PBX1 (NM_002585) Human Untagged Clone

SKU SC118523

PBX1 (untagged)-Human pre-B-cell leukemia homeobox 1 (PBX1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PBX1
• Synonyms: CAKUHED
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118523 sequence for NM_002585 edited (data generated by NextGen Sequencing); ATGGACGAGCAGCCCAGGCTGATGCATTCCCATGCTGGGGTCGGGATGGCCGGACACCCC; GGCCTGTCCCAGCACTTGCAGGATGGGGCCGGAGGGACCGAGGGGGAGGGCGGGAGGAAG; CAGGACATTGGAGACATTTTACAGCAAATTATGACCATCACAGACCAGAGTTTGGATGAG; GCGCAGGCCAGAAAACATGCTTTAAACTGCCACAGAATGAAGCCTGCCTTGTTTAATGTG; TTGTGTGAAATCAAAGAAAAAACAGTTTTGAGTATCCGAGGAGCCCAGGAGGAGGAACCC; ACAGACCCCCAGCTGATGCGGCTGGACAACATGCTGTTAGCGGAAGGCGTGGCGGGGCCT; GAGAAGGGCGGAGGGTCGGCGGCAGCGGCGGCAGCGGCGGCGGCTTCTGGAGGGGCAGGT; TCAGACAACTCAGTGGAGCATTCAGATTACAGAGCCAAACTCTCACAGATCAGACAAATC; TACCATACGGAGCTGGAGAAATACGAGCAGGCCTGCAACGAGTTCACCACCCACGTGATG; AATCTCCTGCGAGAGCAAAGCCGGACCAGGCCCATCTCCCCAAAGGAGATTGAGCGGATG; GTCAGCATCATCCACCGCAAGTTCAGCTCCATCCAGATGCAGCTCAAGCAGAGCACGTGC; GAGGCGGTGATGATCCTGCGTTCCCGATTTCTGGATGCGCGGCGGAAGAGACGGAATTTC; AACAAGCAAGCGACAGAAATCCTGAATGAATATTTCTATTCCCATCTCAGCAACCCTTAC; CCCAGTGAGGAAGCCAAAGAGGAGTTAGCCAAGAAGTGTGGCATCACAGTCTCCCAGGTA; TCAAACTGGTTTGGAAATAAGCGAATCCGGTACAAGAAGAACATAGGTAAATTTCAAGAG; GAAGCCAATATTTATGCTGCCAAAACAGCTGTCACTGCTACCAATGTGTCAGCCCATGGA; AGCCAAGCTAACTCGCCCTCAACTCCCAACTCGGCTGGTTCTTCCAGTTCTTTTAACATG; TCAAACTCTGGAGATTTGTTCATGAGCGTGCAGTCACTCAATGGGGATTCTTACCAAGGG; GCCCAGGTTGGAGCCAACGTGCAATCACAGGTGGATACCCTTCGCCATGTTATCAGCCAG; ACAGGAGGATACAGTGATGGACTCGCAGCCAGTCAGATGTACAGTCCGCAGGGCATCAGT; GCTAATGGAGGTTGGCAGGATGCTACTACCCCTTCATCAGTGACCTCCCCTACAGAAGGC; CCTGGCAGTGTTCACTCTGATACCTCCAACTGA; ; Clone variation with respect to NM_002585.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002585 unedited; GGGTGCAAATTTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGGCTTGAA; GGATAAAAAGCCTTGGTGCTTCCCAGGAGCCGAGCCGAGGAGCAGAAGAGGAAGAGCCGG; GGGCTGCCGTAGCCTTTGGAGATGGACGAGCAGCCCAGGCTGATGCATTCCCATGCTGGG; GTCGGGATGGCCGGACACCCCGGCCTGTCCCAGCACTTGCAGGATGGGGCCGGAGGGACC; GAGGGGGAGGGCGGGAGGAAGCAGGACATTGGAGACATTTTACAGCAAATTATGACCATC; ACAGACCAGAGTTTGGATGAGGCGCAGGCCAGAAAACATGCTTTAAACTGCCACAGAATG; AAGCCTGCCTTGTTTAATGTGTTGTGTGAAATCAAAGAAAAAACAGTTTTGAGTATCCGA; GGAGCCCAGGAGGAGGAACCCACAGACCCCCAGCTGATGCGGCTGGACAACATGCTGTTA; GCGGAAGGCGTGGCGGGGCCTGAGAAGGGCGGAGGGTCGGCGGCAGCGGCGGCAGCGGCG; GCGGCTTCTGGAGGGGCAGGTTCAGACAACTCAGTGGAGCATTCAGATTACAGAGCCAAA; CTCTCACAGATCAGACAAATCTACCATACGGAGCTGGAGAAATACGAGCAGGCCTGCAAC; GAGTTCACCACCCACGTGATGAATCTCCTGCGAGAGCAAGCCNGACCAGGCCCATCTCCC; CAAAGGAGATTGAGCGGATGGTCAGCATCATCCACCGCAAGTTCAGCTCCATCCAGATGC; AGCTCAAGCAGAGCACGTGCGAAGCGGTGATGATCCTGCNGTCCCGATTTCTGGATGCGN; CGCNGNAGAGACGGNATTTCAACAGCAGCGACAGAAATCCTGAATGAATATTNTATTCCC; ATCTCAGCACCCTTACCCAGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002585 unedited; NNNGGGATATATCTATGNNACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTTT; TTTTAGTTTACAAAATGAATTACTGTCACTTTTAAACATTGTGAAACAAGAAATGGATGT; GCACAACTCGACACTTTTCTAGCATTCTTGAACTAATTCACAAATGCAAGAAAATAAAAG; AAAAATGAGGGAAATGATGAATGTAGGTAGTCTGGTGTTAAAAACTAATGCAGGAATGAT; GCGCATTGTCACAGAAAGAAGAGGGGAAATTCCCCATCCTGTTTTGCGTGCTATGAGGGT; CATTTTTAAAATTTTTATTATAAACACTATTAAATTCAGACCGCTTTTTTGGTTTTTTTT; TTCTTTATCTGAATATACAAGAACATTCATTATCAAATGTTCATCATCAATACTACAAAG; AACGAGACACAAGTCGCAAGAAACAATGGAAAATGTTAATAAAGCTGAAAGAATCGTTGA; GTATTTTTTTGTTTCTTTTAAAGTTTTTTTTAAATTTGAGTTTCTGTAGTTTCATCTTTT; TTGGTATCGAAGTCAGGTTTTTCTGGGCAGAAAAACAAAGAGCAGGAAAGAGAGAGACAG; AGAGAAAGATGTGTGAGAAAAGACAACCAGCAAATAAAGAATCACCACCATAGCAAAATA; AAACATAAATTGAAAGAAGGTGCCACTTGGCAAATGTACTGCACGGGCGTCAATTCCATG; CCAGAGGGAGGATGAATTAGGGATGAAAAGGAGGAGATAAGGAAGAGTTACAAGGTGGGG; CAAACCCANGGTCTAGTATCGTTTCTCAGGGGAAAATCGCTGTCGGGGAAAATGGAGCAC; TCCCACCTCTATTAGAAAATGAGAAAATCCTGGGTGCATCTTGCTCTCTGGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_002585
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002585.1, NP_002576.1
• RefSeq Size: 1819 bp
• RefSeq ORF: 1293 bp
• Locus ID: 5087
• UniProt ID: P40424
• Cytogenetics: 1q23.3
• Domains: homeobox, PBX
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Summary: This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis and required for skeletal patterning and programming. A chromosomal translocation, t(1;19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]; Transcript Variant: This variant (1) represents the predominant transcript and encodes the longest isoform (1, also known as isoform PBX1a).