GPNMB (NM_002510) Human Untagged Clone

SKU SC118567

GPNMB (untagged)-Human glycoprotein (transmembrane) nmb (GPNMB), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: GPNMB
• Synonyms: HGFIN; NMB; PLCA3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002510, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGAATGTCTCTACTATTTCCTGGGATTTCTGCTCCTGGCTGCAAGATTGCCACTTGATGCCGCCAAAC; GATTTCATGATGTGCTGGGCAATGAAAGACCTTCTGCTTACATGAGGGAGCACAATCAATTAAATGGCTG; GTCTTCTGATGAAAATGACTGGAATGAAAAACTCTACCCAGTGTGGAAGCGGGGAGACATGAGGTGGAAA; AACTCCTGGAAGGGAGGCCGTGTGCAGGCGGTCCTGACCAGTGACTCACCAGCCCTCGTGGGCTCAAATA; TAACATTTGCGGTGAACCTGATATTCCCTAGATGCCAAAAGGAAGATGCCAATGGCAACATAGTCTATGA; GAAGAACTGCAGAAATGAGGCTGGTTTATCTGCTGATCCGTATGTTTACAACTGGACAGCATGGTCAGAG; GACAGTGACGGGGAAAATGGCACCGGCCAAAGCCATCATAACGTCTTCCCTGATGGGAAACCTTTTCCTC; ACCACCCCGGATGGAGAAGATGGAATTTCATCTACGTCTTCCACACACTTGGTCAGTATTTCCAGAAATT; GGGACGATGTTCAGTGAGAGTTTCTGTGAACACAGCCAATGTGACACTTGGGCCTCAACTCATGGAAGTG; ACTGTCTACAGAAGACATGGACGGGCATATGTTCCCATCGCACAAGTGAAAGATGTGTACGTGGTAACAG; ATCAGATTCCTGTGTTTGTGACTATGTTCCAGAAGAACGATCGAAATTCATCCGACGAAACCTTCCTCAA; AGATCTCCCCATTATGTTTGATGTCCTGATTCATGATCCTAGCCACTTCCTCAATTATTCTACCATTAAC; TACAAGTGGAGCTTCGGGGATAATACTGGCCTGTTTGTTTCCACCAATCATACTGTGAATCACACGTATG; TGCTCAATGGAACCTTCAGCCTTAACCTCACTGTGAAAGCTGCAGCACCAGGACCTTGTCCGCCACCGCC; ACCACCACCCAGACCTTCAAAACCCACCCCTTCTTTAGGACCTGCTGGTGACAACCCCCTGGAGCTGAGT; AGGATTCCTGATGAAAACTGCCAGATTAACAGATATGGCCACTTTCAAGCCACCATCACAATTGTAGAGG; GAATCTTAGAGGTTAACATCATCCAGATGACAGACGTCCTGATGCCGGTGCCATGGCCTGAAAGCTCCCT; AATAGACTTTGTCGTGACCTGCCAAGGGAGCATTCCCACGGAGGTCTGTACCATCATTTCTGACCCCACC; TGCGAGATCACCCAGAACACAGTCTGCAGCCCTGTGGATGTGGATGAGATGTGTCTGCTGACTGTGAGAC; GAACCTTCAATGGGTCTGGGACGTACTGTGTGAACCTCACCCTGGGGGATGACACAAGCCTGGCTCTCAC; GAGCACCCTGATTTCTGTTCCTGACAGAGACCCAGCCTCGCCTTTAAGGATGGCAAACAGTGCCCTGATC; TCCGTTGGCTGCTTGGCCATATTTGTCACTGTGATCTCCCTCTTGGTGTACAAAAAACACAAGGAATACA; ACCCAATAGAAAATAGTCCTGGGAATGTGGTCAGAAGCAAAGGCCTGAGTGTCTTTCTCAACCGTGCAAA; AGCCGTGTTCTTCCCGGGAAACCAGGAAAAGGATCCGCTACTCAAAAACCAAGAATTTAAAGGAGTTTCT; TAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002510 unedited; NGTTCAAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTGGACGGG; CCCAGAGGAATTCAGAGTTAAACCTTGAGTGCCTGCGTCCGTGAGAATTCAGCATGGAAT; GTCTCTACTATTTCCTGGGATTTCTGCTCCTGGCTGCAAGATTGCCACTTGATGCCGCCA; AACGATTTCATGATGTGCTGGGCAATGAAAGACCTTCTGCTTACATGAGGGAGCACAATC; AATTAAATGGCTGGTCTTCTGATGAAAATGACTGGAATGAAAAACTCTACCCAGTGTGGA; AGCGGGGAGACATGAGGTGGAAAAACTCCTGGAAGGGAGGCCGTGTGCAGGCGGTCCTGA; CCAGTGACTCACCAGCCCTCGTGGGCTCAAATATAACATTTGCGGTGAACCTGATATTCC; CTAGATGCCAAAAGGAAGATGCCAATGGCAACATAGTCTATGAGAAGAACTGCAGAAATG; AGGCTGGTTTATCTGCTGATCCGTATGTTTACAACTGGACAGCATGGTCAGAGGACAGTG; ACGGGGAAAATGGCACCGGCCAAAGCCATCATAACGTCTTCCCTGATGGGAAACCTTTTC; CTCACCACCCCGGATGGAGAAGATGGAATTTCATCTACGTCTTCCACACACTTGGTCAGT; ATTTCCAGAAATTGGGACGATGTTCAGTGAGAGTTTCTGTGAACACAGCCAATGTGACAC; TTGNGCCTCAACTCATGGAAGTGACTGTCTACAGAAGACATGGACGGGCATATGTTCCCA; TCGCACAAGTGAAAGATGTGTACGTGGTAACAGATCAGATNCCTGTGTTTGTGACTATGT; TCCAGAAAGAACGATCGAATTCATCCGACGAAACCTTTCTCAAAGACTCCCCATTATGTT; GATGTCCTGATNCAGATCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002510 unedited; GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCTTTTTTTTTCAGGAGGGAAAA; AAAAAAAATGTCTTGTTTAACATTTAGAAAAGGGTTTTATTTCCAAACAGGGGGGAAATC; CTTTTTGATAAAGTTGCCTTAAAAAACCAGGGCCCTTTTTTTTGGAAAGTTTTAATTTTT; GATTTAAAATTTGGGCCGGGCCTGTCCAAAATTTTGGAAATTTCCCCAAAAGGGCCCCTC; ACCTTCTTGCCTGGTTTTTAAAAACCCCTACCTAAGGGCCCTTGGAAAAAAGGAATTTTT; GAATTTTTTTCTTTCACTCACCCAAACAAAGTAGGTTGTCCCCAAAATCCTCCCCCCCTT; TTTTGAAAGTATTTTTTTTTTTGAGTTTAACAGGGGGGCCCATTTTACCAAAGGTTTGGG; CCTGTTTTTGTCCCATCAGGAGTTCATGAGGGGGTTTTGAAGGGGGATTCATTTACCTTG; GAACATGATTTTAAAAACGGGCCTACTTTGGTTTTATGCTTCCAGTTCCTTGGAAAGCTG; AAAGTTTCCCCCCTTTTTTTGCAGTTAACCGGGGTAAGGCTTTAATCAATAGGAGTTTTT; TTCCTTACTTTGGGGACACAGGGTTTTTATCCTACTAGTTCCCTAAAACTTTTTAAAGGG; AAACTTAAAAATTATGAAAAAACCCCTGCCTAAGTGCTTTTATAAANTTCCCAACATGCC; TGAAGCTGCCTGCAGTTAAATCCCTCTCCCATTCTTTAATGACCTTTACCTTTAAAAATT; CCCTTCCCCAACCCAAATTCTTTTAACAATATTTTTAGAAAAAAGGTTATACCCCCTCCA; CCATTCCATTATG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002510
• Insert Size: 2740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002510.2, NP_002501.1
• RefSeq Size: 2739 bp
• RefSeq ORF: 1683 bp
• Locus ID: 10457
• UniProt ID: Q14956
• Cytogenetics: 7p15.3
• Domains: PKD
• Protein Families: Druggable Genome, Transmembrane
• Summary: The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.