SAPK4 (MAPK13) (NM_002754) Human Untagged Clone

SKU SC118423

MAPK13 (untagged)-Human mitogen-activated protein kinase 13 (MAPK13)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SAPK4
• Synonyms: MAPK-13; MAPK 13; p38delta; PRKM13; SAPK4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002754 edited; ATGAGCCTCATCCGGAAAAAGGGCTTCTACAAGCAGGACGTCAACAAGACCGCCTGGGAG; CTGCCCAAGACCTACGTGTCCCCGACGCACGTCGGCAGCGGGGCCTATGGCTCCGTGTGC; TCGGCCATCGACAAGCGGTCAGGGGAGAAGGTGGCCATCAAGAAGCTGAGCCGACCCTTT; CAGTCCGAGATCTTCGCCAAGCGCGCCTACCGGGAGCTGCTGCTGCTGAAGCACATGCAG; CATGAGAACGTCATTGGGCTCCTGGATGTCTTCACCCCAGCCTCCTCCCTGCGCAACTTC; TATGACTTCTACCTGGTGATGCCCTTCATGCAGACGGATCTGCAGAAGATCATGGGGATG; GAGTTCAGTGAGGAGAAGATCCAGTACCTGGTGTATCAGATGCTCAAAGGCCTTAAGTAC; ATCCACTCTGCTGGGGTCGTGCACAGGGACCTGAAGCCAGGCAACCTGGCTGTGAATGAG; GACTGTGAACTGAAGATTCTGGATTTTGGGCTGGCGCGACATGCAGACGCCGAGATGACT; GGCTACGTGGTGACCCGCTGGTACCGAGCCCCCGAGGTGATCCTCAGCTGGATGCACTAC; AACCAGACAGTGGACATCTGGTCTGTGGGCTGTATCATGGCAGAGATGCTGACAGGGAAA; ACTCTGTTCAAGGGGAAAGATTACCTGGACCAGCTGACCCAGATCCTGAAAGTGACCGGG; GTGCCTGGCACGGAGTTTGTGCAGAAGCTGAACGACAAAGCGGCCAAATCCTACATCCAG; TCCCTGCCACAGACCCCCAGGAAGGATTTCACTCAGCTGTTCCCACGGGCCAGCCCCCAG; GCTGCGGACCTGCTGGAGAAGATGCTGGAGCTAGACGTGGACAAGCGCCTGACGGCCGCG; CAGGCCCTCACCCATCCCTTCTTTGAACCCTTCCGGGACCCTGAGGAAGAGACGGAGGCC; CAGCAGCCGTTTGATGATTCCTTAGAACACGAGAAACTCACAGTGGATGAATGGAAGCAG; CACATCTACAAGGAGATTGTGAACTTCAGCCCCATTGCCCGGAAGGACTCACGGCGCCGG; AGTGGCATGAAGCTGTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002754 unedited; CACCAGGCGGCGCGGGGCGGGCGCAGCGGGGGTCGGGGCGCTGGGAGCCCGTTGGGCCGC; GAACGCAGCCGCCACGCCGGGGCCGCCGAGATCGGGTGCCCGGGATGAGCCTCATCCGGA; AAAAGGCTTCTACAAGCAGGACGTCAACAAGACCGCCTGGGAGCTGCCCAAGACCTACGT; GTCCCCGACGCACGTCGGCAGCGGGGCCTATGGCTCCGTGTGCTCGGCCATCGACAAGCG; GTCAGGGGAGAAGGTGGCCATCAAGAAGCTGAGCCGACCCTTTCAGTCCGAGATCTTCGC; CAAGCGCGCCTACCGGGAGCTGCTGCTGCTGAAGCACATGCAGCATGAGAACGTCATTGG; GCTCCTGGATGTCTTCACCCCAGCCTCCTCCCTGCGCAACTTCTATGACTTCTACCTGGT; GATGCCCTTCATGCAGACGGATCTGCAGAAGATCATGGGGATGGAGTTCAGTGAGGAGAA; GATCCAGTACCTGGTGTATCAGATGCTCAAAGGCCTTAAGTACATCCACTCTGCTGGGGT; CGTGCACAGGGACCTGAAGCCAGGCAACCTGNCTGTGAATGAGGACTGTGAACTGAAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002754 unedited; NAAAATACGTGTACCGCGCCGCTTTCTANGACTCGAGTTTTTTTTTTTTTTGTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGAATTTGCAGGTTTAATTAATAA; GGGTCAAGATAAGATCACCCCGGATTAGGGGGGGCCCTAAATCCAATGACTGTTGTTAAA; AGAGGAGAAAACCGAAAAAACCGAGACTGGGGTGATTCTTCAATAAGCTGGGGAATGCCA; CGGACTGCCCACTGCCGCCACAAGCTAAAAAGAGCCAAGGAAGGATTTTTCCCCAAAGCC; TTTAAAGGGACCACAGCCCTGCCAACACCTCCATCCCAAATTTCCAGCCTCCAAAACTGT; AAGGGAATCAAGTTCTGCTGTTTCAATCCACCAGGTTTGGGGTAATTCGTTAGAGCATCC; CTAGAAAACCAACACAGCATTACTGCCATAGGCCCCCGGCCTGGGTTAACGCCCACTCAG; CCCCACTTTGGCGGGAAGGAAAGGGCCCCACCTGCAGGCGATTCTCCAAATGGGCAGTTT; AACGTGGCCTGTTAGGATCAAAACTAGTTTCTCCCAACCGACATCTTTGAATTTTGCATC; TACTAGGCCCATTTCCCACATAAGGAGAAGGACCCTTCTGTCCTCTGCTTGAAAGGCTGT; ATTCCAAGAAAGGCTGAGTTTGGTAGTGACAAATACTGGTCCTTGGGCAATGTCTGGCCG; GCATGGCCATGCAAAATGAGTCCCTACAGCTTTATGCCACTCCGGCGCCGTGAGTCCTTC; CGGGCAATGGGGCTGAAGTTAAAATCTCCTTGTAGATGTGCCGCTTCCATTCATCCACTG; TGAGTTTCTCGTGTTCTCAGGAATCATCAAAGGCTGCTGGGCCTCCGTCTCTTTCTCAGG; GTCCCGGAAGGGTTCAAAGAAGGGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_002754
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002754.3, NP_002745.1
• RefSeq Size: 1888 bp
• RefSeq ORF: 1098 bp
• Locus ID: 5603
• UniProt ID: O15264
• Cytogenetics: 6p21.31
• Domains: pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Epithelial cell signaling in Helicobacter pylori infection, Fc epsilon RI signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway, VEGF signaling pathway
• Summary: This gene encodes a member of the mitogen-activated protein (MAP) kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The encoded protein is a p38 MAP kinase and is activated by proinflammatory cytokines and cellular stress. Substrates of the encoded protein include the transcription factor ATF2 and the microtubule dynamics regulator stathmin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]; Transcript Variant: This variant (1) represents the longer transcript and is protein-coding. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.