Pinin (PNN) (NM_002687) Human Untagged Clone

SKU SC118515

PNN (untagged)-Human pinin, desmosome associated protein (PNN)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Pinin
• Synonyms: DRS; DRSP; memA; SDK3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002687, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGTCGCCGTGAGAACTTTGCAGGAACAGCTGGAAAAGGCCAAAGAGAGTCTTAAGAACGTGGATG; AGAACATTCGCAAGCTCACCGGGCGGGATCCGAATGACGTGAGGCCCATCCAAGCCAGATTGCTGGCCCT; TTCTGGTCCTGGTGGAGGTAGAGGACGTGGTAGTTTATTACTGAGGCGTGGATTCTCAGATAGTGGAGGA; GGACCCCCAGCCAAACAGAGAGACCTTGAAGGGGCAGTCAGTAGGCTGGGCGGGGAGCGTCGGACCAGAA; GAGAATCACGCCAGGAAAGCGACCCGGAGGATGATGATGTTAAAAAGCCAGCATTGCAGTCTTCAGTTGT; AGCTACCTCCAAAGAGCGCACACGTAGAGACCTTATCCAGGATCAAAATATGGATGAAAAGGGAAAGCAA; AGGAACCGGCGAATATTTGGCTTGTTGATGGGTACCCTTCAAAAATTTAAACAAGAATCCACTGTTGCTA; CTGAAAGGCAAAAGCGGCGCCAGGAAATTGAACAAAAACTTGAAGTTCAGGCAGAAGAAGAGAGAAAGCA; GGTTGAAAATGAAAGGAGAGAACTGTTTGAAGAGAGGCGTGCTAAACAGACAGAACTGCGGCTTTTGGAA; CAGAAAGTTGAGCTTGCGCAGCTGCAAGAAGAATGGAATGAACATAATGCCAAAATAATTAAATATATAA; GAACTAAGACAAAGCCCCATTTGTTTTATATTCCTGGAAGAATGTGTCCAGCTACCCAAAAACTAATAGA; AGAGTCACAGAGAAAAATGAACGCTTTATTTGAAGGTAGACGCATCGAATTTGCAGAACAAATAAATAAA; ATGGAGGCTAGGCCTAGAAGACAATCAATGAAGGAAAAAGAGCATCAGGTGGTGCGTAATGAAGAACAGA; AGGCGGAACAAGAAGAGGGTAAGGTGGCTCAGCGAGAGGAAGAGTTGGAGGAGACAGGTAATCAGCACAA; TGATGTAGAAATAGAGGAAGCAGGAGAGGAAGAGGAAAAGGAAATAGCGATTGTTCATAGTGATGCAGAG; AAAGAACAGGAGGAGGAAGAACAAAAACAGGAAATGGAGGTTAAGATGGAGGAGGAAACTGAGGTAAGGG; AAAGTGAGAAGCAGCAGGATAGTCAGCCTGAAGAAGTTATGGATGTGCTAGAGATGGTTGAGAATGTCAA; ACATGTAATTGCTGACCAGGAGGTAATGGAAACTAATCGAGTTGAAAGTGTAGAACCTTCAGAAAATGAA; GCTAGCAAAGAATTGGAACCAGAAATGGAATTTGAAATTGAGCCAGATAAAGAATGTAAATCCCTTTCTC; CTGGGAAAGAGAATGTCAGTGCTTTAGACATGGAAAAGGAGTCTGAGGAAAAAGAAGAAAAAGAATCTGA; GCCCCAACCTGAGCCTGTGGCTCAACCTCAGCCTCAGTCTCAGCCCCAGCTTCAGCTTCAATCCCAGTCC; CAACCAGTACTCCAGTCCCAGCCTCCCTCTCAGCCTGAGGATTTGTCATTAGCTGTTTTACAGCCAACAC; CCCAAGTTACTCAGGAGCAAGGGCATTTACTACCTGAGAGGAAGGATTTTCCTGTAGAGTCTGTAAAACT; CACTGAGGTACCAGTAGAGCCAGTCTTGACAGTACATCCAGAGAGCAAGAGCAAAACCAAAACTAGGAGC; AGAAGTAGAGGTCGAGCTAGAAATAAAACAAGCAAGAGTAGAAGTCGAAGCAGTAGCAGTAGCAGTTCTA; GTAGCAGTTCAACCAGTAGCAGCAGTGGAAGTAGTTCCAGCAGTGGAAGTAGTAGCAGTCGCAGTAGTTC; CAGTAGCAGCTCCAGTACAAGTGGCAGCAGCAGCAGAGATAGTAGCAGTAGCACTAGTAGTAGTAGTGAG; AGTAGAAGTCGGAGTAGGGGCCGGGGACATAATAGAGATAGAAAGCACAGAAGGAGCGTGGATCGGAAGA; GAAGGGATACTTCAGGACTAGAAAGAAGTCACAAATCTTCAAAAGGTGGTAGTAGTAGAGATACAAAAGG; ATCAAAGGATAAGAATTCCCGGTCCGACAGAAAGAGGTCTATATCAGAGAGTAGTCGATCAGGCAAAAGA; TCTTCAAGAAGTGAAAGAGACCGAAAATCAGACAGGAAAGACAAAAGGCGTTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002687 unedited; ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCTGCCGCAGGGAGAAGAT; GGCGGTCGCCGTGAGAACTTTGCAGGAACAGCTGGAAAAGGCCAAAGAGAGTCTTAAGAA; CGTGGATGAGAACATTCGCAAGCTCACCGGGCGGGATCCGAATGACGTGAGGCCCATCCA; AGCCAGATTGCTGGCCCTTTCTGGTCCTGGTGGAGGTAGAGGACGTGGTAGTTTATTACT; GAGGCGTGGATTCTCAGATAGTGGAGGAGGACCCCCAGCCAAACAGAGAGACCTTGAAGG; GGCAGTCAGTAGGCTGGGCGGGGAGCGTCGGACCAGAAGAGAATCACGCCAGGAAAGCGA; CCCGGAGGATGATGATGTTAAAAAGCCAGCATTGCAGTCTTCAGTTGTAGCTACCTCCAA; AGAGCGCACACGTAGAGACCTTATCCAGGATCAAAATATGGATGAAAAGGGAAAGCAAAG; GAACCGGCGAATATTTGGCTTGTTGATGGGTACCCTTCAAAAATTTAAACAAGAATCCAC; TGTTGCTACTGAAAGGCAAAAGCGGCGCCAGGAAATTGAACAAAAACTTGAAGTTCAGGC; AGAAGAAGAGAGAAAGCAGGTTGAAAATGAAAGGAGAGAACTGTTTGAAGAGAGGCGTGC; TAAACAGACAGAACTGCGGCTTTTGGAACAGAAGTTGAGCTTGCGCAGCTGCAAGAAGAA; ATGGATGAACANTATGCCAANNATATTAAATATATAAGAACTAAGACCAAGCCCCCATTT; GTTTATATTCCTGGAAGAAATGTGTCCAGCTACCCAAAACTATNGGAAGAGTCCAGAGAA; AATGAACGCTTATTTTGAAGTAGACGCATCGAATTTGCAGACAAATAATAANATGGNAGG; CTGGNCCTAGAGAACATCATGGAGGAAAGAGCTCAGGTGGTGCGTATGAGAACCAGGCGA; CAAATAAGGTGAGGT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002687 unedited; GGCCGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTGGTAAACAAACAGTCTGTATTT; TCCAAAAAGATTTTTTACAACATGCAATTCTTAAGGCAGCATCCTCTTTACAAGGAAAGG; TAATCCTTTTTTATCAAGAAATCTTCTGCTGCAAAGAATGGCTAAGAAAGCCTGGCTTCT; TCCATTAACGCCTTTTGTCTTTCCTGTCTGATTTTCGGTCTCTTTCACTTCTTGAAGATC; TTTTGCCTGATCGACTACTCTCTGATATAGACCTCTTTCTGTCGGACCGGGAATTCTTAT; CCTTTGATCCTTTTGTATCTCTACTACTACCACCTTTTGAAGATTTGTGACTTCTTTCTA; GTCCTGAAGTATCCCTTCTCTTCCGATCCACGCTCCTTCTGTGCTTTCTATCTCTATTAT; GTCCCCGGCCCCTACTCCGACTTCTACTCTCACTACTACTACTAGTGCTACTGCTACTAT; CTCTGCTGCTGCTGCCACTTGTACTGGAGCTGCTACTGGAACTACTGCGACTGCTACTAC; TTCCACTGCTGGAACTACTTCCACTGCTGCTACTGGTTGAACTGCTACTAGAACTGCTAC; TGCTACTGCTTCGACTTCTACTCTTGCTTGTTTTATTTCTAGCTCGACCTCTACTTCTGC; TCCTAGTTTTGGTTTTGCTCTTGCTCTCTGAATGTACTGTCAAGACTGGCTCTACTGGTA; CCTCAGTGAGTTTTACAGACTCTACAGGAAAATCCCTCCTCTCANGTAGTAAATGCCCCT; GCTCCTGAGTAACTGGGGGGTGTGGCTGTTAAACAGCTAATGACAAATCCTCCAGCTGAA; AAGGGAGCCTGGACTGGATTACTGGTTGGGACTGGGATTGAACCTGAACCTGGGGCTGAN; ACTGNNAGCTGAGTTGAGCCACAGCCTCAGTTGGGGCTANATTCTTTTCTTCTCTTACCT; AAACTCCTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_002687
• Insert Size: 2380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002687.2, NP_002678.2
• RefSeq Size: 3606 bp
• RefSeq ORF: 3606 bp
• Locus ID: 5411
• UniProt ID: Q9H307
• Cytogenetics: 14q21.1
• Domains: pinin_SDK_memA, pinin_SDK_N
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Summary: Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.[UniProtKB/Swiss-Prot Function]