Prolyl Endopeptidase (PREP) (NM_002726) Human Untagged Clone

SKU SC118444

PREP (untagged)-Human prolyl endopeptidase (PREP)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Prolyl Endopeptidase
• Synonyms: PE; PEP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002726 edited; GAATTCGGCACGAGGCTGGCCGTGAGCNGGCCGCCCGCTGCCGCCGGCCGCCCCGCAGCT; GCCTGCGCCCCAGCCGCGCCTCGCGGCCAGCCCGGCTAGCTCAGGTCCGCTCCCGGAGCC; CGCGCCCCTCCACGCTGCCCCCTGCCTGTCCCCGGCCATGCTGTCCCTTCAGTACCCCGA; CGTGTACCGCGACGAGACCGCCGTACAGGATTATCATGGTCATAAAATTTGTGACCCTTA; CGCCTGGCTTGAAGACCCCGACAGTGAACAGACTAAGGCCTTTGTGGAGGCCCAGAATAA; GATTACTGTGCCATTTCTTGAGCAGTGTCCCATCAGAGGTTTATACAAAGAGAGAATGAC; TGAACTATATGATTATCCCAAGTATAGTTGCCACTTCAAGAAAGGAAAACGGTATTTTTA; TTTTTACAATACAGGTTTGCAGAACCAGCGAGTATTATATGTACAGGATTCCTTAGAGGG; TGAGGCCAGAGTGTTCCTGGACCCCAACATACTGTCTGACGATGGCACAGTGGCACTCCG; AGGTTACGCGTTCAGCGAAGATGGTGAATATTTTGCCTATGGTCTGAGTGCCAGTGGCTC; AGACTGGGTGACAATCAAGTTCATGAAAGTTGATGGTGCCAAAGAGCTTCCAGATGTGCT; TGAAAGAGTCAAGTTCAGCTGTATGGCCTGGACCCATGATGGGAAGGGAATGTTCTACAA; CTCATACCCTCAACAGGATGGAAAAAGTGATGGCACAGAGACATCTACCAATCTCCACCA; AAAGCTCTACTACCATGTCTTGGGAACCGATCAGTCAGAAGATATTTTGTGTGCTGAGTT; TCCTGATGAACCTAAATGGATGGGTGGAGCTGAGTTATCTGATGATGGCCGCTATGTCTT; GTTATCAATAAGGGAAGGATGTGATCCAGTAAACCGACTCTGGTACTGTGACCTACAGCA; GGAATCCAGTGGCATCGCGGGAATCCTGAAGTGGGTAAAACTGATTGACAACTTTGAAGG; GGAATATGACTACGTGACCAATGAGGGGACGGTGTTCACATTCAAGACGAATCGCCAGTC; TCCCAACTATCGCGTGATCAACATTGACTTCAGGGATCCTGAAGAGTCTAAGTGGAAAGT; ACTTGTTCCTGAGCATGAGAAAGATGTCTTAGAATGGATAGCTTGTGTCAGGTCCAACTT; CTTGGTCTTATGCTACCTCCATGACGTCAAGAACATTCTGCAGCTCCATGACCTGACTAC; TGGTGCTCTCCTTAAGACCTTTCCGCTCGATGTCGGCAGCATTGTAGGGTACAGCGGTCA; GAAGAAGGACACTGAAATCTTCTATCAGTTTACTTCCTTTTTATCTCCAGGTATCATTTA; TCACTGTGATCTTACCAAAGAGGAGCTGGAGCCAAGAGTTTTCCGAGAGGTGACCGTAAA; AGGAATTGATGCTTCTGATTACCAGACAGTCCAGATTTTCTACCCTAGCAAGGATGGTAC; GAAGATTCCAATGTTCATTGTGCATAAAAAAGGCATAAAATTGGATGGCTCTCATCCAGC; TTTCTTATATGGCTATGGCGGCTTCAACATATCCATCACACCCAACTACAGTGTTTCCAG; GCTTATTTTTGTGAGACACATGGGTGGTATCCTGGCAGTGGCCAACATCAGAGGAGGTGG; CGAATATGGAGAGACGTGGCATAAAGGTGGTATCTTGGCCAACAAACAAAACTGCTTTGA; TGACTTTCAGTGTGCTGCTGAGTATCTGATCAAGGAAGGTTACACATCTCCCAAGAGGCT; GACTATTAATGGAGGTTCAAATGGAGGCCTCTTAGTGGCTGCTTGTGCAAATCAGAGACC; TGACCTCTTTGGTTGTGTTATTGCCCAAGTTGGAGTAATGGACATGCTGAAGTTTCATAA; ATATACCATCGGCCATGCTTGGACCACTGATTATGGGTGCTCGGACAGCAAACAACACTT; TGAATGGCTTGTCAAATACTCTCCATTGCATAATGTGAAGTTACCAGAAGCAGATGACAT; CCAGTACCCGTCCATGCTGCTCCTCACTGCTGACCATGATGACCGCGTGGTCCCGCTTCA; CTCCCTGAAGTTCATTGCCACCCTTCAGTACATCGTGGGCCGCAGCAGGAAGCAAAGCAA; CCCCCTGCTTATCCACGTGGACACCAAGGCGGGCCACGGGGCGGGGAAGCCCACAGCCAA; AGTGATAGAGGAAGTCTCAGACATGTTTGCGTTCATCGCGCGGTGCCTGAACGTCGACTG; GATTCCATAAACAGTTTTCGTGCTTCCTCCTGACAGCGACAGAAAACCTCAAGGGCTTTC; CCACGTTGACACCAAGAAACCACTGGGCATAATGCTTCCCCACGGGAACATTATTCCTGC; ACTCACAGGCTACAGTTGAACAGAACTGCCGTGGGAATTTTATCTTTTTTAGGCTTCTCC; TTTTTAGCAAGGCCTTGGTGTTTCTTTTTCCACCCTGTCTAGGCACATGTGGTTTTTTGG; TGTTTTTTTTAAGGGCATGTTGGGATAAATAGCTAAATGGCAACAAACACATTGTGAATA; TTAGATTGCTGAATTAAGGATCATAGTCGGGCATACTTATCTATATCCATAACCTCTATA; TCTTTAAATAAATGTGAGAACTGTTCTCATGGAGAAGACTTCTTTGCAACAATAATAAAT; GTTATTTAAGAATGAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002726 unedited; GCACAATTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTGGCCGTGAGC; CGGCCGCCCGCTGCCGCCGGCCGCCCCGCAGCTGCCTGCGCCCCAGCCGCGCCTCGCGGC; CAGCCCGGCTAGCTCAGGTCCGCTCCCGGAGCCCGCGCCCCTCCACGCTGCCCCCTGCCT; GTCCCCGGCCATGCTGTCCCTTCAGTACCCCGACGTGTACCGCGACGAGACCGCCGTACA; GGATTATCATGGTCATAAAATTTGTGACCCTTACGCCTGGCTTGAAGACCCCGACAGTGA; ACAGACTAAGGCCTTTGTGGAGGCCCAGAATAAGATTACTGTGCCATTTCTTGAGCAGTG; TCCCATCAGAGGTTTATACAAAGAGAGAATGACTGAACTATATGATTATCCCAAGTATAG; TTGCCACTTCAAGAAAGGAAAACGGTATTTTTATTTTTACAATACAGGTTTGCAGAACCA; GCGAGTATTATATGTACAGGATTCCTTAGAGGGTGAGGCCAGAGTGTTCCTGGACCCCAA; CATACTGTCTGACGATGGCACAGTGGCACTCCGAGGTTACGCGTTCAGCGAAGATGGTGA; ATATTTTGCCTATGGTCTGAGTGCCAGTGGCTCAGACTGGGTGACAATCAAGTTCATGAA; AGTTGATGGTGCCAAAGAGCTTCCAGATGTGCTTGAAAGAGTCAAGTTCAGCTGTATGGC; CTGTACCCATGATGGGAANGGAATGTTCTACAACTCATACCCTCCACAGGATGGGAAAAG; TGATGGCACAGAGACATCTACCAATCTCCACCAAAAGCTCTACTACCATGTCTTGGGGAC; CGATCAGTCAGAAGATATTTTGTGTGCTGAGNTTTCTGATGAACCCTAATGGATGGTGGA; GCTNGAGTATCTGATGATGGCCGCTATGTTCTGTTATCATTAAGAAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002726 unedited; GGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTCCTTAAATAACATTTAT; TATTGTTGCAAAGAAGTCTTCTCCATGAGAACAGTTCTCACATTTATTTAAAGATATAGA; GGTTATGGATATAGATAAGTATGCCCGACTATGATCCTTAATTCAGCAATCTAATATTCA; CAATGTGTTTGTTGCCATTTAGCTATTTATCCCAACATGCCCTTAAAAAAAACACCAAAA; AACCACATGTGCCTAGACAGGGTGGAAAAAGAAACACCAAGGCCTTGCTAAAAAGGAGAA; GCCTAAAAAAGATAAAATTCCCACGGCAGTTCTGTTCAACTGTAGCCTGTGAGTGCAGGA; ATAATGTTCCCGTGGGGAAGCATTATGCCCAGTGGTTTCTTGGTGTCAACGTGGGAAAGC; CCTTGAGGTTTTCTGTCGCTGTCAGGAGGAAGCACGAAAACTGTTTATGGAATCCAGTCG; ACGTTCAGGCACCGCGCGATGAACGCAAACATGTCTGAGACTTCCTCTATCACTTTGGCT; GTGGGCTTCCCCGCCCCGTGGCCCGCCTTGGTGTCCACGTGGATAAGCAGGGGGTTGCTT; TGCTTCCTGCTGCGGCCCACGATGTACTGAAGGGTGGCAATGAACTTCAGGGAGTGAAGC; GGGACCACGCGGTCATCATGGTCAGCAGTGAGGAGCAGCATGGACGGGTACTGGATGTCA; TCTGCTTCTGGTAACTTCACATATGCAATGGAGAGTATTTGACAAGCCATTCAAAGTGTT; GTTTGCTGTCCGAGCACCATAATCAGTGGTCCAGCATGGCCGATGGTATATTATGAAACT; CAGCATGTCCATACNNTCACTGGGCATTACACACCAAGNAGTCAGGTCTCTGATTGCACA; GCAGCACTAAGAGCCCTCATTGAACCTCATATAGTCAGCTTTGGGAGAGGGAACCTNCTG; ATAGACTCANAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_002726
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002726.3, NP_002717.3
• RefSeq Size: 2849 bp
• RefSeq ORF: 2133 bp
• Locus ID: 5550
• UniProt ID: P48147
• Cytogenetics: 6q21
• Domains: Peptidase_S9, Peptidase_S9_N
• Protein Families: Druggable Genome, Protease
• Summary: The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides. [provided by RefSeq, Jul 2008]