PSMC6 (NM_002806) Human Untagged Clone

CAT#: SC118427

PSMC6 (untagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMC6
• Synonyms: p42; RPT5; SUG2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002806, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCATTCCCGGCATCCCCTATGAGAGACGGCTTCTCATCATGGCGGACCCTAGAGATAAGGCGCTTC
  AGGACTACCGCAAGAAGTTGCTTGAACACAAGGAGATCGACGGCCGTCTTAAGGAGTTAAGGGAACAATT
  AAAAGAACTTACCAAGCAGTATGAAAAGTCTGAAAATGATCTGAAGGCCCTACAGAGTGTTGGGCAGATC
  GTGGGTGAAGTGCTTAAACAGTTAACTGAAGAAAAATTCATTGTTAAAGCTACCAATGGACCAAGATATG
  TTGTGGGTTGTCGTCGACAGCTTGACAAAAGTAAGCTGAAGCCAGGAACAAGAGTTGCTTTGGATATGAC
  TACACTAACTATCATGAGATATTTGCCGAGAGAGGTGGATCCACTGGTTTATAACATGTCTCATGAGGAC
  CCTGGGAATGTTTCTTATTCTGAGATTGGAGGGCTATCAGAACAGATCCGGGAATTAAGAGAGGTGATAG
  AATTACCTCTTACAAACCCAGAGTTATTTCAGCGTGTAGGAATAATACCTCCAAAAGGCTGTTTGTTATA
  TGGACCACCAGGTACGGGAAAAACACTCTTGGCACGAGCCGTTGCTAGCCAGCTGGACTGCAATTTCTTA
  AAGGTTGTATCTAGTTCTATTGTAGACAAGTACATTGGTGAAAGTGCTCGTTTGATCAGAGAAATGTTTA
  ATTATGCTAGAGATCATCAACCATGCATCATTTTTATGGATGAAATAGATGCTATTGGTGGTCGTCGGTT
  TTCTGAGGGTACTTCAGCTGACAGAGAGATTCAGAGAACGTTAATGGAGTTACTGAATCAAATGGATGGA
  TTTGATACTCTGCATAGAGTTAAAATGATCATGGCTACAAACAGACCAGATACACTGGATCCTGCTTTGC
  TGCGTCCAGGAAGATTAGATAGAAAAATACATATTGATTTGCCAAATGAACAAGCAAGATTAGACATACT
  GAAAATCCATGCAGGTCCCATTACAAAGCATGGTGAAATAGATTATGAAGCAATTGTGAAGCTTTCGGAT
  GGCTTTAATGGAGCAGATCTGAGAAATGTTTGTACTGAAGCAGGTATGTTCGCAATTCGTGCTGATCATG
  ATTTTGTAGTACAGGAAGACTTCATGAAAGCAGTCAGAAAAGTGGCTGATTCTAAGAAGCTGGAGTCTAA
  ATTGGACTACAAACCTGTGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002806 unedited
  NGTTCGNATATTTGTATACGACTCACTATTAGGGCGGCCGCGAATCGGCACGAGGTCATG
  GCGGACCCTAGAGATAAGGCGCTTCAGGACTACCGCAACTAGTTGCTTGAACACAAGGAG
  ATCGACGGCCGTCTTAAGGAGTTAAGGGAACAATTAAAAGAACTTACCAAGCAGTATGAA
  AAGTCTGAAAATGATCTGAAGGCCCTACAGAGTGTTGGGCAGATCGTGGGTGAAGTGCTT
  AAACAGTTAACTGAAGAAAAATTCATTGTTAAAGCTACCAATGGACCAAGATATGTTGTG
  GGTTGTCGTCGACAGCTTGACAAAAGTAAGCTGAAGCCAGGAACAAGAGTTGCTTTGGAT
  ATGACTACACTAACTATCATGAGATATTTGCCGAGAGAGGTGGATCCACTGGTTTATAAC
  ATGTCTCATGAGGACCCTGGGAATGTTTCTTATTCTGAGATTGGAGGGCTATCAGAACAG
  ATCCGGGAATTAAGAGAGGTGATAGAATTACCTCTTACAAACCCAGAGTTATTTCAGCGT
  GTAGGAATAATACCTCCAAAAGGCTGTTTGTTATATGGACCACCAGGTACGGGAAAAACA
  CTCTTGGCACGAGCCGTTGCTAGCCAGCTGGACTGCAATTTCTTAAAGGTTGTATCTAGT
  TCTATTGTAGACAAGTACATTGGTGAAAGTGCTCGTTTGATCAGAGAAATGTTTAATTAT
  GCTAGAGATCATCAACCATGCATCATTTCTATGGATGAAATAGATGCTATTGGTGTNCGN
  NCCGTTTCTTGAGGGTACTTCAGCTGACAGAGAGATTCAGAGAACGTTAATGGAGTTACT
  GAATCAACTGGATGGATTTGATACTCTGCATAGAGACTAAATGATCATGGCTACAAACAG
  ACCGATACACTCGGATCCTGCTTTGCTGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002806 unedited
  GCGGCCGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGTAGGAGAACTGAATCAAAC
  AGATTTTATTCAACTTTTTAGATGAGGAAAACAAATGATACAAAATAAGTCATAAGAAAT
  GCTTTCTTATACCACTATCTCAAACCACTTTCAATATTTTACAAAATGCTCACGCAGCAA
  ATATGAAAAGCTTCAACACTTTCCCTTTGTAACTTGCTGCAATAAATGCAACTTTAACAA
  ACATACAAATTTCTTCTGTATCTTAAAAGTTGAATTACTAATTTTTATGATGTTACTCAT
  ATTTTTATTCATATACTTTTAATGACATCATTGCCAATACATACATTATTTTCTTTAACT
  TTATTTTTACAATAAGCCAACATCTGTCATGCAGCCATCAAAAATCTTACAGTAAATTAC
  ACAGGTTTGTAGTCCAATTTAGACTCCAGCTTCTTAGAATCAGCCACTTTTCTGACTGCT
  TTCATGAAGTCTTCCTGTACTACAAAATCATGATCAGCACGAATTGCGAACATACCTGCT
  TCAGTACAAACATTTCTCAGATCTGCTCCATTAAAGCCATCCGAAAGCTTCACAATTGCT
  TCATAATCTATTTCACCATGCTTTGTAATGGGACCTGCATGGATTTTCAGTATGTCTAAT
  CTTGCTTGTTCATTTGGCAAATCAATATGGATTTTTCTATCTAATCTTCCTGGACGCAGC
  AAAGCAGGATCCAGTGTATCTGTTCTGTTTGTAGCCATGATCATTTTAACTCTATGCAGA
  GTATCAAATCCCTCCATTTGATTCAGGAACTCCATCAACGTTCTCTGAATCTCTCTGTCA
  GCTGAAGTACCCTCAAGAAACCGGCGACCCACCATATCATCTCATTTCTCCATAAAAAGA
  TGCCTGTCCGAAGAACCCTAGCCTAATTAACATCCCTCTGATCAACGAGCACCTTCCCAA
  TGTCCTGGCCTAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002806
• Insert Size: 1590 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002806.2, NP_002797.2
• RefSeq Size: 1599 bp
• RefSeq ORF: 1170 bp
• Locus ID: 5706
• UniProt ID: P62333
• Cytogenetics: 14q22.1
• Domains: AAA, AAA
• Protein Pathways: Proteasome
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, Jul 2008]