PARN (NM_002582) Human Untagged Clone

CAT#: SC118566

PARN (untagged)-Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PARN
• Synonyms: DAN; DKCB6; PFBMFT4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002582, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGATAATCAGGAGCAATTTTAAGAGTAATCTTCACAAAGTGTACCAGGCCATAGAGGAGGCCGACT
  TCTTCGCCATCGATGGGGAGTTTTCAGGAATCAGTGATGGACCTTCAGTCTCTGCATTAACAAATGGTTT
  TGACACTCCAGAAGAGAGGTATCAGAAGCTTAAAAAGCATTCCATGGACTTTTTGCTATTTCAGTTTGGC
  CTTTGCACTTTTAAGTATGACTACACAGATTCAAAGTATATAACGAAGTCATTTAACTTCTATGTTTTCC
  CGAAACCCTTCAATAGATCCTCACCAGATGTCAAATTTGTTTGTCAGAGCTCCAGCATTGACTTTCTAGC
  AAGCCAGGGATTTGATTTTAATAAAGTTTTTCGAAATGGAATTCCATATTTAAATCAGGAAGAAGAAAGA
  CAGTTAAGAGAGCAGTATGATGAAAAACGTTCACAGGCGAATGGTGCAGGAGCTCTGTCCTATGTATCTC
  CTAACACTTCAAAATGTCCTGTCACGATTCCTGAGGATCAAAAGAAGTTTATTGACCAAGTGGTAGAGAA
  AATAGAGGATTTATTACAAAGTGAAGAAAACAAGAACTTGGATTTAGAGCCATGTACCGGGTTCCAAAGA
  AAACTAATTTATCAGACTTTGAGCTGGAAGTATCCGAAAGGCATTCATGTTGAGACTTTAGAAACTGAAA
  AGAAGGAGCGATATATAGTTATCAGCAAAGTAGATGAAGAAGAACGCAAAAGAAGAGAGCAGCAGAAACA
  TGCCAAAGAACAGGAGGAGCTGAATGATGCTGTGGGATTTTCTAGAGTCATTCACGCCATTGCTAATTCG
  GGAAAACTTGTTATTGGACACAATATGCTCTTGGACGTCATGCACACAGTTCATCAGTTCTACTGCCCTC
  TGCCTGCGGACTTAAGTGAGTTTAAAGAGATGACAACATGTGTTTTCCCCAGACTCTTGGATACTAAATT
  GATGGCCAGCACACAACCTTTTAAGGATATCATTAACAACACATCCCTTGCGGAATTGGAAAAGCGGTTA
  AAAGAGACACCTTTCAACCCTCCTAAAGTTGAAAGTGCCGAAGGTTTTCCAAGTTATGACACAGCCTCTG
  AACAACTCCACGAGGCAGGCTACGATGCCTACATCACAGGGCTGTGCTTCATCTCCATGGCCAATTACCT
  AGGTTCTTTTCTCAGCCCTCCAAAAATTCATGTGTCTGCCAGATCAAAACTCATTGAACCTTTTTTTAAC
  AAGTTATTTCTTATGAGGGTCATGGATATCCCCTATCTAAACTTGGAAGGACCAGACTTGCAGCCTAAAC
  GTGATCATGTTCTCCATGTGACATTCCCCAAAGAATGGAAAACCAGCGACCTTTACCAGCTTTTCAGTGC
  CTTTGGTAACATTCAGATATCCTGGATTGATGACACATCAGCATTTGTTTCCCTTAGCCAGCCCGAGCAA
  GTAAAGATTGCTGTCAATACCAGCAAATATGCAGAAAGCTATCGGATCCAAACCTATGCTGAATATATGG
  GGAGAAAACAGGAAGAGAAGCAGATCAAAAGAAAGTGGACTGAAGATAGCTGGAAGGAGGCTGACAGCAA
  ACGGTTAAACCCCCAGTGCATACCCTACACCCTGCAGAATCACTATTACCGCAACAATAGTTTTACAGCT
  CCCAGCACAGTAGGAAAGAGAAATTTGAGTCCTAGTCAAGAGGAAGCTGGCCTGGAGGACGGAGTGTCAG
  GGGAGATTTCCGACACTGAGCTTGAGCAGACCGATTCCTGTGCAGAGCCCCTCTCAGAGGGAAGGAAAAA
  GGCCAAGAAATTAAAAAGAATGAAGAAGGAGCTTTCTCCAGCAGGAAGCATCTCGAAGAACAGCCCTGCC
  ACACTCTTTGAAGTTCCTGACACATGGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002582 unedited
  CCTGTCGCATACGNATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGGGTGGGGC
  CAAGGTTCCGGCCACTCTGCAGAATGGAGATAATCAGGAGCAATTTTAAGAGTAATCTTC
  ACAAAGTGTACCAGGCCATAGAGGAGGCCGACTTCTTCGCCATCGATGGGGAGTTTTCAG
  GAATCAGTGATGGACCTTCAGTCTCTGCATTAACAAATGGTTTTGACACTCCAGAAGAGA
  GGTATCAGAAGCTTAAAAAGCATTCCATGGACTTTTTGCTATTTCAGTTTGGCCTTTGCA
  CTTTTAAGTATGACTACACAGATTCAAAGTATATAACGAAGTCATTTAACTTCTATGTTT
  TCCCGAAACCCTTCAATAGATCCTCACCAGATGTCAAATTTGTTTGTCAGAGCTCCAGCA
  TTGACTTTCTAGCAAGCCAGGGATTTGATTTTAATAAAGTTTTTCGAAATGGAATTCCAT
  ATTTAAATCAGGAAGAAGAAAGACAGTTAAGAGAGCAGTATGATGAAAAACGTTCACAGG
  CGAATGGTGCAGGAGCTCTGTCCTATGTATCTCCTAACACTTCAAAATGTCCTGTCACGA
  TTCCTGAGGATCAAAAGAAGTTTATTGACCAAGTGGTAGAGAAAATAGAGGATTTATTAC
  AAAGTGAAGAAAACAAGAACTTGGATTTAGAGCCATGTACCGGGTTCCAAAGAAAACTAA
  TTTATCAGACTNTGAGCTACTAGTATCCGAAAGGCATTCATGTTGAGACTTTAGAAACTG
  TAAAGAGGAGCGATATATAGTTATCAGCAAAGTAGATGAAGAAAGACGCCAAAGAAGAGA
  GCAGCAGAAACATGCCAAAGAACAGGAGGAGCTGAATGATGCCTGNGGGATTTNCTAGAG
  TCATTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002582 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTATGAAAATGTTTTTAA
  TTTTCATCTTTTGGAAATACATTTTTCATTTTTATTTCCACCATACAAAAATGTGAAATA
  TCTAACAATGATCTATCTGAAGCGGGTGGAGCAAAGCAGCGCCATGAGCGTTTGTCGTTG
  CTGTGATCTGTTTCAACGGAGAATGGGCTGGGACATGTTGTAGATTTGCACGATTTCACA
  CACACACACACACACACACACACACACACACACACACAGACACGTACGCACACACGCTGC
  CGTACCCCGAGACCGCCATCCAAACAAACGAACAGAGACTCTGGAAAGTGAACACAGCGC
  CACGCATAAGAACAGAAGTTAACCTTTTTACTCGTACATCCCCCATGAGAAACTCACGTC
  TTAGGAGAAAGGAACTCTACATAAATATGCCCAAAGGCCAGGGCATACGGCAAGCCCTCT
  CATGGGTGGGCATGAGTGGACATCTTCCTGAAGGAAGGACAAGCTTGAGAGCGTCATGTT
  TTCAGGCAGCTATGGTGGGAAGCAGGGAGGCACAACCGATTTTTCCACTGGGAGACTAAC
  CGAGGGCTGCAACTGCCTCAAAATCTCAGTGCGAGAAGATGCACAGGCAGGACAGTACAC
  CTCCAAATTCATGATCACAGCAGCTGGAATGTCAGCTTTTTTTGCAGATTTCACAGCCGA
  CACTCCCCATCAAGCAGGGTTCTTAAGCACACTGGGTCATGACAGGAAAGGCCTCACCAG
  AGCAACACGGTATTCACTGGTTAGCCCGTACACATTCTTGACACCCTACACCGGGATGAG
  TGTCATGTCACCAGCCGTTTAATTTAAAGGGGAAAAACCCGCCCCCAAATAAAACTGTTT
  TTCCCCCCCAGGAACACTGGGTTCCACCCCTCCATACCACATTGATA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002582
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002582.1, NP_002573.1
• RefSeq Size: 2984 bp
• RefSeq ORF: 1920 bp
• Locus ID: 5073
• UniProt ID: O95453
• Cytogenetics: 16p13.12
• Domains: R3H, CAF1
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• Gene Summary: The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).