PGAM1 (NM_002629) Human Untagged Clone

CAT#: SC118510

PGAM1 (untagged)-Human phosphoglycerate mutase 1 (brain) (PGAM1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PGAM1
• Synonyms: HEL-S-35; PGAM-B; PGAMA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118510 sequence for NM_002629 edited (data generated by NextGen Sequencing)
  ATGGCCGCCTACAAACTGGTGCTGATCCGGCACGGCGAGAGCGCATGGAACCTGGAGAAC
  CGCTTCAGCGGCTGGTACGACGCCGACCTGAGCCCGGCGGGCCACGAGGAGGCGAAGCGC
  GGCGGGCAGGCGCTACGAGATGCTGGCTATGAGTTTGACATCTGCTTCACCTCAGTGCAG
  AAGAGAGCGATCCGGACCCTCTGGACAGTGCTAGATGCCATTGATCAGATGTGGCTGCCA
  GTGGTGAGGACTTGGCGCCTCAATGAGCGGCACTATGGGGGTCTAACCGGTCTCAATAAA
  GCAGAAACTGCTGCAAAGCATGGTGAGGCCCAGGTGAAGATCTGGAGGCGCTCCTATGAT
  GTCCCACCACCTCCGATGGAGCCCGACCATCCTTTCTACAGCAACATCAGTAAGGATCGC
  AGGTATGCAGACCTCACAGAAGATCAGCTACCCTCCTGTGAGAGTCTGAAGGATACTATT
  GCCAGAGCTCTGCCCTTCTGGAATGAAGAAATAGTTCCCCAGATCAAGGAGGGGAAACGT
  GTACTGATTGCAGCCCATGGCAACAGCCTCCGGGGCATTGTCAAGCATCTGGAGGGTCTC
  TCTGAAGAGGCTATCATGGAGCTGAACCTGCCGACTGGTATTCCCATTGTCTATGAATTG
  GACAAGAACTTGAAGCCTATCAAGCCCATGCAGTTTCTGGGGGATGAAGAGACGGTGCGC
  AAAGCCATGGAAGCTGTGGCTGCCCAGGGCAAGGCCAAGAAGTGA
  
  Clone variation with respect to NM_002629.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002629 unedited
  NGTCAGATATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCATCC
  CCAGCCCGCCGCCATGGCCGCCTACAAACTGGTGCTGATCCGGCACGGCGAGAGCGCATG
  GAACCTGGAGAACCGCTTCAGCGGCTGGTACGACGCCGACCTGAGCCCGGCGGGCCACGA
  GGAGGCGAAGCGCGGCGGGCAGGCGCTACGAGATGCTGGCTATGAGTTTGACATCTGCTT
  CACCTCAGTGCAGAAGAGAGCGATCCGGACCCTCTGGACAGTGCTAGATGCCATTGATCA
  GATGTGGCTGCCAGTGGTGAGGACTTGGCGCCTCAATGAGCGGCACTATGGGGGTCTAAC
  CGGTCTCAATAAAGCAGAAACTGCTGCAAAGCATGGTGAGGCCCAGGTGAAGATCTGGAG
  GCGCTCCTATGATGTCCCACCACCTCCGATGGAGCCCGACCATCCTTTCTACAGCAACAT
  CAGTAAGGATCGCAGGTATGCAGACCTCACAGAAGATCAGCTACCCTCCTGTGAGAGTCT
  GAAGGATACTATTGCCAGAGCTCTGCCCTTCTGGAATGAAGAAATAGTTCCCCAGATCAA
  GGAGGGNGAAACGTGTACTGATTGCAGCCCATGGCAACAGCCTCCGGGGCATTGTCAAGC
  ATCTGGGAGGGTCTCTCTGAAGAGGCTATCATGGAGCTGAACCTGCCGACTGGTATTCCC
  ATTGTCTATGAATTGGACAAGAACTTGAAGCCTATCAAGCCCATGCAGTTTCTGGGGGAT
  GAAGAGACGGTGCGCAAAGCCTGGGAGCTGTGGCTGCCCAGGCAGGCCAAGAAGTGAGGC
  CGGCGGGAGGATACTGTCCCAGAAGCAACCTCCTGCCCGTTTGGTCCTCTGCCCTCCACC
  TGCCATGTACACTGACACATCTGTAACTTTTTGAGTGTACTGCNACGGGGACAATGGCTT
  CATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002629 unedited
  ATGGCACTTCCAGGGCCGGNATAGCACTGGGGAGGGGTCACAGGNATGCCACCCGGGATC
  TGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT
  TTATTGCACATCACTCCTTTATTATACTGATATGGAAAAAGGATTTAGTACAGTTATGCT
  CAGATGAACACTGGACCCATGTGGCAGGGTCAAGCAACTAGAACATGATTCAGAAATCAG
  TGAAAGATACACTTGGACAGGACCAAGAGGCATTTCACTGCCATGAAACAAGGCAGGAAG
  GGATTCTAATACACACACCAGGAAGCACTCCTGCCCCTCAGAGGTCAAGGAGCTGATCCT
  ATATTGGTATGAGGAATGGCTTATTTTCTGATGACCACATGTGGGACTATTTCAACCGCC
  ACAAGAAACCCCAGAAGGGTTATTGTTTTGTATTATATATACTATACTTTTTTAATAAAA
  GTAAATTAACACATAACGAAATTCAGGATTGATCCCAACCTAGAGCCAGATCCTCTGGGG
  TCAGGGAGGAAACAGTTGTCACATCACCACGCAGGTTACATTCGTCTTCCACTGGAATGA
  CTAGAGCCCCCAGGCAGTAGGCAGTGACCTGACTGCAGAAGAGCAGAGGACAGACTCCGC
  TCATGGGGACAGACAGGCTCTGTTGCTTCTCCTCACTGGTCATGGCTTANCATGGTTCCT
  CCCCAAGTCCTTAGTAAACAAAGCACTCGCANAAACCCAAGTCACTACTTTTAAACTCTG
  TTGGATAAGGGGAGCTTTTCCATAGCTAGACTGAGAACCTGTGCTCTAGAACTGCTATTC
  TGACTANATGTATGAAAGGAAGTGGGTGCANGCGACAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002629
• Insert Size: 1700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002629.2, NP_002620.1
• RefSeq Size: 1762 bp
• RefSeq ORF: 765 bp
• Locus ID: 5223
• UniProt ID: P18669
• Cytogenetics: 10q24.1
• Domains: PGAM
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways
• Gene Summary: The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).