DNA Polymerase beta (POLB) (NM_002690) Human Untagged Clone

SKU SC118518

POLB (untagged)-Human polymerase (DNA directed), beta (POLB)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DNA Polymerase beta
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118518 sequence for NM_002690 edited (data generated by NextGen Sequencing); ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA; GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA; AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG; AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA; AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG; ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA; ACACTAGAAGATCTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTG; AAATATTTTGGGGACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGAT; ATTGTACTAAATGAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGT; TTCAGAAGAGGTGCAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTC; ACTTCAGAATCAACCAAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGTTACAAAAG; GTTCATTTTATCACAGATACCCTGTCAAAGGGTGAGACAAAGTTCATGGGTGTTTGCCAG; CTTCCCAGTAAAAATGATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATA; CCCAAAGATCAGTATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAG; AATATGAGGGCTCATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCC; TTGGGAGTCACTGGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTT; GATTACATCCAGTGGAAATACCGGGAACCCAAGGACCGGAGCGAATGA; ; Clone variation with respect to NM_002690.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002690 unedited; CCCCTGTTAGAGTTGAATCTCGACTCATATAGGCGGCCGCTGAACGGCACGAGGCCGGCA; CTGGGTTGCTCCTGCTCCCGTCTCCAAGTCCTGGTACCTCCTTCAAGCTGGGAGAGGGCT; CTAGTCCCTGGTTCTGAACACTCTGGGGTTCTCGGGTGCAGGCCGCCATGAGCAAACGGA; AGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACAGAACTCGCAAACT; TTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGAAAAGCAGCATCTG; TTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAGAAATTGCCTGGAG; TAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGAAAATTACGTAAAC; TGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTGACTCGAGTTAGTG; GCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAAACACTAGAAGATC; TCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTGAAATATTTTGGGG; ACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAAATG; AAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGTG; CAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCACCCAGCTTCACTTCAGAATCAAC; CAAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGCTACAAAGGGTTCATTTATCACA; CATACCCCTGCCAAGGGTGAGACAAAGCTCATGGGTGTTTGCCAGCTTCCCAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002690 unedited; AACCTTCCAGGGCCAGGNANAGCACTGGGGNAGGGGGTCACAGGGNATGCCACCCGGGAT; CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT; TTTAATAGTACATGAGGTTTTATTGACTACATTGCAAGCAAAAGCATGAAGAAGAAACAA; TCATTTAAAAACACCAAAGACCCTTACATAGCAAAGGTTAAGAAATAAATTAAGACTCCT; ATTGGGTTGTGTCTGCCAGGGAGGATACAGGCCTCATTCGCTCCGGTCCTTGGGTTCCCG; GTATTTCCACTGGATGTAATCAAAGATGTCTTTTTCACTATCCACTGGCAGGGGTTCTCC; TGCAACTCCAGTGACTCCCAAGGGACGGATGGTGTACTCATTGATTGTGAAACCCTTTTC; TAGGGCATGAGCCCTCATATTCTTATTGAAAATATCACTCCCAGTGAAATAGAGAACACC; ACAGTAATACTGATCTTTGGGTATCAACCTGATATCAATTCTTCTGTGTGGATATTCTTT; TTCATCATTTTTACTGGGAAGCTGGCAAACACCCATGAACTTTGTCTCACCCTTTGACAG; GGTATCTGTGATAAAATGAACCTTTTGTAACTGCTCCACAACCTGATGTAACAGTTTTGG; CTGTTTGGTTGATTCTGAAGTGAAGCTGGGATGGGTCAGGAGAACATCCATGTCACCACT; GGACTCTGCACCTCTTCTGAAACTGCCACAGACTGTAGCAATGTATTCAGAATCCACTTT; TTTAACTTCATTTAGTACAATATCTTGCATTTGTAACATCTCTTCACGAGGAATNCTTTT; NTNCAAGTCCCCAAATATNTCAGCCCAATTCGCTGAGATGGTCAATTATCTTCATTTTNC; TGAATCTTCTAGGTTTAATTCTTATTAACAACTNCTGCAGAGAGGCCATGCCCTACTCCG; TCAGAATGATGATGACTCNTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_002690
• Insert Size: 1250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002690.1, NP_002681.1
• RefSeq Size: 1259 bp
• RefSeq ORF: 1008 bp
• Locus ID: 5423
• UniProt ID: P06746
• Cytogenetics: 8p11.21
• Domains: HhH1, POLXc
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Summary: The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]