118608 sequence for NM_002476 edited (data generated by NextGen Sequencing); ATGGCTCCCAAGAAGCCTGAGCCTAAGAAGGAGGCAGCCAAGCCAGCTCCAGCTCCAGCT; CCAGCCCCTGCACCAGCCCCTGCCCCAGCTCCTGAGGCTCCCAAGGAACCTGCCTTTGAC; CCCAAGAGTGTAAAGATAGACTTCACTGCCGACCAGATTGAAGAGTTCAAAGAGGCCTTT; TCATTGTTTGACCGGACCCCGACTGGAGAGATGAAGATCACCTACGGCCAGTGCGGGGAT; GTACTGCGGGCCCTGGGCCAGAACCCTACCAATGCCGAGGTGCTGCGTGTGCTGGGCAAG; CCCAAGCCTGAAGAGATGAATGTCAAGATGCTGGACTTTGAGACGTTCTTGCCCATCCTG; CAGCACATTTCCCGCAACAAGGAGCAGGGCACCTATGAGGACTTCGTGGAGGGCCTGCGT; GTCTTTGACAAGGAGAGCAATGGCACGGTCATGGGTGCTGAGCTTCGGCACGTCCTTGCC; ACCCTGGGAGAGAAGATGACTGAGGCTGAAGTGGAGCAGCTGTTAGCTGGGCAAGAGGAT; GCCAATGGCTGCATCAATTATGAAGCCTTTGTCAAGCACATCATGTCAGGGTGA; ; Clone variation with respect to NM_002476.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002476 unedited; NTTCATTACCCCGCCCGTTGNAGCAAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTATAT; AAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCC; GCGAATTCGGCACCAGGACGCCACGTCTCTCGGTTTCTTCTTAGATCACTCCTCTGCCAA; AGATCCCAACAAGACAACATGGCTCCCAAGAAGCCTGAGCCTAAGAAGGAGGCAGCCAAG; CCAGCTCCAGCTCCAGCTCCAGCCCCTGCACCAGCCCCTGCCCCAGCTCCTGAGGCTCCC; AAGGAACCTGCCTTTGACCCCAAGAGTGTAAAGATAGACTTCACTGCCGACCAGATTGAA; GAGTTCAAAGAGGCCTTTTCATTGTTTGACCGGACCCCGACTGGAGAGATGAAGATCACC; TACGGCCAGTGCGGNGATGTACTGCGGGCCCTGGGCCAGAACCCTACCAATGCCGAGGTG; CTGCGTGTGCTGGGCAAGCCAAGCCTGAGAGATGAATGTCAAGATGCTGGACTTTGAGAC; GTTCTTGCCCATCCTGCAGCACATTTTCCGCAACAGGGAGCAGGGCACCTATGAGGACTT; CGTGGAGGGCCTGCGTGTCTTTGACCAGGGAGAGCATGGCACGGTCATGGGGTGCTGAGC; TTCGGCACGTCCTTTGCCACCCTGGNAGAAGAAATGACTGAGGGCTGAGTGGACNCACTG; TTANCTGGGCCAGAGGATGCCAAGGGCTGCATCAATTATTGAAGCCTTTTCCAGCACATC; AGGTCCGGGTGAAACAAAATCCTTCAGGTGCTGGGCCTTGGCTTTAACCCTACCAGGGTG; ATTAAAAAAAGGCCCGGGCTGGTGAACCGAAAGGGAACCCCGAATATAACCCACCCCGTG; CCA;
• Restriction Sites: NotI-NotI
• ACCN: NM_002476
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002476.2, NP_002467.1
• RefSeq Size: 855 bp
• RefSeq ORF: 594 bp
• Locus ID: 4635
• UniProt ID: P12829
• Cytogenetics: 17q21.32
• Summary: Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) lacks the 5' exon but has an alternate 5' end, as compared to variant 1.