MLC1SA (MYL6B) (NM_002475) Human Untagged Clone

SKU SC118607

MYL6B (untagged)-Human myosin, light chain 6B, alkali, smooth muscle and non-muscle (MYL6B), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: MLC1SA
• Synonyms: MLC1SA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118607 sequence for NM_002475 edited (data generated by NextGen Sequencing); ATGCCTCCCAAGAAGGATGTTCCCGTGAAGAAACCAGCAGGGCCCTCCATCTCCAAACCT; GCTGCTAAGCCAGCAGCAGCAGGGGCTCCTCCAGCCAAGACCAAAGCTGAGCCAGCTGTC; CCCCAGGCCCCTCAGAAAACCCAGGAGCCTCCAGTCGATCTCTCCAAAGTGGTGATCGAG; TTTAACAAGGACCAGCTGGAGGAGTTCAAGGAGGCCTTCGAGCTGTTTGACCGAGTGGGG; GATGGCAAGATCCTGTACAGCCAGTGTGGGGACGTGATGAGGGCCCTGGGCCAGAACCCC; ACCAACGCCGAGGTGCTCAAGGTCCTGGGGAACCCCAAGAGTGATGAGCTGAAGTCGCGG; CGTGTGGACTTTGAGACTTTCCTGCCCATGCTCCAGGCAGTGGCCAAGAACCGAGGCCAA; GGCACATATGAGGACTACTTGGAGGGGTTTCGTGTGTTTGACAAGGAGGGGAACGGCAAA; GTCATGGGAGCAGAGCTCAGACATGTTCTCACCACCCTTGGAGAGAAGATGACTGAGGAG; GAGGTGGAGACCGTTCTGGCAGGACACGAGGACAGCAACGGCTGCATCAACTACGAGGCC; TTCTTGAAACACATCCTAAGCGTCTGA; ; Clone variation with respect to NM_002475.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002475 unedited; GAACGTCAAATTTTGTATACCATCATATAGGGCGGCCGCGAATTCGCACGAGGGAGATGG; GTGCCCCCATCCGCACACTGTCCTTTGGCCACCGGACATCATGCCTCCCAAGAAGGATGT; TCCCGTGAAGAAACCAGCAGGGCCCTCCATCTCCAAACCTGCTGCTAAGCCAGCAGCAGC; AGGGGCTCCTCCAGCCAAGACCAAAGCTGAGCCAGCTGTCCCCCAGGCCCCTCAGAAAAC; CCAGGAGCCTCCAGTCGATCTCTCCAAAGTGGTGATCGAGTTTAACAAGGACCAGCTGGA; GGAGTTCAAGGAGGCCTTCGAGCTGTTTGACCGAGTGGGGGATGGCAAGATCCTGTACAG; CCAGTGTGGGGACGTGATGAGGGCCCTGGGCCAGAACCCCACCAACGCCGAGGTGCTCAA; GGTCCTGGGGAACCCCAAGAGTGATGAGCTGAAGTCGCGGCGTGTGGACTTTGAGACTTT; CCTGCCCATGCTCCAGGCAGTGGCCAAGAACCGAGGCCAAGGCACATATGAGGACTACTT; GGAGGGGTTTCGTGTGTTTGACAAGGAGGGGAACGGCAAAGTCATGGGAGCAGAGCTCAG; ACATGTTCTCACCACCCTTGGAGAGAAGATGACTGAGGAGGAGGTGGAGACCGTTCTGGC; AGGACACGAGGACAGCAACGGCTGCATCAACTACGAGGCCTTCTTGAAACACATCCTAAG; CGTCTGAGTGCTGCAGATCCAGTGGGGTCCGGACACTGGGCCCCCGCAGCGAAAGCACGT; TCCAGCCACCAGNAGGCCACCTATTGTTTCAAAATAAAGACTGGGTTCCTCTCTTTGGAA; AAAANNNNNNGNAGGNAANNAAAGAAAAAANAAAAGAGAGANNGAAAAAGAGAGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002475 unedited; GGTGGAGCCCATCTTCTTTTTAAACATGGTGGCCTCCTGGTGGCTGGAACGTGCTTTCGC; CTGCGGGGCCCAGTGTCCGGACCCCACTGGATCTGCAGCACTCAGACGCTTAGGATGTGT; TTCAAGAAGGCCTCGTAGTTGATGCAGCCGTTGCTGTCCTCGTGTCCTGCCAGAACGGTC; TCCACCTCCTCCTCAGTCATCTTCTCTCCAAGGGTGGTGAGAACATGTCTGAGCTCTGCT; CCCATGACTTTGCCGTTCCCCTCCTTGTCAAACACACGAAACCCCTCCAAGTAGTCCTCA; TATGTGCCTTGGCCTCGGTTCTTGGCCACTGCCTGGAGCATGGGCAGGAAAGTCTCAAAG; TCCACACGCCGCGACTTCAGCTCATCACTCTTGGGGTTCCCCAGGACCTTGAGCACCTCG; GCGTTGGTGGGGTTCTGGCCCAGGGCCCTCATCACGTCCCCACACTGGCTGTACAGGATC; TTGCCATCCCCCACTCGGTCAAACAGCTCGAAGGCCTCCTTGAACTCCTCCAGCTGGTCC; TTGTTAAACTCGATCACCACTTTGGAGAGATCGACTGGAGGCTCCTGGGCTTTCTGAGGG; GCCTGGGGGACAGCTGGCTCCACCTTGGTCTTGCCTGGAGGACCCCCTGCTGCTGCTGGC; TTCACCCCCGGTTCGGCGATGGCGGGCCCTGCTGGTTTCCTTCCCCGTACCATCCCTCTC; TGGGTGGCCTGCTGTTCCCGGGGCCCCCCCGACACGTCGCCGGCTGGGGGTCCCTCTTCT; CCTTTGTGTTCAAATTCTCGCCCCCCCTTTCTCTGTTTCTGTCTCCATATTTCTCGCGGC; CCCCTTTAATCTTCTCTGCTTTTTTCGACCTTCCCCGTTCCTCCCCTATCGCCCTTTTTC; CGTTCCGTGGCCCGCGGCTTCTCCCCCTTTCCGAACAACCCCAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_002475
• Insert Size: 850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002475.2, NP_002466.1
• RefSeq Size: 778 bp
• RefSeq ORF: 627 bp
• Locus ID: 140465
• UniProt ID: P14649
• Cytogenetics: 12q13.2
• Domains: EFh
• Protein Pathways: Vascular smooth muscle contraction
• Summary: Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in both slow-twitch skeletal muscle and in nonmuscle tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.