Proprotein Convertase 2 (PCSK2) (NM_002594) Human Untagged Clone

CAT#: SC118531

PCSK2 (untagged)-Human proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Proprotein Convertase 2
• Synonyms: NEC-2; NEC 2; NEC2; PC2; SPC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118531 sequence for NM_002594 edited (data generated by NextGen Sequencing)
  ATGAAGGGTGGTTGTGTCTCCCAGTGGAAGGCGGCCGCCGGGTTCCTCTTCTGTGTCATG
  GTTTTTGCATCTGCTGAGCGACCGGTCTTCACGAATCATTTTCTTGTGGAGTTGCATAAA
  GGGGGAGAGGACAAAGCTCGCCAAGTTGCAGCAGAACACGGCTTTGGAGTCCGAAAGCTT
  CCCTTTGCTGAAGGTCTGTACCACTTTTATCACAATGGCCTTGCAAAGGCCAAGAGAAGA
  CGCAGCCTACACCACAAGCAGCAGCTGGAGAGAGACCCCAGGGTAAAGATGGCTTTGCAG
  CAGGAAGGATTTGACCGAAAAAAGCGAGGTTACAGAGACATCAATGAGATCGACATCAAC
  ATGAACGATCCTCTTTTTACAAAGCAGTGGTATCTGATCAATACTGGGCAAGCTGATGGC
  ACTCCTGGCCTTGATTTGAATGTGGCTGAAGCCTGGGAGCTGGGATACACAGGGAAAGGT
  GTTACCATTGGAATTATGGATGATGGGATTGACTATCTCCACCCGGACCTGGCCTCCAAC
  TATAATGCCGAAGCAAGTTACGACTTCAGCAGCAACGACCCCTATCCTTACCCTCGGTAC
  ACAGATGACTGGTTTAACAGCCACGGGACCCGATGTGCAGGAGAAGTTTCTGCTGCCGCC
  AACAACAATATCTGTGGAGTTGGAGTAGCATACAACTCCAAGGTTGCAGGCATCCGGATG
  CTGGACCAGCCATTCATGACAGACATCATCGAGGCCTCCTCCATCAGTCATATGCCACAG
  CTGATTGACATCTACAGCGCCAGCTGGGGCCCCACAGACAACGGCAAGACAGTGGATGGG
  CCCCGGGAGCTCACGCTGCAGGCCATGGCCGATGGCGTGAACAAGGGCCGCGGCGGCAAA
  GGCAGCATCTACGTGTGGGCCTCCGGGGACGGCGGCAGCTATGACGACTGCAACTGCGAC
  GGCTACGCCTCCAGCATGTGGACCATCTCCATCAACTCAGCCATCAACGACGGCAGGACT
  GCCCTGTACGACGAGAGCTGCTCTTCCACCTTGGCTTCCACCTTCAGCAACGGGAGGAAA
  AGGAACCCCGAGGCCGGTGTGGCAACCACAGATTTGTACGGCAACTGCACTCTGAGGCAT
  TCTGGGACATCTGCAGCTGCCCCCGAGGCAGCTGGTGTGTTTGCACTGGCTCTGGAGGCT
  AACCTGGGTCTGACCTGGCGGGACATGCAGCATCTGACTGTGCTCACCTCCAAACGGAAC
  CAGCTTCACGACGAGGTCCATCAGTGGCGGCGCAATGGGGTCGGCCTGGAATTTAATCAC
  CTCTTTGGCTACGGGGTCCTTGATGCAGGTGCCATGGTGAAAATGGCTAAAGACTGGAAA
  ACCGTGCCTGAGAGATTCCACTGTGTGGGAGGCTCCGTGCAGGACCCTGAGAAAATACCA
  TCCACTGGCAAGTTGGTGCTGACACTCACAACCGACGCCTGTGAGGGGAAGGAAAATTTT
  GTCCGCTACCTGGAGCATGTCCAGGCTGTCATCACGGTCAACGCAACCAGAAGAGGAGAC
  CTGAACATCAACATGACTTCCCCTATGGGCACCAAGTCCATTTTGCTGAGCCGGCGTCCA
  AGGGATGACGACTCCAAGGTGGGCTTTGACAAGTGGCCTTTCATGACCACTCACACGTGG
  GGGGAAGACGCCCGAGGCACCTGGACCCTGGAGCTGGGATTTGTCGGCAGCGCCCCGCAG
  AAGGGGGTGCTGAAGGAGTGGACCCTGATGCTGCATGGCACTCAGAGTGCCCCGTACATC
  GACCAGGTGGTGCGGGATTACCAGTCCAAGTTGGCCATGTCCAAGAAAGAGGAGCTGGAG
  GAAGAGCTGGACGAAGCCGTGGAGAGAAGCCTGAAAAGCATCCTTAACAAGAACTAG
  
  Clone variation with respect to NM_002594.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002594 unedited
  GGAATTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGCAGCTCCCCA
  CATTCGCACCCCTGCCCGCGCGCCGGGCCGCCTGACTGCACGGCTTCCCCTCCAGCCAGA
  TGCTGGAGAACACATACTGATTCGCTGCTTTCCAAGACCCTGTTCAGTCTCTTTCTCTAT
  ACAAAGATTTTTTTAAAAACTATATATAAGAATTCTTTATTTGCACCCTCCCTCCGAGTC
  CCCTGCTCCGCCAGCCTGCGCGCCTCCTAGCACCACTTTTCACTCCCAAAGAAGGATGAA
  GGGTGGTTGTGTCTCCCAGTGGAAGGCGGCCGCCGGGTTCCTCTTCTGTGTCATGGTTTT
  TGCATCTGCTGAGCGACCGGTCTTCACGAATCATTTTCTTGTGGAGTTGCATAAAGGGGG
  AGAGGACAAAGCTCGCCAAGTTGCAGCAGAACACGGCTTTGGAGTCCGAAAGCTTCCCTT
  TGCTGAAGGTCTGTACCACTTTTATCACAATGGCCTTGCAAAGGCCAAGAGAAGACGCAG
  CCTACACCACAAGCAGCAGCTGGAGAGAGACCCCAGGGTAAAGATGGCTTTGCAGCAGGA
  AGGATTTGACCGAAAAAAGCGAGGTTACAGAGACATCAATGGAGATCGACATCAACATNG
  AACGANTCCTCTTTTTACAANAGCAGTGGGGTATCTGATNCAATACTGGGGCAAGCTGAT
  GGGCACTCCCTGGCCCTTGATTTTGAATGGTGGGCTGAAAGCCTGGNGAGCTGNGATACC
  AGGNNAAAAGGGTGNTACCATTGGAATNATGGGATGAAGGGGGAATGACTATTCTCCACC
  CCGGACCCTGGCCTTCAACTATATGGCGAAGGCAAGTTACGACTTCAGAGNNAACGACCC
  CTATTCTTACCCTCGTCACACGAAGAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002594 unedited
  CGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGTTGGGAACGCTATATTT
  GGGGCATAGAGTAAAAAAGATTGTTTCCATTGTAATGAAAATAATCATTGACAGTGAGTG
  AAGCTTCAGATTAAGAAGACGGAATTGCCTGTACGGGTGATGGAATTGCTAGGTGCCTGC
  CTGAAACGTGGAGCGAGGACAGAGGCGGAGCTGGGGAGGGAGGGCGGTGGGAAAGGCGGA
  TGTGCAGCGCTAGTTCTTGTTAAAGATGCTTTTCAAGCTTCTCTCCACGGCTTCGTCCAG
  CTCTTCCTCCAGCTCCTCTTTCTTGGACATGGCCAACTTGGACTGGTAATCCCGCACCAC
  CTGGTCGATGTACGGGGCACTCTGAGTGCCATGCAGCATCAGGGTCCACTCCTTCAGCAC
  CCCCTTCTGCGGGGCGCTGCCGACAAATCCCAGCTCCAGGGTCCAGGTGCCTCGGGCGTC
  TTCCCCCCACGTGTGAGTGGTCATGAAAGGCCACTTGTCAAAGCCCACCTTGGAGTCGTC
  ATCCCTTGGACGCCGGCTCATCAAAATGGACTTGGTGCCCATAGGGGAAGTCATGTTGAT
  GTTCAAGTCTCCTCTTCTGGTTGCGTTGACCGTGATGACAGCCTGGACATGCTCCAGGTA
  GCGGACAAAATTTTCCTTCCCCTCACAGGCGTCNGTTGTGAGTGTCAGCACCAACTTGCC
  AGTGGATGGTATTTTCTCAAGGTCCTGCACGGAGCCTCCCCACAGTGGAATCTCTCATGC
  ACGTTTTCCAGTCTTTAGCATTTTCACATGGCACCTGCTCAAGGACCCGTACCAAAGAGT
  GATTAATTTCAGGCGACCCCATGGCCGCCCTGATGACCTCGTCGGAAACTGGTCCGTTGN
  AGTAAGCACATAGAGCTGATGTCCGCAGTCAAACCAGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002594
• Insert Size: 2460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002594.2, NP_002585.2
• RefSeq Size: 4745 bp
• RefSeq ORF: 1917 bp
• Locus ID: 5126
• UniProt ID: P16519
• Cytogenetics: 20p12.1
• Domains: Peptidase_S8, P
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The protein undergoes an initial autocatalytic processing event and interacts with a neuroendocrine secretory protein in the ER, exits the ER and sorts to secretory granules, where it is cleaved and catalytically activated during intracellular transport. The encoded protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Single nucleotide polymorphisms in this gene may increase susceptibility to myocardial infarction and type 2 diabetes. This gene may also play a role in tumor development and progression. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.