MLC1SA (MYL6B) (NM_002475) Human Untagged Clone

CAT#: SC118607

MYL6B (untagged)-Human myosin, light chain 6B, alkali, smooth muscle and non-muscle (MYL6B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MLC1SA
• Synonyms: MLC1SA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118607 sequence for NM_002475 edited (data generated by NextGen Sequencing)
  ATGCCTCCCAAGAAGGATGTTCCCGTGAAGAAACCAGCAGGGCCCTCCATCTCCAAACCT
  GCTGCTAAGCCAGCAGCAGCAGGGGCTCCTCCAGCCAAGACCAAAGCTGAGCCAGCTGTC
  CCCCAGGCCCCTCAGAAAACCCAGGAGCCTCCAGTCGATCTCTCCAAAGTGGTGATCGAG
  TTTAACAAGGACCAGCTGGAGGAGTTCAAGGAGGCCTTCGAGCTGTTTGACCGAGTGGGG
  GATGGCAAGATCCTGTACAGCCAGTGTGGGGACGTGATGAGGGCCCTGGGCCAGAACCCC
  ACCAACGCCGAGGTGCTCAAGGTCCTGGGGAACCCCAAGAGTGATGAGCTGAAGTCGCGG
  CGTGTGGACTTTGAGACTTTCCTGCCCATGCTCCAGGCAGTGGCCAAGAACCGAGGCCAA
  GGCACATATGAGGACTACTTGGAGGGGTTTCGTGTGTTTGACAAGGAGGGGAACGGCAAA
  GTCATGGGAGCAGAGCTCAGACATGTTCTCACCACCCTTGGAGAGAAGATGACTGAGGAG
  GAGGTGGAGACCGTTCTGGCAGGACACGAGGACAGCAACGGCTGCATCAACTACGAGGCC
  TTCTTGAAACACATCCTAAGCGTCTGA
  
  Clone variation with respect to NM_002475.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002475 unedited
  GAACGTCAAATTTTGTATACCATCATATAGGGCGGCCGCGAATTCGCACGAGGGAGATGG
  GTGCCCCCATCCGCACACTGTCCTTTGGCCACCGGACATCATGCCTCCCAAGAAGGATGT
  TCCCGTGAAGAAACCAGCAGGGCCCTCCATCTCCAAACCTGCTGCTAAGCCAGCAGCAGC
  AGGGGCTCCTCCAGCCAAGACCAAAGCTGAGCCAGCTGTCCCCCAGGCCCCTCAGAAAAC
  CCAGGAGCCTCCAGTCGATCTCTCCAAAGTGGTGATCGAGTTTAACAAGGACCAGCTGGA
  GGAGTTCAAGGAGGCCTTCGAGCTGTTTGACCGAGTGGGGGATGGCAAGATCCTGTACAG
  CCAGTGTGGGGACGTGATGAGGGCCCTGGGCCAGAACCCCACCAACGCCGAGGTGCTCAA
  GGTCCTGGGGAACCCCAAGAGTGATGAGCTGAAGTCGCGGCGTGTGGACTTTGAGACTTT
  CCTGCCCATGCTCCAGGCAGTGGCCAAGAACCGAGGCCAAGGCACATATGAGGACTACTT
  GGAGGGGTTTCGTGTGTTTGACAAGGAGGGGAACGGCAAAGTCATGGGAGCAGAGCTCAG
  ACATGTTCTCACCACCCTTGGAGAGAAGATGACTGAGGAGGAGGTGGAGACCGTTCTGGC
  AGGACACGAGGACAGCAACGGCTGCATCAACTACGAGGCCTTCTTGAAACACATCCTAAG
  CGTCTGAGTGCTGCAGATCCAGTGGGGTCCGGACACTGGGCCCCCGCAGCGAAAGCACGT
  TCCAGCCACCAGNAGGCCACCTATTGTTTCAAAATAAAGACTGGGTTCCTCTCTTTGGAA
  AAAANNNNNNGNAGGNAANNAAAGAAAAAANAAAAGAGAGANNGAAAAAGAGAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002475 unedited
  GGTGGAGCCCATCTTCTTTTTAAACATGGTGGCCTCCTGGTGGCTGGAACGTGCTTTCGC
  CTGCGGGGCCCAGTGTCCGGACCCCACTGGATCTGCAGCACTCAGACGCTTAGGATGTGT
  TTCAAGAAGGCCTCGTAGTTGATGCAGCCGTTGCTGTCCTCGTGTCCTGCCAGAACGGTC
  TCCACCTCCTCCTCAGTCATCTTCTCTCCAAGGGTGGTGAGAACATGTCTGAGCTCTGCT
  CCCATGACTTTGCCGTTCCCCTCCTTGTCAAACACACGAAACCCCTCCAAGTAGTCCTCA
  TATGTGCCTTGGCCTCGGTTCTTGGCCACTGCCTGGAGCATGGGCAGGAAAGTCTCAAAG
  TCCACACGCCGCGACTTCAGCTCATCACTCTTGGGGTTCCCCAGGACCTTGAGCACCTCG
  GCGTTGGTGGGGTTCTGGCCCAGGGCCCTCATCACGTCCCCACACTGGCTGTACAGGATC
  TTGCCATCCCCCACTCGGTCAAACAGCTCGAAGGCCTCCTTGAACTCCTCCAGCTGGTCC
  TTGTTAAACTCGATCACCACTTTGGAGAGATCGACTGGAGGCTCCTGGGCTTTCTGAGGG
  GCCTGGGGGACAGCTGGCTCCACCTTGGTCTTGCCTGGAGGACCCCCTGCTGCTGCTGGC
  TTCACCCCCGGTTCGGCGATGGCGGGCCCTGCTGGTTTCCTTCCCCGTACCATCCCTCTC
  TGGGTGGCCTGCTGTTCCCGGGGCCCCCCCGACACGTCGCCGGCTGGGGGTCCCTCTTCT
  CCTTTGTGTTCAAATTCTCGCCCCCCCTTTCTCTGTTTCTGTCTCCATATTTCTCGCGGC
  CCCCTTTAATCTTCTCTGCTTTTTTCGACCTTCCCCGTTCCTCCCCTATCGCCCTTTTTC
  CGTTCCGTGGCCCGCGGCTTCTCCCCCTTTCCGAACAACCCCAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002475
• Insert Size: 850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002475.2, NP_002466.1
• RefSeq Size: 778 bp
• RefSeq ORF: 627 bp
• Locus ID: 140465
• UniProt ID: P14649
• Cytogenetics: 12q13.2
• Domains: EFh
• Protein Pathways: Vascular smooth muscle contraction
• Gene Summary: Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in both slow-twitch skeletal muscle and in nonmuscle tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.